Rachid Benkirane

Rachid Benkirane
Université Ibn Tofail · Department of Biology

Professor

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214
Publications
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Publications

Publications (214)
Chapter
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Despite the importance of arbuscular mycorrhizal fungi (AMF) within forest and agroecosystems, few data are available about how AMF communities are structured in the root zone of the argan tree. Some studies have characterized endomycorrhizal fungi population occurring in rhizosphere soils of argan trees grown in southwest of Morocco, numerous site...
Article
Rice ( Oryza sativa L.) is the staple food of more than half of the world population. However, its production is facing several biotic constraints. Among serious biotic factors that harm rice crops, the Helminthosporium disease has severe adverse impacts on rice yield, generating heavy losses of up to 90%. Four Bipolaris oryzae isolates were recove...
Article
Full-text available
Grapevine crown gall, caused by Allorhizobium vitis (A. vitis) and fire blight, caused by Erwinia amylovora (E. amylovora) of rosaceous plants, have been recognized as serious bacterial diseases and responsible for major crop losses in grapevine (Vitis vinifera L.), pear (Pyrus communis L.) and apple (Malus domestica Borkh.) worldwide. To develop a...
Article
Grapevine crown gall, caused by Allorhizobium vitis (A. vitis) and fire blight, caused by Erwinia amylovora (E. amylovora) of rosaceous plants, have been recognized as serious bacterial diseases and responsible for major crop losses in grapevine (Vitis vinifera L.), pear (Pyrus communis L.) and apple (Malus domestica Borkh.) worldwide. To develop a...
Article
This study aimed to evaluate the effect of plant growth-promoting rhizobacteria (PGPR) and compost, applied alone or in combination, on tomato resistance to vascular wilt caused by Verticillium dahliae. Improved management of vascular wilt is important for enhanced productivity of greenhouse-grown susceptible tomato plants. The application of compo...
Article
Full-text available
Soil and root samples were collected from the rhizo-sphere of planted and natural argan trees growing in the Bounaga and Smimou sites, respectively, in northwest Morocco. Frequency and intensity of my-corrhization and arbuscular content of the roots of argan trees varied between sites. Spore morphotypes on the two sites belonged to 14 species and 6...
Article
The present work aimed to investigate the effect of potentially beneficial soil microorganisms in enhancing the tolerance of tomato plants against Verticillium wilt. The autochthonous mycorrhizal fungi "Rhizolive consortium" and plant growth-promoting rhizobacteria (PGPR) boost the growth and development of plants by providing well-adopted strategi...
Article
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This paper lists the current fauna of Aphrophoridae family in Morocco. Distribution data of their occurrence in the country are provided. Nine species of aphrophorid spittlebugs found in Morocco are listed, five of them identified and described for the first time in the country and four other species reported in the literature. An identification ke...
Article
Saffron cultivation is a viable alternative for marginal areas where low soil fertility and water availability severely limit the cultivation of other crops with higher water and input requirments. Under these conditions, arbuscular mycorrhizal fungi (AMF) are an essential alternative for maintaining fertility and water conservation, stimulating gr...
Article
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Sustainable barley ( Hordeum vulgare L.) production will require access to diverse ex-situ conserved collections to develop varieties with high yields and capable of overcoming the challenges imposed by major abiotic and biotic stresses. This study aimed at searching efficient approaches for the identification of new sources of resistance to barley...
Article
Full-text available
The present study aims to assess the effect of autochthonous mycorrhizal fungi "Rhizolive consortium" on Verticillium wilt of tomato plants (Lycoperscum esculentum Mill.) and induction of antioxidant enzymes in response to Verticillium dahliae inoculation. 15 days old tomato plants were inoculated first with Rhizolive consortium for two months then...
Article
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Rapid, easy and direct DNA extraction protocols are required for use in routine analyses to detect plant pathogenic bacteria. For this purpose, five protocols for extracting bacterial DNA, which do not use toxic chemicals such as phenol and chloroform, were optimized for the detection of plant pathogenic bacteria quickly and directly from plant mat...
Article
This study aims to investigate the chemical composition and inhibitory effects of four essential oils from Moroccan medicinal and aromatic plants: Rosmarinus officinalis L., Satureja calamintha L., Lavandula stoechas L. and Citrus aurantium L. on Allorhizobium vitis strain S4 causal agent of crown gall of grapevine. The identification of compounds...
Article
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The bacterium Xylella fastidiosa represents a potential risk to the Moroccan flora. For this reason, several surveys were conducted in 2019 and 2020 to assess its occurrence and to establish an inventory of its potential insect vectors with emphasis on their characterization and importance from 85 sites in 25 provinces across the country. Using a s...
Article
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The study aims to evaluate the effect of endomycorrhizal inoculum (arbuscular mycorrhizal fungi), phospho-compost and phosphate sludge in single (M, PC, PS) or dual combinations (PC+M, PS+M, PS+PC) compared to agricultural and Mamora soils (A and S) on the growth, flowering, and yield of tomato plants. Among the studied treatments, the substrates con...
Preprint
Full-text available
Sustainable barley production will require access to diverse ex-situ conserved collections to develop varieties with high yields and capable of overcoming the challenges imposed by major abiotic and biotic stresses. This study aimed at searching efficient approaches for the identification of new sources of resistance to barley leaf rust (LR). Two s...
Article
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The ability of agriculture to continuously feed a growing world population depends essentially on the use of effective and sustainable strategies to control crop diseases. Currently used bacterial infection control strategies, involving antibiotics, pose a real threat to the environment and to human health, and are losing efficiency due to the appe...
Article
The combination of biotic and abiotic stresses in semiarid regions results in severe losses in citrus production. Neglecting the interactions that may occur between these factors would lead to the over or underprediction of the performance of rootstock cultivars under natural conditions and therefore to their erroneous ranking. In this study, seven...
Article
Full-text available
Although some studies have characterized endomycorrhizal fungi population occuring in rhizosphere soils of argan trees grown in the southwest of Morocco, numerous sites of this area remained unexplored. There is a need to study additional soil samples which cover more sites of argan tree stands. Hence, we studied endomycorrhizae diversity and the m...
Article
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The great morphological similarities within the Colletotrichum genus and the lack of precision of classical identification methods have made the use of molecular methods mandatory. Despite the great diversity of species within the Colletotrichum genus, very few morphological characters are expressed. Morphological examination of the isolates of Col...
Article
The fruits juice of 61 mandarins (Citrus reticulata Blanco) germplasm from the citrus fruits collection planted in the experimental field of National Institute for Agricultural Research (INRA) of Kenitra in Morocco were analysed. From principal component analysis, a selection based on the study of the chemical characters of sixty-one varieties allo...
Article
Full-text available
The fruits juice of 61 mandarins (Citrus reticulata Blanco) germplasm from the citrus fruits collection planted in the experimental field of National Institute for Agricultural Research (INRA) of Kenitra in Morocco were analysed. From principal component analysis, a selection based on the study of the chemical characters of sixty-one varieties allo...
Article
The impact of soil amendment with compost and/or application of arbuscular mycorrhizal fungi (AMF) on the resistance of tomato to Verticillium dahliae was evaluated. The frequency and intensity of mycorrhization decreased in V. dahliae inoculated plants. Application of compost and AMF significantly improved plant growth, stomatal conductance and ch...
Article
Full-text available
Surveys were carried out in April-May, 2017 in plots of barley located in different localities of northwest Morocco. Root and soil samples were taken under barley feet growing in these plots. The spores were isolated from the soil samples by wet sieving and the roots were stained with Cresyl blue. The observation of barley plants roots fragments fr...
Article
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Barley is a crop of global significance with multiple uses (feed, food and beverages), but its productivity worldwide is limited by several abiotic and biotic stresses. Crop wild relatives, exclusively, Hordeum vulgare ssp. spontaneum and H. bulbosum, are important genetic resources needed to develop new adapted varieties. A total of 117 accessions...
Article
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The isolates TR-B 98 (2) and TR-B 98 (3) from the phosphate mines of Morocco and the isolate Trichoderma asperellum were tested for their ability to survive in the soil and stimulate the growth of wheat plants (soft wheat and durum wheat) and barley on a Mamora (SM) sand-based growing medium modified with increasing concentrations of natural phosph...
Article
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Argan tree (Argania spinosa (L.) Skeels) is a medium-sized, thorny tree endemic to the semi-desert Souss valley of southwestern Morocco. Cultivated for oil and fodder, it is considered the most important plant species in North Africa from both economic and ecological standpoints. In the present work, argan seeds originating from two producing regio...
Article
Full-text available
Biological and chemical treatments of seeds with a Trichoderma suspension or solutions of Thirame with 10% gelatin, 4% superphosphate, and 0.1% sucrose were able to reduce the severity, incidence and index of root rot in wheat and barley plants growing on a substrate inoculated with three pathogens responsible for root rot, Curvularia spicifera, Bi...
Article
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The objective of the present paper was to evaluate the effect of nitrogen level on yieldand fruit qualityof citrusand to determine the optimum N fertilizer ratesof Navel orangevarietybudded on Troyercitrange rootstock in field conditions. The orange Navel orchard spaced7mx7m (204 trees/ha) grownin 1964on a sandy soilat the experimental station of E...
Article
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Mineral nutrition is one of the most important components of citrus production management. The volume of production and fruit quality are closely related to mineral nutrition given to citrus trees. The objective of the present study was to evaluate the effect of different nitrogen, phosphorus and potassium levels on yield, fruit size, fruit weight...
Article
Full-text available
This study demonstrated beneficial effects of the symbiotic relationship between mycorrhizal arbus-cular fungi (AM) and olive trees. Young olive trees were treated with a composite mycorrhiza treatment with and without inoculation of the pathogenic Phy-tophthora palmivora. A non-inoculated control was also included. Mycorrhizal plants had greater m...
Article
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The attempts of argan tree (Agrania spinosa (L.) Skeels) reforestation programs, as a multi-use agro-forestry species deal with the mastering the production of superior quality seedlings in nurseries. Trichoderma spp. are well known as soil inhabitant and endophytic plant symbionts that are widely used as seed treatments to enhance plant growth. In...
Poster
Full-text available
Crown gall is the most important grapevine bacterial disease throughout the world. It is caused by the plant pathogen Allorhizobium vitis that is present in several regions of vine production in the world (Ophel and Kerr 1990). This disease is a kind of “plant cancer”, where the bacterium hijacks the plant’s cellular-level machinery and manipulates...
Article
Full-text available
Pure cultures of a large number of fungal isolates were obtained from anthracnose symptoms developed on the olives collected from Ouazzane and Sidi Kacem regions (North and North West of Morocco). Microscopic observations, based on morphological criteria, identified some of them belonging to the genus Colletotrichum. However, high morphological sim...
Article
Full-text available
The attempts of argan tree (Agrania spinosa (L.) Skeels) reforestation programs, as a multi-use agro-forestry species deal with the mastering the production of superior quality seedlings in nurseries. Trichoderma spp. are well known as soil inhabitant and endophytic plant symbionts that are widely used as seed treatments to enhance plant growth. In...
Article
Full-text available
The treatment of Citrus aurantium L. seeds with an endomycorrhizal consortium, originally from the rhizosphere of different species of citrus allowed roots mycorrhization and improvement of all agronomic parameters of the plants resulting from the germination of these seeds following coated seeds germination compared to those of the plants develope...
Article
Full-text available
To ensure sustainable management of limiting soil P and minimize the extensive use of mineral fertilizers, friendly environmental agricultural practices are required. In this study, the effects of phospho-compost and phosphate sludges either alone or combined with an endomycorrhizal inoculum were tested on the growth parameters of maize plants. Gre...
Article
Full-text available
The attempts of argan tree (Agrania spinosa (L.) Skeels) reforestation programs, as a multi-use agro-forestry species deal with the mastering the production of superior quality seedlings in nurseries. Trichoderma spp. are well known as soil inhabitant and endophytic plant symbionts that are widely used as seed treatments to enhance plant growth. In...
Article
The objective of this study is to demonstrate the effect of different doses of phospho-compost (2.5%; 5%; 7.5%; 10%) on the growth and development of the bean plants and to see the ability of endomycorrhizal fungi contained in sludge, an essential component of this phospho-compost, to mycorrhize these seedlings. The obtained results showed that the...
Article
Full-text available
The pathogenicity of Rhizoctonia solani isolates derived from strawberry plants was evaluated against ten crops mostly grown into or closely to strawberry plantation areas plots. 13 weeks after inoculation, strawberry plants showed symptoms when they were inoculated by making collar injury using wooden toothpick whereas those inoculated by using ro...
Article
Full-text available
Surveys carried out in wheat (hard and soft) and barley fields in April-May 2017 in northwestern Morocco revealed the presence of root rot symptoms in all prospected wheat and barley plots. The detailed examination of the plants brought back to the laboratory (wheat and barley) revealed the presence of necrotic lesions or black lesions on the roots...
Article
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The Moroccan strain of Trichoderma asperellum Banklt1902509 SMis 1 KU987252 RAB 95369 isolated from compost and the pathogenic fungus Verticillium dahliae were tested for their tolerance to heavy metals. The growth behavior of these two fungal species and biocontrol ability of T. asperellum against Verticillium dahliae were investigated as a functi...
Article
Full-text available
Aims: Crown gall, a phytobacteriosis characterized by the formation of tumours on plant roots was observed in recently planted vineyards of the Meknes region (Morocco). The objective of this research was to analyze the diversity of pathogenic agrobacteria isolated from grapevine in Morocco. Methods and results: Eighty-two isolates from 11 affect...
Article
The Gram-positive bacterium Clavibacter michiganensis subsp. Michiganensis (Cmm), causal agent of bacterial wilt and canker of tomato, is an economically devastating pathogen that inflicts considerable damage throughout all major tomato producing regions. Three rhizobacteria strains Bacillus cereus, Pantoea agglomerans and Serratia proteamaculans w...
Article
Full-text available
The effect of a composite endomycorrhizal inoculum is tested on the occurrence of Fusarium wilt, caused by Fusarium solani, in chickpea plants. Eight weeks after inoculation, the aerial part’s length (24 cm), aerial part’s fresh weight (4.5 g), fresh root weight (5.54 g), leaves’ number (22) and the number of pods (4) are higher than those found in...
Article
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Aims: This study aimed to i) identify Pseudomonas savastanoi pv. savastanoi (Pss) as a causal agent of the olive knot on the basis of biochemical, pathogenicity and PCR technique ii) investigate in vitro bacterial resistance toward copper-based compounds and efficiency of some antibiotics on pathogen suppression. Methodology and results: Biochemica...
Article
Full-text available
Surveys were carried out in wheat (durum wheat and soft wheat) and barley fields in April-May 2017 in northwestern Morocco allowed the isolation of a species of the Curvularia genus from the characteristic necrotic lesions of the root rot of wheat and barley. This fungus was isolated either in the presence of Bipolaris sorokiniana or among a variab...
Article
Full-text available
The number of infectious propagules of arbuscular mycorrhizal fungi (AMF) in phosphate sludge was estimated using the most probable number (MPN) method, and compared with the results obtained by wet sieving techniques and the technique of staining roots. In the sludge soils of phosphates D, C, E, Bassin F and the rhizosphere soils of olive trees, a...
Article
Full-text available
This work aims to demonstrate the pathogenicity of 37 isolates of Fusariumspecies and 4 isolates of Bipolaris sorokinianaon durum wheat (Karim and Amjad varieties), soft wheat (Wafia and Amal varieties) and barley (Hordeum pusillumand Hordeum escourgeon).All isolates induced root necrosis in inoculated wheat and barley plants. Isolates F10 (F. sola...
Article
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The present study was undertaken to estimate in vitro the capacity of twenty-three isolates of Trichoderma, isolated from soil and roots collected from adjacent sites to the Khouribga and Benguerir Phosphate mines (Morocco), and seven isolates from the laboratory's mycoteca to solubilize the Tricalcium phosphate. All these isolates showed, after th...
Article
Full-text available
The diversity of endomycorrhizal fungi in the rhizosphere of Crocus sativus has been studied at five sites in the Taliouine region (Tinfat), located in Taroudant Province (Morocco), according to the number of years of soil exploitation by Saffron cultivation. In all sites, the roots of Crocus sativus carry structures of endomycorrhizal fungi. Root...
Article
Full-text available
Objective: The objective of this study is to know the diversity of arbuscular mycorrhizal fungi in the soil sites adjacent to Khouribga phosphate mines (Morocco) Methods: Soil samples were collected from two localities, Hattaneand Merah Lahrach, in Khouribga Region (Morocco) nearby two operated sites of phosphates extraction. Five samples were rand...
Article
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Keywords: Pseudomonas savastanoi pv. savastanoi, olive knot disease, biological control, antagonistic bacteria, Bacillus subtilis