Prateep Duengkae

• Doctor of Philosophy
• Professor (Assoc.Prof.) at Kasetsart University

Ecosystems are being disrupted by climate change and habitat fragmentation, which affect species survival through altered mating, feeding, and migration patterns. Mae Hong Son Province, Thailand, harbors a unique hydrological network that supports rich freshwater fish biodiversity. Blue mahseer (Neolissochilus stracheyi), which is restricted to hea...

Climate change has driven global temperature increases, resulting in severe heat waves, altered precipitation patterns, and extreme weather events. In April 2024, a massive die‐off of hybrid catfish larvae and fry occurred in Thai aquaculture farms, coinciding with elevated temperatures of air (37–41°C) and freshwater (30–32°C). This study aimed to...

The Bighead Catfish ( Clarias macrocephalus ) plays crucial roles in the ecosystem and as a protein source for humans in Nong Han Lake, Thailand, but their ecological and population dynamics, along with their genetic diversity and structure, are poorly understood. Therefore, the dynamics of genetic diversity and differentiation of bighead catfish p...

Passer domesticus holds the title of being the most widely distributed bird species globally, originating from Central-East Asia and spreading extensively due to human-mediated activities. While initially established as a native species about 10,000 years ago, their association with human-modified habitats has accelerated their expansion, particula...

The invasion of blackchin tilapia in Thailand is a critical ecological threat affecting native biodiversity and destabilizing ecosystems. It is also resulting in significant ecological and socio-economic impacts. Originally from West Africa and introduced globally for aquaculture, blackchin tilapia thrive in Thailand’s brackish and freshwater ecosy...

Background/Objectives: Accipitridae mitogenomes exhibit unique structural variations, including duplicated control regions (CRs) that undergo gradual degeneration into pseudo-CRs, revealing a complex evolutionary landscape. However, annotation of this characteristic in a subset of accipitrid genomes is lacking. Due to the taxonomic diversity of Acc...

Article Info Importance of the work: The fishing cat (Prionailurus viverrinus Bennett, 1833) is a poorly studied wildcat that inhabits floodplains and wetlands. It is threatened throughout its entire range and has even been extirpated in some locales due to human-driven habitat loss. Objectives: To use the maximum entropy algorithm to assess the en...

Fighting cock breeds have considerable historical and cultural place in Thailand. Breeds such as Lueng Hang Khao (LHK) and Pradu Hang Dam (PDH) are known for their impressive plumage and unique meat quality, suggesting selection for fighting and other purposes. However, information regarding the genetic diversity and clustering in indigenous and lo...

This study introduces a novel method for identifying individual Siamese crocodiles (Crocodylus siamensis), which is a crucial requirement for conservation and sustainable industry practices. Although deep metric learning (DML) has improved identification model robustness and reduced dependency on large datasets, comprehensive field studies and long...

Microplastics (MPs) are emerging as ubiquitous environmental pollutants worldwide. However, no research has been conducted in freshwater ecosystems within Thai protected areas (PAs), where biodiversity and natural resources are safeguarded for human well-being. The aim of this study was to explore the occurrence and abundance of potential MPs in th...

Over hundreds of years, cats have been domesticated and selectively bred, resulting in numerous pedigreed breeds expedited by recent cat shows and breeding associations. Concerns have been raised about the limited breeding options and the genetic implications of inbreeding, indicating challenges in maintaining genetic diversity and accurate identif...

The northern white-cheeked gibbon (Nomascus leucogenys) is native species of Southeast Asia, found in Laos, Vietnam, and China. N. leucogenys is critically endangered on the IUCN Red List. This study aimed to evaluate and predict its potential distribution within Laos under current climate conditions (2022) and future scenarios for 2050 and 2070 us...

Protected areas are designated to conserve biodiversity and provide a safe habitat for wildlife. This study aims to investigate the factors affecting elephant appearance and changes in habitat. The MaxEnt program was used to analyze data derived from the patrolling system with constant quality monitoring. Four categories of environmental factors, c...

Plastic pollution is a widespread and growing concern due to its transformation into microplastics (MPs), which can harm organisms and ecosystems. This study, aimed to identify plastic pollution in the feces of terrestrial vertebrates using convenience sampling both inside and outside protected areas in Western Thailand. We hypothesized that MPs ar...

Black kite Milvus migrans govinda is currently classified as a sedentary species in Thailand. Its ecology and seasonal movement have never been studied in the country. Four Black kites, two each of juvenile and adult, were tracked with a GPS-GSM logger weighing 14 and 22 g (Druid Technology Co., LTD). Juvenile kites were tagged when they were nestl...

Originating in Thailand, the Thai Ridgeback dog is known for its unique fur ridge that grows in the opposite direction along its back. Selective breeding and a limited populations in Thailand have led to significant close inbreeding among related individuals. The current Thai Ridgeback population is assumed to have experienced a loss of genetic div...

Plastic pollution in terrestrial wildlife represents a new conservation challenge, with research in this area, especially within protected areas (PAs), being scant. This study documents the accumulation of microplastics (MPs) in terrestrial wildlife both inside and outside PAs in western Thailand. Carcasses of road-killed vertebrates in good condit...

Plastic pollution in terrestrial wildlife represents a new conservation challenge, with research in this area, especially within protected areas (PAs), being scant. This study documents the accumulation of microplastics (MPs) in terrestrial wildlife both inside and outside PAs in western Thailand. Carcasses of road-killed vertebrates in good condit...

The North African catfish (Clarias gariepinus) is a significant species in aquaculture, which is crucial for ensuring food and nutrition security. Their high adaptability to diverse environments has led to an increase in the number of farms that are available for their production. However, long-term closed breeding adversely affects their reproduct...

The Bangkaew dog is an indigenous dog breed in the Phitsanulok province of Thailand. This breed is recognized by the Fédération Cynologique Internationale (FCI), a global canine organization. The unique traits of the Bangkaew breed lead to purebred selection for breeding, while only their traits and pedigree from parental history are recorded. Dete...

This study comprehensively reviewed various aspects of population status, distribution, conflict situations, and conservation strategies for managing human-elephant conflicts (HECs). The primary goal is to foster a clear understanding of the current status, issues, and problem-solving approaches to benefit elephant conservation and management. The...

The thermal stress caused by global climate change adversely affects the welfare, productivity, and reproductive performance of farm animals, including chickens, and causes substantial economic losses. However, the understanding of the genetic basis of the indigenous chicken adaptation to high ambient temperatures is limited. Hence, to reveal the g...

Understanding the spatial ecology of an animal is crucial for its conservation and management, particularly for threatened species. The gaur (Bos gaurus), globally classified as vulnerable, is distributed across mainland South and Southeast Asia. In Thailand, the overall gaur population is decreasing, and suitable habitats are declining. Conversely...

Monitoring on abundance and distribution of Water monitors in an area of urban, changing land use, In the Kasetsart University Bangkhen Campus. The survey was conducted three times between July and November in 2012, 2018 and 2021. Use 2 survey methods were used transects and live traps to implant a microchip (PIT tag) on water monitors in the study...

Article Info Importance of the work: The northern white-cheeked gibbon (Nomascus leucogenys) is classified as Critically Endangered on the IUCN red list, urgent species conservation is required. However, there remains a notable lack of information regarding gibbon population sizes, distribution and conservation efforts, particularly in regions with...

Silver barb (Barbonymus gonionotus) is among the most economically important freshwater fish species in Thailand. It ranks fourth in economic value and third in production weight for fisheries and culture in Thailand. An XX/XY sex-determination system based on gynogenesis was previously reported for this fish. In this study, the molecular basis und...

Bats are reservoirs for various pathogens, including SARS-like coronaviruses (CoVs). Understanding the distribution of bat species is crucial to identifying areas where viral spillover from bats to other animals or humans might occur. In this study, we performed species distribution modeling to predict suitable habitats within Thailand under curren...

Large-spotted Civet, Viverra megaspila (Blyth, 1862), is an endangered medium-sized carnivore. It has been reported for the first time in Kaeng Krachan National Park, Thailand. It is one of the least known species with only handful records from Thailand. It was previously recorded from Khao Ang Rue Nai Wildlife Sanctuary and Khao Yai National Park,...

Eukaryotes have varying numbers and structures of characteristic chromosomes across lineages or species. The evolutionary trajectory of species may have been affected by spontaneous genome rearrangements. Chromosome fusion drastically alters karyotypes. However, the mechanisms and consequences of chromosome fusions, particularly in muntjac species,...

In nucleotide public repositories, studies discovered data errors which resulted in incorrect species identification of several accipitrid raptors considered for conservation. Mislabeling, particularly in cases of cryptic species complexes and closely related species, which were identified based on morphological characteristics, was discovered. Pri...

The location of female-specific/linked loci identified in Siamese cobra (Naja kaouthia) previously has been determined through in silico chromosome mapping of the Indian cobra genome (N. naja) as a reference genome. In the present study, we used in silico chromosome mapping to identify sex-specific and linked loci in Siamese cobra. Many sex-specifi...

Hybrids between the critically endangered Siamese crocodile (Crocodylus siamensis) and least-concern saltwater crocodile (C. porosus) in captive populations represent a serious challenge for conservation and reintroduction programs due to the impact of anthropogenic activities. A previous study used microsatellite and mitochondrial DNA data to esta...

Large and meso-carnivores play crucial roles in ecosystems. A systematic camera trap study conducted in Khao Yai National Park has confirmed that this area serves as a habitat for at least 18 significant carnivorous wildlife species from 6 families and 14 genera. Most of this wildlife is active during the night. The study identified 2 species as en...

Lao Pa Koi (LPK) chicken is a popular fighting breed in Thailand, prized for (its unique characteristics acquired by selective breeding), and a valuable model for exploring the genetic diversity and admixture of red junglefowls and domestic chickens. In this study, genetic structure and diversity of LPK chicken were assessed using 28 microsatellite...

Microsatellites are short tandem DNA repeats, ubiquitous in genomes. They are believed to be under selection pressure, considering their high distribution and abundance beyond chance or random accumulation. However, limited analysis of microsatellites in single taxonomic groups makes it challenging to understand their evolutionary significance acro...

DNA barcoding without assessing reliability and validity causes taxonomic errors of species identification, which is responsible for disruptions of their conservation and aquaculture industry. Although DNA barcoding facilitates molecular identification and phylo-genetic analysis of species, its availability in clariid catfish lineage remains uncert...

Jade perch (Scortum barcoo) has an XX/XY sex-determination system (SDS); however, its sex chromosomes and sex-determining region remain unknown. The recent availability of the jade perch chromosome-level genomic data provides a valuable resource for pinpointing the location of functional genes and the whole genomic structure. In this study, we cond...

Microsatellites are polymorphic and cost-effective. Optimizing reduced microsatellite panels using heuristic algorithms eases budget constraints in genetic diversity and population genetic assessments. Microsatellite marker efficiency is strongly associated with its polymorphism and is quantified as the polymorphic information content (PIC). Nevert...

Understanding the genetic diversity of domestic chicken breeds under the impact of socio–cultural and ecological dynamics is vital for the conservation of natural resources. Mae Hong Son chicken is a local breed of North Thai domestic chicken widely distributed in Mae Hong Son Province, Thailand; however, its genetic characterization, origin, and d...

Knowledge of the genetic characteristics, origin, and local adaptation of chickens is essential to identify the traits required for chicken breeding programs. Chee Fah and Fah Luang are black-boned chicken breeds reared in Chiang Rai, Thailand. Chickens are an important part of the local economy and socio-culture; however, the genetic diversity, ch...

Nanorana yunnanensis has been reported in Thailand for many years; however, no specimens have been verifiably described. To verify the identity of this species in Thailand, we observed Thai specimens formerly assigned to the species N. bourreti and N. yunnanensis. The 12S rRNA, 16S rRNA, and ND2 mitochondrial genes were used to clarify the taxonomi...

Catfish make up a large group of widely distributed freshwater fish. Clariid catfish (Clariidae) are the most widespread freshwater fish species in Africa and Asia. They are adaptable and can survive out of water for considerable periods if they remain moist. Catfish have evolved auxiliary breathing organs to survive in challenging environments, su...

Populations of Siamese crocodiles (Crocodylus siamensis) have severely declined because of hunting and habitat fragmentation, necessitating a reintroduction plan involving commercial captive-bred populations. However, hybridization between Siamese and saltwater crocodiles (C. porosus) has occurred in captivity. Siamese crocodiles commonly have post...

Article Info Importance of the work: Urban ecosystems are among the most polluted due to anthropogenic development, with humans and wildlife being affected by this pollution. Objectives: To examine the presence of some birds and their correlation to pollution in the kind of anthropogenic noise and particulate matter (PM). Materials & Methods: Sixty...

Developing successful conservation programs for genetically depleted species is challenging. Survival and adaptive potential are related to genetic and habitat factors; therefore, conservation programs are designed to minimize risks associated with inbreeding and loss of genetic diversity. The greater mouse-deer (Tragulus napu) is a true forest spe...

This study used results from camera traps set for Dhole (Cuon alpinus) and their prey during 2017-2020 in Khao Yai National Park to assess the species richness of ground-dwelling birds and identify abundance and also temporal appearance of the species in the area. We selected only the Pheasant species that typically forage on the ground to investig...

Mitochondrial displacement loop (mt D-loop) sequence analyses have greatly improved assessments of genetic diversity, structure, and population dynamics of endangered species threatened by climate change and habitat loss. Tracking population haplotypes of these species using mitochondrial-based markers has opened new avenues for conservation genomi...

Article Info Importance of the work: Northwestern Thailand lacks any intensive survey of amphibian fauna; thus, this area is suspected to have undiscovered populations of amphibians. Therefore, conducting field surveys in such areas is essential to fill knowledge gaps about species richness in this animal group. Objectives: To describe a new specie...

Background The number of nucleotide sequences in public repositories has exploded recently. However, the data contain errors, leading to incorrect species identification. Several fighting fish (Betta spp.) are poorly described, with unresolved cryptic species complexes masking undescribed species. Here, DNA barcoding was used to detect erroneous se...

Chanachai Y, Nathalang A, Duengkae P, Sukmasuang R. 2022. Species diversity, abundance, and movement of small mammals in the dry evergreen forest at Khao Yai National Park, Thailand. Biodiversitas 23: 5892-5901. Small mammals play an integral role in the forest ecosystem. This research was conducted on The Mo Singto Forest Dynamics Plot, Khao Yai N...

Illegal wildlife trade is a major threat to global biodiversity. Asian elephants (Elephas maximus) are highly valued by various cultures as religious symbols and tourist attractions, which has led to a high demand for captive elephants. Owing to the unviability of captive breeding programs, several captive elephant populations are maintained by ill...

first_pagesettingsOrder Article Reprints Open AccessArticle Standard Identification Certificate for Legal Legislation of a Unique Gene Pool of Thai Domestic Elephants Originating from a Male Elephant Contribution to Breeding by Nattakan Ariyaraphong 1,2,3,†,Dung Ho My Nguyen 1,2,3,†,Worapong Singchat 1,3,‡,Warong Suksavate 1,3,Thitipong Panthum 1,3...

Wetland and riparian ecosystem is an important migratory stopover for land and water birds in the East Asian-Australasian Flyway. Understanding relationship patterns between bird communities and environmental factors at multi-spatial scales within a landscape context could contribute to the conservation and management of bird biodiversity in wetlan...

Yarnvudhi A, Leksungnoen N, Siri S, Ponpithuk Y, Sukmasuang R, Duengkae P, Pongcharoen C, Sutummawong N, Marod D, Wachrinrat C, Premashthira A, Tor-ngern P, Poungcharean S, Hermuk S, Kachina P. 2022. Monetary evaluation of supporting ecosystem services as a habitat provider for birds in Thailand urban park. Biodiversitas 23: 4747-4758. Supporting s...

The Siamese fighting fish (Betta splendens) is a well-known ornamental fish and emerging model species for studying animal morphology, physiology, and behavior. A key concern of betta inbreeding is the decline in genetic diversity resulting from commercial breeding programs. Therefore, it is essential to develop markers for understanding the geneti...

Importance of the work: Forage seedlings as alternative food resources based on their nutrition are important for large prey mammals of tigers, instead of grasses. Objectives: To examine the nutritional value of predicted potential habitat area based on forage seedling species. Materials & Methods: Field data of seedlings were collected from 150 sa...

Plant diversity is important for sustainable development, particularly in watershed areas. This study explored tree population and diversity in a lower montane forest (LMF). A 16-ha permanent plot was established in LMF at Huai Kogma sub-watershed, northern Thailand. All trees with a diameter at breast height ≥ 2 cm were tagged, measured, identifie...

Bats are important reservoir hosts of emerging viruses. Recent viral outbreaks and pandemics have resulted in an increased research focus on the genetic diversity, population structure, and distribution of bat species. Lyle’s flying fox (Pteropus lylei) is widely distributed throughout central Thailand, with most colonies congregating in temples wi...

The gaur (Bos gaurus) is found throughout mainland South and Southeast Asia but is listed as an endangered species in Thailand with a decreasing population size and a reduction in suitable habitat. While gaur have shown a population recovery from 35 to 300 individuals within 30 years in the Khao Phaeng Ma (KPM) Non-Hunting Area, this has caused con...

Tigers (Panthera tigris) have disappeared from over 90% of their historical range, and extant populations face habitat loss, direct poaching, and prey depletion in otherwise suitable habitats. In Thailand, tiger numbers continue to decline due to prey depletion, yet a few strongholds remain. Recently, tigers have been detected in the Southern Weste...

Article Info Importance of the work: It is important to understand the ecological effects of gap age on the understory bird populations in the conservation of montane forest. Objectives: To study bird populations in permanent plot in Mae Sa-Kog Ma Biosphere Reserve, Northern Thailand Materials & Methods: Birds were sampled using mist-netting at 12...

The domestication of wild animals represents a major milestone for human civilization. Chicken is the largest domesticated livestock species and used for both eggs and meat. Chicken originate from the red junglefowl (Gallus gallus). Its adaptability to diverse environments and ease of selective breeding provides a unique genetic resource to address...

Centromeric satellite DNA (cen-satDNA) consists of highly divergent repeat monomers, each approximately 171 base pairs in length. Here, we investigated the genetic diversity in the centromeric region of two primate species: long-tailed (Macaca fascicularis) and rhesus (Macaca mulatta) macaques. Fluorescence in situ hybridization and bioinformatic a...

Nipah virus (NiV) is a zoonotic virus that can pose a serious threat to human and livestock health. Old-world fruit bats (Pteropus spp.) are the natural reservoir hosts for NiV, and Pteropus lylei, Lyle's flying fox, is an important host of NiV in mainland Southeast Asia. NiV can be transmitted from bats to humans directly via bat-contaminated food...

Fishes provide a unique and intriguing model system for studying the genomic origin and evolutionary mechanisms underlying sex determination and high sex-chromosome turnover. In this study, the mode of sex determination was investigated in Siamese fighting fish, a species of commercial importance. Genome-wide SNP analyses were performed on 75 indiv...

Understanding distribution patterns is essential for the long-term conservation of megafauna, particularly the Asian elephant (Elephas maximus). We investigated the dynamic occupancy of Asian elephants in the Thung Yai Naresuan West Wildlife Sanctuary in Thailand. Asian elephant occurrences were recorded during patrol activities from 2012 to 2019....

A Bayesian approach was used to develop binary quantile regression models featuring the lasso penalty. The models afford the advantages of all quantile regression models, such as robustness and detailed insights into covariate effects; they also handle issues associated with overfitting well. Thus, this model was used to investigate habitat suitabi...

The research is based on qualitative reconnaissance of the location of threat factors and Real Time Camera Traps of Thap Lan National Park during the year 2016– 2017 to analyze together with 7 physical environmental factors, 2 economic and social factors. The analysis was based on maximum entropy modeling and the model was used to create a risk lay...

The study on the spatial risk assessment of threat factors in Khao Yai National Park had the objective to detect the area of illegal forest resources offenses by the installation of a real-time camera to collect data from 2018 to 2020, totaling 3 years. The study was carried out by analyzing 8 spatial factors using a generalized linear model (GLMs)...

The African catfish (Clarias gariepinus) may exhibit the co-existence of XX/XY and ZZ/ZW sex-determination systems (SDSs). However, the SDS of African catfish might be influenced by a polygenic sex-determination (PSD) system, comprising multiple independently segregating sex “switch” loci to determine sex within a species. Here, we aimed to detect...

Duplicate control regions (CRs) have been observed in the mitochondrial genomes (mitogenomes) of most varanids. Duplicate CRs have evolved in either concerted or independent evolution in vertebrates, but whether an evolutionary pattern exists in varanids remains unknown. Therefore, we conducted this study to analyze the evolutionary patterns and ph...

Article Info AGRICULTURE AND NATURAL RESOURCES The gaur (Bos gaurus) distributes throughout mainland South and Southeast Asia. It is listed as an endangered species in Thailand, where its population has been decreasing and suitable habitat has been lost. This study explored the distribution of gaur in Thailand and assessed suitable gaur habitat in...

Chunskul J, Thongproh P, Simmasian W, Arkajag J, Tongpun S, Kanishthajata P, Prompalad S, Duangjai S, Duengkae P, Phochayavanich R, Chuaynkern C, Chuaynkern Y. 2021. Molecular identification and morphological description of Theloderma albopunctatum tadpoles from the Phu Khiao-Nam Nao Forest Complex, northeastern Thailand. Biodiversitas 22: 5145-516...

This study on the monitoring of the diversity and abundance of wildlife around the wildlife corridor area on Highway 304, which passes through Khao Yai and Thap Lan National Parks, was conducted during September 2017 and May 2021 using camera traps to constantly monitor the area. A total of at least 51 wildlife species from 30 families, 14 orders w...

Comparative chromosome maps investigating sex chromosomal linkage groups in amniotes and microsatellite repeat motifs of a male house gecko lizard (Hemidactylus frenatus, HFR) and a flat-tailed house gecko lizard (H. platyurus, HPL) of unknown sex were examined using 75 bacterial artificial chromosomes (BACs) from chicken and zebra finch genomes. N...

Article Info AGRICULTURE AND NATURAL RESOURCES The gaur (Bos gaurus) distributes throughout mainland South and Southeast Asia. It is listed as an endangered species in Thailand, where its population has been decreasing and suitable habitat has been lost. This study explored the distribution of gaur in Thailand and assessed suitable gaur habitat in...

Sex determination systems (SDSs) in anurans are diverse and have undergone independent evolutionary transitions among species. The mode of sexual reproduction of the rice field frog (Hoplobatrachus rugulosus)-an economically viable, edible amphibian species-is not well known. Previous studies have proposed that threshold temperature conditions may...

Jade perch (Scortum barcoo) is a new teleost in the developing aquaculture freshwater finfish grow-out sector in Australia and China. However, key information on the breeding sex determination system (SDS) remains poorly understood, hampering sex control programs and genetic improvement. In this study, the jade perch SDS was examined by investigati...

The snakeskin gourami (Trichopodus pectoralis) has a high meat yield and is one of the top five aquaculture freshwater fishes in Thailand. The species is not externally sexually dimorphic, and its sex determination system is unknown. Understanding the sex determination system of this species will contribute to its full-scale commercialization. In t...

Captive breeding programs are crucial to ensure the survival of endangered species and ultimately to reintroduce individuals into the wild. However, captive-bred populations can also deteriorate due to inbreeding depression and reduction of genetic variability. We genotyped a captive population of 82 individuals of the endangered Hume’s pheasant (S...

Captive breeding programs are crucial to ensure the survival of endangered species and ultimately to reintroduce individuals into the wild. However, captive-bred populations can also deteriorate due to inbreeding depression and reduction of genetic variability. We geno-typed a captive population of 82 individuals of the endangered Hume's pheasant (...