Rachel Churm

Rachel Churm
Swansea University | SWAN · A-STEM

PhD

About

18
Publications
2,840
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122
Citations

Publications

Publications (18)
Article
Full-text available
As elite athletes demonstrate through the Olympic motto ‘citius, altius, fortius’, new performance records are driven forward by favourable skeletal muscle bioenergetics, cardiorespiratory, and endocrine system adaptations. At a recreational level, regular physical activity is an effective non‐pharmacological therapy in the treatment of many endocr...
Article
Full-text available
Regular physical activity reduces the risk of several site-specific cancers in humans and suppresses tumour growth in animal models. The mechanisms through which exercise reduces tumour growth remain incompletely understood, but an intriguing and accumulating body of evidence suggests that the incubation of cancer cells with post-exercise serum can...
Article
Purpose The aim of this study was to assess the relationship between specific erythrocyte fatty acids levels and vascular health in type 1 diabetes (T1D) with and without insulin resistance (IR). Methods We analysed baseline pretreatment data in a subset of 23 patients with T1D from a previously published randomised controlled trial consisting of...
Article
Introduction: This study sought to compare the metabolomic, hormonal and physiological responses to hypoglycemia versus euglycemia during exercise in adults with type 1 diabetes (T1D). Research design and methods: Thirteen individuals with T1D (hemoglobin; 7.0%±1.3% (52.6±13.9 mmol/mol), age; 36±15 years, duration diabetes; 15±12 years) performe...
Article
Full-text available
Background: The role of omega-3 polyunsaturated fatty acids (n-3PUFA), and the potential impact of n-3PUFA supplementation, in the treatment and management of type 1 diabetes (T1D) remains unclear and controversial. Therefore, this study aimed to examine the efficacy of daily high-dose-bolus n-3PUFA supplementation on vascular health, glycaemic co...
Article
Aim To detail the extent and prevalence of post-exercise and nocturnal hypoglycemia following peri-exercise bolus insulin dose adjustments in individuals with type 1 diabetes (T1D) using multiple daily injections of insulins aspart (IAsp) and degludec (IDeg). Methods Sixteen individuals with T1D, completed a single-centred, randomised, four-period...
Article
Full-text available
Background Bariatric surgery is an effective treatment for morbid obesity and glycaemic dysfunction.Objectives The aim of the work was to examine both the static and dynamic changes of glucose-insulin homeostasis and incretin hormone response following sleeve gastrectomy (SG) in a sample of 55 participants preoperatively and 1 month and 6 months po...
Article
Full-text available
Background Previous studies have examined changes in plasma markers of inflammation and oxidative stress up to 24 months following bariatric surgery, but there is limited evidence on the long-term effects of bariatric surgery. Objectives To examine the effects of bariatric surgery on adipokines (adiponectin, leptin), inflammatory cytokines [C-reac...
Article
Full-text available
Introduction There is limited literature available on the long-term effect of bariatric surgery especially laparoscopic sleeve gastrectomy (LSG) on the incretin hormone response. Aim Our primary aim was to investigate changes in glucose metabolism and incretin hormone responses in participants with impaired glucose regulation approximately 4 years...
Article
Background: Previously developed in vitro cultures of the human retina have been solo or dual cell cultures. We developed a triple-cell culture in vitro model utilizing a membrane system to produce a better representation of a functional and morphological human retina. Methods: Retinal microvascular endothelial cells (HRMVEC/ACBRI181, Cell syste...
Article
Background: Bariatric surgery is an effective treatment for morbid obesity and metabolic dysfunction. Objectives: The aim of this work was to examine the early temporal effects of laparoscopic sleeve gastrectomy (LSG) on adipokines (adiponectin, leptin), inflammatory cytokines (interleukin-6, C-reactive protein, interleukin-10), and global plasm...
Article
Acyl-ghrelin has various peripheral effects including the potential role in mediating cellular lipid removal and macrophage polarization. Previous reports are contradictory as to how glycaemia and acyl-ghrelin mediates lipid retention and inflammation within individuals with Type 2 diabetes (T2D). Our aim was to explore acyl-ghrelin levels and ghre...
Poster
Background & aims: Peroxisome proliferator-activated receptor gamma (PPAR-ɣ) induces the removal of cellular lipids by high density lipoproteins via the activation of liver X receptor isoform (LXRβ) and in turn ATP binding cassette G1 (ABCG1). Centrally acting acyl ghrelin has been implicated as having a detrimental effect on the transcription of t...
Article
Full-text available
Aims: As the use of Point of Care Testing (POCT) devices for measurement of glycated haemoglobin (HbA1c) increases, it is imperative to determine how their performance compares to laboratory methods. This study compared the performance of the automated Quo-Test POCT device (EKF Diagnostics), which uses boronate fluorescence quenching technology, w...
Article
The 28 amino acid hormone, ghrelin, has been found to have various effects on metabolism. This review will focus on the pathways integrated into ghrelin's effect within the hypothalamus, pancreas and adipocytes. The identification of molecules and pathways that regulate ghrelin-mediated lipid retention could establish new mechanisms underlying cell...
Article
Full-text available
Background and Aims: Cycloxygenase-2 (COX-2) catalyses the rate limiting step of prostaglandin biosynthesis. Despite previous studies, it is still unclear whether COX-2 is beneficial or detrimental to cardiovascular risk. The aim of this study was to examine the -765G>C (rs20417) PTGS2 promoter gene variant, which encodes COX-2, in relation to mark...
Poster
Full-text available
ABCG1 is involved in mediating cholesterol efflux and modulating cellular lipid homeostasis. Its transcription is regulated by nuclear cholesterol sensors (LXR) and influenced by endogenous glucose levels. Our aim was to examine the expression of genes involved in pathways related to cholesterol efflux in visceral fat samples obtained from non-obes...

Questions

Question (1)
Question
Hey,
I have done optimization of my primers to produce the correct programme. After running the real time I was successful in producing efficiencies for 3 primers (E= 95%-105%). However, when repeating what I had previously achieved the efficiencies they have now shot up to >500% on these 3 primers. Also, this week I have continued with looking at the other primers but I seem to be getting negative values for the efficiencies on 2 primers i.e.E= -99.6% and E=-97.9%. 
I have also run a NTC alongside the dilutions to ensure no contamination and this was clear. 
Can anyone suggest where I may possible be going wrong?
Please & thank you!

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Projects

Project (1)
Project
(i) To examine the role played by ghrelin as a mediator of lipid retention in a human adipocyte cell line. (ii) To characterise the expression of specific lipid retention genes in visceral and subcutaneous adipose depots derived from subjects who are lean (L), obese (O) and obese with T2DM (ODM). (iii) To establish the effect of ghrelin mediated lipid retention on adipocyte function with focus on the inflammatory cytokines.