Qiuhui Hu

Qiuhui Hu
Nanjing Agricultural University | NAU · College of Food Science and Technology

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194
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Publications

Publications (194)
Article
Energy deficit leads to quality deterioration of fresh mushrooms. To investigate the metabolic energy differences between Flammulina filiformis packaged with nanocomposite (NP) and conventional polyethylene (CP) bags, tandem mass tags (TMT) labeling combined with two-dimensional liquid chromatography-tandem mass spectrometry technique and biochemic...
Article
To elucidate the browning inhibition mechanism of nanocomposite packaging (nano-PM) Agaricus bisporus during cold storage, we analyzed the biophysical and biochemical indices, including substrates, key enzymes, and relative gene expression associated with browning. The results showed that nano-PM could maintain a higher total phenol content and low...
Article
In this study, the film assembled by caffeic acid-grafted-chitosan/polylactic acid (CA-g-CS/PLA) was used for packaging postharvest Agaricus bisporus, and its effects on postharvest quality of Agaricus bisporus by regulating membrane lipid metabolism were investigated. The results showed that compared to traditional polyethylene packaging, the cell...
Article
It is of great significance to develop safe and efficient dietary selenium sources to improve lead toxicity. In this study, selenate, selenite, SeMet and Se-yeast were supplied to investigate the Se biofortification and bioaccessibility in Pleurotus eryngii. The effects of Se-enriched P. eryngii on lead binding bacteria were investigated via in vit...
Article
Full-text available
In this study, modified headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS) and electronic nose (E-nose) were utilized to investigate the dynamic aroma changes of Lentinula edodes (L. edodes) at different stages of vacuum freeze drying (VFD). The extraction efficiency of volatile compounds from...
Article
In this study, a new type of packaging film assembled by caffeic acid-grafted-chitosan/polylactic acid (CA-g-CS/PLA) was prepared, and its characteristics, migration properties and effect on the postharvest quality of Agaricus bisporus, including physicochemical characteristics and antioxidant capacity, were analysed and evaluated. The results show...
Article
Hericium erinaceus, an edible and medicinal mushroom, has been utilized since ancient China due to its delicious taste and health benefits. The polysaccharide from H. erinaceus (HEP) is supposed to be one of the major bioactive compounds, which garnered widespread attention in seeking innovative applications for precision nutrition. Both in vitro a...
Article
Food 3D printing targets special consumers, such as children, elderly individuals and patients. For food 3D printing, soy protein isolate hydrogels are widely used but have obstacles in hydrogel reshaping. To improve the physical properties of the hydrogel for 3D printing, soy protein isolate can form composites with other hydrocolloids, such as Fl...
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Full-text available
The objective of this study was to develop and validate a quick and easy method for the quantification of arsenic (As), cadmium (Cd), nickel (Ni), lead (Pb), tin (Sn), and zinc (Zn) by high definition X-ray fluorescence (HDXRF) spectrometry in scallops. The optimal moisture content (less than 7.5%), particle size (0.180 mm), sample weight (0.70 g),...
Article
Quality deterioration of dried vegetables often occurs during storage, and there is limited information about the quality and microbiota of dried cabbages during storage. In the present study, the appearance, physical and flavor properties, water status and mobility, and bacterial composition of dried cabbages stored at different water activity (aw...
Article
Agaricus bisporus is prone to deteriorate after harvest. In this study, polyethylene-based packaging material loaded with nano-Ag/TiO2 (Nano-PM) was used for the preservation of A. bisporus, and compared with the polyethylene packaging material. The samples were stored at 4 ± 1 ℃ and a relative humidity of 90 % for ten days. Membrane lipid metaboli...
Article
To expound the molecular mechanism of nanocomposite packaging material (Nano-PM) in delaying quality deterioration of Flammulina velutipes during storage, transcriptome analysis was conducted to observe the changes of gene expression in F. velutipes that was packaged in Nano-PM, polyethylene packaging material (Normal-PM) and no packaging material...
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Full-text available
The controllable ultrasonic modification was hindered due to the uncertainty of the relationship between ultrasonic parameters and polysaccharide quality. In this study, the ultrasonic degradation process was established with kinetics. The physicochemical properties and prebiotic activity of ultrasonic degraded Flammulina velutipes polysaccharides...
Article
Background The high yield of ultrasonic extraction was widely studied. However, the effects of ultrasound on the properties of products were generally ignored. In this study, the structural characteristics, rheological properties, and thermal stability of Flammulina velutipes polysaccharides (FVPs) under different ultrasonic power (200, 600, 1000 W...
Article
An optical detection platform based on laser induced spectroscopy and double integrating spheres techniques was developed to obtain absorption (μa), reduced scattering coefficients (μ's) and fluorescence intensity of oil. The validation experiment carried on liquid phantoms showed that the developed system could achieve high linearity, and the resu...
Article
Pleurotus eryngi is a valuable new edible mushroom variety cultivated on a large scale in China. The polysaccharides found in this mushroom are strong bioactive. This study used simulated digestion and fermentation model to study the digestion and fermentation characteristics of Pleurotus eryngii polysaccharide (PEP) and its effect on gut microbiot...
Article
Natural biological macromolecules with putative functions of gut microbiota regulation possess the advantage of improving metabolic syndrome (MS). In this research, we aimed to determine the effects of Flammulina velutipes polysaccharide (FVP) (Expt. 1) and fecal microbiota transplantation (FMT) (Expt. 2) on MS-related disorders, gut microbiota str...
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Flammulina velutipes has anti-inflammatory, immunomodulatory, antioxidant and many bioactive properties with high contents of carbohydrate, proteins and fibers. In this study, a novel proteoglycan with polysaccharide complexes and protein chain, named PGD1-1, was isolated from F. velutipes. The structural characteristics of PGD1-1 were then determi...
Article
This study focused on exploring the structural variations of starch co-fermented by Lactobacillus plantarum LB-1 and yeast (Saccharomyces cerevisiae), and the relationship between fermented starch structure and dough characteristics. Co-fermentation resulted in the increased short chain content and crystallinity (32.07%) of starch with lower molecu...
Article
Auricularia polytricha possesses hypolipidemic effects and decreases lipid accumulation, leading to potential food additives for functional food processing. In this research, we explored the potential effects of A. polytricha noodles on hyperlipidemia and gut microbiota dysbiosis, and elucidated their possible regulatory mechanisms on lipid metabol...
Article
Background: Gut microbiota plays an indispensable role in the treatment of inflammatory bowel disease (IBD) and colitis-associated cancer (CAC). As traditional medicinal fungi, previous studies have shown that Ophiocordyceps sinensis could better maintain intestinal health via promoting the growth of probiotics in vitro compared with Cordyceps mili...
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Full-text available
The Aflatoxin B1 (AFB1) contamination in peanut oil is harmful and widespread. In this study, a laser induced fluorescence spectroscopy (LIF) system was developed for rapid and non-invasive screening of four varieties of peanut oils contaminated with different levels of AFB1. Discriminant analysis was conducted to classify the four varieties of pea...
Article
In order to clarify the effect of nanocomposite-based packaging (NP) on umami and microflora characteristics of F. filiformis during cold storage, the contents of umami amino acids and 5'-nucleotides, equivalent umami concentration (EUC), and microflora succession were investigated. Results showed that NP could delay the degradation of umami compon...
Article
The aim of the present study was to determine the inhibitory effects and the potential underlying mechanisms of a novel Pleurotus eryngii β-type glycosidic polysaccharide (WPEP) on colitis. To achieve this, sixty CD-1 (ICR) mice were divided into six groups including healthy and colitic mice treated with or without WPEP at two different doses (n =...
Article
Seven enzyme groups were applied to hydrolyze broken fruiting bodies of morel mushroom (Morchella sextelata) to extract umami substances. Physical-chemical properties, as well as compositions and concentrations of quintessential umami compounds of morel hydrolysates were analyzed. Electronic tongue and electronic nose were used to evaluate the sens...
Article
Edible mushrooms have been an important part of the human diet for thousands of years, and over 100 varieties have been cultivated for their potential human health benefits. In recent years, edible mushroom polysaccharides (EMPs) have been studied for their activities against obesity, inflammatory bowel disease (IBD), and cancer. Particularly, accu...
Article
Biologically active peptides released by proteins are important in regulating immunity. The purpose of this study was to isolate and purify an immunologically active peptide from Hericium erinaceus (H. erinaceus) and to explore its effect on cytokine secretion and differentiation of macrophages. An active peptide with an amino acid sequence, Lys-Se...
Article
Soy protein isolate (SPI) is a nutritional commercial product, while the poor solubility and gelling restricts its applications for functional foods. To surmount the challenge presented by this poor solubility, the gelling polysaccharide shows potential in ameliorating SPI. In this study, SPI/ Flammulina velutipes polysaccharide (FVP) hydrogels wer...
Article
New insight was systematically elucidated on variations of overall flavor characteristics in shiitake mushrooms (Lentinula edodes) affected by different drying techniques (hot air-drying (HD), vacuum freeze-drying (VFD), and VFD combined with HD (VFD-HD)). The volatile fingerprints of shiitake mushrooms were successfully established by headspace-ga...
Article
Previous studies have revealed that Selenium-enriched rice protein hydrolysates (SPHs) could alleviate Pb²⁺-induced apoptosis in RAW264.7 macrophages. The purpose of the current study was to detect the effect of different selenium (Se) species on immunotoxicity of the Pb²⁺-induced RAW264.7 macrophages and explore the potential immunomodulatory mech...
Article
Chitosan-pectin gel beads (CPBs) were synthesized via a facile and green method and applied to remove heavy metals from aqueous solution. The structural characteristics of CPBs were investigated by SEM and FTIR, the mechanical strength of CPBs was measured by Texture Analyzer and the stability of CPBs was evaluated in acidic solution. To study the...
Article
Functional components including β-glucan, dietary fiber, resistant starch and polyphenols extracted from various coarse cereals have been reported to prevent high-fat diet (HFD) induced obesity via modulating gut microbiota. In this study, millet, maize, oat, soybean, and purple potato were ultrafine comminuted, mixed, and then extruded for the pre...
Article
Alkylresorcinols (ARs), which are phenolic lipids found in wheat bran, have attracted a considerable amount of attention because of their antioxidant properties. However, the mechanism of ARs for regulating neuroprotective activity remains unclear. The correlation between the cellular neuroprotective effects of ARs in HT22 cells and their in vitro...
Article
5-n-Alkylresorcinols (ARs) are abundant in wheat bran and potentially antioxidative, although the neuroprotective mechanism is not fully understood. The neuroprotective effect of wheat bran ARs on H2O2-induced neuronal cells and the relationship between neuroprotection and the nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant-response...
Article
Bakery products made by cereal and edible fungi powder have a unique flavor and health benefits, dramatically enhancing the nutritional value of the products. In this study, we investigated the antifatigue effect of a novel Tricholoma matsutake cookie (TMC) by the exhaustive swimming test. Male Kunming ICR mice were randomly divided into seven grou...
Article
Fresh Flammulina filiformis is perishable and undergoes dynamic postharvest changes in physiological metabolism and biochemistry. In this study, we use label-free quantitative proteomics to elucidate the mechanisms of F. filiformis responses to cold storage and internal packaging stimuli. External quality including weight, browning, stipe elongatio...
Article
BACKGROUND Waxy maize (Zea mays L. sinensis Kulesh) is a good material for brewing. Waxy maize wine, a kind of Chinese rice wine, is strongly affected by a fermentation starter named Qu. In this study, an innovative mixed Qu, consisting of two yeasts and three molds, was produced and the raw‐starch brewing method was applied in winemaking. Three ot...
Article
Full-text available
This study aimed at isolating and identifying antioxidative peptides from Agaricus bisporus protein hydrolysate ultrafiltration fraction. Pancreatin was used to hydrolyze A. bisporus protein isolate and ultrafiltration was used to obtain 1–3 kDa fraction. Through consecutive chromatographic purification, two antioxidative fractions (C and D) were i...
Article
Background: Combination drying is recognized as an energy-efficient method utilized for dry product processing, and proper order of combination is a critical factor determining the effectiveness of the technique. In this study, hot air-drying (HD), vacuum freeze-drying (VFD), and combination drying with different orders (HD-VFD and VFD-HD) are per...
Article
The anti‐inflammatory effects of two newly identified Pleurotus eryngii polysaccharides (WPEP, NPEP) were determined in lipopolysaccharide (LPS)‐stimulated RAW264.7 macrophages in this study. Characterization analysis revealed that molecular weights of WPEP and NPEP were 167 and 274 kDa, and were mainly composed of glucose with β‐type glycosidic li...
Article
Various traditional edible fungi were demonstrated to modulate gut microbiota. In this study, ¹H NMR and high-throughput sequencing were used to investigate the difference of microecology modulating function between Ophicordyceps sinensis and Cordyceps militaris. The data showed that total 7 constituents (glucose, mannitol, trehalose, aspartate, gl...
Article
Lead (Pb), a well‐known toxic metal, has a potential of adverse effects on human health. Selenium (Se) can reduce the toxicity caused by heavy metals. In this study, we investigated the protection mechanism of the purified Se‐enriched rice protein hydrolysates (SPHs‐2) on the Pb2+‐induced cytotoxicity. A Caco‐2 cell model was established to charact...
Article
For the Flammulina velutipes polysaccharide (FVP) extracted from previous study, the present object investigated the improvement of this starch-free β-type glycosidic polysaccharide in alleviating the dextran sodium sulfate–induced ulcerative colitis (UC) mice. The absolute and relative abundance of intestinal microbes in mice feces were both deter...
Article
PEP-1b is a novel immunoregulatory protein isolated from Pleurotus eryngii, a popular edible mushroom. In this study, iTRAQ approach and bioinformatics analysis were utilized to characterize the PEP 1b-induced proteome alterations in Raw 264.7 macrophage cells, so as to comprehensively excavate the molecular mechanisms involved in the immunoregulat...
Article
Soluble solid content (SSC) is a critical index to evaluate the nutrition and flavor quality of food products. This study presents a novel strategy to predict the SSC in Agaricus bisporus slices during ultrasound-assisted osmotic dehydration (UOD). The spectral signatures of Agaricus bisporus were captured via a hyperspectral imaging (HSI) system a...
Article
Rice protein hydrolysates (RPH) is incapable of film formation by self-crosslinking due to low molecular mass. Hence, we used chitosan (CS) as a modifier and developed rice protein hydrolysates/chitosan (RPH/CS) edible composite films by means of ultrasound. Results showed that ultrasound treatment decreased the particle size and the viscosity of f...
Article
Glutenin macropolymer (GMP) plays a pivotal role in improving dough quality. In this study, a novel Lactobacillus plantarum LB-1 (LB-1) on the fermentation characteristics of dough were investigated from the perspective of GMP. The results showed that the ordered secondary structure (α-helices and β-sheets) content of GMP in dough synergistically f...
Article
Background: Fungal contamination in food products leads to mustiness, biochemical changes, and undesirable odor, which results in lower food quality and market value. In order to develop a rapid method for fungi detection, hyperspectral imaging (HSI) was applied to identify five fungi inoculated on plates (Aspergillus niger, Aspergillus flavus, Pe...
Article
The concentrations of heavy metals, such as chromium (Cr), arsenic (As), cadmium (Cd), mercury (Hg), and lead (Pb), in different muscles and edible offal of consumed pork from the city of Nanjing and related health risks were evaluated. The results showed that the detected Hg concentrations from 6 of 80 samples exceeded the maximum allowable concen...
Article
Full-text available
Flammulina velutipes contains bioactive polysaccharides that can activate the immune system. Here we report the functional characterization in an immunomodulatory F. velutipes polysaccharide (FVP) both in vitro and in vivo. In cell experiment, FVP was discovered to be an activator to induce the M1-polarization of the RAW 264.7 cells. In animal expe...
Article
Excessive exercise-induced fatigue has become a serious health issue and can cause gut dysbiosis. Tuber indicum (T. indicum) polysaccharide can act as prebiotics to regulate gut microbiota and this effect may play an important role in relieving fatigue. Therefore, T. indicum polysaccharide was prepared and mainly consisted of glucose linked with β-...
Article
In this study, chitosan (CS) was grafted with phenolic acids including gallic acid (GA-g-CS) and caffeic acid (CA-g-CS) through carbodiimide coupling. The conjugates were further developed into films by casting and were characterized in terms of appearance, structure, and mechanical and barrier properties, as well as antioxidant and antimicrobial a...
Article
Background: Water absorption is the dominant factor affecting quality deterioration of dried Lentinus edodes. Hence, we analyzed the effect of moisture content and dynamic water status on physical properties of the mushroom stored at water activity (aw ), 0.33, 0.43, 0.67, 0.76 and 0.84 for 50 days. Moisture mobility and water status were analyzed...
Article
Immunomodulatory peptides with the sequences TSeMMM and SeMDPGQQ from selenium (Se)-enriched rice protein hydrolysates (SPHs) were identified in our previous study. We synthesized these two peptides to study whether they have neuroprotective effects on Pb2+-induced oxidative stress in mouse hippocampal HT22 cells, SPHs and a purified SPH fraction (...
Article
Polysaccharides are known as one of the most important bioactive compounds in Flammulina velutipes. However, there is no accurate and comprehensive assessment method to evaluate and authenticate F. velutipes polysaccharides (FVPs) from different sources. In this study, a multiple fingerprint analysis method including scanning electron microscopy (S...
Article
Our previous study indicated that nanocomposite packaging material (Nano-PM) containing nano-Ag, nano-TiO2, nano-SiO2 and nanoattapulgite alleviated postharvest senescence of Flammulina velutipes by regulating respiration and energy metabolism. In this study, extracellular ATP (eATP) and programmed cell death (PCD) were employed as critical factors...
Article
The aim of this study was to screen for a lactic acid bacterium (LAB) that could improve the quality of whole wheat bread (WWB) and extend its shelf life. The LABs with strong antifungal activity were screened among twenty LABs. Both sourdough properties (rheology, tensility, water mobility and gluten structure) and WWB qualities (texture, volatile...
Article
Flavor deterioration is a serious problem in dried carrots during storage and is frequently accompanied by water absorption and bacterial growth. To explore the underlying mechanism of flavor deterioration, relationship among water status, exogenous bacterial composition and flavor changes in dried carrots were analyzed at different water activitie...
Article
Full-text available
A number of 17 sulfonamides (SNs) determination in porcine tissues using two new materials including Enhanced Matrix Removal for Lipid (EMR‐L) and Oasis PRiME hydrophilic‐lipophilic balance (HLB), and the conventional liquid–liquid extraction with n‐hexane (LLE) sample preparation methods were evaluated and compared. Samples were extracted uniforml...
Article
In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transi...
Article
Four phenolic acid-grafted-chitosan conjugates were prepared with different phenolic acids including gallic acid (GLA), caffeic acid (CA), gentisate acid (GTA) and sinapic acid (SA), and their characteristics were analyzed. Furthermore, the antioxidant and antimicrobial activities of conjugates were compared to screen out the optimal conjugates. Th...
Article
The changes of flavor components during boiling in Lentinus edodes were studied by high‐performance liquid chromatography and gas chromatography‐mass spectrometry in this article. Results showed that nonvolatile flavor components in L. edodes gradually transferred into the soup during boiling. Contents of nonvolatile flavor substances in the soup i...
Article
Background: Common oil-in-water plant-based emulsions are allergenic, and unstable to environmental stress, leading to the increased consumer concern about food industry. To solve the problem of safety and instability, we studied the influence of environmental stress on the stability of emulsions containing various rice protein hydrolysates, and c...
Article
A study on the transformations of six arsenic species (arsenocholine (AsC), arsenobetaine (AsB), dimethylarsinic acid (DMA), monomethylarsonic acid (MMA), arsenite (As(III)), and arsenate (As(V))) was carried out in aqueous solution by ultrasonic treatment. The transformations of arsenic species increased with time. As the ultrasonic power or tempe...
Article
Conquering rapid post-ripeness and deterioration of Agaricus bisporus is quite challenging. We previously observed that methyl jasmonate (MeJA) pretreatment postponed the deterioration of A. bisporus, but the mechanism is unknown. Here, a non-targeted metabolomics analysis by UHPLC-QTOF-MS/MS revealed that MeJA increased the synthesis of malate by...
Article
Economic loss of postharvest wheat under poor storage conditions is severe due to fungal spoilage and mycotoxin contamination. In order to study the influencing factors of the aggravation of mildew in natural wheat during storage, we assessed changes of Fusarium mycotoxins by high performance liquid chromatography, fungal communities by high-throug...
Article
Scope Pleurotus eryngii, an edible mushroom has been widely consumed as a functional food for various health benefits due to its multiple bioactive components. There are growing interests in using whole food‐based strategies to prevent colonic inflammation via potential synergistic interactions among different components within the whole foods. How...
Article
The objective was to determine the contribution of A. auricular polysaccharides (AAP) in modulating the composition and diversity of the intestinal microbe in mice. AAP was extracted from A. auricula freeze-dried powder and different amounts of AAP (40, 80, 160 mg AAP/kg body weight) were administered to 6 week-old male ICR mice by gavage feeding o...
Article
β-carotene has been often used as a hydrophobic nutrient in many functional foods owning to its excellent antioxidant activity. However, the poor orally bioavailability of β-carotene limits its utilization. To overcome such limitation, a delivery system was designed for the encapsulation of β-carotene based on oil-in-water emulsion stabilized by oa...
Article
A crude polysaccharide derived from Flammulina velutipes (FVP), mainly consisting of glucose linked with β-glycosidic bonds, was administered to 6 week-old male ICR mice by gavage for five weeks, to investigate the effects on mice intestinal microbiota and immune response. The pH of the intestinal compartments decreased while short chain fatty acid...
Article
Button mushroom (Agaricus bisporus) slices were dehydrated using ultrasound-assisted osmotic dehydration (UOD), and three osmotic agents including sucrose, glucose and sodium chloride were investigated for their effects on the mass transfer characteristics, average density and microstructure. Different mathematical models were selected to describe...
Article
Energy metabolism is accompanied with postharvest senescence, deterioration and physiological disorder in Flammulina velutipes. In this study, nanocomposite packaging material (Nano-PM) inhibited rapid respiration thus preventing accumulation of reactive oxygen species (ROS). In comparison to the normal packaging material (Normal-PM) and no packagi...
Article
Full-text available
Moisture absorption and bacterial growth are critical factors for quality deterioration of freeze-dried Agaricus bisporus. In order to explore the bacterial composition and the resultant odor changes in freeze-dried A. bisporus during storage under three typical conditions (RT: 25°C, 55% RH; HT: 37°C, 85% RH; AT: ambient temperature), bacterial div...
Article
Rapid deterioration is a serious problem in postharvest storage of Agaricus bisporus (A. bisporus). Hence, measures to improve shelf-life of the mushroom are of great importance. This study analyzed the effect of preharvest treatment of A. bisporus with methyl jasmonate (MeJA) on the postharvest storage quality. Appearance and texture characteristi...