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Citations since 2017
20 Research Items
I'm a postdoc in the Universitätsmedizin Greifswald, Germany. Currently I'm working on the oncology of acute myeloid leukemia (AML), mutations, and drug effect using multi-omics methods, i.e. RNA-seq, ATAC-seq, ChIP-seq, CUT&RUN, Hi-C.
September 2019 - December 2021
The intestine has many types of cells that are present mostly in the epithelium and lamina propria. The importance of the intestinal cells for the mammalian mucosal immune system is well-established. However, there is no in-depth information about many of the intestinal cells in teleosts. In our previous study, we reported that adherent intestinal...
Supplementary Figure 3 | Heatmap showing muscularis macrophage-related genes that were upregulated in adherent cells from the distal intestine of Atlantic salmon. AIC: adherent cells from the distal intestine; AKC: adherent cells from the head kidney; p2rx5: P2X purinoceptor 5-like; 5ntc: cytosolic purine 5-nucleotidase; p2rx4: P2X purinoceptor 4-l...
Supplementary Figure 2 | Identification of macrophage-like cells in the AIC and AKC populations using imaging flow cytometry. Macrophage-like cells were identified based on the morphologies of cells and shapes of the nuclei. The areas of macrophage-like cells in AIC (A) and AKC (B) were gated in a bright field (BF) area (cell size) vs. side scatter...
Supplementary Figure 1 | Dispersion estimates and minus over average expression of the mRNA-Seq (A, B) and small RNA-seq (C, D) dataset, respectively.
Supplementary Table 1. Details of raw, clean and mapped reads in microRNA dataset. Supplementary Table 2. Top thirty macrophage-related genes that are equally abundant in adherent cells from distal intestine and head kidney. Supplementary Table 3. Top thirty equally abundant macrophage-related miRNAs in adherent cells from distal intestine and head...
Our understanding of how individual mutations, whether present in all or just a fraction of the leukemia cells, affect cellular responses to therapy is limited. Leukemia mouse models provide a unique possibility to explore how therapy affects the evolution of genetically distinct clones and identify mechanisms of resistance allowing transfer to hum...
Our knowledge of the fish intestinal immune system, especially the phagocytic cells, is rather limited compared to those of mammals. Hence, we have studied the adherent cells isolated from the intestine of Atlantic salmon vis-à-vis those of the head kidney cells, employing flow cytometry and transcriptomics. Adherent cells from the distal intestine...
Our knowledge of the intestinal immune system of fish is rather limited compared to mammals. Very little is known about the immune cells including the phagocytic cells in fish intestine. Hence, employing imaging flow cytometry and RNA sequencing, we studied adherent cells isolated from healthy Atlantic salmon. Phagocytic activity and selected gene...
Fungi, particularly yeasts, are known essential components of the host microbiota but their functional relevance in the development of immunity and physiological processes of fish remains to be elucidated. In this study, we used a transcriptomic approach and a germ-free (GF) fish model to determine the response of newly hatched zebrafish larvae aft...
Over the last decade, the use of plant-based proteins in aquafeeds has largely increased, currently amounting up to 70% in salmon feeds. With this transition from fish meal to green diets, fish may be exposed to anti-nutritional factors, mycotoxins and other plant-associated contaminants leading to new challenges for fish health and welfare, as wel...
In many fish species, the immune system is significantly constrained by water temperature. In spite of its critical importance in protecting the host against pathogens, little is known about the influence of embryonic incubation temperature on the innate immunity of fish larvae. Zebrafish (Danio rerio) embryos were incubated at 24, 28 or 32 °C unti...
Rice tillering is a multigenic trait that influences grain yield, but its regulation molecular module is poorly understood. Here we report that OsMADS57 interacts with OsTB1 (TEOSINTE BRANCHED1) and targets D14 (Dwarf14) to control the outgrowth of axillary buds in rice. An activation-tagged mutant osmads57-1 and OsMADS57-overexpression lines showe...
Supplementary Figures S1-S9, Supplementary Tables S1-S5, Supplementary Methods and Supplementary References
Abscisic acid (ABA) regulates many aspects of plant development, including seed dormancy and germination, root growth and stomatal closure. Plant SKP1 proteins are subunits of the SCF complex E3 ligases, which regulate several phytohormone signalling pathways through protein degradation. However, little is known about SKP1 proteins participating in...
I have used LPS (SIGMA L9143) for infecting zebrafish larvae, to get the LD50 concentration, but the result is quite unstable.
Usually I prepare 4-5 mg/ml LPS (dissolved in ddH2O) for storing under -20 C, and dilute it every time I use. But, the result varies quite a lot in pre-experiments, for example, I got the suitable con. of 10 ug/ml in the first experiment, then it's 40 ug/ml in the second, now even 50 ug/ml doesn't work.
I used 50 ml plastic tube to keep LPS under -20 C, and thaw part of it just enough for each time use in the following work. I'm not sure whether these operating details are correct or not.
I really don't know what is the problem, could anyone give me some help? Thanks a lot!!