Pv Parvati Sai Arun

Abstract Pomegranate (Punica granatum L.) is an important fruit crop, rich in fiber, vitamins, antioxidants, minerals and source of different biologically active compounds. The bacterial blight caused by Xanthomonas axonopodispv. punicae is a serious threat to the crop leading to 60- 80 % yield loss under epiphytotic conditions. In this work, we ha...

Structured Abstract: Background: SARS-CoV-2 causes COVID19 disease where there is no suitable drugs available. The objective of the work is to repurpose the drugs prescribed for SARS-CoV as the drugs for the control of SARS-CoV-2. Method: In this work, we have used homology searches and docking methods for understanding the mechanism of the drugs...

Pomegranate (Punica granatum L.) is an important fruit crop, rich in fiber, vitamins, antioxidants, minerals and source of different biologically active compounds. The bacterial blight caused by Xanthomonas axonopodispv. punicae is a serious threat to the crop leading to 60- 80 % yield loss under epiphytotic conditions. In this work, we have genera...

Prochlorococcus marinus MIT 9303 is a marine cyanobacterium found in sea waters. It was first isolated from a depth of 100 m in the Sargasso Sea in the year 1992. This cyanobacterium serves as a good model system for scientific research due to the presence of many desirable characteristics like smaller in size, ability to perform photosynthesis and...

Tuberculosis (TB) is the deadly infectious disease challenging the public health globally and its impact is further aggravated by co-infection with HIV and the emergence of drug resistant strains of Mycobacterium tuberculosis. In this study, we attempted to characterise the Rv2004c encoded protein, a member of DosR regulon, for its role in drug res...

The present study deals with evaluation of random mutations and their impact on the production of anti-tumor drug Daunorubicin. Daunorubicin is a secondary metabolite, produced by the strain Streptomyces peucetius. In our study, S. peucetius was irradiated with UV rays to create physical mutagenesis and was also subjected to chemical mutagenesis by...

In this work we proposed a research hypothesis which mainly focuses on the role of gut microbiota, in relation with chronic kidney disease, existing in the village of Uddanam belonging to the State of Andhra Pradesh, India. Earlier studies conducted on various physical and chemical parameters such as food, water, soil, pesticides etc., could not fi...

Bengal tiger (Panthera tigris tigris), one of six extant tiger subspecies, occurs solely in the Indian subcontinent. Although endangered and threatened by various extinction risks, this is the most populous tiger subspecies with the highest genetic diversity and strongest chance of survival in the wild. Availability of high quality genomic informat...

Molecular signatures and their interactions behind the successful establishment of infection of Mycobacterium tuberculosis (Mtb) inside macrophage are largely unknown. In this work, we present an inter-system scale atlas of the gene expression signatures, their interactions and higher order gene functions of macrophage-Mtb environment at the time o...

This study focuses on the identification of proteins, which are annotated as hypothetical proteins, but possess putative arsenate reductase properties among cyanobacteria, by using bioinformatics approach. In the present work, we have chosen the protein se quence of the gene all0195 , which was annotated as hypothetical protein and later identi...

Based on the clustering coefficient applied in our earlier research paper, a total 10 proteins with high clustering coefficient were selected as the candidate proteins which involve in Type II diabetes. The downloaded PDB structures of these 10 proteins were submitted RASPD server for identification of putative drug targets. For many drug targets g...

UpCoT is a pipeline tool developed by automating the series of steps involved in prediction of cis-regulatory elements. UpCoT generates orthologs for each gene in target genome using bi-directional best blast hit against the reference genomes, then identifies potential orthologous transcriptional units using intergenic distance. Finally it generate...

Gene conservation, duplication and constitutive expression are intricately linked and strong predictors of essentiality. Here we introduce metrics based on diversity indices to measure gene conservation, duplication and constitutive expression and validate them by measuring their performance in prediction of essential genes. Conservation and duplic...

Identification of putative cis regulatory elements by comparative analysis of genomes helps us to understand mechanism of global gene expression. Many approaches were previously used for identification of cis regulatory elements. Here we use phylogenetic foot-printing analysis and MEME suite for predicting cis regulatory elements in the genome of S...

CyanoPhyChe is a user friendly database that one can browse through for physico-chemical properties, structure and biochemical pathway information of cyanobacterial proteins. We downloaded all the protein sequences from the cyanobacterial genome database for calculating the physico-chemical properties, such as molecular weight, net charge of protei...

SDS-PAGE analyses of soluble and insoluble fractions of E. coli expressing the Synechocystis sp. PCC6803 proteins. Solubility of (A) Sll0649, (B) Sll0088 and (C) Sll0359 proteins. 0.4 mM IPTG (final concentration) was added to E. coli cells for inducing expression. The cells were harvested for separation of soluble and insoluble protein fractions,...

Genome sequence of Serratia proteamaculans 568 revealed the presence of three family 33 chitin binding proteins (CBPs). The three Sp CBPs (Sp CBP21, Sp CBP28 and Sp CBP50) were heterologously expressed and purified. Sp CBP21 and Sp CBP50 showed binding preference to β-chitin, while Sp CBP28 did not bind to chitin and cellulose substrates. Both Sp C...

Ni-NTA agarose purification of Sp CBPs. Recombinant Sp CBP21, Sp CBP28 and Sp CBP50 were purified using Ni-NTA agarose column chromatography. Elution buffer containing 250 mM imidazole was used to elute Sp CBPs from the column and loaded on 12% SDS-PAGE followed by staining with Coomassie brilliant blue G-250. Lane 1: Protein standards size in kDa...

Dose-response effects for Sp CBP21 and Sp CBP50 in degradation of β-chitin. Reaction mixture containing 0.25 mg/mL of chitin substrates (α-/β-chitin), 1.0 µM Sp ChiD incubated with different concentrations of Sp CBP21/Sp CBP50 (0.05–0.40 µM) as indicated in 50 mM sodium phosphate buffer pH 7.0. After incubation at 37°C for 24 h at 1000 rpm, 100 µL...

Binding of Sp CBPs towards soluble polymeric substrates. Affinity non-denaturing gel electrophoresis was performed at 4°C by preparing 8% polyacrylamide gels. Ten micrograms of Sp CBPs and BSA were electrophoresed without (A), or with 0.1% (w/v) substrates glycol chitin (B), laminarin (C), and CM-cellulose (D). Proteins were visualized by Coomassie...