Rafael R Robaina

Rafael R Robaina
Universidad de Las Palmas de Gran Canaria | ULPGC · Department of Biology

Professor

About

87
Publications
22,290
Reads
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1,014
Citations
Citations since 2017
14 Research Items
405 Citations
2017201820192020202120222023020406080
2017201820192020202120222023020406080
2017201820192020202120222023020406080
2017201820192020202120222023020406080
Additional affiliations
September 1995 - September 1995
Bulgarian Academy of Sciences
Position
  • Researcher
January 1993 - present
Universidad de Las Palmas de Gran Canaria
Position
  • Professor (Full)

Publications

Publications (87)
Article
Full-text available
The synthesis of cell-wall sulfated galactans proceeds through UDP galactose, a major nucleotide sugar in red seaweed, whilst sulfate is transported through S-transporters into algae. Moreover, synthesis of ethylene, a volatile plant growth regulator that plays an important role in red seaweed reproduction, occurs through S-adenosyl methionine. Thi...
Article
Full-text available
Carrageenan, the foremost constituent of extracellular matrix of some rhodophyta, is a galactan backbone with a different number of sulphate groups attached. Variations of degree of sulphation are associated with different types of carrageenans, which vary according to seaweed life cycles, and have consequences for the exploitation of this raw mate...
Article
Full-text available
Grateloupia imbricata is an intertidal marine seaweed and candidate model organism for both industry and academic research, owing to its ability to produce raw materials such as carrageenan. Here we report on the transcriptome of G. imbricata with the aim of providing new insights into the metabolic pathways and other functional pathways related to...
Article
Ethylene favors carposporogenesis in the red seaweed Grateloupia imbricata. Analyses of cystocarp development in vitro in thalli treated with ethylene suggest the interconnection between polyamine and ethylene biosynthesis pathways. Yet little is known about molecular mechanisms underlying carposporogenesis. Here we used droplet digital PCR to anal...
Article
Gelidium corneum is a species of red algae notable for its commercial important as an agarophyte in Morocco. Several regions from the Moroccan Atlantic show that this alga is an endangered species due to the excessive tearing. Hence, the repopulation of these areas is necessary. The in vitro culture of the species was carried out in three media: en...
Article
The extremely delicate situation affecting the seagrass Zostera noltii in the Canary Islands (listed as ‘threatened with extinction') requires urgent measures to ensure its preservation. The genetic or genotypic description of populations and knowledge about their connectivity are important genetic issues that should be taken into account to accomp...
Chapter
Reports on the production of volatile compounds in algae have been focused on what they produce rather than on their functions. In this scenario, a myriad of fatty acid derivatives, nitrogen-containing compounds, organic halogen compounds, sulphur compounds and compounds derived from transferase activity have been described. Recently, a broad range...
Article
Full-text available
When applied in vitro, methyl jasmonate is sensed by the red seaweed Grateloupia imbricate, substantially and visually affecting its carposporogenesis. However, although there is some understanding of the morphological changes induced by methyl jasmonate in vitro, little is known about the genes that are involved in red seaweed carposporogenesis an...
Article
Full-text available
Macroalgae (seaweeds) are the subject of increasing interest for their potential as a source of valuable, sustainable biomass in the food, feed, chemical and pharmaceutical industries. Compared with microalgae, the pace of knowledge acquisition in seaweeds is slower despite the availability of whole-genome sequences and model organisms for the majo...
Article
Full-text available
Zarranz Elso, M.; Manent, P., and Robaina, R.R., 2018. Zostera noltii in the Canary Islands: A genetic description for conservation purposes. The extremely delicate situation affecting the seagrass Zostera noltii in the Canary Islands (listed as 'threatened with extinction') requires urgent measures to ensure its preservation. The genetic or genoty...
Article
Seagrass meadows provide an essential ecological service in coastal ecosystems worldwide, although they are sensitive to many human factors, as a serious global regression has been documented. During sampling along the Atlantic Moroccan coast, five coastal lagoons were found characterized by the presence of intertidal monospecific seagrass Zostera...
Article
Full-text available
Here we highlight the effects of methyl jasmonate (MeJa) on cystocarp development in the red macroscopic alga Grateloupia imbricata Holmes. In G. imbricata, jasmonate release is related to the reproductive state, as fertile thalli (i.e., those that have cystocarps) released significant amounts of this volatile compound (1.27 ± 0.20 mM (•) mg fw(-1)...
Article
Full-text available
To gain a better understanding of the regulatory mechanism(s) modulating expression of the ornithine decarboxylase gene ODC during cystocarp development in the red seaweed Grateloupia imbricata, DNA motifs found in the 5�-upstream region of the gene were identified by in silico analysis. In addition, when infertile G. imbricata thalli were treated...
Article
To gain a better understanding of the regulatory mechanism(s) modulating expression of the ornithine decarboxylase gene ODC during cystocarp development in the red seaweed Grateloupia imbricata, DNA motifs found in the 5'-upstream region of the gene were identified by in silico analysis. In addition, when infertile G. imbricata thalli were treated...
Article
Full-text available
Genetic description and remote sensing techniques as management tools for Zostera noltii seagrass populations along the Atlantic Moroccan coast. Seagrass meadows provide an essential ecological service in coastal ecosystems worldwide, although they are sensitive to many human factors, as a serious global regression has been documented. During sampl...
Article
Full-text available
Multicellular red algae (Rhodophyta) have some of the most complex life cycles known in living organisms. Economically valuable seaweeds, such as phycocolloid producers, have a triphasic (gametophyte, carposporophyte, and tetrasporophyte) life cycle, not to mention the intricate alternation of generations in the edible "sushi-alga" nori. It is a we...
Article
Full-text available
The endogenous polyamine (Pas) content of free and bound-acid soluble fractions was assessed in the epiphyte Bonnemaisonia hamifera under the presence of its host Gelidium arbuscula and of extracts obtained from G. arbuscula and from G. robustum. In the presence of fresh thalli of G. arbuscula the free putrescine (PUT) content decreased (P ‹ 0.05),...
Conference Paper
Full-text available
Seagrass meadows provide an essential ecological service in coastal ecosystems worldwide, although they are sensitive to many environmental and human factors having been documented a serious global regression. Along the Atlantic Moroccan Coast we found 5 areas with maximum environmental interest as coastal lagoons characterized by the presence of i...
Article
The effects of different light conditions and exogenous ethylene on the emission of volatile compounds from the alga Gelidium arbuscula Bory de Saint‐Vincent were studied. Special emphasis was placed on the possibility that the emission of ethylene and dimethyl sulfide (DMS) are related through the action of dimethylsulfoniopropionate (DMSP) lyase....
Book
Full-text available
Cymodocea nodosa es una fanerógama marina que forma praderas submarinas en fondos arenosos y bien iluminados del litoral canario. Estas praderas son ecosistemas muy importantes desde el punto de vista ecológico y económico, ya que sirven de refugio a un gran número de especies de interés comercial. Sin embargo, la fuerte presión antrópica a la que...
Article
Full-text available
In vitro germinated seedlings of Cymodocea nodosa were used as a source of young embryonic tissue for cell culture. Histological study of the cotyledon revealed the presence of meristematic tissue that gave rise to the first leaf of the plantlet, which released large numbers of cells after enzymatic digestion and thus indicated great growth potenti...
Conference Paper
Full-text available
TechMARAT is a cross-border cooperation project between the Atlantic Cooperation Area (Canary Islands) and the region of Sous Massa-Draa (Morocco). The main objective of the project is the promotion of relationships between the border regions of the Canary Islands and the Souss Massa Draa Region in Morocco, through the exchange of technician and te...
Article
Full-text available
Endogenous polyamine (PA) content in the seagrass Cymodocea nodosa was monitored at different embryo and seedling developmental stages. Total PA concentration, including putrescine (Put), spermidine (Spd) and spermine (Spm), and the Put (Spm + Spd)−1 ratio decreased during embryo development, from the immature fruit to the mature seed stage (with a...
Article
ABSTRACT: Endogenous polyamine (PA) content in the seagrass Cymodocea nodosa was monitored at different embryo and seedling developmental stages. Total PA concentration, including putrescine (Put), spermidine (Spd) and spermine (Spm), and the Put (Spm + Spd)−1 ratio decreased during embryo development, from the immature fruit to the mature seed sta...
Article
Full-text available
Carpospore output and development in the marine red alga Hydropuntia cornea J. Agardh. were increased by adding polyamines (PAs) (putrescine, spermidine and spermine) singly or in combinations at 10(-9), 10(-6) and 10(-3) M. Cell divisions after spore release and development of apical axis between 17 and 21 days characterized carpospore development...
Article
The effects of ethylene (C2H4) on tetrasporogenesis of the red seaweed Pterocladiella capillacea (S. G. Gmelin) Bornet were investigated. Ethylene is a gaseous hormone that is involved in a variety of physiological processes (e.g., flowering, fruit abscission) in higher plants. To study the effects of ethylene on the reproduction of the red seaweed...
Article
Full-text available
The red alga Gratelupia doryphora was cultivated in Provasoli enricher (plain) seawater and in a glycerol media. The incubation in seawater leads to an increase in the percentage of polyunsaturated acids, while the glycerol increase the total lipid content. If the alga is being grown as a heterotrophic culture, it is possible to increase the alga b...
Article
We evaluated seed storage and effects of plant growth regulators and fertilizers on in vitro germinated seedlings with a view to optimizing restoration of Cymodocea nodosa. Storage of seeds in seawater at room temperature (22"28C) and 188C retarded germination potential for up to one year, though the best results were seen within the first six mont...
Article
Full-text available
Cymodocea nodosa, a marine angiosperm, an ecosystem engineer in the Mediterranean Sea and the Northwest Atlantic Ocean; however, as in other seagrasses meadows worldwide, the swards are actually declining due to increasing human pressures. Hence, we have developed an effective propagation methodology that provides C. nodosa seedlings for seagrass m...
Article
This paper describes the cloning of the ornithine decarboxylase gene from a red seaweed, Grateloupia imbricata (Rhodophyta), the characterization of its expression throughout the reproductive process, and demonstrates how polyamines are involved in seaweed reproduction. In addition, the data indicate that the basal perennial and non-spore-forming t...
Article
Full-text available
Advances have been made in cell and tissue culture of seaweeds to define a unique branch of in vitro techniques; however, they are lagging far behind those of land plants and have limited applications. Explants can be cultivated axenically in enriched or artificial seawater culture media, and regeneration and even callus formation are achieved. In...
Article
Specimens of Grateloupia from Gran Canaria in the Canary Islands were used to molecularly ascertain which of the species has been used in physiological and bio-technological experiments. The rbcL sequence analysis revealed that four out of five analyzed specimens (i.e. those commonly collected for physiological research) formed a monophyletic clade...
Article
Full-text available
Polyamines such as putrescine, spermidine and spermine are ubiquitous aliphatic amines involved in reproductive events in plants and algae, and first become evident through changes in endogenous levels during reproductive development. To examine whether the differences observed in polyamines, during carposporogenesis, in the red alga Grateloupia, f...
Article
Polyamines (PAs), such as diamine putrescine (PUT), triamine spermidine (SPD) and tetraamine spermine (SPM) have been related to environmental stress, including salt stress. A marine red macrophyte alga Grateloupia doryphora (Montagne) Howe was used to investigate the role of PAs during acclimation to moderate hyposaline conditions (incubation 24h...
Article
In vitro methods of plant micro-propagation are being considered as a possible solution for the decline in seagrass communities registered worldwide. To achieve successful plant micro-propagation, culture conditions are commonly adjusted empirically within almost species-specific conditions, to comply to the following three conditions: (i) culture...
Article
In vitro methods of plant micro-propagation are being considered as a possible solution for the decline in seagrass communities registered worldwide. To achieve successful plant micro-propagation, culture conditions are commonly adjusted empirically within almost species-specific conditions, to comply to the following three conditions: (i) culture...
Chapter
Full-text available
La materia "Introducción a las Ciencias de la Naturaleza" es una asignatura de libre elección para los alumnos del Curso Preparatorio para Mayores de 25 años que organiza la Universidad de Las Palmas de Gran Canaria. Sus contenidos han sido escogidos y preparados por un grupo de profesores doctores del Departamento de Biología de la Universidad, pa...
Article
Polyamines (PAs) such as putrescine (PUT), spermidine (SPD), and spermine (SPM) are ubiquitous aliphatic amines involved in widely varying physiological behavior, but particularly they are actively involved in cell growth, division, and differentiation during reproductive events in plants. The contents of PUT, SPD, and SPM in infertile and fertile...
Article
The fatty acid composition of the lipids from the sponge Hymeniacidon sanguinea was investigated and 73 acids were identified. Three of them were new and their structures, elucidated by gas chromatography/mass spectrometry, were identified as 13-methyl-icosanoic, 15-methyl-docosanoic and 3,13-dimethyl-tetradecanoic. Only 12 sterols were present, wi...
Article
Full-text available
Polyamines (PAs) such as putrescine (PUT), spermidine (SPD), and spermine (SPM) are ubiquitous aliphatic amines involved in widely varying physiological behavior, but particularly they are actively involved in cell growth, division, and differentiation during reproductive events in plants. The contents of PUT, SPD, and SPM in infertile and fertile...
Article
Full-text available
Naturally occurring levels of putrescine, spermidine, and spermine were analyzed in female gametophyte (nonfertilized) and reproductive tissues (cystocarps) at two different stages of development in the marine red algae Gracilaria cornea J. Agardh. Endogenous polyamine levels changed at differential stages of cystocarp maturation. Highest polyamine...
Article
The fatty acid composition of the lipids from the sponge Verongia aerophoba was investigated and 60 acids were identified. Two of them were new and their structures were elucidated by gas chromatography-mass spectrometry. These acids were identified as 20-methylhexacosanoic and Δ5, 9, 22-nonacosatrienoic. Only 13 sterols were present, and aplystero...
Article
Full-text available
The fatty acid composition of the lipids from Chondrosia reniformis (Nardo, 1847) was investigated and 57 acids were identified. One of them is new for nature and its structure was elucidated by GC/mass-spectrometry. This acid was identified as 8,10-dimethyl-16:0. The sterol composition was relatively simple and only 12 sterols were present. In the...
Article
The levels of putrescine (Put), spermidine (Spd) and spermine (Spm) were analyzed in naturally collected samples of the marine macroalgae Dyctiota dichotoma, Gelidium canariensis and Grateloupia doryphora. Polyamines (PAs) appeared in free (35–134 μg g−1 fresh weight) and bound TCA-insoluble form (1 667–2 624 μg g−1 fresh weight). Axenic in vitro c...
Article
The levels of the polyamines putrescine, spermidine and spermine were analysed in different tissues of nature-collected samples of the seagrass Cymodocea nodosa. The highest accumulation of polyamines was found in the apical section of the rhizome. The putrescine/spermine ratio was highest in the senescent tissue of the leaf.
Article
A sporulation and sterilization procedure was used to establish axenic cultures of sporelings of Gelidium canariensis. Sporangial branchlets excised from the thallus were rinsed in distilled water twice and in 1% sodium hypochlorite (2 min). The branchlets were cultivated overnight in multiwell plates with 0.3 ml of autoclaved seawater to promote s...
Technical Report
RECOMENDACIONES - Los procesos de colmatacion con arena y de salinizaci6n de la laguna aconsejan el proceder a su dragado cuanto antes, particularmente teniendo en consideración que Ios obras de restauracion de la playa ya se han ejecutado. - El dragado se debe realizar unicamente en la orilla Oeste sin que afecte al resto de la laguna, que en ning...
Article
Full-text available
With 6 figures ABSTRACT. An annual evaluation (June 1994-July 1995) of the water quality from Punta de Tenefé to Punta de Taozo (Gran Canaria, Canary Islands) was done, as a part of an environmental assessment program in tourist coast. The ecological parameters to evaluate were chosen as representative of the hurnan pressure exerted on the coast (c...
Article
Full-text available
The influence of the plant growth regulators 2,4-D, GA3, BA and kinetin, and the polyamines putrescine, spermidine and spermine were tested on axenic in vitro cultures of carposporelings of Grateloupia doryphora. The auxin 2,4-D (10-3 M) and the polyamine spermine (10-6 M and 10-3 M) induced a callus (disorganised cell mass that arose from the orga...
Article
More experimental evidence is needed to understand the role of propagules in macroalgal biology. There are no reports in the literature on the comparative physiology (e.g. photosynthesis) of sporelings and adults. In this paper we report on the␣variation␣in␣photosynthetic parameters (maximum photosynthesis, P max , and efficiency, alpha, and dark...
Article
Full-text available
The red alga Grateloupia doryphora (Montagne) Howe was grown in axenic culture in Provasoli's enriched seawater medium supplemented with glycerol as an organic carbon source. Carpospore seedlings showed a particular form of cell proliferation forming compact cell masses with many buds or protrusions after 12 days of growth. Histologic and histochem...
Conference Paper
An annual evaluation (June 1994-July 1995) of the water quality from Punta de Tenefé to Punta de Taozo (Gran Canaria, Canary Islands) was done, as a part of an environmental assessment program in tourist coast. The ecological parameters to evaluate were chosen as representative of the hurnan pressure exerted on the coast (crowded beaches, direct wa...
Conference Paper
Full-text available
An annual evaluation (June 1994-July 1995) of the water quality from Punta de Tenefé to Punta de Taozo (Gran Canaria, Canary Islands) was done, as a part of an environmental assessment program in tourist coast. The ecological parameters to evaluate were chosen as representative of the hurnan pressure exerted on the coast (crowded beaches, direct wa...
Article
The red alga Grateloupia doryphora (Montagne) Howe was grown in axenic culture in Provasoli's enriched seawater medium supplemented with glycerol as an organic carbon source. Carpospore seedlings showed a particular form of cell proliferation forming compact cell masses with many buds or protrusions after 12 days of growth. Histologic and histochem...
Article
The implications of light in structural changes during growth and development of a carpospore seedling from Grateloupia doryphora cultivated in glycerol were studied by changing photon flux density from the standard white light of 30 μmol photons m-2s-1 to 20, 60 and 100 μmol photons m-2s-1 and spectra from white to both blue and red light (30 μmol...
Article
Full-text available
Heterotrophic activity in macroalgae has been little studied, but the red macroalga Grateloupia doryphora is known to grow in light at a higher rate in a glycerol-containing medium than in seawater. The effects of 0·1 M exogenous glycerol in seawater (SW90-gly) on the respiration rate of G. doryphora and the role played by light were investigated....
Article
The occurrence of several forms of disorganized growth (filamentous, groups of cells and compact cell masses) has been reported in seaweed tissue culture as callus or callus-like, and in nature as galls, tumours or callus. Laurencia sp. formed callus in culture. To know whether callus or callus-like types of growth also occurred during regeneration...
Article
Full-text available
Explants of Gelidium versicolor, Grateloupia doryphora and Laurencia sp. were cultivated in Provasoli enriched seawater culture medium (PES) adjusted to several osmolalities (0.5, 0.7, 1.0 and 1.5 Os kg–1) and solidities (agar concentration = 3, 8 and 15 g L–1). Osmolality was adjusted by dilution of seawater with distilled water (50, 70 and 100% s...
Article
Full-text available
Explants of Grateloupia doryphora were cultivated in Provasoli Enriched Seawater culture medium (PES) supplemented with glycerol (0.1, 0.3, 0.5 or 0.8 mol 1-1) or carbohydrates (0.1 or 0.3 mol 1-1 mannose, glucose and galactose) and agar (3, 8, 15 g 1-1 ). The osmolality of the medium was adjusted by dilution of the seawater (70 or 100%, v/v). The...
Chapter
Tissue culture techniques could be applied to improve the genetic qualities of seaweeds. In order to apply those techniques, efficient methods for obtaining and regenerating calli are needed. The term “callus” or “calluslike” has been applied to define very different structures in seaweeds (Dixon 1963; Fries 1980; Chen 1982; Saga et al. 1982; Tseko...
Chapter
The genetic basis for NaCl tolerance in the tomato has been proved (Rush and Epstein 1976, 1981). Salt tolerance mechanisms are thought to be cell-based and do not depend on whole plant organization in Lycopersicon (Tal et al. 1978, Tal and Katz 1980, Taleinsk-Gertel et al. 1983). Different mechanisms for NaCl tolerance have been described: salt ex...

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