Premkumar Natraj- M.Sc. PhD
- Senior Researcher at Jeju National University
Premkumar Natraj
- M.Sc. PhD
- Senior Researcher at Jeju National University
About
28
Publications
9,757
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170
Citations
Introduction
Welcome to my ResearchGate profile! I'm Premkumar Natraj (Daniel), a Ph.D. candidate at the Department of Veterinary Toxicology, College of Veterinary Medicine, Jeju National University, South Korea. My research interests include the molecular mechanisms of obesity and insulin signaling pathways, focusing on identifying potential therapeutic targets and exploring the role of flavonoids in regulating lipid metabolism. Please feel free to check out my publications or get in touch with me.
Current institution
Additional affiliations
March 2017 - December 2017
Position
- Research Assistant
Description
- Self-powered non-enzymatic cholesterol sensor based on host-guest complexation. Nonenzymatic sensors based on solid-state devices have a greater advantage in biomolecule detection. In our present work, β-cyclodextrin functionalized graphene (β-CD/G) film-based device for detecting cholesterol has been demonstrated for the first time.
July 2016 - January 2017
Position
- Research Assistant
Description
- Role of Coenzyme Q10 in Attenauting Bromobenzene Induced Toxic Changes in Wistar Albino Rats. The present study was regulated to evaluate the protective role of Coenzyme Q10 against bromobenzene induced hepatotoxicity and nephrotoxicity in Female Wistar albino rats through several mechanisms in assertive entity
Education
July 2011 - May 2016
Publications
Publications (28)
Physicochemical characterization of CeO2NPs using UV-Visible, FTIR and XRD have confirmed the characteristic identity and fluorite phase of their crystalline nature. The interaction studies between cerium oxide nanoparticles (CeO2NPs) and HSA were investigated using fluorescence emission and synchronous spectra, UV-Visible absorbance, FTIR, FT-Rama...
Pneumococcal infection is one of the major causes of morbidity and mortality among children below 2 years of age in underdeveloped countries. Current study involves the screening and identification of potent inhibitors of the pneumococcal virulence factor pneumolysin. About 100 flavonoids were chosen from scientific literature and docked with pnuem...
In this study, we demonstrate an effective skin therapy device based on the plasma generated nitric oxide (NO) and needling techniques. The NO generator was constructed based on the dielectric barrier discharge plasma and combined with a commercial needling device. As a result, continueous flow of the NO could be supplied during needling treatment...
The number of men and women who suffer hair loss and/or hair thinning are increasing in accordance with changes in lifestyle and nutritional balance. Therefore, it is important to
develop new materials to prevent hair loss and promote hair growth.
Background
Sulforaphane (SFN) is an isothiocyanate compound present in cruciferous vegetables. Although the anti-inflammatory effects of SFN have been reported, the precise mechanism related to the inflammatory genes is poorly understood.
Objectives
This study examined the relationship between the anti-inflammatory effects of SFN and the different...
The aryl hydrocarbon receptor (AhR) plays a crucial role in obesity-associated inflammation and metabolic dysfunction. This study investigates the transcriptomic and structural effects of citrus-derived flavonoids—hesperidin (HES), narirutin (NAR), nobiletin (NOB), sinensetin (SIN), and tangeretin (TAN)—on AhR signaling in 3T3-L1 adipocytes. Adipoc...
Papaya (Carica papaya L.) is a vital tropical fruit crop valued for its nutritional, pharmacological, and industrial applications. However, its cultivation faces challenges, including genetic variability, susceptibility to diseases like papaya ringspot virus (PRSV), and limited propagation efficiency through traditional methods. Micropropagation ha...
This study examines the antioxidant capacity of papaya leaf extract (CPL) and its efficacy in reducing oxidative stress and inflammation in neuronal cells, which are key factors in neurodegenerative diseases. Through high-performance liquid chromatography coupled with diode-array detection, we quantified phenolic compounds present in CPL. Molecular...
Chayote (Sechium edule) has been traditionally used in Mexico to manage diabetes, owing to its hypoglycemic, anti‐oxidant, and anti‐inflammatory properties. However, the specific effects and mechanisms of chayote juice (SEJ) in preventing pancreatic dysfunction remain underexplored. This study investigated the pancreatic protective effects and unde...
This study analyzed the phytochemical composition and functional properties of leaves and green beans from seven Arabica coffee cultivars. The total phenolic and flavonoid contents were measured using spectrophotometric methods, while caffeine, chlorogenic acid (CGA), and mangiferin levels were quantified via High-Performance Liquid Chromatography...
Climate change, a pressing global concern, poses significant challenges to agricultural systems worldwide. Among the myriad impacts of climate change, the cultivation of kiwifruit trees (Actinidia spp.) faces multifaceted challenges. In this review, we delve into the intricate effects of climate change on kiwifruit production, which span phenologic...
Citrus fruits are rich in dietary flavonoids and have many health benefits, but their antiadipogenic mechanism of action and impact on lipid metabolism remains unclear. In this study, we investigated the effect of citrus flavonoids, namely, hesperidin (HES), narirutin (NAR), nobiletin (NOB), sinensetin (SIN), and tangeretin (TAN), on preventing fat ce...
Background
Cudrania tricuspidata is a perennial plant, and Sargassum fusiforme is a brown seaweed with numerous potential benefits, including anticancer, anti-inflammatory, and antioxidant activities. However, the efficacies of C. tricuspidata and S. fusiforme on hair growth have not yet been elucidated. Therefore, the present study examined the ef...
The present study examined the relationship between the anti-diabetic effect of hesperidin (HES) and the differential gene expression in HES treated high fat diet (HFD)-induced obese mice. Based on the glucose uptake assay, the treatment of HES restored the glucose uptake to control level in an insulin-independent manner in PA-treated HepG2 cells....
Background:
Obesity is a worldwide health threat. Premature citrus extract (PCE) are known to contain high concentrations of flavonoids, polyphenols, and organic acids with health functions. The current study aimed to investigate the anti-obesity effect of PCE on a high-fat diet (HFD)-induced obesity mice.
Method:
Mice were fed on chow or HFD wi...
Background:
Flavonoids are natural polyphenols found widely in citrus fruit and peel that possess anti-adipogenic effects. On the other hand, the detailed mechanisms for the anti-adipogenic effects of flavonoids are unclear.
Objectives:
The present study observed the anti-adipogenic effects of five major citrus flavonoids, including hesperidin (...
Background:
Naringin and its aglycone naringenin are citrus-derived flavonoids with several pharmacological effects. On the other hand, the mechanism for the anti-diabetic effects of naringenin and naringin are controversial and remain to be clarified further.
Objective:
This study examined the relationship between glucose uptake and AMP-activat...
The present study evaluated the anti-obesity effect of sulforaphane (SFN) and glucoraphanin (GRN) in broccoli leaf extract (BLE) on 3T3-L1 adipocytes and ob/ob mice. Based on Oil Red O staining and triglyceride (TG) assay, SFN and BLE significantly reduced (P < 0.05) both lipid accumulation and TG content in the differentiated 3T3-L1 adipocytes. SF...
The present study reveals the underlying mechanism of the anti-obesity effect of PCE, which can serve as a potential candidate for the management of obesity. In addition, our data showed that PCE activates the AMPK signalling pathway, thereby modulating energy metabolisms such as lipogenesis and lipolysis. In summarizing, these findings are helpful...
Background: Naringenin and its glycoside naringin are well known citrus flavonoids with
several therapeutic benefits. Although the anti-adipogenic effects of naringenin and naringin
have been reported previously, the detailed mechanism underlying their anti-adipogenesis
effects is poorly understood.
Objectives: This study examined the anti-adipogen...
The present study evaluated the anti-diabetic activity of broccoli leaf extract (BLE) in HepG2 cells and ob/ob mice. The analysis of glucose uptake showed that BLE increases 2-NBDG uptake to normal level in high glucose treated HepG2 cells. Based on Western blot analysis, insulin-provoked phosphorylation levels of both protein kinase B (Akt) and gl...
The present study was performed to evaluate the anti-obesity effect of broccoli leaf extract (BLE) in 3T3-L1 adipocyte and ob/ob mice. Based on Oil Red O staining and triglyceride (TG) assay, BLE significantly (P<0.005) reduced both lipid accumulation and TG content in the differentiated 3T3-L1 adipocyte. Glucose uptake analysis showed that BLE inc...
In conclusion, the results indicate that naringin and naringenin inhibit the Lipid and TG accumulation in 3T3 adipocyte and phosphorylate both AMPK and ACC.The flavonoids affects the lipid metabolism by regulating the gene expression in adipocyte. Furthermore, flavonoid treatment improves the glucose uptake by increasing the phosphorylation of Akt...
The present study examined the anti- diabetic and anti-obesity effects of hesperetin in HepG2 cells and in 3T3-L1 adipocytes . The analysis of glucose uptake showed that hesperetin treatment enhanced the glucose uptake dose dependently in both high glucose treated HepG2 cells and 3T3 L1adipocytes. Western blot analysis revealed that hesperetin sign...
1. Effect of UCE and its major flavonoids on Lipid Accumulation in HepG2 cells Abstract: The present study was conducted to evaluate the effect of a natural product, unripe citrus extract (UCE) and its major flavonoids (Hesperidin, Narirutin, Nobiletin, Sinensetin and Tangeretin) against insulin resistance and disorders of lipid metabolism in palmi...
Phyllanthus urinaria L. has been used for the anti-inflammatory drug in Chinese traditional medicine. The present study evaluated anti-oxidative, anti-bacterial and anti-inflammatory effects of Phyllanthus urinaria L. ethanol extract (PUE). The antibacterial activity of PUE was tested against Edwardsiella tarda (E. tarda), Streptococcus parauberis...
This study was conducted to examine the influence of Fully Flexible Credit System (FFCS) on the academic performance as well as the learning experience of students in VIT University, Vellore, India. The respondents in this study were randomly selected 100 students of VIT University. A survey was conducted by using a questionnaire through online sur...
Questions
Questions (7)
Dear Researchers,
I am performing a Western Blot analysis. My protein's expected molecular weight was 96-122 kDa, but I observed it at 60-75 kDa.
Is there any problem with my protein preparation or something?
I need to know the reason for the fluctuation in molecular weight.
We are doing research with C57BL/6 mice to induce obesity using High-fat diet (D12492-60% kcal% fat).
After 2-week observation, the high-fat diet intake mice have a wavy hair problem and also sample treatment group showed the same. We are curious without any sample treatment mice also having a wavy hair problem.
Please help me out! Is there any problem with the high-fat diet or not?
Dear researchers,
I am new to Western blot analysis. whenever I am trying to absorb the phosphorylation of ACC (Acetyl-CoA Carboxylase) detecting by Western blot, I always observed a double band of pACC.Even several research papers observed double band but none of them explained the reason. Herewith attached the pictures of my membrane which I observed phosphorylation of ACC. Please help me to identify the reason.
I estimated the protein concentration of unknown sample using standard curve but i want to know how to calculate amount of protein that has to be loaded into the SDS-PAGE gel. Please help me out
We are looking for functions of the above genes with reference .Please help me out.
What does metformin do to AKT, pGSK3-Beta and Glycogen synthase under insulin signaling ?
I tried DPPH in UV spec but i want to know about micro plate reader is suitable or not .
