
Pooja GhoshJIS Institute of Advanced Studies & Research (JISIASR) Kolkata · Centre for Interdisciplinary Sciences
Pooja Ghosh
Ph.D
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34
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Introduction
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December 2012 - December 2014
Publications
Publications (34)
The idea of designing novel anti-amyloid therapeutic agents has generated a lot of scientific interest and the potential for treating a variety of human pathophysiologies, such as neurodegenerative and non-neuropathic diseases linked to amyloid protein aggregation. To address this, different small molecules, peptides, surfactants, nanomaterials, et...
Lipoic acid (LA) and dihydrolipoic acid (DHLA) are well established antioxidants to scavenge reactive oxygen species (ROS). However, they are carboxylates with ca. 4.7 pKa making them negatively charged at physiological pH (7.4) reducing their passive diffusion through cell membranes. LA is known to be capable of reducing protein fibrillation. Inco...
To address the obstacles in insulin protein homeostasis leading to the formation of neurotoxic amyloid plaques associated with different diseases, herein we have synthesized block copolymers using the reversible addition-fragmentation chain transfer (RAFT) polymerization method, composed of tert-butoxycarbonyl (Boc) protected leucine and acetyl (Ac...
Advanced glycation end products (AGEs) formed through nonenzymatic glycation results in diseases including diabetic complications, Alzheimer’s disease and atherosclerosis etc. The development of novel antiglycating agents has thus become an important area of research. In our present work, we have reported the inhibitory activity of morin loaded PLG...
Endometriosis is a debilitating gynecological disorder in women of reproductive age. Laparoscopy, a minimally invasive surgical procedure, provides a definitive diagnosis of the disease. Current treatments, including hormonal therapy and pain medication, are often associated with undesirable side effects limiting their long-term usage. This calls f...
A family of amphiphilic diblock copolymers containing a hydrophobic polyisobutylene (PIB, Mn = 1000 g mol-1) segment and a hydrophilic block with sugar pendants has been synthesized by combining living cationic and reversible addition-fragmentation chain transfer (RAFT) polymerization techniques; to explore their potential in insulin fibrillation i...
The rapid growth in the applications of nanoparticles (NPs) in biomedical and pharmaceutical fields requires an understanding of the interactions with the lipid bilayer membrane for further in vivo studies. Zwitterionic 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), negatively charged 1,2-dioleoyl-sn-glycero-3-phospho-l-serine (DOPS) and positive...
To develop anti-amyloidogenic inhibitors for ameliorating the treatment of diabetes, herein, we have synthesised amphiphilic block copolymers with side-chain fatty acid (FA) moieties via reversible addition fragmentation chain-transfer (RAFT) polymerization. We addressed the unexplored role of FA pendants in the FA-tethered block copolymers (FABC)...
Amyloids are highly ordered aggregates of misfolded proteins, related to a large number of neurodegenerative diseases including Alzheimer’s, Parkinson’s, type II diabetes and many other debilitating disorders. The misfolded proteins are most commonly [Formula: see text]-sheet rich fibrillar aggregates, and one of the most common therapeutic approac...
Nanoencapsulation has emerged as a promising approach for the effective delivery of poorly aqueous soluble compounds. The current study focuses on the preparation of human serum albumin (HSA)-based nanoparticles (NPs) and poly lactic-co-glycolic acid (PLGA)-based nanoparticles for effective delivery of the morin-Cu(II) complex. The NPs were analyze...
Macromolecular engineering presents a pleasant tactic for planning the construction of desired molecular architectures from nano to macro configurations. The mechanistic pathways and synthetic features of macromolecular designs mostly rely on the precursor composition, topology, functionality and complexity in architectures. Specifically, macromole...
The design and development of side‐chain amino‐acid‐based polymeric nanostructures have attracted a significant research interest as they bring a remarkable revolution in various territories of the biomedical field. The incorporation of amino acid moieties into the side chain of synthetic polymeric scaffolds exhibits several beneficial properties l...
The concept of developing novel anti-amyloid inhibitors in the scientific community has engrossed remarkable research interests and embraced significant potential to resolve numerous pathological conditions including neurological as well as non-neuropathic disorders associated with amyloid protein aggregation. These pathological conditions have har...
In recent decades, polymeric materials have been
extensively investigated for their widespread therapeutic applications
in various biomedical fields. In particular, biomolecule conjugated
polymers continue to draw attention due to their distinctive
physicochemical and biological properties. By appending biomolecules
on polymeric constructs, these s...
Nowadays, one of the major challenges in biomedical and biopharmaceutical field is designing novel and effective anti-amyloidogenic inhibitors for the treatment of various human pathophysiologies associated with protein aggregation. In this milieu, numerous small molecules, polyphenols, surfactants, nanoparticles, etc. have been extensively studied...
Protein misfolding and aggregation have attracted immense research interests due to their connection with an array of degenerative diseases including neurodegenerative disorders and non-neuropathic diseases. Inhibition or diminution of the formation of protein aggregates is considered as a potential therapeutic strategy to cure these debilitating m...
Formation of insulin amyloid fibrils is considered as the hallmarks of several debilitating pathological conditions. To develop effective therapeutics that are able to control the amyloidogenesis process and inhibit fibril formation, herein we have synthesized side-chain proline (Pro)-based homopolymer and block copolymers via reversible addition-f...
Curcumin, a naturally occurring polyphenol despite having therapeutic properties, has limited application due to its poor solubility in aqueous medium. The aim of this study is to explore a new protein source developed as a protein film, for the delivery of curcumin. The cataractous eye protein isolate (CEPI) is a mixture of proteins obtained after...
Flavonoids like quercetin and myricetin serve as naturally occurring antioxidants but their bioactivity is limited due to low aqueous solubility and oxidation under physiological conditions. In this current study, the antioxidant activity of quercetin and myricetin loaded chitosan nanoparticles during the induced oxidation of Ribonuclease A (RNase...
The interactions of naringenin (NG) and naringin (NR) with Hen Egg White Lysozyme (HEWL) in aqueous medium have been investigated using UV-vis spectroscopy, steady-state fluorescence, circular dichroism (CD), Fourier Transform infrared spectroscopy (FT-IR) and molecular docking analyses. Both NG and NR can quench the intrinsic fluorescence of HEWL...
Flavonoids are biologically imperative compounds used as anti-oxidants, anti-cancer, anti-bacterial agents etc. The current work reports comprehensive binding studies of two important flavonoids, 6-hydroxyflavone and 5,7-dihydroxyflavone (chrysin) with bovine hemoglobin (BHb) at 298K and 308K, in aqueous medium using UV-vis spectroscopy, steady sta...
Understanding the interaction of proteins with nanoparticles has become an important area of research in biomedical and pharmaceutical fields. Morin is a flavonol which shows several properties including antioxidant, anticancer and anti-inflammatory activities. However the major limitation is its poor aqueous solubility. Therefore, morin loaded pol...
Glycation of ocular lens proteins plays a vital role in the development of diabetic cataract. In order to investigate the role of glycation in cataractogenesis, the extent of glycation of human γB-crystallin was determined by an in vitro glycation study in a solution of high glucose content for upto 28 days. The glycated protein has been purified a...
The polyphenols morin and epicatechin exhibit properties that include antioxidant, anticancer, and anti-inflammatory activities, etc. However, their clinical application is hampered by their poor aqueous solubility and bioavailability. In the present study an attempt has been made to overcome these drawbacks by encapsulating morin and epicatechin s...
Reducing sugars can modify protein structures in vivo via non-enzymatic methods were identified in different cases of diabetes and glycemia. The synthesis and characterization of glycated analogue of human serum albumin (HSA) has been reported recently by our group (Singha Roy et al. in J Biomol Struct Dyn 2015. doi:10. 1080/ 07391102. 2015. 109474...
The dietary components, flavonoids, are important for their anti-oxidant properties and the ability to act as metal ion chelators. The characterization of the morin-Cu(ii) complex is executed using elemental analysis, FTIR and mass spectroscopy. DNA cleaving and cell cytotoxicity properties followed by serum albumin binding have been investigated i...
The interaction between hesperetin and hen egg lysozyme has been investigated using UV-vis, steady-state fluorescence, synchronous fluorescence, three dimensional (3D) fluorescence, circular dichroism (CD) and molecular docking studies. The structural characteristics of hesperetin are monitored at different pH conditions (5.4, 6.4, 7.0, 7.4, 8.0, 8...
Fisetin is a well known flavonoid that shows several properties such as antioxidant, antiviral and anticancer activities. Its use in the pharmaceutical field is limited due to its poor aqueous solubility which results in poor bioavailability and poor permeability. The aim of our present study is to prepare fisetin loaded human serum albumin nanopar...
Diabetes is a major problem in the world. The proteins became modified during glycation after reacting with the reducing sugars (e.g. D-glucose) via non-enzymatic pathways. The glycated analogue of human serum albumin (HSA) has been characterized with the help of multi-spectroscopic methods. It has been observed that six glucose molecules can bind...
The bark extract of Mimusops elengi is rich in different types of plant secondary metabolites such as flavonoids, tannins, triterpenoids and saponins. The present study shows the usefulness of the bark extract of Mimusops elengi for the green synthesis of gold nanoparticles in water at room temperature under very mild conditions. The synthesis of t...