Prem Prakash DasNational Research Council Canada | NRC · Aquatic and Crop Resource Development Research Area
Prem Prakash Das
PhD (National University of Singapore)
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Citations since 2017
14 Research Items
July 2019 - September 2021
- Research Fellow
- I am working on various proteomics projects related to plants and zebrafish at Protein and Proteomics Centre, DBS, NUS.
August 2015 - April 2017
January 2015 - April 2019
Pea (Pisum sativum) is one of the most abundant and sustainable alternate source of protein. Although pea proteins have good quantities of most of the essential amino acids, they have a limited supply of tryptophan, methionine and cysteine. Moreover, pea proteins have poor techno-functional properties compared to proteins from animal sources, limit...
Plant viruses trigger numerous responses in their insect vectors. Using iTRAQ-based quantitative proteomics analysis, early responses of the insect vector, the small brown planthopper (Laodelphax striatellus Fallén, SBPH), after acquiring Rice black-streaked dwarf virus (RBSDV) at 3 days and 5 days post first access to diseased plants (padp) were r...
Plants trigger a highly orchestrated defence mechanism in response to viral infection. In this study, we aimed at understanding the molecular events that lead to more accelerated cell death in Nicotiana benthamiana plants infected with the fast-replicating TMV 24A + UPD in comparison to TMV. TMV 24A + UPD is an artificial mutant that induces more s...
Heterologous superinfection exclusion (HSE) is a phenomenon of an initial virus infection which prevents reinfection by a distantly related or unrelated challenger virus strain in the same host. Here, we demonstrate that a mild strain mutant of Tobacco mosaic virus (TMV-43A) can protect Nicotiana benthamiana plants against infection by a challenger...
Background RADA-4 (Ac-RADARADARADARADA-NH2) is the most extensively studied and marketed self-assembling peptide, forming hydrogel, used to create defined threedimensional microenvironments for cell culture applications. Objectives In this work, we use various biophysical techniques to investigate the length dependency of RADA aggregation and asse...
Zebrafish is a well-recognized organism for investigating vertebrate development and human diseases. However, the data on zebrafish proteome are scarce, particularly during embryogenesis. This is mostly due to the overwhelming abundance of egg yolk proteins, which tend to mask the detectable presence of less abundant proteins. We developed an effic...
Background: Zebrafish is a well-recognised organism for investigating vertebrate development and human diseases. However, the data on zebrafish proteome are scarce, particularly during embryogenesis. This is mostly due to the overwhelming abundance of egg yolk proteins, which tend to mask the detectable presence of less abundant proteins. Results:...
In planta proximity-dependent biotin identification (BioID) identifies a TMV replication co-chaperone NbSGT1 in the vicinity of 126 kDa replicase
Zebrafish is a well-established model organism for the investigation of development of vertebrates. Multiple genomics and transcriptomics studies have been performed to analyse zebrafish embryonic development in recent years. However, quantitative proteomics studies of early embryo remained unexplored. Previous proteomics analysis of early embryo w...
Background: Autophagy is a conserved, highly-regulated catabolic process that plays important roles in growth, development and innate immunity in plants. In this study, we compared the rate of autophagy induction in Nicotiana benthamiana plants infected with Tobacco mosaic virus or the TMV 24A + UPD mutant variant, which replicates at a faster rat...
Zebrafish is a well-characterized model organism for the investigation of development of vertebrates. Proteome profile of zebrafish has high similarity to human, making it an attractive model for the study of human diseases. Genomic and transcriptomic resources on the development of zebrafish embryo are relatively well established, yet the proteomi...
Tobacco mosaic virus (TMV) is a positive, single-stranded RNA virus. It encodes two replicases (126 kDa and 183 kDa), a movement protein and a coat protein. These proteins interact with host proteins for successful infection. Some host proteins such as eEF1α, Tm-1, TOM1, 14-3-3 proteins directly interact with Tobamovirus replication proteins. There...
Cross-protection is a phenomenon in which infection with a mild virus strain protects host plants against subsequent infection with a closely related severe virus strain. This study showed that a mild strain mutant virus, Tobacco mosaic virus (TMV)-43A could cross protect Nicotiana benthamiana plants against wild-type TMV. Furthermore, we investiga...
Tobacco mosaic virus (TMV) is a positive single-stranded RNA virus. Its 5′ end ORF codes for the replicase proteins, namely 126 kDa and 183 kDa, respectively. These proteins interact with many host proteins to form a virus replication complex (VRC). This study aims to dissect the proteome profile of TMV-infected Nicotiana tabacum in host cellular a...
Comparative mitochondrial genomics of helminth parasites: An insilico approach Abstract Mitochondrial genomes have been sequenced from a wide variety of organisms, including an increasing number of parasites. They maintain some characteristics in common across the spectrum of life-a common core of genes related to mitochondrial respiration being...
A comparatively small fraction of nematodes are plant parasites that are important pests of crop plants worldwide, and also among the most difficult animals to identify. Their identification based on nuclear ribosomal DNA (rDNA) cistron and internal transcribed spacers (ITS1 and ITS2) is critical for structure prediction and phylogeny. Extensive st...
I expressed protein and unfortunately it became insoluble and remained in cell pellet. How do I get protein from pellet, so later I can use it for antibodies production?
I would like to express viral RdRP in E. Coli, so I can isolate RdRP protein.
To understand the host-pathogen interaction and identify unique host factors helps in virus replication process.