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Raffaele Dall'Olio

Raffaele Dall'Olio

PhD Animal Biology

About

40
Publications
28,106
Reads
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1,109
Citations
Additional affiliations
June 2007 - present
CRA Agricultural Research Council / OWNER BeeSources
Position
  • Sensory Analyses of Honey, Practical Beekeeping, Honeybee pathology
October 2006 - present
CRA-API
Position
  • Honeybee phylogeny, popgenetics, pathology
January 1998 - July 2003
University of Bologna
Education
February 2011 - February 2013
Università di Pisa
Field of study
  • Veterinary Science
September 2000 - May 2003
University of Bologna
Field of study
  • Molecular Biology
September 1995 - July 2000
University of Bologna
Field of study
  • Biology

Publications

Publications (40)
Article
Full-text available
EurBeST — A Pilot Study Testing Varroa-resistant Bees Under Commercial Beekeeping Conditions.
Conference Paper
Full-text available
Different brood interruption methods combined with chemical treatment may successfully remove most V. destructor from the honey bee colonies. Further, the timely brood reduction or break may positively impact nectar intake and is occasionally used by beekeepers during the main spring flow to increase honey production. However, the experience and da...
Method
Full-text available
EURBEST KSIĄŻKA METOD OCENY PSZCZELARZE PROWADZĄCY OCENĘ STACJONARNĄ PT Uzunov et., 2021
Method
Full-text available
EURBEST KSIĄŻKA METOD OCENY PSZCZELARZE PROWADZĄCY OCENĘ TERENOWĄ Uzunov et al., 2021
Article
Full-text available
Citizen Science contributes significantly to the conservation of biodiversity, but its application to honey bee research has remained minimal. Even though certain European honey bee (Apis mellifera) populations are known to naturally survive Varroa destructor infestations, it is unclear how widespread or common such populations are. Such colonies a...
Article
Full-text available
Background With numerous endemic subspecies representing four of its five evolutionary lineages, Europe holds a large fraction of Apis mellifera genetic diversity. This diversity and the natural distribution range have been altered by anthropogenic factors. The conservation of this natural heritage relies on the availability of accurate tools for s...
Article
Full-text available
The socio-economic impacts of COVID-19 on society have yet to be truly revealed; there is no doubt that the pandemic has severely affected the daily lives of most of humanity. It is to be expected that the research activities of scientists could be impacted to varying degrees, but no data exist on how COVID-19 has affected research specifically. He...
Conference Paper
Nowadays the Western honey bee (Apis mellifera) is present worldwide, wherever beekeeping is possible, though its native habitat spreads out across Europe, Africa and parts of Western Asia. About 26 different subspecies evolved through time, each adapted by natural selection to cope with the local environmental conditions. During the past 2 centuri...
Article
Full-text available
More than 30 years after the first infestation report on Apis mellifera, varroa mites are still damaging honey bee health. The present paper reports data from a field experiment concerning two early spring (late February) bio-technical management techniques: early brood interruption (through queen caging) and early trapping comb (through the use of...
Article
Full-text available
Honey bees are pollinators that play a key-role in plant biodiversity conservation and crop production. This unique insect species has been managed in hives by beekeepers for millennia, even though such a peculiar animal production system never resulted in the domestication of the western honey bee. The western honey bee was originally distributed...
Article
Nosema ceranae is now a widespread honey bee pathogen with high incidence in apiculture. Rapid and reliable detection and quantification methods are a matter of concern for research community, nowadays mainly relying on the use of biomolecular techniques such as PCR, RT-PCR or HRMA. The aim of this technical paper is to provide a new qPCR assay, ba...
Conference Paper
Full-text available
Neonicotinoids have been among the most frequently used insecticides in the cultivation of several crops and orchards. Till 2012, imidacloprid represented 41.5% of the whole neonicotinoid market and thiamethoxam was the second biggest neonicotinoid, followed by clothianidin. However, almost two years ago, a decision was made by the EC parliament to...
Conference Paper
Full-text available
The genetic variability of the A. m. sicula and ligustica are today evaluated by nuclear and mitochondrial markers. The aim of this work was to create a reference database to classify individuals of unknown origin as belonging to one of the two subspecies or hybrids. The DNA was extracted from the legs of 54 samples of A. m. ligustica and 209 of A....
Article
Full-text available
1. Apis mellifera siciliana is a honey bee subspecies prone to be con-served. Conservation measures include maintaining colonies on different islands to serve as parental lines for outcrossing. 2. Molecular diversity and genetic structure of honey bees from Sicily (main island) and Vulcano and Filicudi (conservation islands) were analysed with mito...
Article
Full-text available
The recognition that the Dark European honey bee, Apis mellifera mellifera, is increasingly threatened in its native range has led to the establishment of conservation programmes and protected areas throughout western Europe. Previous molecular surveys showed that, despite management strategies to preserve the genetic integrity of A. m. mellifera,...
Conference Paper
Full-text available
A project is ongoing to protect and increase the population of the autochthonous Sicilian black bee Apis mellifera siciliana. The field activities involve rearing and distribution of queen cells. Breeding lines and suitability of mating areas is assessed with molecular tools. Ten microsatellite loci are analysed with a multiplex -PCR technique. Thi...
Article
Full-text available
A comparison among four different summer strategies for the control of Varroa destructor was carried out from summer 2010 until spring 2011, in collaboration with national beekeepers associations in Italy. A total of 170 colonies located in Lazio, Piedmont, Tuscany, Veneto were involved. The control strategies included chemical treatments and biote...
Chapter
Analogamente alle altre specie animali di interesse zootecnico, domestiche o selvatiche, le api possono essere affette da un’ampia varietà di patogeni infettivi responsabili di mortalità delle colonie. Tra questi anche i virus, che sono considerati con sempre maggior preoccupazione dagli apicoltori per la loro capacità di compromettere la salute de...
Conference Paper
Full-text available
The subspecies Apis mellifera siciliana, of Sicily, is threatened with extinction due to hybridization with A. m. ligustica, from 1970. Based on distribution of large numbers of A. m. siciliana queen mothers to beekeepers in the Western part of the island is current a project about the reintroduction of the subspecies. Here we present preliminary d...
Article
Full-text available
Honey bee diversity is under threat due to anthropogenic factors as the use of pesticides and the replacement of local colonies to recover from colony losses. To assess the effect of these activities on the genetic diversity and structure of North African honey bee colonies, we studied colonies from the north (Tellian) and the south (Saharan) regio...
Article
Full-text available
review the methodology applied in each country for discriminating between honey bee populations. Morphometric analyses (classical and geometric) and different molecular markers have been applied. Even if the approach has been similar, however, different methodologies regarding measurements, landmarks or molecular markers may have been used, as well...
Article
Full-text available
The genetic structure and molecular diversity of Croatian honeybee coastal populations have been investigated with microsatellite and mitochondrial markers. According to sequence data of the mitochondrial tRNAleu-cox2 intergenic region, all analysed samples belong to the Central Mediterranean and Southeast European evolutionary C-lineage. Four mito...
Article
Although pollinator declines are a global biodiversity threat, the demography of the western honeybee (Apis mellifera) has not been considered by conservationists because it is biased by the activity of beekeepers. To fill this gap in pollinator decline censuses and to provide a broad picture of the current status of honeybees across their natural...
Article
Full-text available
AbstractThe distribution of various evolutionary lineages of Apis mellifera subspecies in Africa is still controversial. We sampled honeybees from eight coastal locations and three Saharan oases in Libya and analyzed mtDNA variability with restriction fragment length polymorphisms and the sequence of the tRNAleu-cox2 intergenic region. Haplotypes b...
Article
Full-text available
Europe harbours several endemic honeybee (Apis mellifera) subspecies. Yet the distribution of these subspecies is nowadays also much influenced by beekeeping activities. Large scale migratory beekeeping and trade in queens, coupled with the promiscuous mating system of honeybees, have exposed native European honeybees to increasing introgressive h...
Article
Full-text available
The genetic variability of Apis mellifera ligustica was screened throughout the Italian peninsula and Sardinia with eight polymorphic microsatellite loci. Samples of Apis mellifera mellifera, Apis mellifera carnica and from the Buckfast breeding line were genotyped for comparison. Low Fis and Fst values suggest the absence of genetic structure with...
Article
Full-text available
Batrachochytrium dendrobatidis (phylum Chytridiomycota, order Chytridiales) is the causative organism of chytridiomycosis in amphibians, a disease associated with their population decline worldwide. In this work, we report a cutaneous infection in water frogs of the Rana esculenta complex in agricultural areas of Umbria, central Italy. Histological...
Article
Full-text available
The first results of the monitoring, from 2000 to 2002, and conservation of a few yellow‐bellied toad populations found in the northern parts of the Apennines are presented. In the province of Bologna this species is particularly threatened, while around the region of Romagna it appears to be less so. Some factors were identified that could have a...

Questions

Questions (3)
Question
Bulletin of Insectology 70 (2): 261-265, 2017 ISSN 1721-8861 Detection of deformed wing virus in Vespa crabro
Question
During artificial Insemination in Honeybee queens, someone uses chloroform to help endophallus eversion process in drones. Does anybody know if there is any paper concerning chloroform affecting sperm vitality (in any species.. not honeybee only)?

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Cited By

Projects

Projects (5)
Project
The EurBeST study explores possibilities for increasing the varroa resistance of commercially available honey bees by selective breeding and analyses ways to improve beekeepers’ access to resistant material. www.eurbest.eu
Project
B-GOOD will pave the way towards healthy and sustainable beekeeping within the European Union by following a collaborative and interdisciplinary approach. Merging data from within and around beehives as well as wider socioeconomic conditions, B-GOOD will develop and test innovative tools to perform risk assessments according to the Health Status Index (HSI). B-GOOD has the overall goal to provide guidance for beekeepers and help them make better and more informed decisions. This project receives funding from the European Union's Horizon 2020 research and innovation programme under grant agreement No 817622.