Prasun K Mukherjee

Prasun K Mukherjee
Bhabha Atomic Research Centre | BARC · Nuclear Agriculture & Bio Technology Division

Ph.D., FNASc, FNAAS, Fulbright Scholar

About

146
Publications
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Introduction
Trichoderma genetics, formulation, field trials, composting
Skills and Expertise

Publications

Publications (146)
Article
Full-text available
Eremophilanes are a large group of "sesquiterpenes" produced by plants and fungi, with more than 180 compounds being known in fungi alone. Many of these compounds are phytotoxic, antimicrobial, anticancer and immunomodulators, and hence are of great economic values. Acremeremophilanes A to O have earlier been reported in a marine isolate of Acremon...
Article
Full-text available
A single fungal metabolite induces far-reaching transcriptomic reprogramming in the plant, priming immune responses and defense, in contrast to its immunosuppressive effect on animal cells. While the negative effects of gliotoxin-producing Trichoderma strains on growth may be observed only under a particular set of laboratory conditions, gliotoxin-...
Article
Pythium aphanidermatum, an oomycete, is a serious, broad host‐range plant pathogen infecting seeds, seedlings and mature plants. It also causes serious losses under post‐harvest conditions. While isolating P. aphanidermatum from an infected bean, we have co‐isolated a fungus that was identified as Penicillium polonicum by using molecular tools. Cru...
Article
Full-text available
Trichoderma spp. are widely used as plant disease biocontrol agents in agriculture. Mycoparasitism, which is an ancestral trait of Trichoderma, is one of the most important mechanisms of reducing the pathogen inocula. Mycoparasitism is a complex physiological process that should be viewed in the broad perspective of microbial competition, and invol...
Article
Trichoderma virens produces viridin/viridiol, heptelidic (koningic) acid), several volatile sesquiterpenes and gliotoxin (Q strains) or gliovirin (P strains). We earlier reported that deletion of the terpene cyclase vir4 and a glyceraldehydes-3-phosphate dehydrogenase (GAPDH, designated as vGPD) associated with the “vir” cluster abrogated the biosy...
Article
Trichoderma virens colonizes roots and develops a symbiotic relationship with plants where the fungal partner derives nutrients from plants and offers defence, in return. Tsp1, a small secreted cysteine-rich protein, was earlier found to be upregulated in co-cultivation of T. virens with maize roots. Tsp1 is well conserved in Ascomycota division of...
Article
Translationally controlled tumour proteins (TCTPs) are omnipresent in eukaryotes and play important physiological roles. This protein is identified as an allergen in the fungi Alternaria alternata and Cladosporium herbarum, and is involved in maintaining a balance between sexual and asexual differentiation in Aspergillus nidulans. MoTCTP regulates...
Article
The anticancer antibiotic heptelidic acid is a sesquiterpene lactone produced by the beneficial plant fungus Trichoderma virens. This species has been separated into two strains, referred to as P and Q, based on its biosynthesis of secondary metabolites; notably, only P-strains were reported to produce heptelidic acid. While characterizing a Q-stra...
Article
Trichoderma virens genome harbors two isoforms of GAPDH, one (gGPD) involved in glycolysis and the other one (vGPD) in secondary metabolism. vGPD is expressed as part of the “vir” cluster responsible for the biosynthesis of volatile sesquiterpenes. The secondary metabolism-associated GAPDH is tolerant to the anti-cancer metabolite heptelidic acid (...
Article
Full-text available
Trichoderma virens is a commercial biofungicide used in agriculture. We have earlier isolated a mutant of T. virens using gamma ray-induced mutagenesis. This mutant, designated as M7, is defective in morphogenesis, secondary metabolism, and mycoparasitism. The mutant does not produce conidia, and the colony is hydrophilic. M7 cannot utilize cellulo...
Article
Small secreted cysteine-rich proteins (SSCPs) from fungi play an important role in fungi–host interactions. The plant-beneficial fungi Trichoderma spp. are in use worldwide as biocontrol agents and protect the host plant from soil-borne as well as foliar pathogens. Recently, a novel SSCP, Tsp1, has been identified in the secreted protein pool of T....
Article
Full-text available
Trichoderma virens is a plant beneficial fungus that interacts intimately with roots and boosts plant immunity against invading pathogens. Some Trichoderma species have been used to control vascular wilt pathogen Fusarium oxysporum f. sp. cubense by enhancing host plant defence. In order to understand the molecular mechanisms of such interactions o...
Chapter
Trichoderma species are filamentous ascomycetous fungi that have wide biotechnological applications in industry as well as agriculture. Having nearly 300 species, this genus represents one of the most diverse groups of fungi. Secondary metabolites are useful natural products having widespread applications in agriculture and medicine. Trichoderma sp...
Article
Full-text available
Using gamma-ray-induced mutagenesis, we have developed a mutant (named G2) of Trichoderma virens that produced two- to three-fold excesses of secondary metabolites, including viridin, viridiol, and some yet-to-be identified compounds. Consequently, this mutant had improved antibiosis against the oomycete test pathogen Pythium aphanidermatum. A tran...
Article
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Trichoderma spp. are widely used as commercial biofungicides, and most commercial formulations are conidia based. Identification of genes that regulate conidiation would thus be of help in genetic reprogramming of these species to optimize sporulation. In this study, we constructed an SSH (suppression subtractive hybridization) library from RNA sam...
Article
Full-text available
Background Trichoderma spp. are majorly composed of plant-beneficial symbionts widely used in agriculture as bio-control agents. Studying the mechanisms behind Trichoderma-derived plant benefits has yielded tangible bio-industrial products. To better take advantage of this fungal-plant symbiosis it is necessary to obtain detailed knowledge of which...
Article
Full-text available
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyses the sixth step of glycolysis, and is also known to perform other (moonlighting) activities in animal cells. We have earlier identified an additional GAPDH gene in Trichoderma virens genome. This gene is consistently associated with the vir cluster responsible for biosynthesis of a range of...
Article
Full-text available
Background Cytochrome P450s form an important group of enzymes involved in xenobiotics degradation and metabolism, both primary and secondary. These enzymes are also useful in industry as biotechnological tools for bioconversion and a few are reported to be involved in pathogenicity. Trichoderma spp. are widely used in industry and agriculture and...
Research
This study is the first time report of utilization of Trichoderma spp. isolated from different tree barks from Odisha state of India for rice crop health management and higher productivity. Six isolates of Trichoderma spp. were identified based on the morphological characteristics and species determination was performed by molecular assays. One of...
Article
This study is the first time report of utilization of Trichoderma spp. isolated from different tree barks from Odisha state of India for rice crop health management and higher productivity. Six isolates of Trichoderma spp. were identified based on the morphological characteristics and species determination was performed by molecular assays. One of...
Article
Trichoderma species are opportunistic fungi residing primarily in soil, tree bark and on wild mushrooms. Trichoderma is capable of killing other fungi and penetrating plant roots, and is commonly used as both a biofungicide and inducer of plant defence against pathogens. These fungi also exert other beneficial effects on plants including growth pro...
Chapter
Coevolution of plants with microbes has resulted in beneficial, harmful, or neutral host-parasite interactions. With the domestication of crop plants and intensive plant breeding, not only the plant genetic base narrowed down, but also it neglected the positive role of associated microbes, especially those dwelling the rhizosphere. The green revolu...
Article
Viridin group of furano-steroidal antibiotics are known to function as anti-fungal and anti-cancer agents, in addition to their roles as radio- and chemo-sensitizers. Discovered in 1945 as a metabolite of Trichoderma virens, viridins continue to receive significant attention of several synthetic chemists and clinicians as a very strong PI3 kinase i...
Article
Gliovirin is a strong anti-oomycete and a candidate anticancer compound. It is produced by “P” strains of the plant disease biocontrol fungus Trichoderma virens and is involved in biological control of certain plant pathogens. Even though the compound is known for more than three decades, neither the genes involved nor the biosynthetic pathway are...
Article
Various plasma membrane compartments in fungal cell wall are involved in a wide range of cellular functions. Eisosome protein Pil1 (phosphorylation is inhibited by long chain bases) in yeast cells are known to be involved genetic pathway that controls the cellular responses to heat stress. The PIL gene superfamily is well conserved among different...
Article
Full-text available
The cotton leafroll dwarf virus (CLRDV) is one of the most devastating pathogens of cotton. This malady, known as cotton blue disease, is widespread in South America where it causes huge crop losses. Recently the disease has been reported from India. We noticed occurrence of cotton blue disease and chickpea stunt disease in adjoining cotton and chi...
Article
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Gene cluster Peptaibiotics Secondary metabolites Trichoderma VOCs a b s t r a c t Species of the genus Trichoderma inhabit diverse environments and undergo a variety of interactions with different other organisms. Mycoparasitic Trichoderma species are successfully applied as bio-fungicides due to their plant-protecting abilities and they are prolif...
Article
The widely used biotechnologically important fungi belonging to the genus Trichoderma are rich sources of secondary metabolites. Even though the genomes of several Trichoderma spp. have been published, and data are available on the genes involved in biosynthesis of non-ribosomal peptide synthetases and polyketide synthases, no genome-wide data are...
Article
Trichoderma species are widely used in agriculture as biofungicides. These fungi are rich source of secondary metabolites and the mycoparasitic species are enriched in genes for biosynthesis of secondary metabolites. Most often, genes for secondary metabolism are clustered in fungal genomes. Previously, no systematic study was undertaken to identif...
Article
Full-text available
An endochitinase gene ‘ech42’ from the biocontrol fungus ‘Trichoderma virens’ was introduced to Brassica juncea (L). Czern and Coss via Agrobaterium tumefaciens mediated genetic transformation method. Integration and expression of the ‘ech42’ gene in transgenic lines were confirmed by PCR, RT-PCR and Southern hybridization. Transgenic lines (T1) sh...
Chapter
Trichoderma spp. are better known as suppressor of plant diseases. Trichoderma-based formulations thus dominate the biofungicide market. Intense researches however identified many traits in Trichoderma that extends the applications beyond plant protection. Various species of Trichoderma are capable of enhancing root growth and development, impartin...
Article
Gliotoxin (GT) is the most important epidithiodioxopiperazine (ETP)-type fungal toxin. Gliotoxin was originally isolated from Trichoderma species as an antibiotic substance involved in biological control of plant pathogenic fungi. A few isolates of gliotoxin-producing Trichoderma virens are commercially marketed for biological control and widely us...
Article
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Sclerotium delphinii (syn. Sclerotium rolfsii var. delphinii) is a serious pathogen infecting plants in the genus Ajuga, Arachis, Hosta, Iris, Malus and many additional herbaceous ornamental species but there has been no report of this pathogen infecting cotton. Plants infected with S. delphinii were first noticed in the fields of Wardha, India, du...
Article
The biocontrol agent, Trichoderma virens, has the ability to protect plants from pathogens by eliciting plant defense responses, involvement in mycoparasitism, or secreting antagonistic secondary metabolites. SM1, an elicitor of induced systemic resistance (ISR), was found to have three paralogs within the T. virens genome. The paralog sm2 is highl...
Article
Full-text available
Background Members of the fungal genus Trichoderma directly antagonize soil-borne fungal pathogens, and an increasing number of species are studied for their potential in biocontrol of plant pathogens in agriculture. Some species also colonize plant roots, promoting systemic resistance. The Trichoderma-root interaction is hosted by a wide range of...
Article
Full-text available
Using a gene disruption strategy, we generated mutants in the gliP locus of the plant beneficial fungus Trichoderma virens that were no longer capable of producing gliotoxin. Phenotypic assays demonstrated that the gliP-disrupted mutants grew faster, were more sensitive to oxidative stress and exhibited a sparse colony edge compared to the wild typ...
Article
Full-text available
Fungus-Fungus Interactions 1.5 million fungal species are estimated to exist on our planet which are assumed to account for at least 25% of the global biomass. Fungi inhabit a broad range of environmental niches and, due to their nutritional versatility, perform many essential processes like nutrient recycling and decomposition in global ecosystem...
Article
Full-text available
Trichoderma species are widely used in agriculture and industry as biopesticides and sources of enzymes, respectively. These fungi reproduce asexually by production of conidia and chlamydospores and in wild habitats by ascospores. Trichoderma species are efficient mycoparasites and prolific producers of secondary metabolites, some of which have cli...
Chapter
This volume brings together contributions of genomics to the study of soil fungi and plant-interacting fungi. Fungal genome projects are now far too numerous to cover in a single volume, and the species have been chosen to follow several routes through this expanding field of research: from model species for genetics like Neurospora crassa and Aspe...
Article
Full-text available
We have isolated three novel strains of Trichoderma (two T. harzianum and one T. atroviride) from wild mushroom and tree bark, and evaluated their biocontrol potential against Sclerotium delphinii infecting cultivated cotton seedlings. T. harzianum strain CICR-G, isolated as a natural mycoparasite on a tree-pathogenic Ganoderma sp. exhibited the hi...
Article
Full-text available
Trichoderma “viride” TNAU is the most widely used biofungicide in India with more than 250 registered commercial formulations being available. We have studied the phylogenetic position of this strain using a molecular marker and determined that this commercial strain is, in fact, a strain of Trichoderma asperelloides, and not T. viride. The implica...
Article
A putative terpene cyclase vir4, which is a member of a secondary metabolite cluster, has been deleted in Trichoderma virens to determine its function. The deletion mutants were compared for volatile production with the wild-type as well as two other Trichoderma spp. This gene cluster was originally predicted to function in the synthesis of viridin...
Article
Full-text available
Rice brown spot (BS) is a chronic disease that affects millions of hectares of rice every growing season, grown by some of the most resource-poor farmers. Despite its widespread occurrence and impact, much still needs to be understood about BS. Reported yield losses in relative terms vary widely from 4 to 52 %. However, accurate, systematic estimat...
Article
Full-text available
Background In fungi, environmental pH is an important signal for development, and successful host colonization depends on homeostasis. Surprisingly, little is known regarding the role of pH in fungal-fungal interactions. Species of Trichoderma grow as soil saprobes but many are primarily mycotrophic, using other fungi as hosts. Therefore, Trichoder...
Book
This volume addresses the similarities and also the differences in the genomes of soil saprophytes, symbionts, and plant pathogens by using examples of fungal species to illustrate particular principles. It analyzes how the specific interactions with the hosts and the influence of the environment may have shaped genome evolution. The relevance of f...
Chapter
This book provides an update on the advances in Trichoderma research, covering most of the aspects related to the biology, genetics, genomics and applications of Trichoderma species. An overview of the importance of Trichoderma spp. in agriculture, industry and medicine (chapter 1) is presented. The remaining articles are broadly classified under t...