Prajakta Deshpande

Prajakta Deshpande
University of Dayton | UD · Department of Biology

Master of Science

About

19
Publications
2,172
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
132
Citations
Citations since 2016
18 Research Items
124 Citations
2016201720182019202020212022010203040
2016201720182019202020212022010203040
2016201720182019202020212022010203040
2016201720182019202020212022010203040

Publications

Publications (19)
Chapter
RNA is an important connecting link between DNA and proteins. Levels of RNA within a cell or a tissue serve as the unique genetic signatures, which can help in correlating gene expression to the resultant phenotype(s) during development and disease. Transcriptomics is the study of all RNAs expressed/available in cells or tissues that allow study of...
Article
Full-text available
Cell death maintains tissue homeostasis by eliminating dispensable cells. Misregulation of cell death is seen in diseases like cancer, neurodegeneration, etc. Therefore, cell death assays like TUNEL have become reliable tools, where fragmented DNA of dying cells gets fluorescently labeled and can be detected under microscope. We used TUNEL assay in...
Chapter
A hallmark of development, aging, represents the changes in the body over a period of time. At cellular level, the basic unit of organization in multicellular organism, aging is associated with deterioration of cellular function(s) and replicative ability. These changes include accumulation of genetic damage, shortening of telomere length, metaboli...
Article
Alzheimer’s disease (AD) is a debilitating neurodegenerative disorder with no cure and few treatments. AD causes profound cognitive deficits and memory impairments. Accumulation of the peptide amyloid beta plaque is one of the characteristics of the disease. To understand the progression of Alzheimer’s disease, studies often rely on ectopic express...
Article
Background: Alzheimer's disease (AD), an age-related progressive neurodegenerative disorder, exhibits reduced cognitive functions with no cure to date. One of the reasons for AD is the extracellular accumulation of Amyloid-beta 42 (Aβ42) plaques. Misexpression of human Aβ42 in the developing retina of Drosophila exhibits AD-like neuropathology. Ac...
Article
Full-text available
Newts utilize their unique genes to restore missing parts by strategic regulation of conserved signaling pathways. Lack of genetic tools pose challenges to determine the function of such genes. Therefore, we used the Drosophila eye model to demonstrate the potential of 5 unique newt (Notophthalmus viridescens) gene(s), viropana1-viropana5 (vna1-vna...
Article
Full-text available
Numerous imaging modules are utilized to study changes that occur during cellular processes. Besides qualitative (immunohistochemical) or semiquantitative (Western blot) approaches, direct quantitation method(s) for detecting and analyzing signal intensities for disease(s) biomarkers are lacking. Thus, there is a need to develop method(s) to quanti...
Preprint
Full-text available
A fundamental process of regeneration, which varies among animals, recruits conserved signaling pathways to restore missing parts. Only a few animals like newts can repeatedly regenerate lost body parts throughout their lifespan that can be attributed to strategic regulation of conserved signaling pathways by newt's regeneration tool-kit genes. Her...
Article
Micro abstract The study focuses on the RASSF1A gene promoter methylation and its impact on the clinicopathological features in Indian breast cancer patients highlighting the differences from other genetically different population. We found that RASFF1A gene methylation has significant impact on tumor size and tumor grade. The work carries high sig...
Article
Full-text available
To understand the progression of Alzheimer's disease, studies often rely on ectopic expression of amyloid-beta 42 (Aβ42) throughout an entire tissue. Uniform ectopic expression of Aβ42 may obscure cell-cell interactions that contribute to the progression of the disease. We developed a two-clone system to study the signaling cross talk between GFP-l...
Article
Full-text available
During development, regulation of organ size requires a balance between cell proliferation, growth and cell death. Dysregulation of these fundamental processes can cause a variety of diseases. Excessive cell proliferation results in cancer whereas excessive cell death results in neurodegenerative disorders. Many signaling pathways known-to-date hav...
Article
During organogenesis, cell proliferation is followed by the differentiation of specific cell types to form an organ. Any aberration in differentiation can result in developmental defects, which can result in a partial to a near-complete loss of an organ. We employ the Drosophila eye model to understand the genetic and molecular mechanisms involved...
Article
Full-text available
Alzheimer's disease (AD, OMIM: 104300) is an age-related disorder that affects millions of people. One of the underlying causes of AD is generation of hydrophobic amyloid-beta 42 (Aβ42) peptides that accumulate to form amyloid plaques. These plaques induce oxidative stress and aberrant signaling, which result in the death of neurons and other patho...
Article
Full-text available
Amyotrophic Lateral Sclerosis (ALS), a late-onset neurodegenerative disorder characterized by the loss of motor neurons in the central nervous system, has no known cure to-date. Disease causing mutations in human Fused in Sarcoma (FUS) leads to aggressive and juvenile onset of ALS. FUS is a well-conserved protein across different species, which pla...
Article
Full-text available
Alzheimer’s disease (hereafter AD) is a progressive neurodegenerative disorder that affects the central nervous system. There are multiple factors that cause AD, viz., accumulation of extracellular Amyloid-beta 42 plaques, intracellular hyper-phosphorylated Tau tangles, generation of reactive oxygen species due to mitochondrial dysfunction and gene...
Article
Backgrond: Anemia and poor nutrition status, especially in pregnant women, are partially attributable to helminthes infections. The majority of the global burden is considered due to the five main parasites: Ascaris lumbricoides, Necator americanus, Ancylostoma dudonale, Trichuris trichiura and Giardia duodenalis, Entamoeba histolytica. Diagnosis o...
Article
Breast cancer is the most common form of malignant disease in women worldwide and is the major cause of mortality among women. It is a heterogeneous disease with histopathological, genetic and epigenetic characteristics. Epigenetic alterations such as in DNA methylation, characterized by addition of a methyl group at the carbon-5 position of cytosi...
Article
Full-text available
Transposable elements (TEs) comprise almost 45% of the human genome. There are DNA transposons and RNA transposons. Retrotransposons like long interspersed nuclear element 1 (LINE 1) is abundant among all the TEs and present in 17% of the genome. These elements have repetitive sequences. Retrotransposons are regulated by epigenetic modification lik...

Questions

Question (1)
Question
I did protein extraction from 75 eye imaginal discs and lysed with 40ul RIPA buffer and 1ul of PMSF and centrifuged at 13000 rpm for 20min. I took the supernatant and quantified by using Nanodrop. When I used RIPA buffer as a Blank, the concentration of protein was 0.2mg/ml but when I used water as a Blank, the concentration of protein was 23mg/ml. What do you recommend?

Network

Cited By