Praveen Kumar Vemuri

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• M.Tech, Ph.D
• Professor at Koneru Lakshmaiah Education Foundation

β-Lactoglobulin (β-LG), a major whey protein, is able to form amyloid fibrillar aggregates, when subjected to urea-induced denaturation at pH 7.0. Crocin, being a polar carotenoid, was used to investigate its influence on the urea-induced unfolding of β-LG and formation of amyloid fibrils. Crocin was found to stabilize β-LG structure against urea a...

The study aimed to isolate and characterize zinc ionophores from Terminalia bellirica fruit using a liposome assay and test its utility in H9c2 rat cardiomyoblasts cells subjected to hypoxia/reoxygenation. Ethyl acetate extract that exhibited zinc ionophore activity was resolved to yield three polyphenols that were characterized as epicatechin-3-ga...

Through targeted interactions mediated by receptors on antibodies, B-cells effectively neutralize invading pathogens by binding with remarkable precision to specific molecular regions, called epitopes. This study focuses on enhancing B-cell epitope predictions via computational methods. Antigen sequences are fragmented into peptides and docked agai...

Background: Doxorubicin (DOX) is a commonly utilized chemotherapeutic agent for treating hematological malignancies and solid tumors. However, its widespread use is hindered by the significant side effect of oxidative injury-related cardiotoxicity. Objective: This study explores the potential of procyanidins (PCNs) derived from Saraca asoca bark, k...

Ischemic heart disease is the leading cause of morbidity and mortality worldwide for cardiovascular illnesses, which continue to be a major public health problem. The effectiveness of conventional therapy in promoting heart regeneration is limited. A rapidly developing technology called bionanomaterials has great potential to meet this unmet medica...

To date, a variety of biological manufacturing techniques have been used in the synthesis of nanomaterials with the main goal of reducing their toxicity levels for both humans and the environment. This is the main element that has greatly aided in the conceptualization and evolution of bionanomaterials. Bionanomaterials, which are extraordinarily s...

Food is the primary cause for diseases in humans and carries high risk pathogens. Assessment of the safety in foods is needed to validate the presence of pathogenic bacteria. We used colony PCR for this approach to detect foodborne pathogens such as Escherichia coli, Lactobacillus and Bacillus cereus. Suitable primers were selected based on specifi...

Schizophrenia is a complex and debilitating mental disorder characterized by a range of symptoms, including hallucinations, delusions, and cognitive impairment. Early detection & prediction of schizophrenia is very important for stabilizing the condition. Normal machine learning approaches are facing few limitations like maintaining huge psychiatri...

The invention relates to a novel process for producing environmentally friendly bio- based fuels from diverse biomass feedstocks. Through optimized pretreatment, hydrolysis, fermentation, separation, and residue utilization techniques, this process efficiently converts renewable biomass resources into high-quality fuels like ethanol, butanol, or bi...

Introduction Enhanced aldose reductase activity results in increased accumulation of sorbitol. Therefore, inhibition of aldose reductase is an effective strategy to prevent or delay certain diabetic complications. Method Various extracts of the leaves of B. orellana were tested for their inhibitory activity on the aldose reductase. Ethyl acetate e...

The fundamental premise behind clinical approaches for dendritic cell mediated immunization in cancer is that the limiting defect in natural antitumor immunity is at the level of antigen presentation. In contrast to vaccines for the prevention of infections, cancer vaccines are administered in a therapeutic mode, to eradicate antigen-bearing tumor...

Background: The demand for tomato is increasing day by day mostly because of the increased per capita fresh fruit consumption. Nonetheless, as a perishable fruit crop, it has relatively short life after ripening thus experiences remarkable post-harvest losses. Aims: Our approach in this study is to evaluate the consistency and purity of seeds that...

Diabetes mellitus is a chronic disease that poses a serious global health problem, due to its associated effects on obesity and aging. Therapeutic strategies for targeting diabetes include the downregulation and/or inhibition of enzymes such as a-amylase and a-glucosidase, hydrolyzing the dietary carbohydrates in intestine. There is increasing inte...

Clinical and Pharmacological activity assessment of drugs

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, which first appeared in Chinese individuals in December 2019, is now causing the COVID-19 pandemic, with 5,79,319 deaths and 13,338,364 confirmed cases as of January 31st, a total of 56.7 lakhs. COVID-19 causes dysregulated immunological responses, metabolic dysfunctions, and n...

Due to the rising depletion of fossil fuels and increased energy demands, human society is looking for clean sustainable energy. Commercially produced algal biodiesel is limited by the expense and difficulty of oil extraction and subsequent biodiesel conversion technologies. Microalgae with high oil content are only alternatives for decreasing foss...

Background Bunyumwera virus can cause 82% mortality in humans currently with no vaccine or drugs for treatment. We described an in silico multi-epitope vaccine targeting Bunyumwera virus nucleocapsid N-protein and predicted B and T cell epitopes for immunogenicity, allergenicity, toxicity, and conservancy. For creating the most potent immunological...

Background: Pleurotus ostreatus, the oyster mushroom, is primarily an edible mushroom with high nutritional value and biomedical relevance, as it contains numerous bioactive components that generate the development of therapeutic effects. Aim: The purpose of this study is to determine the phytopharmacological effects of P. ostreatus. Material and M...

Increased accumulation of heavy metal ions such as Cr6+ and Co2+ due to release from prostheses and metallic implants has been reported. These metal ions have been shown to affect both resting and activated lymphocytes. Natural remedies towards mitigating the cytotoxic effects of metal ions are clearly warranted. Polyphenolic compounds which are pa...

The goal of this study is to perform a systematic assessment of the literature to determine the effectiveness and safety of molnupiravir and paxlovid oral antiviral drugs in COVID-19 patients. These Drugs has shown consistent efficacy against the SARS-CoV-2 virus's Gamma, Delta, and Mu versions, the firm has yet to release data on the drug's action...

Cancer is a disease caused by defective cells that have an uncontrollable ability to proliferate without regard for the physiological organ. Cancer is a complicated multi-factorial, multi-staged, and multi-mechanistic disease. Within the initiation and course of manifestation, it comprises the interaction of environmental and host elements. Inherit...

or Introduction Pleurotus ostreatus is primarily an edible mushroom with high nutritional value and biomedical relevance, as it contains numerous bioactive components that generate the development of therapeutic effects. The purpose of this study is to determine the phytopharmacological effects of Pleurotus ostreatus. FT-IR analysis revealed the pr...

Pollens are typically the primary reason for seasonal hypersensitivity caused in many people that are released by a hundred different species of plants for fertilization. Not all pollens are the same or have the same effect on human beings, there are those worse than others. The human body works out on a defence mechanism by creating certain reacti...

Plasmids are extremely valuable as a source of DNA and for use in biotechnology. Our research goal was to develop plasmids with extremely high copy numbers for lab-scale plasmid preparations. Using the beverage method simplifies the process of obtaining a large quantity of plasmid DNA for various applications. Diet Coke as a beverage demonstrated t...

Introduction: Sorghum propinquum has been used for a variety of ethnomedicinal purposes, including blood stimulation and purification, as well as body defense. Aim: The study aims to determine the hematological activity of Sorghum propinquum leaf base extracts. Methods: The immunological effect of the leaf base extract was evaluated using tests on...

Background: White birch and hazel allergens, namely Bet v1 and Cor a1 are known allergens, but their allergen specificity is not yet characterized. Objective: To map the antigenic determinants responsible for IgE binding utilizing in silico modelling and docking of the peptides against IgE antibody. Methods: The antigen sequences were cut into pept...

Background: Unlike chemical techniques, the combination of metal oxide nanoparticles utilizing plant concentrate is a promising choice. The purpose of this work was to synthesize magnesium oxide nanoparticles (MgO-NPs) utilizing heartwood aqueous extract of Pterocarpus marsupium. The heartwood extract of Pterocarpus marsupium is rich in polyphenol...

Cancer is a multi-stage, multi-mechanistic, multifactorial complex process that has excessive potential for excessive proliferation with no relation to the physiological organ. Inherited genetic inclinations contribute extensively to about 10 % of breast cancers and about 13 % of colon cancers incidences. In the industrialized countries, 7% of most...

Background: Regardless of the broad explore in the territory of glycobiology concerning structure and capacity of glycans, lectins and glycosylation forms, numerous viewpoints are still left unexplored. Aim: In this study, we analyzed the effect of shuttle vector on the secretion of human galectin recombinant protein. Methods: The galectin was expr...

Rapid development of technology, leads to new possibilities embracing in various traditional business sectors specifically Internet of Things (IoT) along with smart devices plays significant role for the development of health care centre. The technology of IoT transforms the landscape of healthcare, thereby posing higher requirement of resource man...

Cisplatin upregulate the intercellular adhesion molecule expression on the surface of endothelium, which in turn mediates enhanced infiltration by monocytes or leukocytes, resulting in endothelial dysfunction. Here we examined changes induced by andrographolide, a diterpenoid lactone isolated from Andrographis paniculata on endothelial cell activat...

With advances in modern pharmaceutical sciences, the processes of discovery and development of new therapeutic drugs have changed dramatically, resulted in an extraordinary improvement in potential prophylactic and therapeutic interventions. Patents for biologic agents first marketed in the early 1980s are now beginning to expire, paving the way fo...

Objective: The purpose of the study delineates the growth and plasmid stability of E. coli JM109 host system. Methods: Different concentrations of drugs, chemicals and various frequency of radiations were subjected to the host system to verify the colony forming units along with plasmid concentration and stability. Results: Among chemicals, acridin...

Work pressure and stress are the major issues which effects human health, mainly in case of pregnant women. The main purpose of this article is how Internet of things (IoT) and Artificial intelligence can help us to control the mortality rate. IoT allows healthcare professionals to be more attentive and to communicate proactively with patients. Now...

Myocardial zinc dyshomeostasis is associated with caspase-3 activation, ErbB2 degradation and apoptosis during hypoxia/reoxygenation. Zinc pyrithione replenishes intracellular zinc, suppresses caspase-3, augments ErbB2 levels and improves cell survival. We hypothesize that zinc is capable of modulating redox and endoplasmic reticulum (ER) stress in...

The present study aims to evaluate the possible association of PPP3R1 gene polymorphism (CnB 5I/5D) in clinical presentation of mental retardation (MR). A total of 100 children with idiopathic mental retardation in the age group of 5-16 years from both the sexes were recruited in the study considering age and sex matched healthy controls. Both case...

Hydrogel is a macromolecular gel constructed of a network of cross-linked polymer chains. Hydrogels are class of materials that can be tuned toward the subjected stimuli and can be modified to imitate the extracellular environment of the body which makes hydrogel worthy of being used in tissue regeneration, drug delivery, and other fields of scienc...

With advances in modern pharmaceutical sciences, the processes of discovery and development of new therapeutic drugs have changed dramatically, resulted in an extraordinary improvement in potential prophylactic and therapeutic interventions. Patents for biologic agents first marketed in the early 1980s are now beginning to expire, paving the way fo...

An extract from the Parthenium hysterophorus leaves were screened for phytochemical constituents and its activity towards IgG and IgY for allergic responses. Phytochemical screening revealed the presence of glycosides and proteins and the immunological responses were found for both IgY and IgG immunoglobulins. The purpose of the study was to charac...

An effective procedure for obtaining fragrance finished fabric based on cellulose and chitosan has been developed. The first step involves the formation of oxidized cellulose, which is able to form a schiff's base with the chitosan. In order to maintain the strength of fabric, a novel enzymatic method for oxidation of cellulose is adopted. In the s...

Book has been designed to provide a broad overview of the field of metabolic engineering and to show various problems have been solved by a mutagenic approach.

Animal cell culture has become an essential tool for the study of most biochemical and physiological processes and the use of large-scale animal cell culture has become increasingly important to the commercial production of specific compounds for the pharmaceutical industry.

Alternative medicine has become popular these days as it is gaining practices across the globe. Our studies aim to detect phytocompounds and biochemical activities of aqueous, dichloro methane, ethyl acetate and methanolic extracts of Terminalia chebula. Antioxidant, flurorescence and anitmicrobial studies were carried out for the phytocompounds. M...

Objective: The purpose of the study delineates the growth and plasmid stability of E. coli DH5α host system. Materials and Methods: Different concentrations of drugs, chemicals, and various frequency of radiations were subjected to the host system to verify the colony-forming units along with plasmid concentration and stability. Results: Among chem...

Background: Allergic reactions induced by ingestion of foods containing sesame seeds are a well recognized cause of severe food-induced anaphylaxis. Aim: The objective of the present study was to isolate, purify and identify allergenic proteins of sesame seeds. Methods: Extract was obtained by use of isoelectric precipitation, with 96.7% protein co...

The fundamental premise behind clinical approaches for dendritic cell-mediated immunization in cancer is that the limiting defect in natural antitumor immunity is at the level of antigen presentation. In contrast to vaccines for the prevention of infections, cancer vaccines are administered in a therapeutic mode, to eradicate antigen-bearing tumor...

Higher eukaryotes possesses a large genome with a high level of gene sequence identity from other genomic DNA and is therefore difficult in assessment and time-consuming. Here we report on an efficient approach for rapid isolation and amplification of HOXD9 loci of human genome via touchdown PCR using semi degenerate primers. For the semi degenerat...

Cancer became a big question for scientific community as no existing treatments could solve the problems related to this dreadful disease. Research is in well progress since half century but it failed to give a right solution to fight against it. However the developments in science and technology facilitated scientists to develop new methods of tre...

Copper and cisplatin share copper transporter 1 (Ctr1) for cellular import. Copper depletion increases sensitivity of wild type yeast to cisplatin, whereas mitochondrial DNA-deficient rho0 cells are resistant to cisplatin. In the current study, we sought to determine whether copper deprivation modulates sensitivity of rho0 yeast to cisplatin. Yeast...

L-asparaginase (L-asparagine aminohydrolase) is an enzyme which was clinically proved as an antitumor agent to treat acute lymphoblastic leukemia. It catalyzes L-asparagine hydrolysis to L-aspartate and ammonia, and the depletion of asparagine causes cytotoxicity to leukemic cells. Microbial L-asparaginase (ASNase) production has attracted good att...

Objectives: The purpose of this study was to determine if zinc homeostasis is affected during ischemia/ reperfusion, if so, whether zinc pyrithione limits myocardial cell death and improves hemodynamics when administered as an adjunct to reperfusion and if ErbB receptor tyrosine kinases that are important for the long-term structural integrity of t...

Parthenium hysterophorus has widely reported traditional uses which include its use for stimulation and purification of platelets as well as body defense. Experiments were carried out to assess the impact of Parthenium hysterophorus leaf extract on hematological parameters of wistar rats. The results revealed that the extract (10–100 mg/kg p.o.) pr...

Platinum-based chemotherapeutic regimen induces vascular dysfunction. Action of cisplatin on endothelial cells is mediated by protein kinase C (PKC-α), which further activates nuclear factor-κB (NF-κB) and induces canonical transient receptor potential channel (TRPC1) and intercellular adhesion molecule (ICAM-1) expression. Increased ICAM-1 contrib...

Introduction: To insight the importance of immunoglobulin’s role in allergic reactions and to determine the mechanism of IgG in allergic responses. Aim: This study aimed at purifying the proteins from Parthenium hysterophorus flower and to check their allergic activity with IgG. Materials and Methods: Purification methods of phenol extraction for P...

Introduction: Galectin-3 expression act as a potential novel therapeutic strategy in the treatment of a broad range of inflammatory diseases. Aim: To study the cross talk between macrophage and adipocyte cells with galectin-3 upon LPS challenge. Materials and Methods: Cytokine secretion in 3T3-L1 adipocyte-RAW 264.7 macrophage cells were greatly en...

Pollinosis is one of the most common allergic diseases in Asia, Central and Northern Europe as well as in North America. Several proteins in parthenium pollen have already been characterized as allergens causing sensitization and the development of allergic symptoms in atopic individuals. Glutathione-S-transferase are a phylogenetically widespread...

The objective of this study was to identify the difference in protein profile of dry fruits and its concentration level in response to variance in rpm and time. Cashew, Dates and Currant were selected and prepared as smoothie using phosphate buffer saline and distilled water. Time based and varying rpm levels studies were also carried out followed...

Objective: Although a few techniques exist for recovery of deoxyribonucleic acid (DNA) from agarose gels, they generally require expensive equipment, extended elution times, or considerable handling of the sample after elution. The objective of this study is to determine the effective conventional method for maximum recovery of DNA from agarose gel...

The human Glucokinase gene encodes an enzyme called Glucokinase, which plays key role in Glucose homeostasis. Many single nucleotide polymorphisms found in Glucokinase (GCK) gene have been associated with various disorders including, hyperglycemia, obesity and Neonatal Diabetes Mellitus. In this study, we performed a comprehensive analysis of funct...

Background: Radix valerian is a natural preparation of the root and rhizome of the perennial herb utilized primarily in the treatment of insomnia and sleep disturbances. Quality control of valerian represents a major concern in the pharmaceutical industry due to the huge variety of these species and their almost daily new scientific findings. Objec...

The study was aimed to investigate which orthologues of Parthenium hysterophorus genes might encode the P20 protein. Polyethylene glycol fractionation of Parthenium hysterophorus leaf proteins has shown detection of low-abundance proteins. Samples incubated at -80°C also reduced precipitation time of protein to two hours. Further, sonication was us...

Considering the augmented importance of immunoglobulin’s role in allergic reactions and the underlying mechanisms of IgY in allergic conditions, the objective of our study is to purify proteins from Parthenium hysterophorus leaves, IgY antibodies from chicken eggs and to check the immunological activity of the antibodies with Parthenium hysterophor...

Objective: The rationale of the present research work is to get insights of galectin-3 function in modulating inflammation in macrophages and adipocytes culture systems. Methods: Recombinant galectin-3 was prepared and anti-inflammatory effect of galectin-3 were studied in 3T3-L adipocytes and RAW264.7 macrophages stimulated with lipopolysacchari...

Carbohydrates are traditionally considered to be an important source of energy for living organisms. Galectins are a group of proteins that bind to a class of carbohydrates known as beta-galactosides. Galectins play an important and complex role in intracellular pathways and disease mechanisms. Since 1994, scientists working in the field of carbohy...

A novel lectin has been isolated from Ocimum sanctum and purified to homogeneity by gel filtration chromatography, which eluted as a single symmetrical peak from a Biogel P-100 column with a molecular mass of 66 kDa. The lectin had a special agglutinating activity with human erythrocytes at a minimum concentration of 0.7 ug/ml. The lectin was stabl...

Heart tissue becomes zinc-depleted and the capacity to mobilize labile zinc is diminished, indicating zinc dyshomeostasis during ischemia/reperfusion (I/R). Apparently, zinc pyrithione restores the basal zinc levels during I/R and prevents apoptosis by activating phosphatidyl inositol-3-kinase/Akt and targeting mitochondrial permeability transition...

Modern biological tools of recombinant technology have tremendous potential to characterize DNA molecules with the choice of DNA ladders. Determination of molecular weight of linear and double stranded DNA fragments either on agarose or polyacrylamide gel is done extensively with the DNA ladders. The most popular DNA ladders available in the market...

Galectin-3, a unique member of the growing family of β-galactoside binding lectins, contains a single carbohydrate recognition domain and a glycine rich N-terminal domain, through which it can form oligomers and functions to cross-link both carbohydrate and non-carbohydrate ligands. Galectin-3 is widely expressed in adult tissues, secreted by macro...

Background: Over the past century, the areas of genomics, proteomics and lipids have captured the attention of investigators worldwide. Carbohydrates, have recently received increased attention through the expanding field of glycobiology; probably because they are very complex and not encoded in the genome. Objectives: The purpose of this study wa...

Probiotics improves the values of human life since the intestinal tract is a diverse microenvironment where more than 500 species of bacteria thrive. A single layer of epithelium is all that separates these microorganisms and pathogens from the underlying immune cells and thus epithelial barrier function is a key component in the defense mechanisms...

The growing diffusion of monoclonal and polyclonal antibodies in immunotherapy and immuno- diagnostics, and the strict standards for animal-care, has led to a renewed interest in the use of chickens for antibody production, as opposed to mammals. Egg yolk represents an economical source of polyclonal antibodies, since the amount of immunoglobulins...

Diesel oil-degrading bacterium Acinetobacter species was isolated from diesel oil-polluted soil samples from various locations of Autonagar, Vijayawada City, Andhra Pradesh, India. Two different strains having specific ability to degrade and utilize diesel oil for their growth were isolated. The isolated bacterium was identified as Acinetobacter sp...

The objective of this paper was to report the isolation of flourine degrading bacteria from the soil samples of different areas of Prakasam district. Two different strains having specific ability to degrade and utilize flourine or their growth were isolated. The optical pH and temperature of the selected bacterial isolates were 7 and 37 C, respecti...

Obesity and its associated metabolic pathologies are the most common metabolic diseases. A key emerging feature of obesity is the relationship between obesity and chronic inflammation, with increased cytokine production and acute-phase inflammatory signaling in the adipose tissue. For this reason, adipose tissue is no longer considered an inert dep...

Phosphatase serves several functions in plant metabolism including growth governance, phosphorous level control, starch breakdown etc. Alkaline phosphatases, acting at an alkaline pH 8, are a significant class of enzymes that catalyze release of phosphate esters especially. This enzyme study is so far limited only to animal source and partly to mic...

The growing diffusion of monoclonal and polyclonal antibodies in immunotherapy and immuno-diagnostics, and the strict standards for animal-care, has led to a renewed interest in the use of chickens for antibody production, as opposed to mammals. Egg yolk represents an economical source of polyclonal antibodies, since the amount of immunoglobulins (...

Rice production is constrained by diseases of fungal, bacterial and viral origin. In this paper, we have discussed the various pathological symptoms in Oryza sps. Among them, Xanthomonas oryzae, Magnaporthe grisea, Rice Yellow Mottle Virus (RYMV), and brown planthopper cause high yield losses. Usage of pesticides is costly and environmentally undes...

Cancer became a big question for scientific community as no existing treatments could solve the problems related to this dreadful disease. Research is in well progress since half century but it failed to give a right solution to fight against it. However the developments in science and technology facilitated scientists to develop new methods of tre...

Immunologic approaches to cancer therapy rely on two distinct capabilities of the immune system: targeting the tumour microenvironment by recognising molecules expressed to a greater extent on tumour cells than normal cells and generating immune responses that can kill tumour cells. Immunocytokines, which are fusion proteins composed of a recombina...

The objective of this study is to obtain and characterize thermostable alkaline phosphatase from plant seeds than produced prior from bacterial and animal sources. Alkaline phosphatase was isolated and purified from plant seedlings by a streamline method without the use of proteolytic and lipolytic enzymes, butanol, or heavy metals. The study featu...

Cancer became a big question for scientific community as no existing treatments could solve the problems related to this dreadful disease. Research is in well progress since half century but it failed to give a right solution to fight against it. However the developments in science and technology facilitated scientists to develop new methods of tre...

Immunoglobulins are γ- globulin proteins secreted by B cells that are found in blood or other bodily fluids of vertebrates which have the basic structural units each with two large heavy chains and two small light chains. Five different immunoglobulin isotopes are known in mammals, which perform different roles, and help to direct the appropriate i...

Vegetables, fruits, plant and animal products contain DNA in various amounts. DNA, the carrier of genetic information, the recipes or blueprints of an organism, is made up of four different chemical units. These can be digested and used just like any other nutrient in our food. Usually DNA is degraded during cooking, but even if DNA is eaten uncook...

Resonance-like biological effects of microwave radiation at frequencies of approximately 50 KHz on the growth rate of E. coli and on DNA have been reported in several scientific publications. In order to explore these non-thermal effects, we have measured the growth rate and the absorption spectrum of E. coli, irradiated by microwaves in the freque...

Production of alkaline protease employing the laboratory isolate, Bacillus sp. under solid state fermentation (SSF) was optimized. The effect of wheat bran was examined. The appropriate Incubation time, Temperature, pH, Moisture content, Inoculums size, Surfactant and mutagen effect were determined. Maximum yields of enzyme were achieved by employi...