Prem Swaroop Yadav

Prem Swaroop Yadav
Harvard Medical School | HMS · Division of Endocrinology

19.6
 · 
M.Sc. PhD

About

24
Publications
2,583
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156
Citations
Introduction
Prem Swaroop Yadav currently works at the Harvard Medical School, Harvard University. Prem does research in Developmental Biology, Cell Biology, Biotechnology and Mouse Genetics. He is currently studying the molecular regulation of embryonic bone and cartilage development.
Research Experience
September 2019 - present
Massachusetts General Hospital
Position
  • Researcher
July 2016 - September 2019
Harvard Medical School
Position
  • Post Doctoral Fellow
July 2009 - June 2016
Indian Institute of Technology Kanpur
Position
  • PhD

Publications

Publications (24)
Article
Full-text available
Mechanical forces are fundamental regulators of cell behaviors. However, molecular regulation of mechanotransduction remain poorly understood. Here we identified the mechanosensitive channels Piezo1 and Piezo2 as key force sensors required for bone development and osteoblast differentiation. Loss of Piezo1, or more severely Piezo1/2, in mesenchymal...
Article
Full-text available
Mechanical forces are fundamental regulators of cell behaviors. However, molecular regulation of mechanotransduction remain poorly understood. Here we identified the mechanosensitive channels Piezo1 and Piezo2 as key force sensors required for bone development and osteoblast differentiation. Loss of Piezo1, or more severely Piezo1/2, in mesenchymal...
Article
Full-text available
Mechanical forces are fundamental regulators of cell behaviors. However, molecular regulation of mechanotransduction remain poorly understood. Here, we identified the mechanosensitive channels Piezo1 and Piezo2 as key force sensors required for bone development and osteoblast differentiation. Loss of Piezo1, or more severely Piezo1/2, in mesenchyma...
Article
Full-text available
Mechanical forces are fundamental regulators of cell behaviors. However, molecular regulation of mechanotransduction remain poorly understood. Here, we identified the mechanosensitive channels Piezo1 and Piezo2 as key force sensors required for bone development and osteoblast differentiation. Loss of Piezo1, or more severely Piezo1/2, in mesenchyma...
Article
Full-text available
Fibrous dysplasia (FD; Online Mendelian Inheritance in Man no. 174800) is a crippling skeletal disease caused by activating mutations of the GNAS gene, which encodes the stimulatory G protein Gαs FD can lead to severe adverse conditions such as bone deformity, fracture, and severe pain, leading to functional impairment and wheelchair confinement. S...
Article
Bmp2 and Bmp4 genes were ablated in adult mice (KO) using a conditional gene knockout technology. Bones were evaluated by microcomputed tomography (μCT), bone strength tester, histomorphometry and serum biochemical markers of bone turnover. Drill-hole was made at femur metaphysis and bone regeneration in the hole site was measured by calcein bindin...
Article
Full-text available
Adipogenesis, chondrogenesis and osteogenesis are BMP signaling dependent differentiation processes. However, the molecular networks operating downstream of BMP signaling to bring about these distinct fates are yet to be fully elucidated. We have developed a novel Bone Marrow Stromal Cell (BMSC) derived mouse cell line as a powerful in vitro platfo...
Article
Bone marrow stromal cells (BMSCs) are a source of autologous stem cells that have the potential for undergoing differentiation into multiple cell types including neurons. Although the neuronal differentiation of mesenchymal stem cells has been studied for a long time, the molecular players involved are still not defined. Here we report that the gen...
Article
In vertebrates, BMP signaling has been demonstrated to be sufficient for bone formation in several tissue contexts. This suggests that genes necessary for bone formation are expressed in a BMP signaling dependent manner. However, till date no gene has been reported to be expressed in a BMP signaling dependent manner in bone. Our aim was to identify...
Article
Bone Morphogenetic Protein (BMP) signaling has been implicated in several processes during embryonic development and in adult tissue homeostasis. Maintenance of many organs such as skin, intestinal villi, bones and bone marrow requires continuous regeneration and subsequent differentiation of stem cells in order to maintain organ shape and size nec...
Article
Full-text available
BMP signaling pathway is critical for vertebrate development and tissue homeostasis. High-throughput molecular genetic screening may reveal novel players regulating BMP signaling response while chemical genetic screening of BMP signaling modifiers may have clinical significance. It is therefore important to generate a cell-based tool to execute suc...
Data
Description of primers used in this study. (DOC)
Data
4-OHT induced recombination in BRITER cell line. (A) Schematic showing the genomic configuration for Bmp2 and Bmp4 conditional alleles. The primers for genotyping are shown as red arrows. (B) Bmp2 and Bmp4 floxed alleles before and after recombination in absence and presence of 4-OHT, respectively. Amplicon for Cre transgene is used as loading cont...
Data
Time course of luciferase activity in C2C12 cells. C2C12 cells were transiently transfected with dual luciferase construct (pBFIR) and treated with 100 ng/ml BMP2 protein or vehicle. Dual luciferase assay was conducted after indicated duration of incubation (in hrs). Green line depicts normalized (with BMP2 untreated cell lysate values) Renilla luc...
Data
(A) BRE enhancer does not become sensitized due to prolonged exposure to recombinant BMP2 protein. Time course analysis showing relative FFLuc activity in presence of 100 ng/ml BMP2 concentrations at different time points indicated. For the “9+” hour time point sample recombinant BMP2 protein (100 ng/ml) was added at 0 hour time point and then agai...

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