Purnima Ashok

East West Group of Institutions Bangalore · R@D

To evaluate the effectiveness of fucoidan gelatine containing chitosan injectable hydrogel in bone regeneration in rats by histopathological experimentation, a randomized laboratory experimental trial was conducted on 30 Albino Wistar rats. Animals were randomly distributed into 3 groups. Micro defects of 2-3 mm were created in the tibia of each an...

Objective: To evaluate the synergistic protective effect of Momordica charantia and Phyllanthus amarus combination (MC+PA) of doses 200 and 400 mg/kg on the liver in different experimental models of hepatotoxicity. Methods: The hepatoprotective activity was evaluated in ethanol and anti-tubercular drugs (isoniazid-INH, rifampicin-RIF) induced hepat...

Objective: To evaluate antidiabetic, hypolipidemic and antioxidant activity of polyherbalformulation (PHF) aqueous extract in streptozotocin-nicotinamide induced diabetes in rats. Methods: Fasting blood glucose, lipid profiles, serum insulin and glycosylate haemoglobin (HbA1C) were determined in normal and streptozotocin-nicotinamide induced diabet...

Objective: To evaluate antidiabetic, hypolipidemic and antioxidant activity of polyherbalformulation (PHF) aqueous extract in streptozotocin-nicotinamide induced diabetes in rats.Methods: Fasting blood glucose, lipid profiles, serum insulin and glycosylate haemoglobin (HbA1C) were determined in normal and streptozotocin-nicotinamide induced diabeti...

Phyllanthus emblica Linn. (amla) is used in Ayurveda, the ancient Indian system of medicine and its major constituent is vitamin C which has effective free radical scavenging property. The purpose of this study was to evaluate the in vitro antioxidant activity and the bioavailability profile of vitamin C in amla and its combinations with piperine a...

The study aimed to enhance the transdermal permeation of diclofenac sodium using combined strategy of constant voltage iontophoresis (CVI) and chemical penetration enhancers (CPE). Four hydrogels of diclofenac sodium were formulated with the key CPE and hydroxyethyl cellulose (HEC) as a viscosity imparting agent. In vitro permeation studies of the...

The aim of the study was to develop hydrogels and investigate the suitability for transdermal delivery of diclofenac sodium (DS) using constant voltage iontophoresis (CVI). Four batches of hydrogels of DS were developed using hydroxylethyl cellulose (HEC) as matrix material and terpenes as penetration enhancers. The hydrogels displayed a viscosity...

The purpose of this investigation was to compare the bioavailability of standard powdered coenzyme (CoQ10) with that of the CoQ10 complex. Albino Wistar rats weighing between 150180 g were divided into two groups of 16 each and received 0.42mg/kg body weight of the standard powder and CoQ10 complex respectively. On day 0 after administration of the...

ABSTRACT−Murrraya koenigii (curry leaves) and its combination with Emblica officinalis (amla) were evaluated for their antianaemic activity in aluminium chloride induced anaemic animals. Rats were divided into 7 groups of 6 each. Group 1 was given normal saline and served as control and all other groups were given 0.5 mg/kg b.w of AlCl3 for 30 days...

The present study was undertaken to support the use of herbs in folklore medicine for wound healing activity. In the present investigation, a comparative study and screening was done on two polyherbal formulations GC-01 and GC-02 fortified with different herb extracts in ghee for wound healing activity in experimentally induced wounds in rodents. W...

There is a growing interest in the screening of antihyperlipidemic activity and the present study deals with the screening of a polyherbomineral formulation (AF-LIP) which possess many important ingredients reported to have antihyperlipidemic potency. Acute antihyperlipidemic activity was evaluated by using Triton WR-1339 (100 mg kg(-1)) and chroni...

This study was conducted to evaluate the hepatoprotective effects of Hemidesmus indicus var. pubescens leaf on CCL4 induced hepatotoxicity. CCL4 was administered at a dose of 0.5 ml/kg b.w. for 7days along with the methanol extract of H. indicus var. pubescens leaves at doses 200, 400 and 600 mg/kg b.w. Administration of CCL4 caused profound hepati...

Objective: To evaluate the hepatoprotective potential of methanol extract of roots and rhizomes of Smilax zeylanica L on carbontetrachloride (CCl4) induced hepatotoxicity in Wistar albino rats. Methods: Hepatotoxicity was induced in Wistar albino rats of either sex by administration of CCl4 0.5 ml/kg p.o. once a day for 7 days. The methanol extract...

Aqueous and ethanolic extract of roots of Coscinium fenestratum and flowers of EmbeliaribesBurms. were investigated for its possible hepatoprotective property in paracetamol and carbon tetrachloride induced hepatotoxicity in laboratory animals, in view of paucity of such studies,despite proven secondary metabolites with promising antioxidant proper...

The present study envisaged to evaluate in-vitro anti oxidant activity of ethanolic and aqueous extracts of roots of Coscinium fenestratum and extracts of Embelia ribes flower, by estimating nitric oxide scavenging activity, ferric thiocynate and total reduction capacity and carrying out DPPH assay of increasing concentration of extracts and compar...

Context: Smilax zeylanica L.(Smilacaceae) is a climbing shrub with woody prickly stems. Objective: This study evaluated the hepatoprotective effect of S. zeylanica against paracetamol induced hepatotoxicity in Wistar rats. Materials and methods: The protective effects of the methanol extract (200 and 400 mg/kg) of root and rhizome of S. zeylan...

Hemidesmus indicus (L.) R. Br. var. pubescens (W. & A.) H k. f. was evaluated for hepatoprotective property against paracetamol induced hepatotoxicity in Wistar albino rats. In this study, methanol extract of the leaves of H. indicus var. pubesce-ns at doses 200 and 400 mg/kg body weight were administered p.o. along with paracetamol at the dose 1 g...

Desmodium triquetrum contains a wide variety of free radical scavenging molecules, such as phenolic and nitrogen compounds, terpenoids, and carotenoids that are rich in antioxidant activity.[1] The chloroform and alcohol extracts of D. triquetrum were reported for their antibacterial activity. Epidemiological studies have shown that many of these a...

Silymarin as a potent antioxidant has been appreciated for enhancing liver function. Inhalation of carbon tetrachloride (CCl4) vapours twice a week for 12 consecutive weeks led to liver cirrhosis in experimental rats with significant increase in the "free" and decrease in "total" activities of lysosomal enzymes, viz; N-acetyl glucosaminidase (NAG),...

Aim of the studyAlstonia scholaris (Family: Apocynaceae) is a medicinal plant which is indicated for the treatment of various diseases including arthritis in folklore medicine. The purpose of this study is to investigate the antiarthritic activity and in vivo antioxidant role of A. scholaris leaves in animal models.Materials and methodsThe ethanol...

The current study investigates the antihyperhomocysteinemic and antihyperlipidemic effect of chloroform and methanol extracts of the leaves of Trichilia connaroides in methionine-induced hyperhomocysteinemic rats. Hyperhomocysteinemia was induced in albino Wistar rats by oral administration of L-Methionine (1 gm / kg) and they were treated simultan...

The primary objective of this study was to investigate the antihyperlipidemic effect of the chloroform (CETC) and methanol (METC) extracts of leaves of Trichilia connaroides in hypercholesterolemic rats and, subsequently, to evaluate the possible mechanism of its antihyperlipidemic effect. The antihyperlipidemic activity of CETC and METC (100 mg/kg...

Carbon tetrachloride (CCl4), a major cause of chronic liver failure represents a significant clinical problem. We investigated the role of quercetin on lysosomal enzymes activity, catecholamines (adrenaline and noradrenalin) and growth hormone levels in CCl4 vapour induced liver damage in rats. CCl4 damage and its prevention by quercetin were asses...

The present study was conducted to investigate the antioxidant property of roots and rhizomes of Smilax zeylanica L. by in vitro methods. Methanol and aqueous extracts of the drug were evaluated for in vitro antioxidant activity. Methanol extract showed potential scavenging effect against DPPH, hydrogen peroxide and ABTS free radicals. HPTLC studie...

The current study envisaged evaluation of potential antioxidant role of chloroform and methanol extracts of leaves of Trichilia connaroides in hypercholesterolemia induced oxidative stress. Male Albino Wistar rats were rendered hypercholesterolemic by feeding them with high fat diet. Hypercholesterolemic animals were then treated orally, each day w...

To evaluate the potential of Mimusops elengi in the treatment of renal calculi. Petroleum ether, chloroform, and alcohol extracts of Mimusops elengi bark were evaluated for antiurolithiatic and antioxidant activity in male albino Wistar rats. Ethylene glycol (0.75%) in drinking water was fed to all the groups (Groups II-IX) except normal control (G...

In the present study petroleum ether and alcoholic extracts of Centratherum anthelminticum (L) Kuntze seed (100 mg and 200 mg/kg p.o.) were evaluated for antiinflammatory activity in acute and subacute models of inflammation. It was found that both petroleum ether and alcoholic extracts showed significant reduction in paw oedema in carrageenan-indu...

Chloroform and methanol extracts of leaves of Trichilia connaroides are reported to possess hypocholesterolemic and antihypercholesterolemic activity. The current study investigated the effect of these extracts on serum triglyceride level in animal models of acute hypertriglyceridemia, namely fructose load test and oral lipid load tolerance test, i...

In the present study, 70% ethanol extract of Mimusops elengi Linn. bark was assessed for antiinflammatory, analgesic and antipyretic activities in animals. The antiinflammatory activity of ethanol extract of Mimusops elengi (200 mg/kg, p.o) was evaluated using carrageenan-induced paw edema and cotton pellet-induced granuloma models. Analgesic effec...

In the present study, petroleum ether, chloroform, and alcoholic extracts of Mimusops elengi Linn. bark (200 mg/kg body weight, p. o.) were tested for diuretic activity. The animals were grouped into five of six animals each. The first group received only 0.9% sodium chloride solution (25 ml/kg body weight) and the second group received the standar...

In the present investigation, antidiabetic and antioxidant activities of a polyherbomineral formulation, Gly-13-C was evaluated in streptozotocin-induced diabetic male Wistar rats and compared with that of glibenclamide (600 μg kg-1), a reference drug. After induction of diabetes by a single intraperitoneal injection of streptozotocin (65 mg kg-1),...

Acute and subacute toxicity of the ethanolic extract from Calotropis gigantea R.Br. flower was investigated. In acute toxicity study oral dose of 2000 mg/kg of the ethanolic extract did not produce mortality or changes in the general behavior and gross appearance of internal organs of mice and rats. In subacute toxicity study, ethanolic extract was...

The study was intended to evaluate the anti-inflammatory activity of non-irradiated and gamma-irradiated (dose 5KGy and 10KGy) samples of Andrographis paniculata in carageenan induced rat paw oedema in Wistar rats at a dose level of 200mg / kg body weight administered orally. Significant anti-inflammatory activity was observed in aqueous extracts o...

The effect of gamma irradiation on pharmacological activity of non-irradiated and gamma-irradiated (at doses of 5 KGy and 10 KGy) samples of Ashwagandha were examined. Investigations were performed to study the anti-stress activity by forced swim endurance test in Albino Swiss mice at a dose level of 100mg / kg b.w. administered orally. Significant...

Liver, the largest organ in the body is being evolved to maintain the body's internal milieu and also protect itself from the challenges it faces during its functioning. It is a vital organ having diverse functions. It plays an important role not only in the metabolism, synthesis and storage but also in the detoxification of many endogenous and exo...

The antioxidant property of roots of Hemidesmus indicus (L.) R. Br. var pubescens (W. & A.) Hk. f., was evaluated by in vitro methods. Methanol (HIRM) and aqueous (HIRA) extracts of the drug were evaluated for in vitro antioxidant activity against DPPH, ABTS, hydrogen peroxide, nitric oxide and superoxide radicals. HPTLC fingerprinting studies were...

In the present study, the anti-inflammatory and analgesic effect of the methanol extract of Anogeissus acuminata leaf was investigated. The methanolic extracts of Anogeissus acuminata leaf were ingested orally (p.o.) in the form of suspension in 0.5% Tween 80 in two different doses, 200 and 400 mg/kg body weight). The anti-inflammatory effect of An...

To evaluate the ethanolic and aqueous extracts of Bauhinia variegata Linn. for in vitro antioxidant and antihyperlipidemic activity. Ethanolic and aqueous extracts of the stem bark and root of B. variegata Linn. were prepared and assessed for in vitro antioxidant activity by various methods namely total reducing power, scavenging of various free ra...

The purpose of the present work was to formulate and evaluate skin permeability of a glipizide gel to assess its suitability for transdermal delivery. A polymer gel was prepared by soaking hydroxypropyl methyl cellulose (5 g) overnight in phosphate buffer: ethanol (50:50, v/v) and mixing it with separately prepared glipizide solution in the same ve...

To evaluate the effect of sandalwood oil treatment during growth spurt period on learning and memory in Wistar rats. The effect of sandalwood oil on learning and memory was assessed using various methods viz., open field, passive avoidance, elevated plus maze and T-maze. The rats were administered sandalwood oil, with dose of 125mg/kg and 250mg/kg...

The study was designed to investigate analgesic and antipyretic activities of petroleum ether and alcohol extracts of Centratherum anthelminticum (L) Kuntze (family: Asteraceae) seeds (100 and 200 mg/kg, p.o.) in brewer's yeast-induced fever model in rats, acetic acid-induced writhing and Eddy's hot plate methods in mice. Both petroleum ether and a...

Ethanolic extract of Calotropis gigantea flowers (CGFE) was evaluated for hepatoprotective activity in mice. The CGFE (250 mg/kg bw and 500 mg/kg, bw p.o.) showed a remarkable hepatoprotective activity against paracetamol induced hepatotoxicity as judged from the serum markers for liver damage. Paracetamol induced a significant rise in aspartate am...

Liver, the largest organ in the body is being evolved to maintain the body's internal milieu and also protect itself from the challenges it faces during its functioning. It is a vital organ having diverse functions. It plays an important role not only in the metabolism, synthesis and storage but also in the detoxification of many endogenous and exo...

Silymarin, a flavonolignan from the seeds of 'milk thistle' (Silybum marianum), has been widely used from ancient times because of its excellent hepatoprotective action. It is a mixture of mainly three flavonolignans, viz, silybin, silidianin and silychristine, with silybin being the most active. Silymarin has been used medicinally to treat liver d...

The aim of the present study was to evaluate the analgesic activity of the ethanolic and aqueous extracts of the root of Bauhinia variegata Linn. The central and peripheral analgesic activity of the extracts was evaluated by Eddy's hot plate and acetic acid induced writhing models respectively. The reaction time and number of writhes produced were...

Hygrophila Spinosa (K. Schum) Heine (syn. Asteracantha longifolia Nees, Acanthaceae) was widely used in the Indian systems of medicine for the treatment of various liver ailments. The hepatoprotective activity of the aqueous extract of the stem was studied on carbon tetrachloride induced liver toxicity in rats. The activity was assessed by monitori...

Objective: To evaluate the chloroform extract of Calotropis gigantea flowers (CCGF) and ethanol extract of Calotropis gigantea flowers (ECGF) for their possible anti-inflammatory (AI) and anti-ulcer (AU) activities. Method: The anti-inflammatory effect of CCGF and ECGF (200mg/kg; p.o.) was investigated in carrageenan induced rat paw edema and cotto...

In the present study petroleum ether, chloroform and alcohol extracts of Centratherum anthelminticum (L.) Kuntze (family: Asteraceae) seeds (200 mg/kg b.w., p.o.) were tested for diuretic activity. The animals were grouped into five of six animals each. All the animals received priming dose of 0.9% sodium chloride solution (25 ml/kg b.w.). The firs...

Morinda citrifolia (Noni) has been traditionally used for bone and wound healing. The present study was carried out to investigate the effect of Morinda citrifolia leaves on experimental wounds and lipid peroxide levels in rats. Effect of aqueous extract of leaves of Morinda citrifolia (200 mg/kg) on excision, resutured incision and dead space woun...

Reservoir-type ocular inserts of brimonidine tartrate have been formulated by solvent casting technique for once a day administration. The drug-loaded core film was produced by casting a hydroalcoholic solution of hydroxyl propyl methyl cellulose (HPMC) (5% w/v) and glycerin (40% w/w). A 32 factorial design was employed to fabricate the rate-contro...

The newly synthesized thiazolidinone derivatives were evaluated for anti-ulcer and antibacterial activities. The anti-ulcer activity of thiazolidinone derivatives was screened by pylorus ligation and ethanol-induced ulcer methods in the doses of 20 and 30mg/kg. All the four test compounds showed significant percentage preventive ulcer ratio when co...

The present work is carried out to study the antioxidant potential of ethanolic extract of Alstonia scholaris Linn. (Apocynaceae) using various in vitro tests including 1, 1-diphenyl-2-picryl-hydrazil (DPPH.) free radical scavenging, metal ion chelating, hydrogen peroxide scavenging, superoxide anion radical scavenging, and ferric thiocyanate reduc...

Alstonia scholaris (Family: Apocynaceae) has been indicated for the treatment of various diseases, one among which is wound healing. The purpose of this study is to investigate the wound healing effect and antioxidant role of Alstonia scholaris leaves in animal models. The ethanol and aqueous extracts of Alstonia scholaris (EEAS, AEAS respectively)...

A 32 factorial design was employed to produce glipizide lipospheres by the emulsification phase separation technique using paraffin wax and stearic acid as retardants. The effect of critical formulation variables, namely levels of paraffin wax (X1) and proportion of stearic acid in the wax (X2) on geometric mean diameter (dg), percent encapsulation...

Objective: The purpose of the present study was to evaluate food intake inhibitory activity of aqueous extract of Sida cordifolia (AESC) and alcoholic extract of Withania somnifera (AEWS). Both Sida cordifolia Linn. (Malvaceae) and Withania somnifera Linn. (Solanaceae) are widely growing medicinal plants and have been reported to possess number of...

A 32 factorial design was employed to produce glipizide lipospheres by the emulsification phase separation technique using paraffin wax and stearic acid as retardants. The effect of critical formulation variables, namely levels of paraffin wax (X1) and proportion of stearic acid in the wax (X2) on geometric mean diameter (dg), percent encapsulation...

The chloroform extract of dried leaves of Trichilia connaroides , was screened for analgesic and antiinflammatory activity, using chemical-, thermal- and formalin-induced inflammation in Swiss mice and Wistar rats. Chloroform extract showed significant and dose- dependent analgesic, and antiinflammatory activity.

The aim of this study was to evaluate the antiarthritic activity of ethanolic extract of the leaves of Crotalaria juncea (CJE) in Complete Freund's Adjuvant (CFA) induced arthritis model in rats, and also to evaluate anti-ulcerogenic activity of CJE.Arthritis was induced in male albino Wistar rats by injection of CFA (0.1 mL) into the left foot pad...

An affinity chromatographic method with a novel eluant from Bacillus licheniformis is described. α-amylase was bound to starch, starch-celite, starch-Sepharose columns and the bound α-amylase was rapidly eluted with 2% (w/v) white dextrin. The binding capacity of α-amylase to starch column is 380 μmol/g of starch. The purified enzyme showed a singl...

Hypocholesterolemic activity was studied on normal rats for hexane, chloroform and methanol extracts of Trichilia connaroides (W. & A.) Bentilezen. The parameters studied include free cholesterol, triglycerides and high density lipoprotein levels in blood. Only hexane and methanol extracts produced a rise in high density lipoprotein level (p<0.05 a...

A combination of chromatofocusing and gel filtration chromatography resulted in a simple purification of -amylase from Bacillus licheniformis. The purification was approximately 77-fold. Identification of the purity was established by SDS–PAGE. Molecular weight and isoelectric point of the purified enzyme were 58 kDa and 7.18 respectively. Western...