Qian Wang

Qian Wang
Montana State University | MSU · Department of Microbiology and Cell Biology

Doctor of Philosophy

About

36
Publications
5,102
Reads
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917
Citations
Citations since 2017
17 Research Items
809 Citations
2017201820192020202120222023050100150
2017201820192020202120222023050100150
2017201820192020202120222023050100150
2017201820192020202120222023050100150

Publications

Publications (36)
Article
Significance Observations summarized herein contribute to an ongoing paradigm shift in microbial ecology, documenting an emergent property of ecosystem function that further challenges the perception that biogenic methane (CH 4 ) production is strictly an anaerobic process. Relevant metabolites, a model bacterial isolate, gene, and enzyme are ident...
Article
Full-text available
The microbial ars operon encodes the primary bacterial defense response to the environmental toxicant, arsenic. An important component of this operon is the arsR gene, which encodes ArsR, a member of the family of proteins categorized as DNA-binding transcriptional repressors. As currently documented, ArsR regulates its own expression as well as ot...
Chapter
A method for measuring mRNA copies in intact bacterial cells by fluctuation localization imaging-based fluorescence in situ hybridization (fliFISH) is presented. Unlike conventional single-molecule FISH, where the presence of a transcript is determined by fluorescence intensity, fliFISH relies on On-Off duty cycles of photo-switching dyes to set a...
Article
Full-text available
Environmental toxicant exposure contributes to the morbidity and mortality of many human diseases. With respect to arsenic, microbially driven chemical transformations dictate its toxicity and mobility in virtually every environment yet studied, so a general hypothesis is that human gut microbiome determines disease outcome following exposure. Howe...
Article
Agrobacterium tumefaciens GW4 is a heterotrophic arsenite-oxidizing bacterium with a high resistance to arsenic toxicity. It is now a model organism for studying the processes of arsenic detoxification and utilization. Previously, we demonstrated that under low-phosphate conditions, arsenate [As(V)] could enhance bacterial growth and be incorporate...
Article
Full-text available
Arsenic (As) is a metalloid that occurs widely in the environment. The biological oxidation of arsenite [As(III)] to arsenate [As(V)] is considered a strategy to reduce arsenic toxicity and provide energy. In recent years, research interests in microbial As(III) oxidation have been growing, and related new achievements have been revealed. This revi...
Article
Full-text available
Aromatic compounds and arsenic (As) often coexist in the environment. As(III)-oxidizing bacteria can oxidize the more toxic As(III) into the less toxic As(V), and As(V) is easily removed. Microorganisms with the ability to degrade aromatic compounds and oxidize arsenite [As(III)] may have strong potential to remediate co-contaminated water. In this...
Article
Full-text available
A Gram-staining negative, aerobic, non-motile, rod-shaped bacterial strain, designated YS-37T, was isolated from soil in a manganese factory, People’s Republic of China. Based on16S rRNA gene sequence analysis, strain YS-37T was most closely related to Lysobacter pocheonensis Gsoil 193T (97.0%), Lysobacter dokdonensis DS-58T (96.0%) and Lysobacter...
Article
A heterotrophic arsenite [As(III)]-oxidizing bacterium Agrobacterium tumefaciens GW4 isolated from As(III)-rich groundwater sediment showed high As(III) resistance and could oxidize As(III) to As(V). The As(III) oxidation could generate energy and enhance growth, and AioR was the regulator for As(III) oxidase. To determine the related metabolic pat...
Article
Full-text available
The "CH4 oversaturation paradox" has been observed in oxygen-rich marine and lake waters, and viewed to significantly contribute to biosphere cycling of methane, a potent greenhouse gas. Our study focused on the intriguing well-defined pelagic methane enriched zone (PMEZ) in freshwater lakes. Spiking Yellowstone Lake PMEZ samples with (13) C-labele...
Article
Full-text available
Some arsenite [As(III)]-oxidizing bacteria exhibit positive chemotaxis towards As(III), however, the related As(III) chemoreceptor and regulatory mechanism remain unknown. The As(III)-oxidizing bacterium Agrobacterium tumefaciens GW4 displays positive chemotaxis towards 0.5–2 mM As(III). Genomic analyses revealed a putative chemoreceptor-encoding g...
Article
Full-text available
Antimonite [Sb(III)]-oxidizing bacteria can transform the toxic Sb(III) into the less toxic antimonate [Sb(V)]. Recently, the cytoplasmic Sb(III)-oxidase AnoA and the periplasmic arsenite [As(III)] oxidase AioAB were shown to responsible for bacterial Sb(III) oxidation, however, disruption of each gene only partially decreased Sb(III) oxidation eff...
Article
Full-text available
Previously, we found that arsenite (AsIII) oxidation could improve the generation of ATP/NADH to support the growth of Agrobacterium tumefaciens GW4. In this study, we found that aioE is induced by AsIII and located in the arsenic island near the AsIII oxidase genes aioBA and co-transcripted with the arsenic resistant genes arsR1-arsC1-arsC2-acr3-1...
Article
Full-text available
Background Microbial antimonite [Sb(III)] oxidation converts toxic Sb(III) into less toxic antimonate [Sb(V)] and plays an important role in the biogeochemical Sb cycle. Currently, little is known about the mechanisms underlying bacterial Sb(III) resistance and oxidation. ResultsIn this study, Tn5 transposon mutagenesis was conducted in the Sb(III)...
Article
Full-text available
A Gram-staining positive, strict aerobic, non-motile and rod-shaped bacterial strain, designated YS-17T, was isolated from soil in the Lengshuijiang antimony mine, Hunan province, P. R. China. Comparative 16S rRNA gene sequencing analysis clustered with the Flavihumibacter strains and strain YS-17T was most closely related to Flavihumibacter cheona...
Article
Antimony (Sb) is a toxic metalloid that occurs widely at trace concentrations in soil, aquatic systems, and the atmosphere. Nowadays, with the development of its new industrial applications and the corresponding expansion of antimony mining activities, the phenomenon of antimony pollution has become an increasingly serious concern. In recent years,...
Article
Full-text available
A Gram-negative, aerobic, non-motile, red-pigmented and rod-shaped bacterium, designated XF-6RT, was isolated from mountain soil in Sichuan province of China. Phylogenetic trees based on 16S rRNA gene analysis showed that XF-6RT belonged to the genus Hymenobacter. The highest level 16S rRNA gene sequence similarities of strain XF-6RT were Hymenobac...
Article
Full-text available
Previously, the expression of arsenite [As(III)] oxidase genes aioBA was reported to be regulated by a three-component regulatory system, AioXSR, in a number of As(III)-oxidizing bacterial strains. However, the regulation mechanism is still unknown when aioXSR genes are absent in some As(III)-oxidizing bacterial genomes, such as in Halomonas sp. HA...
Article
Antimony (Sb) and its compounds are listed by the United States Environmental Protection Agency (USEPA, 1979) and the European Union (CEC, 1976) as a priority pollutant. Microbial redox transformations are presumed to be an important part of antimony cycling in nature; however, regulation of these processes and the enzymology involved are unknown....
Article
Full-text available
Arsenic and antimony are toxic metalloids and considered priority environmental pollutants by the U.S. Environmental Protection Agency. Significant advances have been made in understanding microbe-arsenic interactions and how they influence arsenic redox speciation in the environment. However, even the most basic features of how and why a microorga...
Article
The fate of arsenate (AsV) generated by microbial arsenite (AsIII) oxidation is poorly understood. Agrobacterium tumefaciens wild type strain (GW4) was studied to determine how the cell copes with AsV generated in batch culture. GW4 grown heterotrophically with mannitol used AsIII as a supplemental energy supply as reflected by enhanced growth and...
Article
Methods to maintain postharvest quality and prolong shelf life of cucumber (Cucumis sativus L.) are sought after, because cucumber fruits are highly perishable and sensitive to both ethylene and chilling temperatures (below 10C). The authors evaluated the efficacy of ionized air treatments to delay senescence in cucumber. Fresh cucumbers were store...
Article
Full-text available
Antimony (Sb) and copper (Cu) are toxic heavy metals that are associated with a wide variety of minerals. Sb(III)-oxidizing bacteria that convert the toxic Sb(III) to the less toxic Sb(V) are potentially useful for environmental Sb bioremediation. A total of 125 culturable Sb(III)/Cu(II)-resistant bacteria from 11 different types of mining soils we...
Article
Full-text available
Agrobacterium tumefaciens strain C58 is a Gram-negative soil bacterium capable of inducing tumors (crown galls) on many dicotyledonous plants. The genome of A. tumefaciens strain C58 was re-annotated based on the Z-curve method. First, all the 'hypothetical genes' were re-identified, and 29 originally annotated 'hypothetical genes' were recognized...
Data
Identification of non-coding ORFs from annotated hypothetical genes. (DOC)
Data
The PCR results of 29 DNA fragments re-annotated as no-coding ORFs. The expected products of PCR used total DNA as template were all obtained with the right sizes, 16S rRNA gene (404 bp), recA (425 bp), NC-1 (362 bp), NC-2 (437 bp), NC-3 (468 bp), NC-4 (106 bp), NC-5 (127 bp), NC-6 (115 bp), NC-7 (210 bp), NC-8 (109 bp), NC-9 (291 bp), NC-10 (254 b...
Data
The PCR results of 19 DNA fragments re-annotated as potential protein-coding genes. The expected products of PCR used total DNA of late log phase as templates were all obtained with the right sizes, 16S rDNA (404 bp), recA (425 bp), PC-1 (322 bp), PC-2 (291 bp), PC-3 (268 bp), PC-4 (162 bp), PC-5 (456 bp), PC-6 (341 bp), PC-7 (235 bp), PC-8 (251 bp...
Data
The PCR results with RNA of 19 DNA fragments re-annotated as potential protein-coding genes. (A) The PCR with RNA of early log phase as templates. (B) The PCR with RNA of late log phase as templates. (C) The PCR with RNA of stationary phase as templates. When the total RNAs were used as templates in the PCR, no amplification band was produced. (TIF...
Data
The PCR results with total RNA of 29 DNA fragments re-annotated as no-coding ORFs. (A) The PCR with RNA of early log phase as templates. (B) The PCR with RNA of late log phase as templates. (C) The PCR with RNA of stationary phase as templates. When the total RNAs were used as templates in the PCR, no amplification band was produced. (TIF)

Questions

Question (1)
Question
I have the concentration of the oxygen in a sealed serum bottle. The sealed serum bottle contains 20 mL liquid medium and 50 mL gas headspace. How can I calculate the dissolved oxygen concentration in the liquid based on the oxygen concentration in headspace? The pressure is considered the same with the atm. Thanks!

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Projects

Projects (2)
Project
Methane ecology in Yellowstone lake