Rafaela Cuenca

Rafaela Cuenca
Autonomous University of Barcelona | UAB · Department of Medicine and Animal Surgery

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66
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Publications

Publications (66)
Article
Background Quality control procedures are an important part of the overall quality assurance for production of accurate and reliable hematologic results. Objectives This study aimed to validate a quality control material‐based procedure and assess two patient‐based quality control procedures (repeat patient testing [RPT] and average of normals [Ao...
Article
Full-text available
The aim of this study was to determine p-nitrophenyl acetate esterase activity (EA) and cortisol concentrations in serum of marine Olive Ridley turtles (Lepidochelys olivacea) from a Mexican Pacific population (La Escobilla beach) and to evaluate the possible relation of metal and metalloids with these biomarkers. EA, cortisol and selected chemical...
Article
Background: The clinical importance of hematologic testing in avian veterinary medicine is reflected in the increasing number of studies for the establishment of hematologic RIs of Strigiformes and other species. Age is an important physiologic factor in birds and the effect on hematology variable should be understood. Objectives: The objective...
Article
Our objectives were to evaluate the effects of the 1) season, and 2) postmortem changes on serum biochemistries related with metabolism in Pyrenean chamois (Rupicapra pyrenaica pyrenaica). Serum samples from 98 animals obtained from 2009 to 2012 were included. To investigate seasonal influences on blood parameters, the Pyrenean chamois were capture...
Article
Background Establishing reference intervals (RI) for hematologic and biochemical variables in wild animals presents great challenges because capture stress or anesthesia during sampling can affect blood variables.Objectives The aims of this study were to establish RI for hematologic and blood biochemistry variables for Wild Boar (Sus scrofa) caught...
Article
Brachycephalic dogs have unique upper respiratory anatomy with abnormal breathing patterns that are similar to those in humans with obstructive sleep apnea syndrome (OSAS). The objectives of this multicenter prospective study were to assess the effects of surgical correction on clinical signs in dogs with brachycephalic airway obstructive syndrome...
Article
Abstract Our study focuses on the Iberian ibex from the Sierra Nevada Natural Space (southern Spain), where sarcoptic mange is an endemic disease and animals are affected by a highly seasonal environment. Our aim was to distinguish between disease and environmental influences on seasonal variation in body weight, hematology, and serum biochemistry...
Article
The effects of mineral deficiencies are often sub-clinical, and the importance of mineral status is often underestimated in wildlife populations. To our knowledge, this is the first study that gives reference intervals of hepatic minerals for Pyrenean chamois (Rupicapra pyrenaica). We determined macro and trace mineral concentrations in liver sampl...
Article
Full-text available
La interpretación de los parámetros de bioquímica sanguínea en los reptiles es, en bastantes aspectos, parecida a la de los mamíferos. Sin embargo, hay que tener en cuenta que, puesto que los reptiles poseen distintos sistemas de control sobre sus mecanismos homeostáticos a las aves y los mamíferos, los intervalos de normalidad de los diferentes pa...
Article
Full-text available
Background Brachycephalic dogs have unique upper respiratory anatomy with abnormal breathing patterns similar to those in humans with obstructive sleep apnea syndrome (OSAS). The objective of this study was to evaluate the correlation between anatomical components, clinical signs and several biomarkers, used to determine systemic inflammation and m...
Article
Full-text available
The aim of this study was to evaluate the use of whole-body plethysmography as a non-invasive method to determine the respiratory parameters and profiles in two tortoise species belonging to the genus Testudo. Pulmonary functions and volumetric parameters were determined in 10 adults of Testudo hermanni and in seven Testudo marginata animals, using...
Article
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From 2004 to 2007, blood samples from 273 healthy wild boars (Sus scrofa), culled during the hunting season, were obtained in three areas of Catalonia (NE Spain): Pyrenees, Sant Llorenç del Munt i l’Obac Natural Park (SLM), and Ports de Tortosa i Beseit National Hunting Reserve (PTB). We investigated the presence of antibodies against classical swi...
Article
In 2005 and 2006 an outbreak of disease associated with border disease virus (BDV) infection caused high mortality in the Pyrenean chamois (Rupicapra pyrenaica) in the Catalan Pyrenees (NE Spain). The aim of this study was to determine values for different haematological and serum biochemical analytes in 32 free-ranging Pyrenean chamois affected by...
Article
Serum levels of gonadal steroid hormones, corticosterone and thyroxin (T(4)), were monitored monthly in two male and one female captive Loggerhead sea turtles (Caretta caretta) over a period of 12 months in 2004 and 3 months in 2006. Ovary ultrasonography was performed in April and July 2006. The turtles were kept together in an outdoor sea pool in...
Article
Full-text available
Hematology and blood cytology in reptiles. Reptiles are a group of animals ever more common in veterinary practice. In these species, many diagnostic tools are in descriptive stages, includingdiagnostic blood cytology or hematology. This article analyzes blood cells described in reptiles: erythrocytes, heterophiles, eosinophiles, monocytes,azurophi...
Article
Full-text available
Reptiles are a group of animals ever more common in veterinary practice. In these species, many diagnostic tools are in descriptive stages, including diagnostic blood cytology or hematology This article analyzes blood cells described in reptiles: erythrocytes, heterophiles, eosinophiles, monocytes, azurophiles and thrombocytes. It also shows the us...
Article
BRUCELLOSIS is found in domestic and feral pigs around the world, and also in wild boar ( Sus scrofa ), and can be transmitted to human beings. It is considered to be a re-emerging disease in domestic pigs in Europe due to the increasing number of outbreaks on pig farms, often associated with
Article
To study the effects of a synthetic, dog-appeasing pheromone (sDAP) on the behavioral, neuroendocrine, immune, and acute-phase perioperative stress responses in dogs undergoing elective orchiectomy or ovariohysterectomy. Randomized, controlled clinical trial. 46 dogs housed in animal shelters and undergoing elective orchiectomy or ovariohysterectom...
Article
Full-text available
El estudio hematológico y bioquímico ha permitido describir los tipos celulares sanguíneos de la especie y ha revelado que estos lagartos soportan grados de deshidratación inusuales en otras especies de saurios continentales. También ha permitido identificar hemoparásitos presentes en animales aparentemente sanos.
Article
A semiautomatic electronic blood cell counter (Sysmex F-800: Toa Medical Electronics Europa Gmbh, Hamburg, Germany) was evaluated using canine and feline blood, following the International Committee for Standardization in Hematology protocol (ICSH, 1984). Precision and overall reproducibility were acceptable for all the parameters studied except fo...
Article
The aim of this trial was to describe the behavioural, neuroendocrine, immune and acute phase stress responses in dogs undergoing elective surgery in normal, clinical practice conditions. Sixteen dogs were submitted to elective orchiectomy or ovariohysterectomy using a standardised surgical protocol. Each animal was confined to the Intensive Care U...
Article
The aim of this study was to identify ultrasound accessible blood vessels in the loggerhead sea turtle (Caretta caretta) and describe their Doppler waveform patterns, peak systolic velocity, mean velocity, systolic/diastolic ratio as well as pulsatility and resistive indices. B-mode, colour and pulsed-wave Doppler examinations were performed on 10...
Article
Ingesta passage times of soft flat foam dishes and gastric emptying time of barium-impregnated polyethylene spheres (BIPS) were measured in 22 and 8 loggerhead sea turtles (Caretta caretta), respectively. Transit time (T(1)) was considered as the time between ingestion and first elimination, and retention time (T(50)) and total transit time (T(85))...
Article
Full-text available
This study describes four cases of loggerhead sea turtles with fishhooks in the gastrointestinal tract. Two dead turtles with a hook in the esophagus had local fibrosis with an invagination of the keratinized stratified squamous epithelium surrounding the hook, isolating it from the subjacent stroma, one had a hook in the cloaca which was expelled...
Article
Full-text available
This study aimed to provide the normal radiographic anatomic appearance of the limbs of the loggerhead sea turtle, Caretta caretta. Dorsopalmar and dorsoplantar radiographs were taken of the forelimbs and hindlimbs of 15 juvenile and 15 subadult loggerhead sea turtles, 17 alive and 13 dead. For comparison, computed tomographic, gross anatomic, oste...
Article
The aim of this study was to determine the normal computed tomography (CT) appearance of the vertebral column and coelomic structures of the loggerhead sea turtle (Caretta caretta) and to use three-dimensional (3D) and multiplanar reconstructions to indicate the position of each organ in relation to the vertebrae and carapace. Transverse sections o...
Article
Full-text available
Twenty live and five dead juvenile and subadult loggerhead sea turtles were examined ultrasonographically. Ten soft tissue areas of the integument were used as acoustic windows: cervical-dorsal and cervical-ventral, left and right cervicobrachial, left and right axillary, left and right prefemoral and left and right postfemoral windows. Anatomical...
Article
Eosinophilic synovitis (ES) is a rare disease described in human and veterinary medicine. Only three cases have been reported in the horse. A case of tarsocrural synovitis in an Hispano-Arabian gelding is presented in this report. The patient presented with severe joint effusion and lameness of the right tarsocrural joint on admission. Synovial flu...
Article
Full-text available
The Xisqueta sheep breed, located in the Pyrenean of Lleida and adjacent area of Huesca, is characterized by its good adaptation to the conditions of high mountain. In the last decades, many economic and social factors have influenced negatively in the livestock farmer sector, particularly in the Xisqueta breed, being at the moment in extinction da...
Article
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La raza Xisqueta, localizada en el Pirineo de Lleida y zona colindante de Huesca, se caracteriza por su buena adaptación a las condiciones de alta montaña. En las últimas décadas diferentes factores económicos y sociales han influido negativamente en el sector ganadero, particularmente en la raza Xisqueta, encontrándose actualmente en peligro de ex...
Article
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Many investigators have undertaken radiologic studies in chelonians. However, descriptive papers focusing on the radiographic anatomy are limited to only a few species. The purpose of this article is to provide the normal cervical and coelomic radiographic appearance of the loggerhead sea turtle (Caretta caretta), in the dorsoventral view, and to i...
Article
Many investigators have undertaken radiologic studies in chelonians. However, descriptive papers focusing on the radiographic anatomy are limited to only a few species. The purpose of this article is to provide the normal cervical and coelomic radiographic appearance of the loggerhead sea turtle (Caretta caretta), in the dorsoventral view, and to i...
Article
Full-text available
To compare cross-sectional anatomic specimens with images obtained via magnetic resonance imaging (MRI) of the coelomic structures of loggerhead sea turtles (Caretta caretta). 5 clinically normal live turtles and 5 dead turtles. MRI was used to produce T1- and T2- weighted images of the turtles, which were compared with gross anatomic sections of 3...
Article
Full-text available
The object of this study was to examine the erythrocytes, leukocytes and thrombocytes of the giant lizard of El Hierro (Gallotia simonyi) by light and electron (TEM) microscopy, and cytochemical staining. Smears were prepared from blood from the ventral coccygeal vein of 10 healthy adult lizards (five males and five females) from the Giant Lizard o...
Article
Full-text available
Blood samples were taken from the ventral coccygeal vein of 15 El Hierro giant lizards (Gallotia simonyi) (seven females and eight males), six La Gomera giant lizards (Gallotia bravoana) (four males and two females) and four Tenerife giant lizards (Gallotia intermedia) (two males and two females), and 31 blood parameters were measured. Among the ha...
Article
Full-text available
Blood samples were taken from the ventral coccygeal vein of 15 El Hierro giant lizards (Gallotia simonyi) (seven females and eight males), six La Gomera giant lizards (Gallotia bravoana) (four males and two females) and four Tenerife giant lizards (Gallotia intermedia) (two males and two females), and 31 blood parameters were measured. Among the ha...
Article
Hematology and serum biochemistry values can be useful for evaluating the health of populations of captive and free-living European brown hares (Lepus europaeus). However, current information is fragmented and incomplete. The objective of this study was to determine normal hematology and serum chemistry values for a population of captive European b...
Article
To evaluate the cytological and microbiological contents of guttural pouch washes of ten randomly selected horses restrained so as to prevent them lowering their heads, and to assess the possible effects on the guttural pouch environment in these horses. Cytological and microbiological studies were performed on guttural pouch washes of ten clinical...
Conference Paper
Full-text available
Las actuales razas de asnos, según la mayoría de autores, provendrían de dos tipos fundamentales, el Equus asinus africanus y el Equus asinus europeus. Cuatro de las cinco razas estudiadas: Asno de las Encartaciones, Catalana, Mallorquina, y Zamorano-Leonesa, descenderían del tronco europeo, mientras que la raza Andaluza sería originaria del africa...
Article
Guttural pouch lavages were obtained from slaughtered and live horses with use of a percutaneous approach through the Viborg's triangle. Lavage was effective and easy to perform in live horses, and no adverse reactions were observed. Tranquilizers were not required. Recovery of lavage fluid ranged between 25% and 75% of the injected sample in live...
Article
Full-text available
Percutaneous washes of the guttural pouches were obtained from two groups of 15 clinically normal horses, one lightly exercised and the other heavily exercised. Microbiological and cytological studies showed a wide variation in the differential cell counts. The cytological pattern of the normal lavages (< 5 per cent neutrophils) was characterised b...
Article
Full-text available
In an effort to define the effect(s) of lithium heparin and tripotassium EDTA on various hematologic parameters in Testudo hermanni, we collected blood samples from 29 clinically healthy adult individuals of both sexes. Portions of each blood sample were transferred to tubes containing either lithium heparin or tripotassium EDTA. The following dete...
Article
A semiautomatic electronic blood cell counter (Sysmex F-800) was evaluated with equine blood, according to the protocol of the International Committee for Standardization in Haematology (ICSH, 1984). The precision and overall reproducibility were acceptable for all the parameters studied except for the platelet count, in which a coefficient of vari...
Article
Full-text available
Twelve clinical biochemical parameters were determined in 97 animals of all age groups and both sexes of the endangered Catalonian donkey breed. Significant sex differences were observed for phospholipid concentration (P<0.01). Evaluating the effect of management practices on the various parameters showed significant differences for total bilirubin...
Article
Systemic aspergillosis was diagnosed in a two-and-a-half-year-old spayed German shepherd dog which had suffered an acute attack of paralysis of the pelvic limbs. The neurological deficits were attributed to the destruction of the seventh vertebral body and the intervertebral disc, with protrusion of necrotic material into the vertebral canal and co...
Article
1.1. The hematology and erythrocyte dimensions of 11 Montagu's harrier (Circus pygargus) have been studied.2.2. Significant statistical differences between the male and female groups were only detected for MCHC (P < 0.05), length of nucleus (P < 0.01) and cellular surface (P < 0.05).3.3. Both sexes have similar values of erythrocyte number, hematoc...
Article
Haematological and blood biochemical parameters of 15 capercaillie {Tetrao urogallus) reared in captivity have been determined. Statistically significant age differences were observed in the following parameters: haemoglobin, MCHC, total bilirubin, triglycerides, alkaline phosphatase, potassium and total proteins.
Article
1.1. This study deals with the hematological and blood chemistry of 13 adult marsh harriers (Circus aeruginosus).2.2. No significant differences were observed between male and female groups in any of the parameters.3.3. The value of white blood cells was 14,677/mm3 heterophils and lymphocytes, these being the most abundant cellular type (81.42 and...
Article
The agar dilution method was used to determine the inhibitory activities of 28 antimicrobial agents against 35 strains of the genus Peptostreptococcus, 4 strains of the species Peptococcus niger, 20 strains of the species Megasphaera elsdenii, 7 strains from the species Acidaminococcus fermentans, 8 strains of the genus Clostridium, 11 strains of t...
Article
The haematological parameters of healthy great bustards (Otis tarda L.) have been determined. The values obtained were red cell count (3.0 x 10(12) +/- 0.2 x 10(12/)1), white cell count (33.0 x 10(9) +/- 2.6 x 10(9)/1), haematocrit value (0.51 +/- 0.01 1/1), haemoglobin (13.0 +/- 0.3 g/dl), mean corpuscular volume (178.7 +/- 12.5 fl), mean cell hae...
Article
The agar dilution method was used to determine the bacteriostatic activity of 28 antimicrobial agents against 141 strains to the genus Bacteroides and 29 strains from the genus Fusobacterium. All organisms were isolated from clinical cases of ovine footrot. The strains were isolated from 125 Merino sheep, over a period of 2 years, from January 1987...
Article
Lesions showing clinical signs of foot-rot from 120 goats were cultured on six selective media during October 1987 to November 1988. A total of 582 strictly anaerobic microorganisms belonging to 50 different species was isolated and identified. The anaerobes most frequently isolated belonged to the following genera: Bacteroides (80%), Peptostreptoc...
Article
A microbiological study was made of 125 Merino sheep showing clinical signs of foot rot. A total of 435 strictly anaerobic strains were isolated, belonging to the following genera: Bacteroides, Peptostreptococcus, Tissierella, Fusobacterium, Megasphaera, Eubacterium, Acidaminococcus, Clostridium, Peptococcus and Propionibacterium. Of the 35 species...
Article
The agar dilution method was used to determine the antimicrobial activity of 17 antimicrobial agents against 132 strains belonging to the genus Bacteroides and 25 strains from the genus Fusobacterium, all isolated from 120 clinical cases of caprine footrot between October 1987 and November 1988. Josamycin, chloramphenicol and rifampin proved to be...
Article
The agar dilution method was used to determine the bacteriostatic activities of 10 beta-lactam antibiotics against 132 strains belonging to the genus Bacteroides and 25 strains belonging to the genus Fusobacterium, all isolated from clinical cases of caprine foot rot. The three ureidopenicillins studied proved to be the most effective antimicrobial...

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