Priyanka Mishra

Illumina · Systems Engineering and Integration

Senna alexandrina (Fabaceae) is a globally recognized medicinal plant for its laxative properties as well as the only source of sennosides, and is highly exported bulk herb from India. Its major procurement is exclusively from limited cultivation, which leads to risks of deliberate or unintended adulteration. The market raw materials are in powdere...

The Orsay virus/C. elegans model system provides an opportunity to study receptors mediated innate immune pathways. Two pathways have been reported but in C. elegans only TOL-1 with other downstream pathway components viz. TRF-1, PIK-1, and IKB-1 exist. In fact, the cytoplasmic sensors receptors found only in human (HIN200/IFI16/AIM2) has not been...

The genetic model organism, Caenorhabditis elegans (C. elegans), shares many genes with humans and is the best-annotated of the eukaryotic genome. Therefore, the identification of new genes and pathways is unlikely. Nevertheless, host-pathogen interaction studies from viruses, recently discovered in the environment, has created new opportunity to d...

Identifying new molecular components and characterizing their function can reveal a novel innate immunity pathway and further provides better strategies to combat viral infection within organisms. The Orsay-Caenorhabditis elegans infection system offers an attractive possibility to identify these components since many genes are conserved with the h...

Caenorhabditis elegans infection by Orsay virus initiates a variety of abnormalities, including intestinal damages. However, the role of the viral capsid proteins to this pathogenicity needs to be further discovered. To address this question, engineered bacterial lawn expressing recombinant viral proteins were used as a food source to deliver them...

Gloriosa superba L. has economic significance due to colchicine, a bioactive compound used for gout. In present study metabolic and molecular variability in natural population of species was analyzed and correlated with edaphic and climatic factors. Thirty populations (wild) of G. superba were mapped from 10 different eco-regions of India at an ele...

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Presence of differences in the essential oil content, chromosome count and the morphology, led to consider Ocimum gratissimum as a polymorphic complex species often subcategorized into subspecies, varieties, and forms. Characterization of O. gratissimum complex is carried out using morphological, molecular markers combined with chemical analysis of...

Rubia cordifolia L., is an industrially viable medicinal crop and is widely exploited for the therapeutic potential of its bioactive metabolite, purpurin. The present investigation aimed to explore the chemotypic and molecular variability in seven wild populations of R. cordifolia from South Eastern Ghats region of India. Thirty-eight individuals w...

The steno-endemic species of genus Decalepis are highly threatened by destructive wild harvesting. The medicinally important fleshy tuberous roots of Decalepis hamiltonii are traded as substitute, to meet the international market demand of Hemidesmus indicus. In addition, the tuberous roots of all three species of Decalepis possess similar exudates...

DNA barcoding is used as a universal tool for delimiting species boundaries in taxonomically challenging groups, with different plastid and nuclear regions (rbcL, matK, ITS and psbAtrnH) being recommended as primary DNA barcodes for plants. We evaluated the feasibility of using these regions in the species-rich genus Terminalia, which exhibits vari...

Sample details and GenBank (NCBI) accession numbers of for all the samples of Terminalia corresponding to different regions used in this study. Accessions numbers marked in bold represent the sequences generated in our lab. (DOC)

ITS+matK sequences alignment (concatenated) used in the study. (FASTA)

rbcL sequences alignment used in the study. (FASTA)

matK sequences alignment used in the study. (FASTA)

ITS sequences alignment used in the study. (FASTA)

rbcL+matK+ITS sequences alignment (concatenated) used in the study. (FASTA)

rbcL+matK sequences alignment (concatenated) used in the study. (FASTA)

ITS+rbcL sequences alignment (concatenated) used in the study. (FASTA)

Premise of the Study. The internal transcribed spacer ( ITS ) region is situated between 18S and 26S in a polycistronic rRNA precursor transcript. It had been proved to be the most commonly sequenced region across plant species to resolve phylogenetic relationships ranging from shallow to deep taxonomic levels. Despite several taxonomical revisions...

Ocimum tenuiflorum has been widely used in traditional medicine and has high medicinal value. High volume trade of this potential medicinal plant species led to unscrupulous adulteration of both crude drugs as well as formulations. Morphology-based authentication is difficult in cases of incomplete or damaged samples and in dried herbal materials....

C. forskohlii (willd.) Briq. is an industrially viable medicinal crop and is widely exploited for the therapeutic potential of its bioactive metabolite, forskolin. The present investigation aimed to explore the chemotypic variability of forskolin content and existing molecular diversity in the wild population of C. forskohlii from the Western Himal...

Premise of the Study The internal transcribed spacer (ITS) region is situated between 18S and 26S in a polycistronic rRNA precursor transcript. It had been proved to be the most commonly sequenced region across plant species to resolve phylogenetic relationships ranging from shallow to deep taxonomic levels. Despite several taxonomical revisions in...

The aligned sequences matrix of ITS2

The aligned sequences matrix of ITS1

Concatenated aligned sequences matrix of ITS1+2

Phylogenetic consensus tree obtained using maximum likelihood algorithm Phylogenetic consensus tree obtained for Cassia, Senna, and Chamaecrista species based on nrITS datasets constructed using maximum likelihood algorithm.

Purpose: Globalization of trade is expanding the herbal product market. India with its immense biodiversity is sitting on a gold mine of well recorded and traditionally well-practised knowledge of herbal medicine which are the fastest-growing segment of the alternative medicines market world-wide. The dramatic increase in exports of medicinal plant...

High level of morphological as well as chemical variability exists within the genus Ocimum, and its taxonomy and phylogenetic relationships are still doubtful. For evaluating interspecific genetic relationships among the Ocimum species, genotyping with intersimple sequence repeat (ISSR) markers and sequence analyses of noncoding psbA-trnH intergeni...

The past couple of decades have witnessed global resurgence of herbal-based health care. As a result, the trade of raw drugs has surged globally. Accurate and fast scientific identification of the plant(s) is the key to success for the herbal drug industry. The conventional approach is to engage an expert taxonomist, who uses a mix of traditional a...

Compositional characteristics of the essential oils of four Ocimum spp., namely Ocimum adscendens Willd., Ocimum gratissimum L., Ocimum tenuiflorum L. and Ocimum americanum L. were examined using GC-FID, GC-MS, and hierarchical cluster analyses from peninsular India. The essential oil content varied from 1.0% to 2.0% in different Ocimum spp. Altoge...

Decalepis arayalpathra, a critically endangered plant species, has a restricted and fragmented population in Southern Western Ghats, India. This study is a first attempt to evaluate genetic diversity and population structure in the nine wild populations of D. arayalpathra based on molecular pattern realized through the marker assays. Principal coor...

The internal transcribed spacer (ITS) region of 18S-26S nuclear ribosomal DNA (nrDNA) has proven to be a useful source of characters for phylogenetic studies in many angiosperms. Family Combretaceae belonging to order Myrtales comprises of about 18 genera and 600 species with tropical distribution around the globe. The plants are highly valued for...

Decalepis arayalpathra, an endangered, endemic ethnomedicinal plant from southern Western Ghats, India, is targeted for its aromatic and medicinal properties. This study aimed at to identify fungal endophyte populations associated with healthy and diseased roots of this perennial shrub. Healthy and rotted root samples of D. arayalpathra were collec...

Decalepis arayalpathra (J. Joseph and V. Chandras.) Venter, which belongs to the family Apocynaceae, is a perennial under shrub, endemic to southern Western Ghats, India. The highly aromatic tuberous roots of the D. arayalpathra are used as an effective remedy for peptic ulcer, cancer-like afflictions and as rejuvenating tonic by native tribes. The...

Biological diversity or biodiversity includes diversity within species, between species and of ecosystems. A healthier biodiversity is of fundamental importance as it delivers a variety of natural services, viz., ecosystem services, biological resources and social benefits, completely free of charge to the human society. In the recent decades, inte...

Genetic variation within and among population is the basis for survival of the population both in short and long term. Thus, studying the plant genetic diversity is essential for any conservation program. Indigenous medicinal plants like Justicia adhatoda L. which are facing high rate of depletion from the wild population need immediate attention....

Seasonal variation of the essential oil of Eucalyptus citriodora leaves were analysed by Gas chromatography mass spectrometry (GC-MS) analysis in the trees grown at subtropical conditions of North Indian plains for commercial cultivation and determination of proper harvesting time. At this condition, oil yield ranged between 1.0 to 2.1% during diff...

Artemisia myriantha var. pleiocephala is a rare aromatic and medicinal plant. In India, it was reported from Western Himalaya between 1500-2000 m and Deccan peninsula. This plant is reported to possess anthelmintic, expectorant, antiseptic and insect repellent properties. Since, this is a rare plant, an attempt has been made to conserve, acclimatiz...

The natural population of several of our important medicinal tree species are depleting due to destructive collection of their drug parts like bark, root and wood. In constructing the sustainable world that we want in future, the intelligent use of biological diversity is of central importance. Therefore, these tree species require special attentio...

“Calliterpenone” a phyllocladane diterpenoids isolated from Callicarpa macrophylla has recently been reported not only to enhance the seed germination and production of bio-mass of roots, shoots and flowers in Angiosperms but also found to enhance the population of beneficial microorganisms. Presently, the effect of calliterpenone has been recorded...

Cancer is the term which includes many types of malignancy within the generalization of a number of basic and distinguishable varieties viz. carcinoma, sarcoma, melanoma, teratoma, leukemia, etc. Plant products have always been in use for the treatment and management of diseases akin or related with sign and symptoms of malignant diseases. Generall...