Pulok K Mukherjee

Pulok K Mukherjee
Institute of Bioresources and Sustainable Development | IBSD · Department of Biotechnology

M. Pharm, PhD, FRSC

About

350
Publications
186,526
Reads
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15,428
Citations
Citations since 2017
71 Research Items
7405 Citations
201720182019202020212022202302004006008001,0001,2001,400
201720182019202020212022202302004006008001,0001,2001,400
201720182019202020212022202302004006008001,0001,2001,400
201720182019202020212022202302004006008001,0001,2001,400
Introduction
Professor Pulok K. Mukherjee is working on traditional medicine inspired drug discovery from Indian medicinal plants to make them available from ‘Farm to Pharma’. His research on validation of medicinal plants from India systems of medicine, their formulation and standardization, which are useful bio-prospecting tools for the traditional medicine based drug discovery programme. He has to his credit > 200 publications with over 18600 citations. For more please visit www.pulokmukherjee.in
Additional affiliations
February 2020 - present
Institute of Bioresources and Sustainable Development
Position
  • Managing Director
June 2006 - February 2020
Jadavpur University
Position
  • Managing Director
December 2001 - February 2020
Jadavpur University, Kolkata, India
Position
  • Professor

Publications

Publications (350)
Article
Full-text available
Mollugo oppositifolia Linn. is traditionally used in neurological complications. The study aimed to investigate in-vitro neuroprotective effect of the plant extracts through testing against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and β-secretase linked to Alzheimer’s disease (AD). To understand the safety aspects, the extracts we...
Article
Introduction: Ginger constitutes the rhizome part of the plant Zingiber officinale from the Zingiberaceae family. A large number of ginger varieties with high sensorial and functional quality are found in Northeast India. Hence, phytopharmacological screening of different ginger varieties is essential that will serve as a guideline in applied rese...
Article
Full-text available
This study aimed to profile the metabolites, piperine content, and biological activities (antioxidant, anti-inflammatory, anticancer, antibacterial, and apoptotic cell) of 15 samples of Piper nigrum L. from various geographic regions of Thailand. UHPLC-QTOF-MS was applied to establish alkaloid profiles of the P. nigrum L. samples. PCA and HCA analy...
Article
The different varieties of melons (Cucumis melo L.) have been used in various traditional systems of medicine for decades to treat different ailments, including inflammation, cancer, cardiovascular, diabetes, edema, etc. The present study was designed for the quantification of cucurbitacin E in five different varieties of melon fruit through a vali...
Article
Quality assessment of medicinal, aromatic plants and plant parts, and spices are to large extent based on estimation of active molecules. Such estimations require high cost, time consuming and laboratory bound methods like High Performance Liquid Chromatography. These methods can handle very few samples at a time and have no portability. Raman spec...
Article
Phycocyanin is a water-soluble blue pigment biliprotein that has been utilized as a nutritional supplement for its potent antioxidant and anti-inflammation activity. In this study, three cyanobacterial species from the North-Eastern India, Calothrix sp. (BTA-476), Calothrix sp. (BTA-857), and Westiellopsis sp. (BTA-801) are taxonomically and molecu...
Article
Ethnopharmacological relevance Lagenaria siceraria Stand. (Family: Cucurbitaceae), popularly known as bottle gourd, is traditionally used in Ayurvedic medicine as a food plant, especially in hypertension and obesity. Aim of the study Investigations were undertaken to assign novel lead combinations from this common food plant to multi-molecular mod...
Article
Full-text available
Background: This study comparatively assessed seven indigenous traditional tea plants on several attributes that included antioxidant, nutritional, caffeine contents, and cyclooxygenase activity. Methodology: Nutritional content of all tea plants were determined for energy, fat, carbohydrates, total sugars, dietary fiber and amino acids. Antioxidan...
Article
Parkia roxburghii pod is a very popular food in North East India, known as Yongchak. Traditionally, it is used for the treatment of blood pressure, piles, diabetes, dysentery and urinary tract infections. The seeds contain many phytochemicals like flavonoids, alkaloids, saponins, terpenoids and tannins which are responsible for their medicinal prop...
Article
Full-text available
A South African traditional formulation, PHELA®, is consumed by the traditional people for severe chest problems with coughing, diarrhea, oral ulcers etc. The present study focused on establishing the anti-infective properties of a safe and standardized poly-herbal formulation through a series of criteria and specifications.
Article
Piperine is the most important alkaloid molecule present in Piper nigrum. In this study, a simple, rapid, quantitative high-performance thin-layer chromatography (HPTLC) technique proposed by International Organization for Standardization (ISO) was optimized and validated for the quantitative estimation of piperine in P. nigrum. Densitometric analy...
Article
A rapid validated UFLC-PDA method was developed to identify and quantify ayapanin (AY) and umbelliferone (UM) simultaneously in Ayapana triplinervis (Vahl) methanolic extract (ATME). The method was validated for linearity, limit of detection (LOD=3:1σ/S), limit of quantification (LOQ=10:1σ/S), precision, accuracy, specificity and robustness. The re...
Article
Background : The worldwide corona virus disease outbreak, generally known as COVID-19 pandemic outbreak resulted in a major health crisis globally. The morbidity and transmission modality of COVID-19 appear more severe and uncontrollable. The respiratory failure and following cardiovascular complications are the main pathophysiology of this deadly...
Chapter
Plants are rich source of various therapeutic agents. These are potent compounds having a wide range of pharmacological properties. However, their clinical use is limited owing to the poor oral bioavailability and pharmacokinetic properties of these phytoactives. To overcome this, phospholipid complexation of phytoactive constituents have been util...
Chapter
Traditional analytical methods are not enough to handle the increasing number of problems in natural product research. The hyphenated technique is an advanced form which is the link of a separation and spectroscopic technique to achieve the benefits of both. The outstanding progresses in hyphenated systems have considerably extended their uses in n...
Chapter
Ayurveda is the science that revolves around the sustainability of life since the manifestation of the universe. This knowledge of life was first apprehended with documentary evidence in Atharva Veda which specifically deals with treatments against spells/diseases. This is the last Veda considered among the Chatur Vedas. Similarly, the diagnostic t...
Chapter
Herbal medicines are botanical mixtures which present synergies of poly-pharmacological phyto-combinations are more prevalent in developing countries. They modulate the activity of target networks of underlying disease phenotypes are efficient than purified compounds due to favorable “synergistic” interactions. This chapter emphasis on methodical,...
Chapter
Golden era of antibiotics started following the accidental discovery of penicillin by Alexander Fleming. Enormous numbers of antibiotics have been discovered to tackle various types of infections. However, microorganisms are continuing to develop resistance to the existing antibiotics. Because of this antibiotic resistant issue, infectious disease...
Chapter
Central cholinergic pathway is involved in learning and memory. The creation of severe cholinergic deficits due to neurodegenerative disorders resulting in cognitive impairment contribute to symptomatic cholinergic therapy. Inhibition of brain cholinesterase serves as a strategy for treating several cognitive diseases such as Alzheimer's disease (A...
Chapter
Skin aging is the result of programmed senescence and prolonged environmental injury to skin. Human skin is continuously exposed to environmental influences and is therefore subjected to both intrinsic as well as extrinsic aging processes. Aged skin is characterized by loss of skin tone and resilience, increased roughness and dryness, irregular pig...
Chapter
Medicinal plants are used for the preparation of herbal medicines for a long time. But due to the therapeutic efficacy and other distinct advantages over conventional medicines, these herbal medicines have gained much more attention nowadays. Development of good quality herbal drugs requires thorough investigation of medicinal plants from the persp...
Chapter
Because of their unique effects and relatively low side effects, herbal medicine has been gaining popularity all over the world. Quality control is a challenge to ensure safety, efficacy and batch-to-batch consistency of herbal products due to the complexity of phyto-chemical constituents. Generally, it is believed that the risk associated with her...
Chapter
The use of traditional medicines and traditional knowledge in Africa is constantly on the rise, now with new greater interest from the international herbal markets for herbal medicinal and nutraceutical products from Africa. Although there is some growing scientific studies conducted on African traditional medicines, these studies are few and mainl...
Article
Full-text available
Background Ursolic acid (UA) is a potent plant-based hepatoprotective agent having poor bioavailability, which hampers its therapeutic efficacy. The present study tries to overcome this limitation by combining it with piperine (PIP), a proven bioenhancer and hepatoprotective agent. Methods The type of interaction (synergism, addition, or antagonis...
Article
Ethnopharmacological relevance Medicinal plants of Cucurbitaceae family consist of several edible fruits and vegetables consumed worldwide since ancient times. The plants of this family have played an essential role in the ethnopharmacological as well as traditional medicinal system globally and their evidence is well established in several traditi...
Article
ntroduction The major chemical marker of black pepper (Piper nigrum L) is piperine and its estimation is extremely important for quality assessment of black pepper. The methods for piperine quantification, to date, are laboratory based and use high end instruments like chromatographs, which require tedious sample processing and cause sample destru...
Article
Full-text available
Translation of traditional medicine is moving away from the reductionist approach towards harnessing the synergy of poly-pharmacological phyto-combinations that modulate the activity of target networks of underlying disease phenotypes. Metabolomics enabled the profiling of herbal extracts and formulations in high resolution through LC-MS/MS, GC-MS...
Article
The Eastern Himalayan regions of India, Nepal and Bhutan have more than 200 varieties of unsurpassed ethnic fermented foods and alcoholic beverages, which are lesser known outside the world. However, these ethnic foods are region- and community-specific, unique and some are exotic and rare, which include fermented vegetables, bamboo shoots, soybean...
Article
Photo-oxidative skin damage is mainly caused by the UV-A radiation of the sun. Synthetic sunscreens used to counter this acts mostly on the superficial skin layer and possess serious side effects. P-coumaric acid (PCA) is a UV-A protective plant phenolic having quick diffusion and distribution in superficial skin layers limiting its application as...
Article
Full-text available
Andrographis paniculata (Burm. F) Nees, has been widely used for upper respiratory tract and several other diseases and general immunity for a historically long time in countries like India, China, Thailand, Japan, and Malaysia. The vegetative productivity and quality with respect to pharmaceutical properties of Andrographis paniculata varies consi...
Article
Cucumis sativus L. of the Cucurbitaceae family, commonly known as cucumber, is commercially cultivated worldwide. The major phytoconstituents present in the Cucurbitaceae family are different curcurbitacins, principally cucurbitacin E. The content of cucurbitacin E differs within the species or cultivars due to factors like genetic variation and ge...
Chapter
Traditional medicine has a long history. The World Health Organization (WHO) recognizes that traditional medicine (TM) is a vital part of the global health care system. As per WHO recommendation, TM should be a part of health and wellness and promote safe and effective use. It can be done by proper regulation, research and integrate products, pract...
Article
Ethnopharmacological relevance Trimada is well-known polyherbal Ayurvedic formulation used in Indian Traditional medicine since ancient times. It consisted of three inebriant herbs including “Chitraka” (Plumbago zeylanica Linn. Family- Plumabaginaceae), “Musta” (Cyperus rotundus Linn. Family- Cyperaceae) and Vidanga (Embelia ribes Burm. F. Family-...
Article
Full-text available
Introduction Immunity boosting has emerged as a global strategy to fight the SARS‐CoV‐2 pandemic situation. In India, AYUSH systems of medicine have been promoted as an immune‐protection strategy. Andrographis paniculata (Burm. F) Nees (AP) mentioned in Ayurveda has been widely used for treating sore throat, flu, and upper respiratory tract infecti...
Article
Piper species is one of the most widely consumed spices for culinary purposes. Piperine (PIP) present in Piper species has a wide range of therapeutic activity including hepatoprotection. However, the major biological limitation of PIP is its low bioavailability after oral administration. Purpose of the study was to prepare an optimized and adequat...
Article
Medicinal plants of the Cucurbitaceae family are widely consumed as foods in human diet. In the present study, a simple, rapid, quantitative, and validated high-performance thin-layer chromatographic (HPTLC) method has been developed for the estimation of chlorogenic acid (CA) in the hydroalcoholic fruit extracts of Luffa acutangula (sponge gourd),...
Chapter
Diabetes mellitus has emerged as a major health challenge all over the world. The disease condition is a byproduct of fast, stressful lifestyle away from nature. In traditional system of medicine like the Ayurveda, the history of the disease “Madhumeha” is recorded. In the Ayurveda several single herbs (51), bhasma metals and minerals (9), herbo-mi...
Article
Full-text available
Introduction Lagenaria siceraria , is a popular food plant among Indians, contains a large number of phenolic compounds with several medicinal benefits, mentioned in Indian System of Medicine (ISM). Objectives To investigate the carbonic anhydrase inhibitory potential and inhibitory mechanism of the most potent fraction of L. siceraria fruits. Ma...
Article
PHELA is a South African traditional medicine formulation. The decoction and infusion with water is consumed orally by persons with compromised immune systems. The present investigation is based on different approaches of exploitation and exploration of PHELA for cholinesterase inhibition activity based on its biomarker profiling. The extract was s...
Chapter
Full-text available
The history of medicine is ancient, the sum total of the knowledge, skills, and practices based on the theories, beliefs, and experiences indigenous to different cultures, used in the maintenance of health, as well as in the prevention, diagnosis, improvement, or treatment of physical and mental illnesses. The terms complementary/alternative/noncon...
Chapter
Quality assessment and scientific validation of medicinal plants are essential parameters to be considered in ensuring the quality, efficacy, and safety of herbal products at large. In search of newer therapeutic leads, enzymes are considered to be an attractive target for drug development due to their essential catalytic roles in many physiologica...
Book
Full-text available
Quality Control and Evaluation of Herbal Drugs brings together current thinking and practice for evaluation of natural products and traditional medicines. The use of herbal medicine in therapeutics is on the rise in both developed and developing countries and this book facilitates the necessary development of quality standards for these medicines.T...
Article
Background: In Ayurvedic and Unani texts, many herbs and formulations are mentioned as complexion promoters having skin brightening and whitening effects that downregulate melanin synthesis. However, with the assistance of chromatographic techniques, further validation of scientific standardization is required. Objective: To validate individual...
Article
Full-text available
Background: The seed of Trigonella foenum-graecum L. (Methika in Sanskrit) is a well known kaphahara (balancing kapha) herb in Ayurveda indicated in Prameha or early diabetes mellitus. It is also useful in obesity and reduces lipid level of blood. Purpose: We aimed to explore the metabolites present in the plant extract and to establish the combina...
Article
A simple, rapid, quantitative, and validated high-performance thin-layer chromatographic (HPTLC) method has been developed for the simultaneous estimation of quercetin (QU), rutin (RU), and gallic acid (GA) in the methanolic extract of Amaranthus tricolor L. aerial parts. Densitometric analysis of QU, RU, and GA were carried out in the absorbance m...
Article
Full-text available
Background: The consumption of the fruits of cucurbitaceae plants is widely popular among Indians due to their various nutritional and medicinal purposes. Some of these plants are well reported in Ayurveda due to their potential therapeutic importance. In particular, the plants of this family are well-characterized by the presence of its bitter pr...
Article
Objective: To prepare and characterize an optimized phospholipid complex of Ursolic acid (UA) to overcome the poor pharmacokinetic properties and to investigate the impact of the complex on hepatoprotective activity and bioavailability in animal model. Significance: UA is a potential phytoconstituent obtained from several plant sources, which has b...
Article
Full-text available
Background Viscum articulatum Burm. (Family: Loranthaceae) is commonly known as mistletoe. In ayurveda, the plant parts are used in “Kapha”, “Vata”, diseases of the blood, ulcer, and epilepsy. The plant parts are also used in urinary tract infection and wound infection. The plant contains five triterpenoids such as α-amyrin, lupeol, betulin, betuli...
Article
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The different parts of Momordica charantia are well reported for its several therapeutic applications against hyperglycemia, hypercholesterolemia associated with pancreatic lipase (PL). Inhibition of this enzyme prevents the absorption of dietary triglyceride in the intestine, and thus exerts the anti‐obesity effect. This study was aimed to investi...
Article
Introduction Luffa acutangula (L.) Roxb, commonly known as ridge gourd (cucurbitaceae), is a common vegetable cultivated in India. It is also a well‐used medicinal plant in Indian traditional medicine. Objectives To analyse the phenolics content of the most potent carbonic anhydrase‐inhibiting fraction from an extract of L. acutangula. Materials...
Article
Background Herpes Simplex Virus (HSV), a highly contagious pathogen, is responsible for causing lifelong oral to genital infection in human. Boswellia serrata oleo-gum-resin possesses a strong traditional background of treating diverse skin ailments including infection but its effect on HSV-1 has not been examined yet. Purpose To exploit its poten...
Article
Tyrosinase is a key regulatory multifunctional enzyme containing copper that is responsible for the biosynthesis of melanin that determines the color of the skin. Accumulation of excessive melanin causes various dermatological disorders including melasma and age spots. Also, tyrosinase is responsible for enzymatic browning reactions in damaged frui...
Article
Full-text available
Background & objectives: The existing antileishmanial drugs for complete cure of visceral leishmaniasis (kala-azar) are limited. The available drugs are either toxic or less effective leading to disease relapse or conversion to post-kala-azar dermal leishmaniasis. Several herbal extracts have been shown to have antileishmanial activity, but a herb...
Article
Full-text available
The development of traditional medicine with the perspectives of safety, efficacy and quality would help not only to preserve the traditional heritage but also to rationalize the use of herbal medicine in the human healthcare. Nature is considered as a compendium for templates of new chemical entities. The medicinal plants mentioned in the differen...
Chapter
Evidence-based validation of the ethno-pharmacological claims on traditional medicine (TM) is the need of the day for its globalisation and reinforcement. Combining the unique features of identifying biomarkers that are highly conserved across species, this can offer an innovative approach to biomarker-driven drug discovery and development. TMs are...
Article
Betulinic acid (BA) is a potent bioactive compound and well known for its therapeutic activity. However, its efficacy is restricted due to low solubility as well as poor bioavailability. The aim of the study was to develop a BA loaded nanoemulsions for enhanced bioavailability and hepatoprotective activity. The optimized nanoemulsion (BA-NE1) was c...
Article
The fruit of Dillenia indica L. is extensively used as a food additive. Betulinic acid (BA) is the most prominent secondary metabolite present in D. indica. This study screened the bioassay guided isolation of BA from D. indica and explored its tyrosinase inhibitory mechanism. Half maximal inhibitory concentration (IC50) of BA were calculated as 13...
Article
Full-text available
Capsaicin is a pungent capsacinoid which differs in capsicum fruit within the species and the cultivars due to its topographical diversity. Here, a comparative estimation of capsaicin in seven varieties of capsicum has been performed by RP-HPLC method. The method was carried out in reverse phase C18 column using acetonitrile and water (1% acetic ac...
Chapter
The development of functional food (medicinal plants, marine sources, vitamins, minerals, fish oils, plant-based oils, amino acids, etc.) as nutraceutical and dietary supplements are gaining momentum with the perspectives of safety, efficacy, and quality for promotion of health of human beings. Nutraceuticals are diet supplements that deliver an en...
Article
Herbal medicines have been used from ancient times for the management of several disease and ailments along with prescribed medicine without consulting the physicians. Berberis aristata DC. has been widely used in management of diabetes as alternative therapy in India and other Asian countries. An approach has been made to evaluate the possible cyt...
Article
Full-text available
Secondary metabolites from plants provide lead molecules for drug development. Metabolomics is a modern omic-technique for comprehensive analysis of phytochemicals. Advances in mass spectrometry (MS) based platforms like GC-MS and LC-MS, helped in separation and identification of several metabolites. Such analysis can be a valuable tool for identif...
Article
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Introduction: Stereospermum suaveolens Roxb., Viscum articulatum Burm., Annona squamosa, Capsicum annuum cayenne, Ananas comosus Merrill. are used for the management of microbial infection in Ayurveda. The present study was designed to standardize the extract of S. suaveolens bark (SSB), V. articulatum aerial part (VAAP), A. squamosa leaf (ASL), C....
Article
Ethnopharmacological relevance: Ayurveda entails a scientific tradition of harmonious living and its origin can be traced from ancient knowledge in Rigveda and Atharvaveda. Ayurveda is a traditional healthcare system of Indian medicine since ancient times. Several Ayurvedic medicines have been exploiting for treatment and management of various dis...
Article
Full-text available
Objective Tinospora cordifolia is used for treatment of several diseases in Indian system of medicine. In the present study, the inhibition potential of T. cordifolia extracts and its constituent tinosporaside to cause herb-drug interactions through rat and human liver cytochrome enzymes was evaluated. Materials and Methods Bioactive compound was...
Article
Ethnopharmacological relevance: Ayurveda (Sanskrit: Ayus - life + Veda - knowledge) means the "True knowledge of life". Ayurveda deals with a complete self-sustainable system of medicine. The Government of India through its Ministry of AYUSH is responsible for policy formulation, development and implementation of programs for the growth, developme...