Rafael MaldonadoUniversity of Puerto Rico at Ponce | UPRP · Department of Biology
Rafael Maldonado
Ph.D
About
11
Publications
514
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48
Citations
Introduction
Additional affiliations
August 2020 - present
Molecular Sciences and Research Center at University of Puerto Rico Rio
Position
- Research Associate
Description
- Ion-Channel Structure and Molecular Biology
Education
May 2021 - May 2021
April 2021 - May 2021
Cognitive class/IBM
Field of study
- Online certification - Python 101 for Data Science
March 2021 - March 2021
BioInteractive
Field of study
- Online certification - Certification hhmi/BioInteractive
Publications
Publications (11)
The main objective of the present study was to find detergents that can maintain the functionality and stability of the Torpedo californica nicotinic acetylcholine receptor (Tc-nAChR). We examined the functionality, stability, and purity analysis of affinity-purified Tc-nAChR solubilized in detergents from the Cyclofos (CF) family [cyclofoscholine...
Since their discovery, nicotinic acetylcholine receptors (nAChRs) have been extensively studied to understand their function, as well as the consequence of alterations leading to disease states. Importantly, these receptors represent pharmacological targets to treat a number of neurological and neurodegenerative disorders. Nevertheless, their thera...
Many pathogens, such as Pseudomonas aeruginosa and Escherichia coli bacteria can easily attach to surfaces and form stable biofilms. The formation of such biofilms in surfaces presents a problem in environmental, biomedical, and industrial processes, among many others. Aiming to provide a plausible solution to this issue, the anionic and hydrophobi...
Over the past 10 years we have been developing a multi-attribute analytical platform that allows for the preparation of milligram amounts of functional, high-pure, and stable Torpedo (muscle-type) nAChR detergent complexes for crystallization purpose. In the present work, we have been able to significantly improve and optimize the purity and yield...
For a long time, traditional purification and extraction methods for the native Torpedo californica nicotinic acetylcholine receptor in lipid-like detergent complex (nAChR-DC) have compromised its purity, functionality and X-ray structural studies possibility. The dataset presented in this article provide a characterization of the Torpedo californi...
Amphetamine-type stimulants (ATS) are the second most consumed illicit drug worldwide and lack good treatments for associated substance use disorders, lagging behind other addictive drugs. For this reason, a deeper understanding of the pharmacodynamics of ATS is required. The present study seeks to determine amphetamine (AMPH) enantiomers’ effects...
Amongst primary brain tumors, gliomas are considered the most lethal malignant tumors due to their resistance to radiation and chemotherapy and the difficulty to accurately localize them within the tissue. Chlorotoxin (Ctx), a small neurotoxin of 36 amino acids isolated from the venom of the Israeli scorpion Leiurus quinquestriatus , binds specific...
One of the limiting factors in the crystallization of membrane proteins is the process of solubilization mediated by detergents. During this process, preservation of the native protein structure and adjacent lipid environments is crucial for appropriate activity and stability. The detergent disruption of lipid–lipid and lipid‐protein interactions a...