Priyankar Sen- Doctor of Philosophy
- Professor (Associate) at Vellore Institute of Technology University
Priyankar Sen
- Doctor of Philosophy
- Professor (Associate) at Vellore Institute of Technology University
Amyloid detection kit
About
54
Publications
51,972
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Introduction
To translate the knowledge of Protein Biochemistry to solve real world problems
Current institution
Additional affiliations
September 2012 - present
Vellore Institute of Technology
Position
- Professor (Associate)
March 2009 - October 2010
September 2004 - August 2009
Education
December 2004 - August 2009
July 2002 - July 2004
P.G. Department of Biotechnology, Utkal University
Field of study
- Biotechnology
Publications
Publications (54)
The long-term impact of the COVID-19 pandemic concerns risk to human health, particularly its potential association with protein misfolding and amyloidosis. This review article explores the causality relationship between SARS-CoV-2 infection, and protein misfolding, leading to amyloid-related conditions. It delves into the mechanisms by which viral...
![Kinematics of Amyloid Formation [3, 4]. In the lag Phase, Monomers such...](publication/381109608/figure/fig1/AS:11431281273575282@1724637071552/Kinematics-of-Amyloid-Formation-3-4-In-the-lag-Phase-Monomers-such-as-A-Native_Q320.jpg)
![Production Aβ from APP and neurodegeneration [13]. (a) APP, a...](publication/381109608/figure/fig2/AS:11431281273511461@1724637071911/Production-Ab-from-APP-and-neurodegeneration-13-a-APP-a-single-pass-transmembrane_Q320.jpg)
Modern medicine has increased the human lifespan. However, with an increase in average lifespan risk of amyloidosis increases. Amyloidosis is a condition characterized by protein misfolding and aggregation. Early detection of amyloidosis is crucial, yet conventional diagnostic methods are costly and lack precision, necessitating innovative tools. T...
Amyloid fibrils are formed from various pathological proteins. Monitoring their aggregation process is necessary for early detection and treatment. Among the available detection techniques, fluorescence is simple, intuitive, and convenient due to its sensitive and selective mode of detection. It has certain disadvantages like poor photothermal stab...
Polyphosphate polymers are chains of phosphate monomers chemically bonded together via phosphoanhydride bonds. They are found in all prokaryotic and eukaryotic organisms and are among the earliest, most anionic, and most mysterious molecules known. They are everywhere, from small cellular components to additives in our food. There is a strong assoc...
Caffeic acid (CA) is a phenolic acid found in a variety of foods. In this study, the interaction mechanism between α-lactalbumin (ALA) and CA was explored with the use of spectroscopic and computational techniques. The Stern-Volmer quenching constant data suggest a static mode of quenching between CA and ALA, depicting a gradual decrease in quenchi...
Picloram (PC) is a systemic herbicide that controls herbaceous weeds and woody plants. HSA, the most abundant protein in human physiology, binds to all exogenic and endogenic ligands. PC is a stable molecule (t1/2~157-513 days) and a potential threat to human health via the food chain. HSA and PC binding study has been done to decipher the location...
The outburst of Novel Coronavirus disease 2019 (COVID-19) due to SARS-CoV-2 has caused havoc throughout the world. There has been an increase in the number of COVID-19 cases due to the adaptation of the virus in any environment. Currently, supportive care and non-specific treatment to ameliorate the symptoms of the patient are being considered. The...
The emergence of pandemics like SARS-CoV-2 and a gradual increase in Multidrug Resistant (MDR) infections highlights the need of innovation in therapeutics. Antibodies are one of the potential solutions for long. Antibody therapy has come very long way from the fight against infectious diseases, bacterial toxins to hybridoma technology and monoclon...
Background
Anti-TNF-α scFv is gaining acceptance as an effective drug for various diseases such as rheumatoid arthritis and Crohn’s disease that involve elevated levels of TNF-α. The single-chain variable fragment (scFv) consists of variable regions of heavy and light chains of monoclonal antibodies (mAb). Due to its smaller size, it curbs the mAb’...
The Lichen, Parmelia sulcata synthesizes various secondary metabolites, in which phenolic based compounds received much attention due to their importance in biomedical application. Especially the phenolic compound was effective against the cancer treatment. An effective administration of such plant natural product can represent a significant conven...
Bovine liver catalase (BLC), a homotetramer (Mr = 28,000), is an industrially and pharmaceutically important enzyme. A cationic Gemini surfactant (tetramethylene-1,4-bis(dimethyltetradecylammonium bromide); 14-4-14) was found to induce aggregation in BLC, as confirmed by Rayleigh light scattering and dynamic light scattering up to ∼150 μM, at a cri...
Tartrazine (Tz) is a yellow food color dye that is used in a range of foods to make them more appealing and attractive. However, very limited information is there about its interaction with macromolecules, like different proteins that are components of the food products. In this work the bovine beta-lactoglobulin (BLG) aggregation stimulating prope...
Amyloid fibril formation by proteins and their deposition in cells and tissues are associated with several amyloid-based disorders. Understanding the mechanism of amyloid fibril formation is thus of the utmost importance for the designing ligands that could prevent or inhibit the fibrillation process and help to treat of such disorders. We describe...
Although many reports have described protein–surfactant interactions but the mechanism of amyloid-like fibril formation by surfactants is not clear. In this study, we investigated the role of sodium dodecylbenzene sulfonate (SDBS) to induce aggregation in equine heart muscle myoglobin (E–Mb) at pH 4.5. The aggregation was analyzed by Rayleigh scatt...
The aim of this study was to investigate the effects of sodium dodecyl benzenesulfonate (SDBS) on hen egg white lysozyme (HEWL) fibrillogenesis at pH 7.4. HEWL fibrillogenesis in the presence of SDBS was characterized using several spectroscopic techniques (turbidity, light scattering, intrinsic fluorescence, ThT binding assay, ThT kinetics, far-UV...
Allura red (AR) is an artificial azo dye mostly used in food industries and has potential health risks.We examined the role of AR in amyloidogenesis using hen eggwhite lysozyme (HEWL) at pH 7.0. The amyloidogenic induction properties of AR in HEWL were identified by circular dichroism (CD), turbidity, intrinsic fluorescence, light scattering, trans...
Streptozocin (STZ) is a broad range antibiotic, highly genotoxic, antineoplastic and hyperglycemic. HSA is the most abundant protein in physiology and it binds to almost all exogenic and endogenic ligands, including drugs. STZ-induced fluorescence quenching of HSA has been done at pH 7.4, pH 3.5 and at pH 7.4 with 4.5M urea at temperatures 286K, 29...
β-lactoglobulin (BLG) is a well characterized milk protein and a model for folding and aggregation studies. Rutin is a quercetin based-flavanoid and a famous dietary supplement. It is a potential protector from coronary heart disease, cancers, and inflammatory bowel disease. In this study, amyloid fibrillation is reported in BLG at pH 2.0 and tempe...
Streptozocin (STZ) is a broad range antibiotic, highly genotoxic, antineoplastic and hyperglycemic. HSA is the
most abundant protein in physiology and it binds to almost all exogenic and endogenic ligands, including
drugs. STZ-induced fluorescence quenching of HSA has been done at pH 7.4, pH 3.5 and at pH 7.4 with 4.5 M
urea at temperatures 286 K,...
Anti-TNFα ScFv has been proven to be a panacea in various disorders which involve elevated levels of TNFα such as rheumatoid arthritis (RA) and crohn’s disease (CD). Single chain variable fragment (ScFv) constructs are small molecules derived from monoclonal antibodies which curb the limitation of penetration into the tissues during neutralization...
Surfactant-induced amyloid fibrillation has many implications in laboratory and industry. Previously, cetyltrimethylammonium bromide (CTAB; C19H42BrN) induced amyloid formation in concanavalin A (ConA) has been reported by authors (Khan, JM et al., 2016 A). In this work, cationic gemini surfactants pentanediyl‑1,5‑bis (dimethylcetylammonium bromide...
Sodium dodecyl sulfate (SDS) is an anionic surfactant that can be used to stimulate protein fibrillation in vitro. Here, we investigated the effects of SDS on camel IgG aggregation at pH 3.5 and 7.4. SDS-induced amyloid fibril formation in camel IgG was examined by turbidity measurements, Rayleigh scattering, Thioflavin T (ThT) fluorescence, intrin...
Protease inhibitors are one of the most promising and investigated subjects for their role in pharmacognostic and pharmacological studies. This study aimed to investigate antioxidant, anti-inflammatory, and antimicrobial activities of trypsin inhibitors (TIs) from two plant sources (Cajanus cajan and Phaseolus limensis). TI was purified from C. caj...
Amyloid fibrils are playing key role in the pathogenesis of various neurodegenerative diseases. Generally anionic molecules are known to induce amyloid fibril in several proteins. In this work, we have studied the effect of anionic food additive dye i.e., tartrazine (TZ) on the amyloid fibril formation of human serum albumins (HSA) and bovine serum...
The bindings of detemir [LysB29(Nε-tetradecanoyl)des(B30)-insulin] with two highly
homologous albumins, HSA (human serum albumin) and BSA (bovine serum albumin), were
investigated through CD, spectrofluorophotometry, and molecular docking analysis. The absence
of any tryptophanyl residue in detemir makes albumin binding study possible by exclusive...
Amyloid fibril formation is responsible for several neurodegenerative diseases and are formed when native proteins misfold and stick together with different interactive forces. In the present study, we have determined the mode of interaction of the anionic surfactant sarkosyl with hen egg white lysozyme (HEWL) [EC no. 3.2.1.17] at two pHs (9.0 and...
The bindings of detemir [LysB29(Nε-tetradecanoyl)des(B30)-insulin] with two highly homologous albumins, HSA (human serum albumin) and BSA (bovine serum albumin), were investigated through CD, spectrofluorophotometry, and molecular docking analysis. The absence of any tryptophanyl residue in detemir makes albumin binding study possible by exclusive...
Lima Bean Trypsin Inhibitor (LBTI) is 83 residues monomeric protein of 9.0 KDa, consisting of six antiparallel β-strands and can undergo concentration dependant dimerization. We have tried to characterize folding intermediates of LBTI under equilibrium denaturation conditions. We have used various spectroscopic and microscopic techniques to underst...
Background:
Sodium fluorescein (SF) is a fluorescent tracer dye used extensively in diagnostic tools in the field of Ophthalmology, particularly in intravenous fluorescein angiography (IVFA). The binding of SF to human serum albumin (HSA) has been predicted by molecular docking and investigated by circular dichroism (CD) and fluorescence spectrosc...
The present study describes the purification and physicochemical and biochemical characterization of trypsin like protease from green seeded chickpea (Cicer arientum). The crude extract of Chickpea Trypsin (CpT) was obtained by homogenization followed by differential ammonium sulphate precipitation. The CpT was purified by Ion exchange chromatograp...
The importance of domain II in the molecular interaction of bovine serum albumin (BSA) with curcumin was investigated by fluorescence spectroscopy and molecular docking. At pH 7.4 BSA is in its native state. Domain III of BSA unfolds at pH 4.0, and domains I and III unfold in the presence of 5 M urea. Curcumin has a high quenching constant (K
SV ~...
Very few studies have been done to understand the effect of millimolar concentrations of chaotropes on protein structure. In our previous study we observed that the secondary and tertiary structure of human serum albumin (HSA) increases in the presence of 5 mmol/L urea. Micelle formation in amphoteric detergents increases in the presence of equival...
Equilibrium unfolding by guanidinium hydrochloride (GuHCl) and urea as well as evolutionary trends of two homologous albumins, pig serum albumin (PSA) and rabbit serum albumin (RSA), has been studied with circular dichroism, tryptophanyl fluorescence and bioinformatics. GuHCl cannot distinguish the contribution of electrostatic interactions to the...
Multiple sequence alignment of PvTRAg33.5 (Plasmodb ID: PVX_121897) with close tryptophan rich homologs present in Plasmodium species. The sequences of these proteins were retrieved from the Plasmodium database (www.plasmodb.org) and aligned using ClustalW software at http:/www.ebi.ac.uk/clustalW. The plasmodb ID of P.vivax homologs are PVX_090250...
Secondary structure prediction of exon 2 encoded PvTRAg33.5 by webservers and the consensus secondary structure prediction.
(TIF)
Secondary Structure Prediction Servers.
(DOC)
Tryptophan-rich proteins from several malarial parasites have been identified where they play an important role in host-parasite interaction. Structural characterization of these proteins is needed to develop them as therapeutic targets. Here, we describe a novel Plasmodium vivax tryptophan-rich protein named PvTRAg33.5. It is expressed by blood st...
Little work has been done to understand the folding of proteins at alkaline conditions. BSA acquires a partially reversible unfolded state at pH 13.0, devoid of any native structure. Introduction of methanol, ethanol and 2-propanol with the alkaline unfolded protein resulted in beta-sheet-like structure formation, and 2,2,2-trifluroethanol found to...
We have studied the effect of 2,2,2-trifluoroethanol (TFE), an α-helix inducer, versus methyl cyanide (MeCN), a β-sheet inducer,
on acid-denatured human serum albumin (HSA) using far-UV circular dichroism, intrinsic fluorescence, 1-anilino-8-naphthalene
sulfonate binding, and acrylamide quenching studies. Interestingly, at pH 2.0, where the recover...
Human serum albumin (HSA), being the most abundant carrier protein in blood and a modern day clinical tool for drug delivery, attracts high attention among biologists. Hence, its unfolding/refolding strategies and exogenous/endogenous ligand binding preference are of immense use in therapeutics and clinical biochemistry. Among its fellow proteins a...
The interactions among bovine, rabbit, and porcine serum albumins and single-chain cationic surfactant cetyltrimethylammonium bromide (CTAB) versus its gemini counterpart (designated as G4) have been studied. The studies were carried out in an aqueous medium at pH 7.0 using UV, intrinsic and extrinsic fluorescence spectroscopy, and far-UV circular...
The interaction of thioflavin T (ThT) with serum albumins from four different mammalian species i.e. human, bovine, porcine and rabbit, has been investigated by circular dichroism (CD), fluorescence spectroscopy and ITC. The binding constant (K) for HSA was found to be 9.9 x 10(4)M(-1), 4.3 x 10(4)M(-1) for RSA, 1.07 x 10(4)M(-1) for PSA and 0.3 x...
Colloids and Surfaces B: Biointerfaces j o u r n a l h o m e p a g e : w w w . e l s e v i e r . c o m / l o c a t e / c o l s u r f b Spectroscopic studies on the interaction of cationic surfactants with bovine serum albumin a b s t r a c t The interaction of the cationic surfactant cetyltrimethylammonium bromide (CTAB) with bovine serum albumin (...
Serum albumins are chief carrier of ligands in blood, hence important in clinical biotechnology. The effects of methyl cyanide (MeCN), a chief solvent of reverse phase chromatography, on four mammalian serum albumins (human, bovine, porcine and rabbit sources) were studied at neutral pH with the help of scattering, circular dichroism, IR and fluore...
The interaction of the cationic surfactant cetyltrimethylammonium bromide (CTAB) with bovine serum albumin (BSA), a globular protein, has been studied by small-angle neutron scattering (SANS), fluorescence and circular dichroism (CD). SANS measurements show that at low [CTAB] the protein shows a native-like behavior. On the other hand, at high [CTA...
To gain insights into the comparative effect of single-chain/gemini surfactants on proteins and the possible implications, the interaction of human serum albumin (HSA) with cationic single-chain surfactant cetyltrimethylammonium bromide (CTAB) and its gemini counterpart bis(cetyldimethylammonium)butane dibromide with spacer -(CH(2))(4)- (designated...
Little work has been done to understand the folding profiles of multi-domain proteins at alkaline conditions. We have found
the formation of a molten globule-like state in bovine serum albumin at pH 11.2 with the help of spectroscopic techniques;
like far and near ultra-violet circular dichroism, intrinsic and extrinsic fluorescence spectroscopy. I...
We have previously characterized an acid-unfolded (U(A)) state of Mucor miehei lipase at pH 2. The effect of 2,2,2-trifluoroethanol (TFE) and 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) resulted in characterization of molten-globule (MG) like states with beta-sheet secondary structure at 15% (v/v) TFE and 6% (v/v) HFIP. alpha-Helical states accumulate...
We report that the presence of very low concentrations (<0.1 M) of urea, a widely used chemical denaturant, induces structure
formation in the water-soluble globular protein human serum albumin (HSA) at pH 7. We have presented results suggesting an
almost 8% and 5% increase in α-helix in the presence of 10 mM urea (U) and 20 mM monomethylurea (MMU)...
The effect of low, medium, and high molecular weight poly(ethylene glycol) (e.g., PEG-400, -6000, and -20,000) on the structure of the acid unfolded state of unmodified stem bromelain (SB) obtained at pH 2.0 has been studied by various spectroscopic methods. The conformation of stem bromelain at pH 2.0 exhibits substantial loss of secondary structu...
Questions
Questions (14)
The double log graph of Stern-volmer equation is showing is best fitting with exponential increment. How can I explain it? what is its mathematical expression and interpretation? the linear equation is log[F0-F]/F= n log[Q]+logK. it gives the results like n (number of binding site) and K(Binding constant). What information the exponential graph will give?
Theoretically, my protein is unstable. till DNA level everything is quite fine, but I think I am losing it during heating with bromophenol for SDS-PAGE. Any suggestion?
I wish to know what are the major factor(s) in a snake venom(s) which causes the real harm and how?
Why 100mM Tris HCl buffer pH 7 freezes even 8-10 degree celcius?
It is told that mostly inclusion bodies possess only one protein. but why? why the kinetics of aggregation attracts only one protein?
