Pralhad Patki

Pralhad Patki
Himalaya Drug Company · MEDICAL

MBBS, MD

About

74
Publications
27,957
Reads
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2,043
Citations
Additional affiliations
February 2002 - August 2004
SERUM INSTITUTE OF INDIA PUNE
Position
  • DEPUTY DIRECTOR , MEDICAL
February 2002 - August 2004
SERUM INSTITUTE OF INDIA PUNE
Position
  • DEPUTY DIRECTOR , MEDICAL
February 2002 - August 2004
SERUM INSTITUTE OF INDIA PUNE
Position
  • DEPUTY DIRECTOR , MEDICAL
Education
January 1978 - April 1980
NAGPUR
Field of study
  • PHARMACOLOGY

Publications

Publications (74)
Article
An indigenous herbal extract IM-133N containing extracts of Prosopis glandulosa Torr and Symplocos racemosa Roxb were evaluated for potential immunomodulatory effects using RAW264.7 and THP-1 cells. The incubation of the cells for 24 h with IM-133N over a dose range 0-125 µg/ml did not cause cytotoxicity that exceeded 10%. The results indicated tha...
Article
The present isolation and identification of napthoquinones from roots of Arnebia nobilis Reichb.f. can lead to the discovery of new anti-skin ageing ingredient in colour cosmetics. Four compounds have been isolated and purified by rigorous column chromatography. The compounds are identified as β, β-dimethylacryl alkannin (AN-I), acetoxyisovaleryl a...
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The objective of the present study is to evaluate the effect of the extract of a well-known hepatospecific polyherbal formulation, Liv.52, in an experimental model of high-fat diet (HFD)-induced nonalcoholic steatohepatitis (NASH) in rats. Feeding a HFD for 15 weeks resulted in significant impairment of the lipid profile, elevation of hepatic enzym...
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In present study two formulations of Koflet (Syrup and lozenges) were evaluated against pyridine-induced pharyngitis in rats. Topical application of 10% pyridine showed extravasation of Evans blue stain as a characteristic feature of on-going inflammation. In Addition, the levels of TNF-α (p < 0.01) and IL-6 (p < 0.01) were significantly increased...
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Bisphenol A (BPA) safety aspects on human health is debated extensively for long time. In the present study, we have studied the toxicity induced by BPA at no observed adverse effect level (NOAEL) using HepG2 cells. We report that BPA at 100 nM induced cytotoxicity to HepG2 cells as determined by MTT assay at 0-72 h. The toxicity was result of redu...
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Osteocare, a herbal formulation, has been found to be very effective in bone mineralization and support of the microstructure of bone tissue. The water-soluble components of Osteocare (WSCO) induced osteogenic activity in human osteoblast-like SaOS-2 cells. The addition of WSCO (100 μg/ml) to SaOS-2 cells was effective in increasing the cell prolif...
Article
Objective Yashada bhasma and tankana are well characterized minerals used in traditional medicine for the treatment of various skin ailments. Yashada bhasma and tankana are a unique preparation of zinc and borax respectively. The study was conducted to evaluate the in vitro inhibitory effect of yashada bhasma (YB), tankana (TA) and its combination...
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Objective. To improve the existing experimental model of croton oil-induced hemorrhoids in rats by using Evans Blue (EB) dye extravasation technique. Further, an herbal formulation (Pilex) was evaluated for its antihemorrhoidal activity in this model. Methods. Two sets of experiments were carried out: first to improve the experimental model and to...
Article
: Yashada bhasma (YB) and tankana (TA) are two natural minerals used in traditional medicine for the treatment of various skin ailments. However, the effect of these minerals on Propionibacterium acne growth and inflammation has not been studied. Objective: The in vitro inhibitory effect of YB, TA and its combination (YBTA) on P. acne growth and i...
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The objective of this study was to evaluate the synergistic activity of Bacopa monniera with Rivastigmine against aluminum-chloride (AlCl3)-induced cognitive impairment in rats. Adult male Wistar rats were divided into ten groups (n = 10) and subjected to their assigned treatments for 42 days. On the 20(th) day of the respective drug treatments, al...
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In the present study, the protective effect of Bresol(®) - a polyherbal formulation - was evaluated in an experimental model of cigarette smoke (CS)-induced COPD in rats. Ten minutes daily exposure to CS for 7 weeks caused significant elevation of TNF-α (p<0.01) and total protein (p<0.01) in the bronchoalveolar lavage fluid (BALF) of positive untre...
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HD-03/ES is a herbal formulation used for the treatment of hepatitis B. However, the molecular mechanism involved in the antihepatitis B (HBV) activity of this drug has not been studied using in vitro models. The effect of HD-03/ES on hepatitis B surface antigen (HBsAg) secretion and its gene expression was studied in transfected human hepatocarcin...
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Background This investigation was aimed to evaluate the effect of Speman®, a well known ayurvedic proprietary preparation, in an experimental model of cyclophosphamide-(CP) induced oligospermia in rats. Materials and Methods Thirty male rats were randomized in to five, equally-sized groups. Rats in group 1 served as a normal control; group 2 serve...
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Objective: The present study was aimed to evaluate the beneficial effect of Cystone® against struvite crystal growth in in vitro conditions. Methods: Various concentrations of Cystone® was prepared in 1 M magnesium acetate solution and evaluated for crystal growth inhibition assay by a well-known method called single diffusion gel growth technique...
Article
Hepatic lipid accumulation and oxidative stress contribute to non alcoholic fatty liver disease (NAFLD). Thus, we hypothesized that the hypolipidemic and antioxidant activity of quercetin would attenuate events leading to NAFLD. Addition of 2.0 mM oleic acid (OA) into the culture media induced fatty liver condition in HepG2 cells by 24h. It was mar...
Article
Aim: The aim of the study is evaluation of safety and efficacy of complete Care Herbal Toothpaste in controlling dental plaque, gingival bleeding and periodontal diseases. Materials and methods: A hundred subjects of both sex with dental plaque and other dental problems in the age group of 18-60 years, who willing gave informed written consent, wer...
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Objective: The present study was conducted to evaluate the efficacy of Abana(®) (a poly-ingredient formulation with natural constituents) on in vitro platelet aggregation and occlusion-induced deep venous thrombosis in rats. Methods: Anti-platelet property of Abana(®) was evaluated using ADP (Adenosin 5-diphosphate) and adrenaline-induced platel...
Article
HepG2 cells were rendered steatotic by supplementing 2.0mM oleic acid (OA) in the culture media for 24h. OA induced hepatic steatosis in HepG2 cells was marked by significant accumulation of lipid droplets as determined by Oil-Red-O (ORO) based colorimetric assay, increased triacylglycerol (TAG) and increased lipid peroxidation. It was also marked...
Article
Alcohol consumption has been implicated to cause severe hepatic steatosis which is mediated by alcohol dehydrogenase (ADH) activity and CYP(450) 2E1 expression. In this context, the effect of ethanol was studied for its influence on lipogenesis in HepG2 cell which is deficient of ADH and does not express CYP(450) 2E1. The results showed that ethano...
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Present study was aimed to evaluate the protective effect of Bresol®, a polyherbal formulation, on mast cell degranulation and histamine release from mast cells. Mast cell-stabilizing activity of Bresol® was evaluated against compound 48/80-induced mast cell degranulation and histamine release from rat peritoneal mast cells in ex vivo conditions. M...
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The study was carried out to standardize the oxygen radical absorption capacity (ORAC) assay for the estimation of antioxidant value of botanicals indigenous to Indian subcontinent by taking Amalaki (Emblica officinalis) and an Ayurvedic proprietary polyherbal formulation (PHF) as a representative standard. The ORAC assay for ethanolic and aqueous...
Article
Hepatocyte growth factor (HGF) plays an important role in hepatocyte proliferation. HGF expression is regulated by various signaling molecules and nuclear receptors. In the present study, LiverCare(®) (LC), a novel polyherbal formulation (The Himalaya Drug Company, Bangalore, India), was evaluated for its efficacy, using co-cultures of primary rat...
Article
Bresol-a poly-herbal formulation, has been reported to be effective against bronchial asthma and allergic rhinitis in children. In vivo studies have supported the anti-histaminic and anti-anaphylactic action of bresol. However, the mechanism of action of bresol in modulation of inflammation has not been studied at the cellular and molecular level....
Article
Justicia gendarussa Burm (Acanthaceae) is a plant used to treat inflammatory diseases such as rheumatoid arthritis. However, the mechanism involved in the anti-inflammatory properties of this plant has not been studied well. The in vitro anti-inflammatory activities of ethanol extract of Justicia gendarussa leaves (J-01) are studied here for the fi...
Article
Aim of the study: To evaluate the safety and efficacy of Ayurvedic formulation in patients with urolithiasis by assessing the symptomatic relief, reduction/expulsion of renal stones, urinary biochemical parameters. Materials and methods: In this study, 60 patients with renal calculi size between 5. mm and 12. mm were included. Thirty patients recei...
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Background: The aim of this study was to evaluate the efficacy and safety of herbal dental cream in comparison to fluoride dental cream. Objectives: Clinical evaluation of a novel herbal dental cream in plaque formation: a double-blind, randomized, controlled clinical trial. Methods: One hundred and two patients with established dental plaque...
Article
Altered copper metabolism plays a pivotal role in the onset of several hepatic disorders and glutathione (GSH) plays an important role in its homeostasis. Hepatic diseases are often implicated with decreased content of intracellular GSH. GSH depleted cells are prone to increased oxidative damage eventually leading to its death. Liv.52 is used to tr...
Article
In vivo studies have suggested the immunomodulatory properties of Septilin, an herbal preparation. This drug is being used against various types of inflammatory disorders. However, the mechanism of action of Septilin in the modulation of inflammation is not explored using suitable in vitro models. Hence, we decided to study the modulatory role of S...
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Diabetes mellitus describes a metabolic disorder of multiple etiologies characterized by insulin resistance, relative insulin deficiency and hyperglycemia with disturbances of carbohydrate, fat and protein metabolism. The goal for treatment of diabetes is to prevent its acute manifestations and long-term microvascular and macrovascular complication...
Article
Medical management of urolithiasis is still a challenge for modern medical practice. In the present study, Cystone tablet, an Ayurvedic formulation claimed to be useful in urolithiasis was evaluated for its safety and efficacy in reduction or expulsion of ureteric calculi and to assess the role of Cystone in relieving the clinical symptoms. Approac...
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Gentamicin is a widely used antibiotic for the treatment of adverse urinary tract infections (UTI), which in turn causes nephrotoxicity to uroepithelial cells and hence an alternative safe herbal remedy is much desired to compensate these toxic effects. The bacterial adhesion to the uroepithelial cells is the primary step in UTI and it induces vari...
Article
The present study was designed to study the genotypes associated with different groups of chronic liver disease and to see their response to HD-03/ES (an antiviral herbal molecule) on chronic HBV patients. A total of 51 patients of chronic liver disease were recruited in the study and were given HD-03/ES, two capsules twice daily for 6 months. Live...
Article
The liver is a target of injuries by a number of hepatotoxins such as viruses, bacteria, chemicals, medicines and alcohol. If not treated early such injuries can lead to chronic liver disorders. A number of herbal formulations have been claimed to be effective in managing liver disorders. In the present study a herbal formulation has been clinicall...
Article
Hepatitis B virus (HBV) infection is highly prevalent world over, especially in developing countries. A new recombinant hepatitis B virus (GeneVac-B; Serum Institute of India Ltd.) vaccine is developed using Hansenula polymorpha yeast. We decided to assess the immunogenicity, and reactogenicity of this vaccine in a large adult population. Seven hun...
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Objective: To study, the anticataract activity of lisinopril and enalapril on cataract induced by glucose, in goat lenses. Methods: Goat lenses were incubated in artificial aqueous humor containing 55 mM glucose (cataractogenesis) with lisinopril or enalapril in different concentrations at room temperature for 72 h. Biochemical parameters studied i...
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Objectives: To study, the anticataract activity of lisinopril and enalapril on cataract induced by glucose, in goat lenses. Materials and Methods: Goat lenses were incubated in artificial aqueous humor containing 55 mM glucose (cataractogenesis) with lisinopril or enalapril in different concentrations at room temperature for 72 h. Biochemical param...
Article
The immunoadjuvant potential of Asparagus racemosus (Willd.) Family (Liliaceae) aqueous root extract was evaluated in experimental animals immunized with diphtheria, tetanus, pertussis (DTP) vaccine. Immunostimulation was evaluated using serological and hematological parameters. Oral administration of test material at 100 mg/kg per day dose for 15...
Article
The results of the clinical assessment of a multiherbal indigenous formulation on 22 patients with bleeding piles are reported.
Article
The immunomodulatory activities of an Indian Ayurvedic medicinal preparation, i.e. extracts from Ashwagandha, Withania somnifera (L.) Dunal (Solanaceae), namely WST and WS2, were studied in mice for immune inflammation: active paw anaphylaxis and delayed type hypersensitivity (DTH). Immunomodulatory effect was assessed in If IgE-mediated anaphylaxi...
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In1975theWorldHealth Assembly requested theDirector-General toadvise MemberStates onthe selection andprocurement ofessential drugs corresponding totheir national health needs. Wereport here theresults ofastudy oftheprescribing patterns andrational drugutilization ofmedical practitioners ofPune, anindustrial city inthewestofIndia, whichwasundertaken...
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Full-text available
In 1975 the World Health Assembly requested the Director-General to advise Member States on the selection and procurement of essential drugs corresponding to their national health needs. We report here the results of a study of the prescribing patterns and rational drug utilization of medical practitioners of Pune, an industrial city in the west of...
Article
Aqueous extract, alcohol extract, acetone extract and petroleum ether extract suspensions of stem of Tinospora cordifolia for some of the pharmacological activities namely the antistress activity by the mice swimming endurance test, anabolic activity by noting gain in body weights and effect on cyclophosphamide induced immunosuppression in mice. A...
Article
The immunomodulatory activity of an Indian Ayurvedic medicinal preparation, Ashwagandna (Withania somnifera (L. Dunal)) was studied in mice with myelosuppression induced by one or more of the following three compounds: cyclophosphamide, azathioprin, or prednisolone. The assessment of immunomodulatory activity was carried out by hematological and se...
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A herbal hepatoprotective formulation Liv 52 down regulated the tumour necrosis factor (TNF) production in Charles Foster Rats treated with CCl4. Inhibition of TNF activity was proportional to the hepatoprotective activity.
Article
Administrations of hepatotoxicants namely carbon tetrachloride (CCl4:0.4 ml in 1.2 ml of liquid paraffin) and ANIT (1 ml of 1.5% solution in liquid paraffin) in Charles foster rats (force fed) and D-galactosamine (8 mg in water per swiss albino mouse, ip) induce the release of TNF-alpha in case of CCl4 and D-galactosamine. High TNF-alpha level was...
Article
The clinical efficacy of a herbomineral formulation containing roots of Withania somnifera, the stem of Boswellia serrata, rhizomes of Curcuma longa and a zinc complex (Articulin-F), was evaluated in a randomized, double-blind, placebo controlled, cross-over study in patients with osteoarthritis. After a one-month single blind run-in period, 42 pat...
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To evaluate the antihypertensive activity of potassium given alone or in combination with magnesium in patients with mild hypertension. A double blind, randomised, placebo controlled, crossover trial of 32 weeks' duration. Cardiology outpatient department, Sassoon General Hospitals, Pune, India. 37 Adults with mild hypertension (diastolic blood pre...
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Terminalia belerica (behada) fruit has been evaluated as a promising agent against E. histolytica and a variety of bacteria associated with dysentery and diarrhoea. These in vitro studies were extended further for evaluating clinical efficacy among patients of acute and chronic cases of diarrhoea and dysentery. The Studies were carried out on 25 pa...
Article
Seventy obese subjects were randomised into four groups. Ayurvedic drug treatments were given for three months while one group received a placebo. Physical, clinical and pathological investigations were carried out at regular intervals. A significant weight loss was observed in drug therapy groups when compared with the placebo. Body measurements s...
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In this well documented write-up, the use of various male sex remedies like Tentex forte, Himcolin, Speman and Speman forte has been reviewed in male sex disorders. Their successful employment in conditions like impotence, male infertility, premature ejaculation, nocturnal emissions etc has been emphasised. INTRODUCTION "East is east and West is we...
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'Arogyavardhini'-an indigenous formulation was evaluated for its hepatoprotective activity in rats, using two models of carbon tetrachloride (CCl4) hepatic damage, one simulating vital hepatitis and the other simulating fatty change. The protective effect was assessed from serum aspartate transaminase (AST) and alkaline phosphatase levels and from...