Pumtiwitt Mccarthy

Pumtiwitt Mccarthy
Morgan State University · Department of Chemistry

20.21
 · 
PhD

About

28
Publications
1,779
Reads
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284
Citations
Introduction
Pumtiwitt Mccarthy currently works at the Department of Chemistry, Morgan State University. Pumtiwitt does research in biochemistry to optimize enzymes for vaccine development and bioremediation.
Research Experience
August 2013 - present
Morgan State University
Position
  • Professor (Assistant)
Description
  • Research in the McCarthy lab focuses on carbohydrates in the context of infectious disease. We seek to use enzymes from Neisseria meningitidis as potential synthetic tools for carbohydrate-based therapeutics and biomaterials.
January 2009 - June 2013
U.S. Food and Drug Administration
Position
  • NIH PRAT Postdoctoral Research Fellow/ORISE Postdoc.
August 2003 - February 2009
University of Delaware
Position
  • Graduate Research Assistant
Education
August 2003 - February 2009
University of Delaware
Field of study
  • Biochemistry
August 2001 - May 2003
Rowan University
Field of study
  • Biochemistry

Publications

Publications (28)
Article
Full-text available
Neisseria meningitidis causes most cases of bacterial meningitis. Meningococcal meningitis is a public health burden to both developed and developing countries throughout the world. There are a number of vaccines (polysaccharide-based, glycoconjugate, protein-based and combined conjugate vaccines) that are approved to target five of the six disease...
Article
Neisseria meningitidis is a Gram-negative bacterium that causes meningitis. Our overall goal is increased understanding of the N. meningitidis serogroup W capsule polymerase. This enzyme creates the capsular polysaccharide found in this serogroup. During the reaction, the enzyme transfers galactose and sialic acid from two nucleotide donor sugars (...
Article
Full-text available
Objective: Meningococcal meningitis is a public health burden. Immunization strategies have reduced global incidence of the disease. Glycoconjugate vaccines are the most effective type of vaccine to combat most causes of meningococcal meningitis. These vaccines contain capsular polysaccharide fragments from disease-causing serogroups of Neisseria...
Article
Full-text available
Heavy metal pollution of water is a significant environmental and public health concern. Current biological strategies for heavy metal removal from water are performed using microbial biopolymers, including polysaccharides, that are already fully formed. This creates limitations in adapting polysaccharides to increase binding affinity for specific...
Article
Full-text available
Cisplatin and other metal-based drugs often display side effects and tumor resistance after prolonged use. Because rhenium-based anticancer complexes are often less toxic, a novel series of organorhenium complexes were synthesized of the types: XRe(CO)3Z (X = α-diimines and Z = p-toluenesulfonate, 1-naphthalenesulfonate, 2-naphthalenesulfonate, pic...
Article
Full-text available
Neisseria meningitidis serogroups A, B, C, Y, W135 and X are responsible for most cases of meningococcal meningitis. Neisseria meningitidis serogroup X has recently emerged as a contributor to outbreaks of disease in Africa, but there is currently no vaccine against serogroup X. Understanding of the biosynthesis of the serogroup X capsular polysacc...
Article
Vaccination with meningococcal glycoconjugate vaccines has decreased the incidence of invasive meningitis worldwide. These vaccines contain purified capsular polysaccharides attached to a carrier protein. Because of derivatization chemistries used in the process, conjugation of polysaccharide to protein often results in heterogeneous mixtures. Well...
Article
Full-text available
Vaccines against Neisseria meningitidis group C are based on its α-2,9-linked polysialic acid capsular polysaccharide. This polysialic acid expressed on the surface of N. meningitidis and in the absence of specific antibody serves to evade host defense mechanisms. The polysialyltransferase (PST) that forms the group C polysialic acid (NmC PST) is l...
Article
Abstract Bacterial polysaccharides are components of glycoconjugate vaccines against encapsulated bacterial pathogens. Because these vaccines are prepared by random coupling methods the structure of the immunogens is difficult to characterize. As a model for the development of well-defined glycoconjugates we have devised a chemoenzymatic method for...
Article
The success of arsenic trioxide in the treatment of acute promyelocytic leukemia has renewed interest in the cellular targets of As(III) species. The effects of arsenicals are usually attributed to their ability to bind vicinal thiols or thiol selenols in prefolded proteins thereby compromising cellular function. The present studies suggest an addi...
Article
The flavin-dependent quiescin-sulfhydryl oxidase (QSOX) inserts disulfide bridges into unfolded reduced proteins with the reduction of molecular oxygen to form hydrogen peroxide. This work investigates how QSOX and protein disulfide isomerase (PDI) cooperate in vitro to generate native pairings in two unfolded reduced proteins: ribonuclease A (RNas...
Article
The Quiescin-sulfhydryl oxidase (QSOX) family of flavoenzymes catalyzes the direct and facile insertion of disulfide bonds into unfolded reduced proteins with concomitant reduction of oxygen to hydrogen peroxide. This review discusses the chemical mechanism of these enzymes and the involvement of thioredoxin and flavin-binding domains in catalysis....
Article
Adenylosuccinate lyase (ASL) of Bacillus subtilis contains three conserved histidines, His(68), His(89), and His(141), identified by affinity labeling and site-directed mutagenesis as critical to the intersubunit catalytic site. The pH-V(max) profile for wild-type ASL is bell-shaped (pK (1) = 6.74 and pK (2) = 8.28). Only the alkaline side changes...

Projects

Projects (2)
Project
Enzymatic synthesis of modified bacterial polysaccharides for use as eco-friendly bioremediation agents in heavy-metal polluted water.
Project
Biochemical characterization and optimization of N. meningitidis capsule-producing enzymes to produce well-defined glycoconjugate vaccines.