Rafael Peláez

Rafael Peláez
Center for Biomedical Research of La Rioja | CIBIR

Bachelor of Biology

About

49
Publications
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610
Citations

Publications

Publications (49)
Article
Full-text available
Age-related macular degeneration is the main cause of irreversible vision in developed countries, and intravitreal anti-vascular endothelial growth factor (anti-VEGF) injections are the current gold standard treatment today. Although anti-VEGF treatment results in important improvements in the course of this disease, there is a considerable number...
Chapter
Finite element method (FEM) biomechanical analyses have proved their effectiveness on prosthesis set up and bone tissue biomechanical behavior research through different studies. Over the past few years, new transactional appliances of performing biomechanical in silico studies have been found highly interesting for orthopedic surgery and traumatol...
Chapter
Defining the best friction model in elastomers for studies with the Finite Element Method (FEM) is a complex task. Hyper-elastic materials have a non-linear mechanical behavior that favors the storage and transfer of deformation energy in an unpredictable way when they are subjected to an external action. This abrupt change determines unpredictable...
Article
Full-text available
Age-related macular degeneration (AMD) is an incurable disease associated with aging that destroys sharp and central vision. Increasing evidence implicates both systemic and local inflammation in the pathogenesis of AMD. Intravitreal injection of anti-vascular endothelial growth factor (VEGF) agents is currently the first-line therapy for choroidal...
Article
Full-text available
In this study, a human hip joint with Cam-type Femoroacetabular Impingement (FAI) is studied by the Finite Element Method (FEM). This pathology consists of a malformation that causes a lack of sphericity of the head of the femur. In turn, this causes wear and tear of the cartilage, a cause of early osteoarthritis of the hip. The objective is to use...
Article
Full-text available
Collapsin response mediator protein 2 (CRMP2) is an adaptor protein that adds tubulin dimers to the growing tip of a microtubule. First described in neurons, it is now considered a ubiquitous protein that intervenes in processes such as cytoskeletal remodeling, synaptic connection and trafficking of voltage channels. Mounting evidence supports that...
Article
Full-text available
Sterculic acid (SA) is a cyclopropenoid fatty acid isolated from Sterculia foetida seeds. This molecule is a well-known inhibitor of SCD1 enzyme, also known as ∆9-desaturase, which main function is related to lipid metabolism. However, recent studies have demonstrated that it also modifies many other pathways and the underlying gene expression. SCD...
Article
Full-text available
Perinatal asphyxia (PA) can cause retinopathy and different degrees of visual loss, including total blindness. In a rat model of PA, we have previously shown a protective effect of hypothermia on the retina when applied simultaneously with the hypoxic insult. In the present work, we evaluated the possible protective effect of hypothermia on the ret...
Article
Full-text available
Apolipoprotein D (Apo D) overexpression is a general finding across neurodegenerative conditions so the role of this apolipoprotein in various neuropathologies such as multiple sclerosis (MS) has aroused a great interest in last years. However, its mode of action, as a promising compound for the development of neuroprotective drugs, is unknown. The...
Conference Paper
Mushrooms have been used for millennia in Eastern traditional medicine as cancer remedies and recent scientific studies have corroborated the presence of anticancer molecules in mushroom extracts. Our goal in this study was to screen a number of scarcely known mushroom species collected in the rain forest of Costa Rica looking for new antitumor mol...
Article
Full-text available
Perinatal asphyxia (PA) is responsible for a large proportion of neonatal deaths and numerous neurological sequelae, including visual dysfunction and blindness. In PA, the retina is exposed to ischemia/reoxygenation, which results in nitric oxide (NO) overproduction and neurotoxicity. We hypothesized that methylene blue (MB), a guanylyl cyclase inh...
Article
Full-text available
In addition to its predominant role in lipid metabolism and body weight control, SCD1 has emerged recently as a potential new target for the treatment of various diseases. Sterculic acid (SA) is a cyclopropene fatty acid with numerous biological activities, generally attributed to its Stearoyl-CoA desaturase (SCD) inhibitory properties. Additional...
Article
Full-text available
In many tissues, stearoyl-CoA desaturase 1 (SCD1) catalyzes the biosynthesis of monounsaturated fatty acids (MUFAS),(i.e., palmitoleate and oleate) from their saturated fatty acid (SFA) precursors (i.e., palmitate and stearate), influencing cellular membrane physiology and signaling, leading to broad effects on human physiology. In addition to its...
Article
This review will focus on the inflammatory and toxic mechanism of action of 7-ketocholesterol (7KCh) and the potential implications of its accumulation, especially in the retina. 7KCh is a pro-inflammatory oxysterol usually associated with oxidized lipoprotein deposits present in aged retinas. High amounts of 7KCh can be generated in situ in these...
Article
Full-text available
Invadopodia are actin-rich protrusions developed by transformed cells in 2D/3D environments that are implicated in extracellular matrix (ECM) remodeling and degradation. These structures have an undoubted association with cancer invasion and metastasis because invadopodium formation in vivo is a key step for intra/extravasation of tumor cells. Inva...
Article
Hypothermia has been proved to have a beneficial effect on several pathologies. CIRBP is one of the so termed cold-shock proteins involved in this process. In this work, we have detected small molecules capable of modulating the activity of CIRBP in the absence of a cold stimulus, by High Throughput Virtual Screening (HTVS) of the Diversity Set IV...
Article
Full-text available
Cancer related deaths are primarily due to tumor metastasis. To facilitate their dissemination to distant sites, cancer cells develop invadopodia, actin-rich protrusions capable of degrading the surrounding extracellular matrix (ECM). We aimed to determine whether β3 integrin participates in invadopodia formed by lung carcinoma cells, based on our...
Data
Invadopodia formation and Mmp involvement in H157 NSCLC. (A) Representative confocal-laser microscopy images of H157 NSCLC cells seeded onto green fluorescent-labelled gelatin for 20 hours, after being exposed or not for 5 days to TGF-β. Actin was visualized by TRICT-phalloidin staining (red). Scale bars 13 μm. (B) Histogram plot quantification of...
Data
β3 integrin blockade affects invadopodia formation in different NSCLC. (A) Quantification of cells presenting active degradation areas as a result of β3 integrin blockade in TGF-β treated and untreated H1299 cells. Cells were pre-treated with 13μg of β3 integrin blocking antibody 2 hours before seeding onto gelatin-coated coverglasses. An isotype n...
Article
Full-text available
Growth factors are extracellular signals that regulate cell proliferation and total body mass. Some animal growth factors can work on plant tissues and vice versa. Here we show that the mammalian growth factor adrenomedullin (AM) induces growth in tobacco plants. Addition of synthetic AM resulted in a dose-dependent growth of tobacco calluses. Furt...
Article
Full-text available
Microfluidic devices are becoming mainstream tools to recapitulate in vitro the behavior of cells and tissues. In this study, we use microfluidic devices filled with hydrogels of mixed collagen-Matrigel composition to study the migration of lung cancer cells under different cancer invasion microenvironments. We present the design of the microfluidi...
Data
Migration experiments. Mean and standard error (parenthesis) of accumulated distance (in microns) after 12 hours of migration, and speed of migration (in microns per hour) in hydrogels C, CM and CM+, with no chemo-attracting substance (Control) using serum containing medium, (20% FBS) or after conjugation with integrin-blocking antibodies (20% FBS...
Data
Control experiments. Mean and standard error (parenthesis) of accumulated distance (in microns) after 12 hours of migration in collagen only hydrogels with increasing crosslinking levels TG6, TG13, and TG26, and with increasing levels of fibronectin TG-F5, TG-F10 and TG-F20, with no chemo-attracting substance (Control) and using serum containing me...
Data
Morphological characterization of the hydrogels used in the control experiments from confocal images. Average and standard deviation (std) of the morphological measurement obtained from Confocal Reflection Microscopy images. The number of samples used to calculate the Fiber length, Fiber persistence, and Pore size is nine (n = 3) since we analyzed...
Data
Time-lapse videos corresponding to an experiment performed in a CM hydrogels with 20%FBS with the calculated tracks overlapped. (AVI)
Data
Diffusion study. Fluorescence intensity captured from a 40kDa-RhodamineB dextran in three parts of the hydrogel H (part of the gel close to the insertion channel), M (middle of the hydrogel), L (part of the gel in the channel opposite to the insertion channel), during 6 hours of experiment, for hydrogels type C (a), CM (b) and CM+ (c). (TIF)
Data
Quantification of H1299 cell migration capability in 3D Transwell experiments. Migration fold, relative to M hydrogels of all the described hydrogels, in the presence or absence of serum. The number of replicas of each experiment is 8 for 20% FBS experiments and 4 for those without serum. *** indicates very statistically significant difference of A...
Data
Polarity plots. Analysis of the average magnitude of the speed of the cells evaluated at different orientations, after re-alignment along the primary migration direction of each track. C (a), CM (b) and CM+ (c). (TIF)
Data
3D Transwell experiments. Representative images used to quantify cell migration in hydrogels C (a), CM (c) and CM+ (e), and in hydrogels made of Matrigel only with the same concentration as in CM, M (b) and CM+, M+ (d). Snapshots show cell migration in the different hydrogels towards 20% FBS. (TIF)
Data
Quantification of H1299 cell migration in hydrogels with increasing levels of cross-linking or fibronectin content. A: Serum free. B: 20%FBS. The number of cells analyzed was: TG-6: 176 (A), 178 (B); TG-13: 271 (A), 303 (B); TG-26 250 (A), 231 (B); TG-F10 162 (A), 211 (B); TG-F20 164 (A), 135 (B). *** indicates very statistically significant differ...
Data
Morphological characterization of the hydrogels used in the control experiments from confocal images. Average and standard deviation (std) of the morphological measurement obtained from Confocal Reflection Microscopy images. The number of samples used to calculate the Fiber length, Fiber persistence, and Pore size is nine (n = 3) since we analyzed...
Data
Morphological characterization of the hydrogels from confocal images. Average and standard deviation (std) of the morphological measurement obtained from the Confocal Reflection Microscopy images. The number of samples used to calculate the Fiber length, Fiber persistence, and Pore size is nine (n = 9) since we analyzed nine sub-images from each ty...
Data
Morphological characterization of the hydrogels from SEM images. Average and standard deviation (std) of the morphological measurement obtained from the SEM images. The number of samples used to calculate the %Porosity, Fiber diameter and Number of pores and is three (n = 3) since we analyzed three images from each type. The number of samples used...
Data
Transwell invasion experiments. Mean and standard error (parenthesis) of number of invading cells in C, CM, and CM+, hydrogels, and in hydrogels with Matrigel only at equal concentration as in CM (M, 2mg/ml) and CM+ (M+, 4mg/ml). The number of replicas of each experiment is 8 for 20%FBS, and 4 for serum free, Control experiments. (DOCX)
Data
Mechanical characterization of the hydrogels. Average (avg) and standard deviation (std) of the storage (G’) and loss (G”) moduli, both in pascal units (Pa) of the control, Matrigel only containing hydrogels. The standard deviation corresponds to three repetitions of each experiment (n = 3). (DOCX)
Data
Time-lapse videos corresponding to an experiment performed in a CM hydrogels with 20%FBS + Anti-β3 with the calculated tracks overlapped. (AVI)
Data
Microdevice design. CAD-ready version of the mask used to fabricate the molds. (DWG)
Data
Segmentation software. A Matlab (Windows x64) executable version of the segmentation coded is provided in S1 Software along with a help file and the necessary DLL libraries. (ZIP)
Conference Paper
The geometry of 3D collagen networks is a key factor that influences the behavior of live cells within extra-cellular matrices. This paper presents a method for automatic quantification of the 3D collagen network geometry with fiber resolution in confocal reflection microscopy images. The proposed method is based on a smoothing filter and binarizat...
Article
Full-text available
Background Transforming Growth Factor beta (TGF-β) acts as a tumor suppressor early in carcinogenesis but turns into tumor promoter in later disease stages. In fact, TGF-β is a known inducer of integrin expression by tumor cells which contributes to cancer metastatic spread and TGF-β inhibition has been shown to attenuate metastasis in mouse models...
Article
Collapsin response mediator protein-2 (CRMP-2) is the first described and most studied member of a family of proteins that mediate the addition of tubulin dimers to the growing microtubule. CRMPs have mainly been studied in the nervous system, but recently, they have been described in other tissues where they participate in vesicle transport, migra...
Article
Full-text available
Dendritic cell (DC) transmigration across the lymphatic endothelium (LEC) is critical for the initiation and sustenance of immune responses. Under non-inflammatory conditions DC transit across LEC has been shown to be integrin-independent. In contrast, there is increasing evidence for the participation of integrins and their ligands in DC transit a...
Article
Full-text available
Nucleocytoplasmic shuttling of Hxk2 induced by glucose levels has been reported recently. Here we present evidence which indicates that Hxk2 nucleocytoplasmic traffic is regulated by phosphorylation and dephosphorylation at serine-14. Moreover, we have identified the protein kinase Snf1 and the protein phosphatase Glc7-Reg1 as novel regulatory part...
Article
Full-text available
Hexokinase 2 (Hxk2) from Saccharomyces cerevisiae was one of the first metabolic enzymes described as a multifunctional protein. Hxk2 has a double subcellular localization and role, it functions as a glycolytic enzyme in the cytoplasm and as a regulator of gene transcription of several Mig1-regulated genes in the nucleus. However, the mechanism by...
Article
Full-text available
Hkx2 (hexokinase 2) from Saccharomyces cerevisiae was one of the first metabolic enzymes described as a multifunctional protein. Hxk2 has a double subcellular localization: it functions as a glycolytic enzyme in the cytoplasm and as a regulator of gene transcription of several Mig1-regulated genes in the nucleus. To get more insights into the struc...
Article
Full-text available
Hexokinase 2 (Hxk2) from Saccharomyces cerevisiae was one of the first metabolic enzymes described as a multifunctional protein. Hxk2 has a double subcellular localization; it functions as a glycolytic enzyme in the cytoplasm and as a regulator of gene transcription of several Mig1-regulated genes in the nucleus. However, the mechanism by which Hxk...
Article
Full-text available
This article is the output of a doctorate course on Molecular Evolution held at the University of Oviedo in Spain in 2005. Students chose by consensus a taxonomic group of their interest (Camelidae), collected sequences from public databases, gathered information on morphological traits, built phylogenies employing different methodologies, compared...
Article
Full-text available
Mig1 and Hxk2 are two major mediators of glucose repression in Saccharomyces cerevisiae. However, the mechanism by which Hxk2 participates in the glucose repression signaling pathway is not completely understood. Recently, it has been demonstrated that Hxk2 interacts with Mig1 to generate a repressor complex located in the nucleus of S. cerevisiae....

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