Quentin Raffy

University of Strasbourg | UNISTRA · Institut Pluridisciplinaire Hubert Curien (IPHC)

The aim of this work is to estimate the biological effect of targeted radionuclide therapy using Cu-64, which is a well-known Auger electron emitter. To do so, we evaluate the absorbed dose of emitted particles from Cu-64 using the Geant4-DNA Monte Carlo simulation toolkit. The contribution of beta particles to the absorbed dose is higher than that...

We evaluate the track segment yield G′ of typical water radiolysis products (e aq ⁻ , · OH and H 2 O 2 ) under heavy ions (He, C and Fe ions) using a Monte Carlo simulation code in the Geant4-DNA. Furthermore, we reproduce experimental results of · OH of He and C ions around the Bragg peak energies (< 6 MeV/u). In the relatively high energy region...

Radiation chemical yields (G value) of OH radicals created in the accelerator-based neutron field are evaluated using coumarin-3-carboxilic acid (C3CA) solution to understand the role of the indirect action induced by radiations emitted as a result of ¹⁰B(n,α)⁷Li reactions for boron neutron capture therapy. The G values for ionizing radiations emit...

It has been confirmed that the density of carbonate ester in poly(allyl diglycol carbonate) (PADC), which is a well-known polymeric nuclear track detector CR-39, begins decreasing above a critical dose of about 60 kGy during exposures to Co-60 gamma rays and Ultra Soft X-rays (USX: Kα (Al) with energy 1.49 keV). After exposure to Co-60 gamma rays a...

We report the loss of carbonate ester and the creation of embedded CO 2 molecules and OH groups in poly (allyl diglycol carbonate) (PADC) irradiated with C (11 MeV/u) and O ion (7.5 MeV/u) beams under vacuum at low temperatures (11, 100 and 200 K) and at room temperature (RT) using in-situ FT-IR spectrometry. For comparison with these experiments,...

Phenylalanine aqueous solutions were irradiated with Bragg-Peak energy protons (0.6–5.6 MeV). An innovative circulation setup has been developed for the irradiation of these solutions with low-energy protons. Every detectable radiolysis product has been identified and quantified after irradiation. While tyrosines are well-known products of phenylal...

Ultra-thin layers (<20 nm) of pBR322 plasmid DNA were deposited onto 2.5 µm thick polyester films and exposed to proton Bragg-peak energies (90-3000 keV) at various fluences. A quantitative analysis of the DNA damage radio-induction is here reported in terms of single and double strand breaks (SSB and DSB, respectively). The corresponding yields as...

A first attempt is made to mix the radial dose model with a simple radio-oxidation kinetic to simulate the G values for carbonate ester bond scissions in PADC induced by different ions from proton to Xenon with LET ranging from 10 to 1.20 × 104 keV μm−1. Even if some discrepancies exist between experimental and simulated data, this work tends to pr...

Radical migration between aliphatic amino acid side chains can occur in solution and intramolecularly in peptides. The kinetic constant of the hydrogen transfer reaction was measured by using competition kinetics, and the half-life as well as the distance that a radical can move within a protein was calculated.

Biocatalysts and chemical catalysts are on many aspects complementary: range of substrates and reactions, operating conditions or enantioselectivity. Nowadays, the ecological factor has to be taken into account, which urges industrials to develop "green chemistry" procedures. To deserve the "green chemistry" label, an industrial production must not...

The infrared spectra of water confined in well controlled pore glasses were recorded as a function of the pore size ranging from 8 to 320 nm and in the 30-4000 cm(-1) spectral range using the ATR technique. The experiments prove that even in the large pores, the water network is significantly perturbed. The energy of the connectivity (or hindered t...

A new footprinting method for mapping protein interactions has been developed, using tritium as a radioactive label. As residues involved in an interaction are less labeled when the complex is formed, they can be identified via comparison of the tritium incorporation of each residue of the bound protein with that of the unbound one. Application of...

In the general context of green chemistry, a considerable research effort is devoted to the elaboration of new artificial metalloproteins that catalyze, under mild conditions, the oxidation of a wide range of organic compounds, using cheap and environmentally friendly oxidants. A new artificial hemoprotein was obtained by the so-called “Trojan hors...

The design of artificial hemoproteins that could lead to new biocatalysts for selective oxidation reactions of organic compounds presents a huge interest especially in pharmacology, both for a better understanding of the metabolic profile of drugs and for the synthesis of enantiomerically pure molecules that could be involved in the design of drugs...

The synthesis of a new cationic iron metalloporphyrin–estradiol conjugate is reported. After a study of its association with the anti-estradiol antibody 7A3 by UV–visible spectroscopy, the influence of the antibody on the sulfoxidation of thioanisole by H2O2 catalyzed by the iron–metalloporphyrin has been investigated.

Catalytic antibodies with a metalloporphyrin cofactor or “hemoabzymes”, used as models for hemoproteins like peroxidases and cytochrome P450s, represent a promising route to catalysts tailored for selective oxidation reactions.The first strategy has been to produce anti-porphyrin antibodies, raised against various N-substituted- and meso-carboxyary...