Rafael Seoane

Rafael Seoane
University of Santiago de Compostela | USC · Department of Microbiology and Parasitology

PhD

About

71
Publications
2,145
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461
Citations
Citations since 2017
10 Research Items
113 Citations
20172018201920202021202220230510152025
20172018201920202021202220230510152025
20172018201920202021202220230510152025
20172018201920202021202220230510152025

Publications

Publications (71)
Article
Full-text available
The efficacy of intraperitoneal injection of an oil-based bivalent autogenous vaccine and the commercial vaccine AlphaJect 3000 (Pharmaq AS) to prevent atypical furunculosis and vibriosis in turbot was analyzed. The effect of both vaccines on health parameters and survival of fish after challenge with V. anguillarum and A. salmonicida subsp. achrom...
Article
Full-text available
Citation: Manuel, R.-P.; Tania, G.-G.; Rafael, S.-P.; Antonio, P.-E.; José, B.-M.; Asteria, L.-Á.; Pablo, C.-B.; Alejandro, L.-P.; Pablo, Á.-N.; Benjamín, M.-B. In Abstract: This study aimed to evaluate the in vitro performance of collagen-based sponges as a drug delivery system for intracanal antimicrobial administration. Four groups of loaded col...
Article
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This study evaluated the reliability of MALDI-TOF MS coupled with statistical tools for the identification of Streptococcus mutans in comparison with PCR-based techniques. Bacterial isolates were identified and serotyped by conventional PCR, using S. mutans species and serotype-specific primers. For bacterial identification, mass spectra data from...
Article
Full-text available
The aim of this study was to determine the prevalence of Streptococcus mutans and its serotypes in samples from oral cavity of young Galician population and their relationship with the oral health state. The variables generally associated with dental caries, such as salivary flow rate, buffering capacity, eating habits, and lifestyle, were also ana...
Article
This paper aimed to design collagen sponges containing tetracycline-loaded PLGA-zein microparticles with controlled release characteristics as an alternative for periodontal treatment. Tetracycline microparticles were prepared by spray-drying technique, while the sponges were prepared by their dispersion in collagen suspension and subsequent freeze...
Article
A new class of amphipathic cyclic peptides, which assemble in bacteria membranes to form polymeric supramolecular nanotubes giving them antimicrobial properties, is described. The method is based on the use of two orthogonal clickable transformations to incorporate different hydrophobic or hydrophilic moieties in a simple, regioselective, and diver...
Article
Full-text available
Three different bioadhesive gels were evaluated in a double-blind randomized clinical trial in which microbial growth in the suture thread was assessed following post-surgical application of the aforementioned gels. Also assessed in this trial were, the intensity of post-surgical pain as well as the degree of healing of the patients’ surgical wound...
Article
The reaction of 3-(aryl)-2-sulfanylpropenoic acids [H2xspa; x: p=3-phenyl-, f=3-(2-furyl)-, t=3-(2-thienyl)-] with methanol or ethanol gave the corresponding methyl (Hxspme) or ethyl (Hxspee) esters. The reaction of these esters (HL) with triphenyltin(IV) hydroxide gave compounds of the type [SnPh3L], which were isolated and characterized as solids...
Article
Objectives: identify by polymerase chain reaction technique ten bacterial species obtained from necrotic root canals, and analyze their association with signs and symptoms of apical periodontitis (pain, exudate and mobility) in a specific geographic area: Santiago de Compostela (northwestern Spain). Methods: a descriptive study was conducted based...
Article
Research into maximizing the speed, precision, and reliability of estimating the postmortem interval (PMI) has been a recurring object of investigation and methodologies based on the vitreous humor (VH) have provided good results. However, contamination from causes not readily apparent, such as blood, can occur, and thus lead not only to an erroneo...
Article
Full-text available
Reaction of NaAuCl4·H2O and thiodiglycol (1:3 molar ratio) with 3-(aryl)-2-sulfanylpropenoic acids, H2xspa = [x:p = 3-phenyl-, f = 3-(2-furyl)-, t = 3-(2-thienyl)-, o-py = 3-(2-pyridyl)-, Clp = 3-(2-chlorophenyl)-, -o-mp = 3-(2-methoxyphenyl)-, -p-mp = 3-(4-methoxyphenyl)-, -o-hp = 3-(2-hydroxyphenyl)-, -p-hp = 3-(4-hydroxyphenyl)-, diBr-o-hp = 3-(...
Article
Full-text available
Treatment with antibiotics within the periodontal pocket against bacterial infections represents a useful and adjunctive tool to conventional therapy for healing and teeth preservation. With this function in view, an implantable, tetracycline delivery device for the treatment of periodontal disease was developed. The aim of this study was to develo...
Article
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The aim of this study was to develop polymeric biodegradable microspheres (MSs) of poly(D-L lactide-co-glycolide) (PLGA) and zein capable of delivering amoxicillin (AMX) at significant levels for root canal disinfection. PLGA/zein MSs were prepared using a spray-drying technique. The systems were characterized in terms of particle size, morphology,...
Article
Two previously unreported thyrotropin (TSH) receptor mutations, A623F and I635V, were identified in toxic follicular thyroid adenoma specimens from two patients with hyperthyroidism. Our aim was to characterize both novel mutants in terms of the following: cAMP basal constitutive activity, cAMP response to TSH, plasma membrane expression levels, an...
Article
Abstract In an attempt to elucidate the biopharmaceutical and clinical interest of new formulations of cyclosporin A (CyA) based on poly(d,l-lactide-co-glycolide) (PLGA) micro- and nanospheres, different in vivo studies were performed. On the one hand, tritium-labeled CyA was loaded into the microspheres (30 and 1 μm) and nano-spheres (0.2 μm) or d...
Article
We investigated the reactions of silver nitrate with 3-(substituted phenyl)-2-sulfanylpropenoic acids H(2)L [L=xspa, where spa=2-sulfanylpropenoato and xin{Clp=3-(2-chlorophenyl)-, -o-mp=3-(2-methoxyphenyl)-, -o-hp=3-(2-hydroxyphenyl)-, -p-hp=3-(4-hydroxyphenyl)-, diBr-o-hp=3-(3,5-dibromo-2-hydroxyphenyl)-}] in 1:1 and 2:1 molar ratios. The 1:1 rea...
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To investigate the mechanisms by which the hypothalamic peptide GHRH influences cell division, we analyzed its effects on the proliferation of two different cell lines: CHO-4, an ovary-derived cell line, and GH3, a pituitary-derived cell line. We found that GHRH induces the proliferation of pituitary-derived cells but inhibits the proliferation of...
Article
We have investigated the reactions of silver nitrate and 3-(aryl)-2-sulfanylpropenoic acids [H(2)xspa, x: p = 3-phenyl-, f = 3-(2-furyl)-, t = 3-(2-thienyl)-, py = 3-(2-pyridyl)-] and 2-cyclopentylidene-2-sulfanylacetic acid (H(2)L) in 1 : 1 and 2 : 1 molar ratios. The 1 : 1 molar ratio gave compounds of type [Ag(HL)]; reaction of these compounds w...
Article
The presenl work describes flow cytometry based methods for Ihe measuremenl o/ phagocytic activity and respiratory burst o/ head-kidney leucocytes of turbot (Scophthalmus maximus) and to characterize Ihe leucocytes subpopulations. Optimal conditons for leucocyte collection and analysis as well as for study o/ phagocytic and respiratory burst activi...
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Undifferentiated (anaplastic) thyroid carcinoma is a highly aggressive human cancer with very poor prognosis. Although there have been a few studies of candidate treatments, the fact that it is an infrequent tumor makes it very difficult to design clinical trials. A strong association has been observed between undifferentiated thyroid carcinoma and...
Article
Pyridoxal 5′-phosphate (PLP) and pyridoxal (PL) itself were reacted with diorganotin(IV) derivatives in the presence and absence of aminoacids. With PLP the complexes [SnR2(PLP-2H)] (R=Me, Et, Bu) were isolated and characterized by EI and FAB mass spectrometry and by IR, Raman and Mössbauer spectroscopy. Reaction mixtures containing PL, valine or g...
Article
This study investigated the binding properties of two monoclonal antibodies (mAbs US1 and US2) raised in (CBA/n x BALB/c)F1 (NBF1) Btk(xid) male mice. Both mAbs show unusual specificity for phosphorylcholine (PC)-containing TSL4 antigens of Trichinella. Specifically, and in contrast to mAbs raised in normal mice, US1 and US2 mAbs do not bind to art...
Article
The compounds [SnR2X2(Hmimt)] (R = Et, Bu; X = Cl, Br; Hmimt = 1-methyl-2(3H)-imidazolinethione) and [SnEt2X2(Hmimt)2] (X = Cl, Br) have been prepared and characterized by elemental analysis, mass spectrometry and IR, Raman, Mössbauer and NMR (1H, 13C and 119Sn) spectroscopy. An X-ray study of the crystal structure of [SnEt2Cl2(Hmimt)2] shows all-t...
Article
The compounds [SnR2X2(Hmimt)] (R = Et, Bu; X = Cl, Br; Hmimt = 1-methyl-2(3H)-imidazolinethione) and [SnEt2X2(Hmimt)2] (X = Cl, Br) have been prepared and characterized by elemental analysis, mass spectrometry and IR, Raman, Mössbauer and NMR (1H, 13C and 119Sn) spectroscopy. An X-ray study of the crystal structure of [SnEt2Cl2(Hmimt)2] shows all-t...
Article
Three new triphenyltin(IV) sulfanylcarboxylates with the general formula [Q][SnPh3(L)] (Q = di-isopropylammonium cation; L = tspa, pspa or pyspa, where t = 3-(2-thienyl)-, p = 3-(2-phenyl)-, py = 3-(2-pyridinyl)- and spa = 2-sulfanylpropenoato) have been prepared by reaction of triphenyltin(IV) hydroxide with the corresponding acid in the presence...
Article
Three new triphenyltin(IV) sulfanylcarboxylates with the general formula [Q][SnPh3(L)] (Q = di-isopropylammonium cation; L = tspa, pspa or pyspa, where t = 3-(2-thienyl)-, p = 3-(2-phenyl)-, py = 3-(2-pyridinyl)- and spa = 2-sulfanylpropenoato) have been prepared by reaction of triphenyltin(IV) hydroxide with the corresponding acid in the presence...
Article
Background Translocation of bacteria from the gut to other organs is one cause of systemic complications in obstructive jaundice. Furthermore, hyperbilirubinaemia appears to cause anergy. This study investigated whether these responses (translocation of gut bacteria and anergy) increase the postoperative mortality rate in rats in which obstructive...
Article
Different systemic and local responses to mycobacterial antigens suggest an active compartmentalization of responsive lymphocytes to tubercular antigens. This fact, observed in pleuritic processes, raises doubts about the accuracy of information obtained in the study of cells taken solely from peripheral blood. For this reason we decided to study t...
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Purpose: To evaluate the utility, for diagnosis of tuberculous pleurisy, of determining neopterin and lysozyme in pleural fluid rather than adenosine deaminase (ADA), a marker of proven diagnostic efficiency. Methods: Application of pre-established criteria to effusions from 88 patients diagnosed 21 transudates [from 13 men (m) and 8 women (w) aged...
Article
N-1- or 2-anthraquinonyl derivatives of p-methyl-, p-methoxy- and p-aminobenzenesulfonamides were synthesized and evaluated in vitro as antitumour agents. Only N-(1-anthraquinonyl)-p-methylbenzenesulfonamide (1) and -p-methoxy benzene sulfonamide derivatives 3 and 4 showed slight antitumour activity.
Article
In this work, we demonstrate that the simultaneous injection of PC-KLH and Neisseria meningitidis-derived antigens [NMB or PC-(NMB)HI] induced in old NZB/W mice defective responses as does PC-KLH challenge. On the other hand, the simultaneous injection of both immunogenic preparations of N. meningitidis evoked responses similar to those shown by ol...
Article
Standard oil and various non-oily adjuvants were compared for use in immunization against the Aujeszky's disease (pseudorabies) virus, both in mice and swine, and using either inactivated virions or purified glycoproteins as antigen. Mineral oil, sodium alginate, aluminium hydroxide, and saponin were assayed in mice as adjuvants for inactivated vir...
Article
The immunogenic properties of a series of glycoprotein preparations are compared using inactivated conventional vaccines as reference. Serological response and protective efficacy of vaccination of mice and pigs are evaluated for glycoprotein immunogens obtained from various sources. BHK-21 cell cultures were infected with Aujeszky's disease virus...
Article
The polypeptide and glycopolypeptide composition of a local virulent Aujeszky's disease virus (suid herpesvirus 1, SHV-1) strain (E-974) was determined in order to characterize the individual SHV-1 antigens inducing the serological responses in immunized and non-immunized animals. A commercially available inactivated vaccine of known efficacy and t...
Article
The polypeptide and glycopolypeptide composition of a local virulent Aujeszky's disease virus (suid herpesvirus 1, SHV-1) strain (E-974) was determined in order to characterize the individual SHV-1 antigens inducing the serological responses in immunized and non-immunized animals. A commercially available inactivated vaccine of known efficacy and t...
Thesis
Hemos estudiado la cinetica de sintesis de las proteinas de infeccion de una cepa local (e 974) del virus shv-1 (herpesvirus porcino) comparandola con una cepa virulenta (phylaxia) y una cepa atenuada (bartha), sobre dos lineas celulares de distinta estirpe, celulas pk-15 (estirpe epitelial) y celulas bhk-21 (estirpe fibroblastica). hemos observado...
Article
Antibody titers and avidity of sera of autoimmune NZB/W mice in responses induced by different antigens were determined. Results show an age-dependent decrease of the antibody titer in sera from female mice immunized with phosphorylcholine coupled to keyhole limpet haemocyanin. This decrease was not detected when using as immunogen an antigenic pre...
Article
Full-text available
We have tested the ability of the nematode Trichinella to infect young and old (NZB x NZW) F1 (BWF1) mice. We report the capacity of these mice to respond to the parasite antigens containing the epitope phosphorylcholine. The values obtained in adult worm intestinal retention rates and in the number of encysted larvae on the 35th day postinfection...
Article
The immune response to phosphorylcholine (PC) antigens has been extensively studied in recent years. Neisseria meningitidis serogroup B M986 (NMB) was recently reported to induce a PC-specific plaque-forming cell (PFC) immuno-response in mice, a characteristic useful for the study of immunomodulating properties of N. meningitidis. With this techniq...
Article
Neisseria meningitidis group B strain M986 (serotypes 2a, 7) (NMB) elicits a specific primary antiphosphorylcholine immune response in mice but not a secondary response. The ability of other serotype and serogroup meningococci to induce similar primary responses in mice was studied, as was the immunogenicity of trinitrophenyl coupled NMB (TNP-NMB)...
Article
The idiotypic profile of anti-phosphorylcholine plaque-forming cell responses and their evolution with ageing were studied in (NZB X NZW) F1 mice. Our results showed that the anti-phosphorylcholine plaque-forming cell response induced by phosphorylcholine coupled to keyhole limpet haemocyanin and, paralleling, the T15 idiotype clonal dominance decl...
Article
Full-text available
Results of our previous work have shown that Neisseria meningitidis serogroup B M986 can induce a phosphorylcholine (PC)-specific plaque-forming cell immunoresponse in mice. Also, a single injection of a relatively low dose of meningococci in NBF1 female mice induced a priming time-dependent suppression on subsequent meningococcus challenge. This s...
Article
Autoimmune (NZB x NZW)FI (BWFI) mice show an age-dependent, progressive decline in their responsiveness to phosphorylcholine coupled to keyhole limpet haemocyanin (PC-KLH), detected as phosphorylcholine (PC) specific plaque forming cell (PFC) responses; this is paralleled by a decrease in the T15 dominant idiotype expression. This loss of immune co...
Article
The (NZB x NZW) FI (BWFl) mice develop an age dependent autoirnrnune syndrome characterized by severa! immune alterations, such as the loss of immune competence against several thymus-dependent (TD) or thyrnus-independent (TI) antigens. In aged BWF1 mice, the loss of immune competence against the TD antigen phosphorylcholine coupled to keyhole limp...
Article
Full-text available
Specific antiphosphorylcholine immune responses were found to be elicited by different Neisseria meningitidis group B M986 preparations. Our results suggest the functional presence of phosphorylcholine in the bacteria. The immune responses, mostly immunoglobulin M, were measured with a plaque-forming cell assay. The secondary phosphorylcholine-spec...
Article
Partition in two-polymer aqueous phases is a well known, highly sensitive and versatile rnethod for the separation and subfractionation of cell populations based on their surface properties. Factors affecting partition of cells between two immiscible phases, such as membrane charge, hydrophobicity, specific surface receptors and other surface prope...
Article
In order to evaluate the utility of enzyme galleries in the differential diagnosis of human lymphoid malignancies, we have determined ninety-three cytosolic enzymic activities in four human lymphomas (Hodgkin's disease, nodular esclerosing type; immunoblastic sarcoma, B-cell type; plasmacytoid lymphocytic lymphoma and prolymphocytoid transformation...
Article
Full-text available
Clofibrate (CPIB) is a drug applied as an antilipidaemic agent in mammals. In this work we have tested its efficacy in vivo on the growth kinetics of P 1798(sc) lymphoma transplanted to recipient (BALB/c x AKR)F1 mice. Our results show a facilitation of the tumour growth rate in treated recipients. This fact may be related to an effect of the agent...
Article
Ninety-three cytosolic enzymatic activities in normal and tumoural murine tissues were tested using a semi-quantitative colorimetric method. Three experimental approaches were devised: (1) comparison of normal tissues versus tumour tissues, (2) determination of enzyrnatic profiles in three distinct murine tumours, and (3) analysis of differences in...
Article
Expression of tumoral antigens and their masking by sialic acids were studied. Tumour with greater content in sialic acid [P1798(sc)] can surpass the histocompatibility barrier. Furthermore, removal of sialic acid residues from membrane by neuramidase treatment results in a retarded or inhibited tumoral growth. These results suggest an antigenic ma...

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