Qingshuo Zhang

Qingshuo Zhang
Oregon Health and Science University | OHSU · Department of Pediatrics

PhD

About

38
Publications
2,792
Reads
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1,026
Citations
Additional affiliations
August 2009 - present
Oregon Health & Science University
Position
  • Researcher
August 2007 - July 2009
Oregon Health & Science University
Position
  • Research Assistant
August 2005 - July 2007
Oregon Health & Science University
Position
  • PostDoc Position
Education
September 1994 - June 1999
Shanghai Institutes for Biological Sciences
Field of study
  • Molecular Biology
September 1990 - June 1994
Sichuan University
Field of study
  • Biochemistry

Publications

Publications (38)
Article
The efficiency of gene repair by homologous recombination in the liver is enhanced by CRISP/Cas9 incision near the mutation. Here we explored interventions designed to further enhance in vivo hepatocyte gene repair in a model of hereditary tyrosinemia. A two-AAV system was employed: one virus carried a SpCas9 expression cassette and the other harbo...
Article
Stem cell therapy is the administration of stem cells to a patient to treat or prevent a disease. Since stem cells possess the long-term self-renewal capacity and provide daughter cells that differentiate into the specialized cells of each tissue, stem cell therapy will theoretically improve the disease condition for the lifetime of the patient. As...
Article
Fanconi anemia (FA) is an inherited bone marrow failure disorder associated with a high incidence of leukemia and solid tumors. Bone marrow transplantation is currently the only curative therapy for the hematopoietic complications of this disorder. However, long-term morbidity and mortality remain very high, and new therapeutics are badly needed. H...
Article
Full-text available
Fanconi anemia is a genetic bone marrow failure syndrome. The current treatment options are suboptimal and do not prevent the eventual onset of aplastic anemia requiring bone marrow transplantation. We previously showed that resveratrol, an antioxidant and an activator of the protein deacetylase Sirt1, enhanced hematopoiesis in Fancd2 mutant mice a...
Article
Androgens are widely used for treating Fanconi anemia and other human bone marrow failure syndromes, but their mode of action remains incompletely understood. Aged Fancd2-/- mice were used to assess the therapeutic efficacy of oxymetholone and its mechanism of action. 18-month old Fancd2-/- mice recapitulated key human Fanconi anemia phenotypes inc...
Article
s: AACR Special Conference: Cancer Susceptibility and Cancer Susceptibility Syndromes; January 29-February 1, 2014; San Diego, CA Fanconi's anemia (FA) is a genetically heterogeneous inherited disorder characterized by bone marrow failure, birth defects and cancer susceptibility. At the cellular level, FA features hypersensitivity to DNA cross-lin...
Article
Full-text available
Androgens are widely used for treating Fanconi anemia (FA) and other human bone marrow failure syndromes, but their mode of action remains incompletely understood. Aged Fancd2(-/-) mice were used to assess the therapeutic efficacy of oxymetholone (OXM) and its mechanism of action. Eighteen-month-old Fancd2(-/-) mice recapitulated key human FA pheno...
Article
Fanconi anemia (FA) patients suffer from progressive bone marrow failure and often develop cancers. Previous studies showed that antioxidants tempol and resveratrol (RV) delayed tumor onset and reduced hematologic defects in FA murine models, respectively. Here we tested whether antioxidants N-acetylcysteine (NAC) or RV could delay cancer in tumor...
Data
Full-text available
Severe sepsis remains a poorly understood systemic inflammatory condition with high mortality rates and limited therapeutic options in addition to organ support measures. Here we show that the clinically approved group of anthracyclines acts therapeutically at a low dose regimen to confer robust protection against severe sepsis in mice. This saluta...
Article
Severe sepsis remains a poorly understood systemic inflammatory condition with high mortality rates and limited therapeutic options in addition to organ support measures. Here we show that the clinically approved group of anthracyclines acts therapeutically at a low dose regimen to confer robust protection against severe sepsis in mice. This saluta...
Article
Fanconi anemia patients suffer from progressive bone marrow failure. An overactive p53 response to DNA damage contributes to the progressive elimination of Fanconi anemia hematopoietic stem and progenitor cells (HSPC), and hence presents a potential target for therapeutic intervention. To investigate whether the cell cycle regulatory protein p21 is...
Article
SCI-1 Fanconi anemia (FA) is a genetically heterogeneous inherited disorder characterized by bone marrow failure, birth defects, and cancer susceptibility. At the cellular level, FA features hypersensitivity to DNA cross-linking agents, cell cycle abnormalities, and abnormal responses to cytokines. Therapy for the hematopoietic defects of FA has co...
Article
Fanconi anemia (FA) is an inherited DNA repair deficiency syndrome. FA patients undergo progressive bone marrow failure (BMF) during childhood, which frequently requires allogeneic hematopoietic stem cell transplantation. The pathogenesis of this BMF has been elusive to date. Here we found that FA patients exhibit a profound defect in hematopoietic...
Article
Full-text available
Progressive bone marrow failure is a major cause of morbidity and mortality in human Fanconi Anemia patients. In an effort to develop a Fanconi Anemia murine model to study bone marrow failure, we found that Fancd2(-/-) mice have readily measurable hematopoietic defects. Fancd2 deficiency was associated with a significant decline in the size of the...
Article
Full-text available
Background: Retinitis pigmentosa (RP) is characterized by progressive night blindness, visual field loss, altered vascular permeability and loss of central vision. Currently there is no effective treatment available except gene replacement therapy has shown promise in a few patients with specific gene defects. There is an urgent need to develop th...
Article
Full-text available
Fanconi anemia (FA) is a genetic disorder characterized by congenital abnormalities, bone marrow failure, and marked cancer susceptibility. FA patients have an elevated risk of developing hematologic malignancies and solid tumors. Using Fancd2(-/-) knockout mice as a model of FA, we examined the potential of tempol, a nitroxide antioxidant and a su...
Article
Full-text available
Drosophila Pumilio (Pum) protein is a translational regulator involved in embryonic patterning and germline development. Recent findings demonstrate that Pum also plays an important role in the nervous system, both at the neuromuscular junction (NMJ) and in long-term memory formation. In neurons, Pum appears to play a role in homeostatic control of...
Data
Supplementary Material (0.05 MB DOC)
Data
Correlation Between Matrix Prediction Scores and Pum-Binding Affinities. The abscissa is the measured percentage binding of Pum to the mRNA target. The ordinate is the prediction score, which is the maximum matrix score of all the sites in a sequence. The 12 data points represent 12 mRNA sequences (nine test sequences in Table 1 and three control s...
Data
Estimation of False Positives with Random Shuffle Tests on the 151 Synaptic Genes. Shuffling times n = 500. (A) Matrix NRE_M10. (B) Matrix MmSelex_M8. The gray bars represent the hits with the original matrix. The black bars represent the average hits with randomly shuffled matrices. The error bar is the standard deviation across the 500 shuffling...
Data
NRE_PAT Predictions (0.02 MB XLS)
Data
NRE_M10 Predictions (0.02 MB XLS)
Data
Segmentation Pattern in Embryos of Modified Hunchback Gene Transformant Lines (0.02 MB XLS)
Data
Synaptic Gene List (0.04 MB XLS)
Data
MmSelex_M8 Matrix and the Correlation of Its Prediction Scores to Pum-Binding Affinities. (A) Base-frequency matrices obtained using Gibbs Sampler with mouse SELEX sequence data from White et al. [41]. Position 5 is a motif gap as in Gibbs output, which means that the base in this position is irrelevant. DNA notation is used as in Figure 2. (B) Cor...
Data
dlg1 Gene Structure as Shown in the FlyBase Genome Browser. Transcript dlg1-RA and dlg1-RC are located on non-overlapping regions on the fly genome. (0.06 MB TIF)
Data
Comparison of the Overlap of Our Pum Target Predictions with the Adult Specific Targets from Gerber et al. [15] in the Synaptic Gene Set. Pred+ and Pred− represent the number of our positive or negative prediction, respectively. PD+ and PD− represent the number of positive or negative pulled-down targets from Gerber et al. (2006), respectively. (0....
Data
NRE_M8 Predictions (0.03 MB XLS)
Article
Full-text available
Telomerase is a ribonucleoprotein enzyme complex that reverse-transcribes an integral RNA template to add short DNA repeats to the 3'-ends of telomeres. G-quadruplex structure in a DNA substrate can block its extension by telomerase. We have found that hnRNP A1--which was previously implicated in telomere length regulation--binds to both single-str...
Article
Previous investigations show that tRNA(Arg)-induced conformational changes of arginyl-tRNA synthetase (ArgRS) Omega-loop region (Escherichia coli (E. coli), Ala451-Ala457) may contribute to the productive conformation of the enzyme catalytic core, and E. coli tRNA(2)(Arg)(ICG)-bound and -free conformations of the Omega-loop exchange at an intermedi...
Article
The 19F nuclear magnetic resonance (NMR) spectra of 4-fluorotryptophan (4-F-Trp)-labeled Escherichia coli arginyl-tRNA synthetase (ArgRS) show that there are distinct conformational changes in the catalytic core and tRNA anticodon stem and loop-binding domain of the enzyme, when arginine and tRNA(Arg) are added to the unliganded enzyme. We have ass...
Article
RNA-protein interactions play crucial roles in many fundamental cellular processes. Recently studies on RNA-protein interactions are getting considerable progress with the improvement of techniques and the appearance of new methods. Many protein-binding sites of RNAs have been characterized, some RNA-binding domains of proteins have been found, and...
Article
Escherichia coli 4-fluorotryptophan-substituted arginyl-tRNA synthetase was biosynthetically prepared and purified from a tryptophan auxotroph which could overproduce this enzyme. A method was developed to separate 4-fluorotryptophan from tryptophan and to determine accurately their contents in the 4-fluorotryptophan-containing proteins. It was con...
Article
The effect of N-bromosuccinimide (NBS) on the activity of Escherichia coli arginyl-tRNA synthetase (ArgRS) was studied. The results showed that only one tryptophan residue was easy of access to the reagent and was closely related to enzyme activity. When all the five tryptophan residues in ArgRS were changed via site-directed mutagenesis singly int...

Projects

Project (1)
Project
My main research interest is in autoimmune aspects of retinal disorders, including autoimmune retinopathies, uveitis, optic neuritis, and autoimmune neuropathies. We study the role of autoantibodies in cancer-associated retinopathy (CAR) and melnoma-associated retinopathy.