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January 1993 - present
Publications
Publications (93)
Humans have relied on oceans since time immemorial and have exploited them for natural resources and recreational activities. With the advancement of technologies, rapid population growth and land-use change, the dependability on marine ecosystems has increased tremendously. Anthropogenic activities are causing increased global temperatures, altere...
Floridoside is a galactosyl–glycerol compound that acts to supply UDP-galactose and functions as an organic osmolyte in response to salinity in Rhodophyta. Significantly, the UDP-galactose pool is shared for sulfated cell wall galactan synthesis, and, in turn, affected by thallus development alongside carposporogenesis induced by volatile growth re...
In this study, we monitored changes in cell wall sulfated galactans in the red seaweed Grateloupia imbricata after the rapid (48 h) induction of cystocarp maturation and carpospore development by the volatile hormone methyl jasmonate (MEJA). Synthesis of sulfated galactans, carrageenans, which requires sulfated UDP galactose, was followed by expres...
Zarranz Elso, M.; Manent, P.; Luque, A.; Ramdani, M., and Robaina, R.R., 2017. Genetic description and remote sensing techniques as management tools for Zostera noltii seagrass populations along the Atlantic Moroccan coast. Journal of Coastal Research, 33(1), 78-87. Coconut Creek (Florida), ISSN 0749-0208. Seagrass meadows provide an essential ecol...
The synthesis of cell-wall sulfated galactans proceeds through UDP galactose, a major nucleotide sugar in red seaweed, whilst sulfate is transported through S-transporters into algae. Moreover, synthesis of ethylene, a volatile plant growth regulator that plays an important role in red seaweed reproduction, occurs through S-adenosyl methionine. Thi...
Carrageenan, the foremost constituent of extracellular matrix of some rhodophyta, is a galactan backbone with a different number of sulphate groups attached. Variations of degree of sulphation are associated with different types of carrageenans, which vary according to seaweed life cycles, and have consequences for the exploitation of this raw mate...
Saluda del volumen 28, número conmemortivo de los 30 años de la ULPGC
Grateloupia imbricata is an intertidal marine seaweed and candidate model organism for both industry and academic research, owing to its ability to produce raw materials such as carrageenan. Here we report on the transcriptome of G. imbricata with the aim of providing new insights into the metabolic pathways and other functional pathways related to...
Ethylene favors carposporogenesis in the red seaweed Grateloupia imbricata. Analyses of cystocarp development in vitro in thalli treated with ethylene suggest the interconnection between polyamine and ethylene biosynthesis pathways. Yet little is known about molecular mechanisms underlying carposporogenesis. Here we used droplet digital PCR to anal...
Gelidium corneum is a species of red algae notable for its commercial important as an agarophyte in Morocco. Several regions from the Moroccan Atlantic show that this alga is an endangered species due to the excessive tearing. Hence, the repopulation of these areas is necessary. The in vitro culture of the species was carried out in three media: en...
The extremely delicate situation affecting the seagrass Zostera noltii in the Canary Islands (listed as ‘threatened with extinction') requires urgent measures to ensure its preservation. The genetic or genotypic description of populations and knowledge about their connectivity are important genetic issues that should be taken into account to accomp...
Reports on the production of volatile compounds in algae have been focused on what they produce rather than on their functions. In this scenario, a myriad of fatty acid derivatives, nitrogen-containing compounds, organic halogen compounds, sulphur compounds and compounds derived from transferase activity have been described. Recently, a broad range...
When applied in vitro, methyl jasmonate is sensed by the red seaweed Grateloupia imbricate, substantially and visually affecting its carposporogenesis. However, although there is some understanding of the morphological changes induced by methyl jasmonate in vitro, little is known about the genes that are involved in red seaweed carposporogenesis an...
Macroalgae (seaweeds) are the subject of increasing interest for their potential as a source of valuable, sustainable biomass in the food, feed, chemical and pharmaceutical industries. Compared with microalgae, the pace of knowledge acquisition in seaweeds is slower despite the availability of whole-genome sequences and model organisms for the majo...
Zarranz Elso, M.; Manent, P., and Robaina, R.R., 2018. Zostera noltii in the Canary Islands: A genetic description for conservation purposes. The extremely delicate situation affecting the seagrass Zostera noltii in the Canary Islands (listed as 'threatened with extinction') requires urgent measures to ensure its preservation. The genetic or genoty...
Seagrass meadows provide an essential ecological service in coastal ecosystems worldwide, although they are sensitive to many human factors, as a serious global regression has been documented. During sampling along the Atlantic Moroccan coast, five coastal lagoons were found characterized by the presence of intertidal monospecific seagrass Zostera...
Here we highlight the effects of methyl jasmonate (MeJa) on cystocarp development in the red macroscopic alga Grateloupia imbricata Holmes. In G. imbricata, jasmonate release is related to the reproductive state, as fertile thalli (i.e., those that have cystocarps) released significant amounts of this volatile compound (1.27 ± 0.20 mM (•) mg fw(-1)...
To gain a better understanding of the regulatory mechanism(s) modulating expression of the ornithine
decarboxylase gene ODC during cystocarp development in the red seaweed Grateloupia imbricata, DNA
motifs found in the 5�-upstream region of the gene were identified by in silico analysis. In addition,
when infertile G. imbricata thalli were treated...
To gain a better understanding of the regulatory mechanism(s) modulating expression of the ornithine decarboxylase gene ODC during cystocarp development in the red seaweed Grateloupia imbricata, DNA motifs found in the 5'-upstream region of the gene were identified by in silico analysis. In addition, when infertile G. imbricata thalli were treated...
Genetic description and remote sensing techniques as management tools for Zostera noltii seagrass populations along the Atlantic Moroccan coast. Seagrass meadows provide an essential ecological service in coastal ecosystems worldwide, although they are sensitive to many human factors, as a serious global regression has been documented. During sampl...
Multicellular red algae (Rhodophyta) have some of the most complex life cycles known in living organisms. Economically valuable seaweeds, such as phycocolloid producers, have a triphasic (gametophyte, carposporophyte, and tetrasporophyte) life cycle, not to mention the intricate alternation of generations in the edible “sushi-alga” nori. It is a we...
The endogenous polyamine (Pas) content of free and bound-acid soluble fractions was assessed in the epiphyte Bonnemaisonia hamifera under the presence of its host Gelidium arbuscula and of extracts obtained from G. arbuscula and from G. robustum. In the presence of fresh thalli of G. arbuscula the free putrescine (PUT) content decreased (P ‹ 0.05),...
Seagrass meadows provide an essential ecological service in coastal ecosystems worldwide, although they are sensitive to many environmental and human factors having been documented a serious global regression. Along the Atlantic Moroccan Coast we found 5 areas with maximum environmental interest as coastal lagoons characterized by the presence of i...
The effects of different light conditions and exogenous ethylene on the emission of volatile compounds from the alga Gelidium arbuscula Bory de Saint‐Vincent were studied. Special emphasis was placed on the possibility that the emission of ethylene and dimethyl sulfide (DMS) are related through the action of dimethylsulfoniopropionate (DMSP) lyase....
Cymodocea nodosa es una fanerógama marina que forma praderas submarinas en fondos arenosos y bien iluminados del litoral canario. Estas praderas son ecosistemas muy importantes desde el punto de vista ecológico y económico, ya que sirven de refugio a un gran número de especies de interés comercial. Sin embargo, la fuerte presión antrópica a la que...
In vitro germinated seedlings of Cymodocea
nodosa were used as a source of young embryonic tissue
for cell culture. Histological study of the cotyledon
revealed the presence of meristematic tissue that gave
rise to the first leaf of the plantlet, which released large
numbers of cells after enzymatic digestion and thus
indicated great growth potenti...
TechMARAT is a cross-border cooperation project between the Atlantic Cooperation Area (Canary Islands) and the region of Sous Massa-Draa (Morocco). The main objective of the project is the promotion of relationships between the border regions of the Canary Islands and the Souss Massa Draa Region in Morocco, through the exchange of technician and te...
Endogenous polyamine (PA) content in the seagrass Cymodocea nodosa was monitored
at different embryo and seedling developmental stages. Total PA concentration, including
putrescine (Put), spermidine (Spd) and spermine (Spm), and the Put (Spm + Spd)−1 ratio decreased
during embryo development, from the immature fruit to the mature seed stage (with a...
ABSTRACT: Endogenous polyamine (PA) content in the seagrass Cymodocea nodosa was monitored
at different embryo and seedling developmental stages. Total PA concentration, including
putrescine (Put), spermidine (Spd) and spermine (Spm), and the Put (Spm + Spd)−1 ratio decreased
during embryo development, from the immature fruit to the mature seed sta...
Carpospore output and development in the marine red alga Hydropuntia cornea J. Agardh. were increased by adding polyamines (PAs) (putrescine, spermidine and spermine) singly or in combinations at 10(-9), 10(-6) and 10(-3) M. Cell divisions after spore release and development of apical axis between 17 and 21 days characterized carpospore development...
The effects of ethylene (C2H4) on tetrasporogenesis of the red seaweed Pterocladiella capillacea (S. G. Gmelin) Bornet were investigated. Ethylene is a gaseous hormone that is involved in a variety of physiological processes (e.g., flowering, fruit abscission) in higher plants. To study the effects of ethylene on the reproduction of the red seaweed...
The red alga Gratelupia doryphora was cultivated in Provasoli enricher (plain) seawater and in a glycerol media. The incubation in seawater leads to an increase in the percentage of polyunsaturated acids, while the glycerol increase the total lipid content. If the alga is being grown as a heterotrophic culture, it is possible to increase the alga b...
We evaluated seed storage and effects of plant growth regulators
and fertilizers on in vitro germinated seedlings with
a view to optimizing restoration of Cymodocea nodosa. Storage
of seeds in seawater at room temperature (22"28C) and
188C retarded germination potential for up to one year,
though the best results were seen within the first six mont...
Cymodocea nodosa, a marine angiosperm, an ecosystem engineer in the Mediterranean Sea and the Northwest Atlantic Ocean; however, as in other seagrasses meadows worldwide, the swards are actually declining due to increasing human pressures. Hence, we have developed an effective propagation methodology that provides C. nodosa seedlings for seagrass m...
This paper describes the cloning of the ornithine decarboxylase gene from a red seaweed, Grateloupia imbricata (Rhodophyta), the characterization of its expression throughout the reproductive process, and demonstrates how polyamines are involved in seaweed reproduction. In addition, the data indicate that the basal perennial and non-spore-forming t...
Advances have been made in cell and tissue culture of seaweeds to define a unique branch of in vitro techniques; however, they are lagging far behind those of land plants and have limited applications. Explants can be cultivated axenically in enriched or artificial seawater culture media, and regeneration and even callus formation are achieved. In...
Specimens of Grateloupia from Gran Canaria in the Canary Islands were used to molecularly ascertain which of the species has been used in physiological and bio-technological experiments. The rbcL sequence analysis revealed that four out of five analyzed specimens (i.e. those commonly collected for physiological research) formed a monophyletic clade...
Polyamines such as putrescine, spermidine and spermine are ubiquitous aliphatic amines involved in reproductive events in
plants and algae, and first become evident through changes in endogenous levels during reproductive development. To examine
whether the differences observed in polyamines, during carposporogenesis, in the red alga Grateloupia, f...
Polyamines (PAs), such as diamine putrescine (PUT), triamine spermidine (SPD) and tetraamine spermine (SPM) have been related to environmental stress, including salt stress. A marine red macrophyte alga Grateloupia doryphora (Montagne) Howe was used to investigate the role of PAs during acclimation to moderate hyposaline conditions (incubation 24h...
In vitro methods of plant micro-propagation are being considered as a possible solution for the decline in seagrass communities registered worldwide. To achieve successful plant micro-propagation, culture conditions are commonly adjusted empirically within almost species-specific conditions, to comply to the following three conditions: (i) culture...
In vitro methods of plant micro-propagation are being considered as a possible solution for the decline in seagrass communities registered worldwide. To achieve successful plant micro-propagation, culture conditions are commonly adjusted empirically within almost species-specific conditions, to comply to the following three conditions: (i) culture...
La materia "Introducción a las Ciencias de la Naturaleza" es una asignatura de
libre elección para los alumnos del Curso Preparatorio para Mayores de 25 años
que organiza la Universidad de Las Palmas de Gran Canaria. Sus contenidos han
sido escogidos y preparados por un grupo de profesores doctores del
Departamento de Biología de la Universidad, pa...
Polyamines (PAs) such as putrescine (PUT), spermidine (SPD), and spermine (SPM) are ubiquitous aliphatic amines involved in widely varying physiological behavior, but particularly they are actively involved in cell growth, division, and differentiation during reproductive events in plants. The contents of PUT, SPD, and SPM in infertile and fertile...
The fatty acid composition of the lipids from the sponge Hymeniacidon sanguinea was investigated and 73 acids were identified. Three of them were new and their structures, elucidated by gas chromatography/mass spectrometry, were identified as 13-methyl-icosanoic, 15-methyl-docosanoic and 3,13-dimethyl-tetradecanoic. Only 12 sterols were present, wi...
Polyamines (PAs) such as putrescine (PUT), spermidine (SPD), and spermine (SPM) are ubiquitous aliphatic amines involved in widely varying physiological behavior, but particularly they are actively involved in cell growth, division, and differentiation during reproductive events in plants. The contents of PUT, SPD, and SPM in infertile and fertile...
Naturally occurring levels of putrescine, spermidine, and spermine were analyzed in female gametophyte (nonfertilized) and reproductive tissues (cystocarps) at two different stages of development in the marine red algae Gracilaria cornea J. Agardh. Endogenous polyamine levels changed at differential stages of cystocarp maturation. Highest polyamine...
The fatty acid composition of the lipids from the sponge Verongia aerophoba was investigated and 60 acids were identified. Two of them were new and their structures were elucidated by gas chromatography-mass spectrometry. These acids were identified as 20-methylhexacosanoic and Δ5, 9, 22-nonacosatrienoic. Only 13 sterols were present, and aplystero...
The fatty acid composition of the lipids from Chondrosia reniformis (Nardo, 1847) was investigated and 57 acids were identified. One of them is new for nature and its structure was elucidated by GC/mass-spectrometry. This acid was identified as 8,10-dimethyl-16:0. The sterol composition was relatively simple and only 12 sterols were present. In the...
The levels of putrescine (Put), spermidine (Spd) and spermine (Spm) were analyzed in naturally collected samples of the marine macroalgae Dyctiota dichotoma, Gelidium canariensis and Grateloupia doryphora. Polyamines (PAs) appeared in free (35–134 μg g−1 fresh weight) and bound TCA-insoluble form (1 667–2 624 μg g−1 fresh weight). Axenic in vitro c...
The levels of the polyamines putrescine, spermidine and spermine were analysed in different tissues of nature-collected samples of the seagrass Cymodocea nodosa. The highest accumulation of polyamines was found in the apical section of the rhizome. The putrescine/spermine ratio was highest in the senescent tissue of the leaf.
A sporulation and sterilization procedure was used to establish axenic cultures of sporelings of Gelidium canariensis. Sporangial branchlets excised from the thallus were rinsed in distilled water twice and in 1% sodium hypochlorite (2 min). The branchlets were cultivated overnight in multiwell plates with 0.3 ml of autoclaved seawater to promote s...
RECOMENDACIONES
- Los procesos de colmatacion con arena y de salinizaci6n de la laguna aconsejan el proceder a su dragado cuanto antes, particularmente teniendo en consideración que Ios obras de restauracion de la playa ya se han ejecutado.
- El dragado se debe realizar unicamente en la orilla Oeste sin que afecte al resto de la laguna, que en ning...
With 6 figures ABSTRACT. An annual evaluation (June 1994-July 1995) of the water quality from Punta de Tenefé to Punta de Taozo (Gran Canaria, Canary Islands) was done, as a part of an environmental assessment program in tourist coast. The ecological parameters to evaluate were chosen as representative of the hurnan pressure exerted on the coast (c...
The influence of the plant growth regulators 2,4-D, GA3, BA and kinetin, and the polyamines putrescine, spermidine and spermine were tested on axenic in vitro cultures of carposporelings
of Grateloupia doryphora. The auxin 2,4-D (10-3 M) and the polyamine spermine (10-6 M and 10-3 M) induced a callus (disorganised cell mass that arose from the orga...
More experimental evidence is needed to understand the role of propagules in macroalgal biology. There are no reports in
the literature on the comparative physiology (e.g. photosynthesis) of sporelings and adults. In this paper we report on the␣variation␣in␣photosynthetic
parameters (maximum photosynthesis, P
max
, and efficiency, alpha, and dark...
The red alga Grateloupia doryphora (Montagne) Howe was grown in axenic culture in Provasoli's enriched seawater medium supplemented with glycerol as an organic carbon source. Carpospore seedlings showed a particular form of cell proliferation forming compact cell masses with many buds or protrusions after 12 days of growth. Histologic and histochem...
An annual evaluation (June 1994-July 1995) of the water quality from Punta de Tenefé to Punta de Taozo (Gran Canaria, Canary Islands) was done, as a part of an environmental assessment program in tourist coast. The ecological parameters to evaluate were chosen as representative of the hurnan pressure exerted
on the coast (crowded beaches, direct wa...
An annual evaluation (June 1994-July 1995) of the water quality from Punta de Tenefé to Punta de Taozo (Gran Canaria, Canary Islands) was done, as a part of an environmental assessment program in tourist coast. The ecological parameters to evaluate were chosen as representative of the hurnan pressure exerted on the coast (crowded beaches, direct wa...
The red alga Grateloupia doryphora (Montagne) Howe was grown in axenic culture in Provasoli's enriched seawater medium supplemented with glycerol as an organic carbon source. Carpospore seedlings showed a particular form of cell proliferation forming compact cell masses with many buds or protrusions after 12 days of growth. Histologic and histochem...
The implications of light in structural changes during growth and development of a carpospore seedling from Grateloupia doryphora cultivated in glycerol were studied by changing photon flux density from the standard white light of 30 μmol photons m-2s-1 to 20, 60 and 100 μmol photons m-2s-1 and spectra from white to both blue and red light (30 μmol...
Heterotrophic activity in macroalgae has been little studied, but the red macroalga Grateloupia doryphora is known to grow in light at a higher rate in a glycerol-containing medium than in seawater. The effects of 0·1 M exogenous glycerol in seawater (SW90-gly) on the respiration rate of G. doryphora and the role played by light were investigated....
The occurrence of several forms of disorganized growth (filamentous, groups of cells and compact cell masses) has been reported in seaweed tissue culture as callus or callus-like, and in nature as galls, tumours or callus. Laurencia sp. formed callus in culture. To know whether callus or callus-like types of growth also occurred during regeneration...
Explants of Gelidium versicolor, Grateloupia doryphora and Laurencia sp. were cultivated in Provasoli enriched seawater culture medium (PES) adjusted to several osmolalities (0.5, 0.7, 1.0 and 1.5 Os kg–1) and solidities (agar concentration = 3, 8 and 15 g L–1). Osmolality was adjusted by dilution of seawater with distilled water (50, 70 and 100% s...
Explants of Grateloupia doryphora were cultivated in Provasoli Enriched Seawater culture medium (PES) supplemented with glycerol (0.1, 0.3, 0.5 or 0.8 mol 1-1) or carbohydrates (0.1 or 0.3 mol 1-1 mannose, glucose and galactose) and agar (3, 8, 15 g 1-1 ). The osmolality of the medium was adjusted by dilution of the seawater (70 or 100%, v/v). The...
Tissue culture techniques could be applied to improve the genetic qualities of seaweeds. In order to apply those techniques, efficient methods for obtaining and regenerating calli are needed. The term “callus” or “calluslike” has been applied to define very different structures in seaweeds (Dixon 1963; Fries 1980; Chen 1982; Saga et al. 1982; Tseko...
The genetic basis for NaCl tolerance in the tomato has been proved (Rush and Epstein 1976, 1981). Salt tolerance mechanisms are thought to be cell-based and do not depend on whole plant organization in Lycopersicon (Tal et al. 1978, Tal and Katz 1980, Taleinsk-Gertel et al. 1983). Different mechanisms for NaCl tolerance have been described: salt ex...
