Radka Fuchsova

Fakultní nemocnice Plzeň | FN Plzeň · immunoanalytic laboratory

The diagnostics of prostate cancer are currently based on three pillars: prostate biomarker panel, imaging techniques, and histological verification. This paper presents a diagnostic algorithm that can serve as a “road map”: from initial patient stratification to the final decision regarding treatment. The algorithm is based on a review of the curr...

Background: To compare four automated immunoassays for the measurement of 25(OH)-vitamin D (25-OHD) and to assess the impact on the results obtained from a healthy population. Methods: We analysed 100 serum samples on Unicel DxI 800 (Beckman Coulter), Architect i1000 (Abbott), Cobas e411 (Roche) and Liaison XL (DiaSorin). Passing-Bablok regression...

Aim: To compare four automated immunoassays for the measurement of vit25OHD and to assess the impact on the results obtained from a healthy population. Methods: We analysed 100 serum samples on Unicel DxI 800 (Beckman Coulter), Architect i1000 (Abbott), Cobas e411 (Roche) and Liaison XL (DiaSorin). A Wilcoxon signed-rank test was used to compare th...

Aim: Current diagnostics of bone metastatic disease is not satisfactory for early detection or regular process monitoring. The combination of biomarkers and the multiparametric approach was described as effective in other oncology diagnoses. The aim of the study was to improve the difference diagnostics between bone-metastatic disease and solid tum...

Introduction: PSA is a serine protease composed of 240 amino acids in a single polypeptide chain and is a routine parameter in prostate cancer diagnostics. The aim of our study was to test the long-term stability of tPSA and fPSA after 10 years' storage at -80°C. Materials and methods: We analyzed two aliquots from 55 serum samples. The first wa...

Aim: The purpose of this study was to investigate the Prostate Health Index as a marker for tumor aggressiveness in prostate biopsy and the optimization of indication for treatment options. Methods: Our cohort consisted of 320 patients indicated for radical prostatectomy with preoperative measurements of total prostate-specific antigen, free pro...

Aim: The purpose of this study was to investigate the prostate health index as a marker for tumour aggressiveness in prostate biopsy and the optimization of indication for treatment options. Methods: Our cohort consisted of 320 patients indicated for radical prostatectomy with preoperative measurements of total PSA, free PSA, [-2]proPSA and calcula...

Low vitamin D status has been frequently associated with impaired glucose metabolism. We examined associations between 25-hydroxyvitamin D (25-OH-D) and several parameters of glucose homeostasis in virtually healthy subjects, and explored possible interaction with vitamin D receptor (VDR) polymorphism. Nondiabetic subjects without chronic medicatio...

Both vitamins K and D are nutrients with pleiotropic functions in human tissues. The metabolic role of these vitamins overlaps considerably in calcium homeostasis. We analyzed their potential synergetic effect on arterial stiffness. In a cross-sectional study, we analyzed aortic pulse wave velocity (aPWV) in 1023 subjects from the Czech post-MONICA...

Aims: To evaluate the possibility of monitoring the effect of anti TNFa therapy in patients with active rheumatoid arthritis by multiplex assessment of serum levels of different biomarkers which reflects underlying pathogenic mechanisms and activity of this disease. Methods: A group of 23 patients with active rheumatoid arthritis, 20 female, 3 male...

Background: Bone metastases develop in several malignancies (multiple myeloma, breast, prostate and lung carcinoma) and cause several complications. The aim of our study was to search for new biomarkers to use in the monitoring of bone metastatic disease with the use of xMAP technology. Patients and Methods: We assessed 62 oncological patients: 23...

Background: Bone metastases develop in several malignancies (multiple myeloma, breast, prostate and lung carcinoma) and cause several complications. The aim of our study was to search for new biomarkers to use in the monitoring of bone metastatic disease with the use of xMAP technology. Patients and Methods: We assessed 62 oncological patients: 23...

Icodextrin peritoneal dialysis (PD) solution has been shown to increase interleukin-6 (IL-6) levels in PD effluent as well as leukocyte and mesothelial cell count. Mesothelial cells release cancer antigen 125 (CA125), which is used as a marker of mesothelial cell mass. This 1-year prospective study was designed to compare peritoneal effluent cell p...

To evaluate changes in the serum levels of prostate specific antigen (PSA), %free PSA and -2proPSA biomarkers, and prostate health index (PHI) in the diagnostic algorithm of early prostate cancer. The Immunoanalytical Laboratory of the University Hospital in Pilsen examined sera from 263 patients being treated at the Hospital's Urology Department w...

The aim of the present study was to evaluate the usefulness of four interleukins (IL-2, IL-6, IL-8 and IL-10) for melanoma detection and correlate these interleukins with sentinel node metastasis positivity. A group of 236 persons was assessed: 175 patients with melanomas and 61 healthy persons. Melanoma patients were divided to four groups accordi...

IGF1 is responsible for regulation of growth, metabolism, and differentiation of human cells. IGFBP3 is the most abundant of the carrier proteins for IGF1 in the blood. IGF1/IGFBP3 molar ratio is an indicator of IGF1 bioavailability. We decided to create a file of reference ranges of IGF1, IGFBP3 and IGF1/IGFBPP3 ratio for the adult Czech populatio...

Background/aim: There were two aims in the present study. The first was to evaluate the usefulness of insulin-like growth factor 1 (IGF1) for melanoma detection. The second was to correlate changes of serum levels of IGF1 with the Breslow score and sentinel node metastasis positivity. Patients and methods: We examined a group of 216 cases, 77 pa...

Aim: The aim of the present study was to compare the use of cancer antigen 125 (CA125) and human epididymis protein 4 (HE4) biomarkers in patients with endometrial cancer for preoperative management and to particularly focus on relationship between CA125 and HE4 and disease stage in predicting myometrial invasion or intrauterine tumor spread. Thirt...

[No abstract available]

Ovarian cancer is the leading cause of gynecological cancer death. The first aim of our study was to evaluate if HE4 broadens the possibilities in ovarian cancer diagnostics. The second aim was to evaluate the benefits of each biomarker of our panel. We compared the results of following tumor markers: CA 125, HE4, CA 19-9, CEA, TK, TPS, MonoTotal....

Objective: Monitoring changes in the levels of biomarkers PSA, %fPSA, [-2]proPSA and calculation of PHI in the diagnostic algorithm of early prostate cancer. Design: Pilot study Material and Methods: The Immunoanalytical Laboratory of University Hospital in Pilsen examined sera of 76 patients from the Urology department of the University Hospital w...

Objective: Monitoring changes in the levels of biomarkers PSA, %fPSA, [-2]proPSA and calculation of PHI in the diagnostic algorithm of early prostate cancer. Material and Methods: The Immunoanalytical Laboratory of University Hospital in Pilsen examined sera of 76 patients from the Urology department of the University Hospital with suspected prosta...

Objective: Monitoring and evaluation of the benefit of ROMA indexes calculation in ovarian cancer and comparison with values of serum tumor markers HE4 and CA125 Material and Methods: In the period from June 2010 to December 2011 was in the Immunoanalytical laboratory, University Hospital Pilsen serum of 553 patients from department of Gynecology a...

The aim of the study was to investigate the benefi ts of the calculation of the Risk Ovarian Malignancy Algorithm (ROMA) index for the primary detection of ovarian cancer in a population of Czech women. Aware of the age distribution of ovarian cancer, we focused on post-menopausal patients. In conclusion the HE4 together with that of CA125 determin...

The aims of our project were to evaluate the benefi ts of the determination of human epididymis protein 4 (HE4) and compare the results with the following tumor markers: CA 125, CA 19-9, CEA, TK, TPS and MonoTotal. In conclusion HE4 levels, together with CA125 and CEA broadens the range of differential diagnostic possibilities for distinguishing be...

Objective: Verification of the importance of determination HE4 and calculation of ROMA index for increasing the efficiency of diagnosis of ovarian cancer in a population of Czech women. Methods: In the period from 06/24/2010 to 12/01/2011 was at the Department of Gynaecology and Obstetrics, University Hospital Pilsen examined 552 patients with abno...

Objective: Monitoring of changes in the serum levels of HE4, CA 125, CEA and CA 19 -9 in ovarian cancer and diseases that can be considered in the differential diagnosis. Material and Methods: In the period from June 2010 to December 2011 was in the Immunoanalytical laboratory, University Hospital Pilsen serum of 749 patients examined. These patie...

Aim: The first aim of the project was to evaluate the benefits of the determination of human epididymis protein 4 (HE4) and the risk of ovarian malignancy algorithm (ROMA) index for primary detection of ovarian cancer in a population of Czech women. The second aim was to study the advantages HE4, cancer antigen 125 (CA125) and ROMA index for distin...

The first aim of this project was to study new possibilities for distinguishing benign from malignant tumors using growth factors and to compare them with the traditional tumor markers Carcinoembryonic antigen (CEA) and Cancer antigen 15-3 (CA15-3) for breast tumors. The second aim was to make a comparison of CEA, CA 15-3, Insulin-like growth facto...

Many studies have demonstrated the relationship between vitamin D and cancer of many different sites, including of the breast, colorectum, prostate and lung. Most epidemiological studies have assessed the effects of dietary intake only, although endogenous production after sun exposure is the main source of vitamin D. The aim of our pilot study was...

A new cytokeratin tumor marker, MonoTotal was studied in lung cancer, the most common cause of cancer mortality worldwide. In non-small cell lung cancer (NSCLC) patients MonoTotal serum levels and their relationship to the tumor stage, histological subtype, early relapse and cancer-related death were evaluated. MonoTotal serum levels were studied,...