Qutaiba shuaib Al-Nema

University of Mosul · Department of Biology

Protoplasts represent a unique experimental system for the circulation and formation of genetically modified plants. Here, protoplasts were isolated from genetically modified hairy root tissues of Brassica oleracea L. induced by the Agrobacterium rhizogenes strain (ATCC13332). The concentration of enzyme solutions utilized for protoplast isolation...

انوةدإ دج الد ا رسدة الحالردة نباتدات البروتمدج Brassica oleracea var. italica ةدؽ تطايز تالس أر ا ز باد ا رتيا الةذ ورت السدرقان اولأو ا ر (. قدد نةدح تدالس الاو ا ر والسدرقان الظاةردة عمى وسط ةؾ ا رفدر وسدكؾ) MS - ( الصدم الطددعؼ د 2.5 ةمغدؼ لودر 1 6-Benzyl adenine (BA) - و 2.2 ةمغدؼ لودر 1 Naphthalene Acetic- Acid (NAA) وتدالس الةدذور الظداةج عم...

This study was able to produce a transgenic plant of Broccoli, Brassica Oleracea var. Italica, by using the bacterial strain Agrobacterium rhizogenes ATCC13332 and its plasmids, which produced morphogenesis of genetically transformed hairy roots from stems and leaves. The better response of hairy roots formation was shown at leaves, which inoculate...

إن نمو فكرة تأليف هذا الكتاب، الذي يتناول احد المكونات المهمة في الخلية، تبلورت اثناء تدريسنا في السنوات العشرة الماضية مجموعة من موضوعات التقانات الحيوية الحديثة في النبات ضمن برامج الدراسات الاولية والعليا. وفضلاً عن ذلك فقد انجزت في مختبرات وحدة التقنيات الحياتية مجموعة من البحوث في هذه الموضوعات ونشرت في مجلات عالمية وعربية ومحلية متخصصة، كما ا...

Broccoli, Brassica oleracea var.italica is an important plant due to its flavor and anticancer activity that received more attention in recent years. The leaf, stem and root explants of aseptic 15 day old seedlings, grown on MS medium were used for callus initiation.Theseexplants were cultured on agarsolidified MS medium containingBAP, NAA, TDZ pla...

Allium sativum ‫و‬ ‫محتو‬ ‫اه‬ ‫من‬ ‫النووي‬ ‫الحامض‬ DNA ‫المالح‬ ‫قاسم‬ ‫احم‬ ‫مز‬ ‫و‬ ‫النعمة‬ ‫شعيب‬ ‫قتيبة‬ ‫و‬ ‫الكاتب‬ ‫أسامة‬ ‫ا‬ ‫مر‬ ‫الحياة‬ ‫علوم‬ ‫قسم‬ ، ‫بية‬ ‫التر‬ ‫كلية‬ ، ‫الموصل‬ ‫جامعة‬ ، ‫الموصل‬ ، ‫اق‬ ‫العر‬ ‫االستالم:‬ ‫يخ‬ ‫تار‬ (71 / 2 / 2000 ‫القبول:‬ ‫يخ‬ ‫تار‬ ، 22 / 6 / 2000) ‫الملخص:‬ ‫اس‬ ‫ار‬ ‫ر‬ ‫م‬ ‫تحدثت‬ ‫وم‬ ‫ا...

This study was able to produce a transgenic plant of Broccoli, Brassica Oleracea var. Italica, by using the bacterial strain Agrobacterium rhizogenes ATCC13332 and its plasmids, which produced morphogenesis of genetically transformed hairy roots from stems and leaves. The better response of hairy roots formation was shown at leaves, which inoculate...

In this study protoplasts were successfully isolated from leave's mesophyll of two varieties of sugarbeet (Beta vulgaris L.). Various enzyme solutions were assessed for their cell wall degrading ability, which express different efficiency in isolation of protoplasts from leaf mesophyll of var. Baraka. The yield of protoplasts isolated was 18 × 10 4...

Algae biofuels is considered as an alternative source to fossil fuels. In recent decades, there was a significant increase in the use of energy sources in order to avoid the depletion of traditional sources such as coal and petroleum. The produced fuel from algal oil had important characteristics compared to that from other vegetable crops. This is...

Abstract Broccoli, Brassica oleracea var.italica is an important plant due to its flavor and anticancer activity that received more attention in recent years. The leaf, stem and root explants of aseptic 15 day old seedlings, grown on MS medium were used for callus initiation.Theseexplants were cultured on agarsolidified MS medium containingBAP, NAA...

Abstract: Ti-plasmid (Tumor inducing plasmid) was isolated from Agrobacterium tumefaciens Binn A6. Cell suspension was established from leaves callus of Dianthus caryophyllus in liquid Murashige and Skoog (MS) medium supplemented with 0.5 mg / L 2,4-D and 0.1 mg / L BA. The favorite density were ( 383 × 103 and 420 × 103 cell / ml). They mixed with...

Algae biofuels is considered as an alternative source to fossil fuels. In recent ‎decades, there was a ‎significant increase in the use of energy sources in order ‎to avoid the depletion of traditional ‎sources such as coal and petroleum. The ‎produced fuel from algal oil had important ‎characteristics compared to that ‎from other vegetable crops....

In this investigation hairy roots were induced on leave petioles of Ruta graveolens L. plants. These roots were white in color and negative geotropism. Paper electrophoresis of hairy roots extract resulted in the separation of black spots of agropine. These transformed roots were cured from Agrobacterium rhizogenes, some cultures of these roots wer...

Genetic transformation using Agrobacterium is one of the techniques used to transfer desired genes to plants. This protocol is considered a shortcut to get transformed plants which could be an alternative method and suitable system compared with the classical methods. This study aimed to investigate the response of C. annuum seedlings (sweet variet...

Genetic transformation using Agrobacterium is one of the techniques used to transfer desired genes to plants. This protocol is considered a short – cut to get transformed plants which could be an alternative method and suitable system compared with the classical methods. This study aimed to investigate the response of C. annuum seedlings (sweet var...

Somatic hybridization between different plants through protoplast fusion represent an efficient experimental approach to produce genetically transformed plant species. Electrofution of mesophyll protoplasts in sugar beet was occurred to overcome the barriers faced breeding program of this economically industrial crop Protoplasts were successfully i...

The clones (C1, C2, C3 and C4) of sugarbeet leaves express their capability to induce the adventitious roots as a result of their injection with suspension of Agrobacterium rhizogenes R1601. Clear differences were found in the response and transgenesis of these explants. The result indicated that C3 was the best in this case, but hairy roots produc...

Protoplasts were isolated from leaf mesophyll of sugarbeet (Beta vulgaris L.) axenic seedlings. Eight enzyme mixtures were tested for cell wall degrading ability. The efficient enzyme solution was mixture "II" that consist of 1.5% Cellulase RS, 2% Cellulase R10, 1% Macerozym R10 and 0.1% Pectolyase Y23. This mixture was efficient in releasing proto...

Protoplasts were isolated from leaf mesophyll of sugarbeet (Beta vulgaris L.) axenic seedlings. Eight enzyme mixtures were tested for cell wall degrading ability. The efficient enzyme solution was mixture "II" that consist of 1.5% Cellulase RS, 2% Cellulase R10, 1% Macerozym R10 and 0.1% Pectolyase Y23. This mixture was efficient in releasing proto...

Hairy roots were induced on leaf explants excised from sugarbeet (Beta vulgaris) plants when inoculated by Agrobacterium rhizogenes R1601. Paper electrophoresis proved the presence of agropine, the unusual amino acid in these roots which demonstrated the transformation of these tissues. Transformed hairy roots were easily grown on agar-solidified M...

In the present study, transformed hairy root were produced on true leaves of sugar beet (Beta vulgaris) plants by direct inoculation with Agrobacterium rhizogenes R1601 containing Ri plasmid. Leaves responded highly to form these root compared with petioles. The hairy roots initated at the site of inoculation were white in color, with a larg number...