Po-Yu Chu

• Chang Gung University

The ability to predict or detect colorectal cancer (CRC) recurrence early after surgery enables physicians to apply appropriate treatment plans and different follow-up strategies to improve patient survival. Overall, 30–50% of CRC patients experience cancer recurrence after radical surgery, but current surveillance tools have limitations in the pre...

Optically induced dielectrophoresis (ODEP)-based microparticle sorting and separation is regarded as promising. However, current methods normally lack the downstream process for the transportation and collection of separated microparticles, which could limit its applications. To address this issue, an ODEP microfluidic chip encompassing three micro...

The analysis of circulating tumor cells (CTCs) at the molecular level holds great promise for several clinical applications. For this goal, the harvest of high-purity, size-sorted CTCs with different subtypes from a blood sample are important. For this purpose, a two-step CTC isolation protocol was proposed, by which the immunomagnetic beads-based...

Analysis of circulating tumor cells (CTCs) holds promise to diagnose cancer or monitor its development. Among the methods, counting CTC numbers in blood samples could be the simplest way to implement it. Nevertheless, its clinical utility has not yet been fully accepted. The reasons could be due to the rarity and heterogeneity of CTCs in blood samp...

For the rapid detection of bacteria in a blood sample, nucleic acid amplification-based assays are believed to be promising. Nevertheless, the nucleic acids released from the dead blood cells or bacteria could affect the assay performance. This highlights the importance of the isolation of live bacteria from blood samples. To address this issue, th...

In biomedical diagnosis, the efficient separation and purification of specific targets from clinical samples is the desired first step. Herein, the concept of virtual filter membranes based on optically-induced dielectrophoresis (ODEP) manipulation in a microfluidic channel is proposed as a light screening membrane for the separation of polystyrene...

Magnetic beads improve biosensing performance by means of their small volume and controllability by magnetic force. In this study, a new technique composed of optically induced dielectrodphoresis (ODEP) manipulation and image processing was used to enhance the signal-to-noise ratio of the fluorescence for stained magnetic beads. According to natura...

Optically induced dielectrophoresis (ODEP) is effective for cell manipulation. However, its utilization has been limited by the requirement of solution with low conductivity. This issue has been ignored in ODEP-relevant studies. To address this issue, this study aims to investigate to what extent the cell viability and performance of ODEP-based cel...

Conventional circulating tumor cell (CTC) enumeration could ignore the CTCs more relevant to cancer metastasis. Thus, negative selection CTC enumeration was proposed, by which information on two cellular biomarkers (numbers of CTCs and CD45neg EpCAMneg cells) can be obtained. By combining this approach with the conventional biomarker carcinoembryon...

To develop a lab on a chip (LOC) integrated with both sensor and actuator functions, a novel two-in-one system based on optical-driven manipulation and sensing in a microfluidics setup based on a hydrogenated amorphous silicon (a-Si:H) layer on an indium tin oxide/glass is first realized. A high-intensity discharge xenon lamp functioned as the ligh...

Negative selection-based circulating tumor cell (CTC) isolation is able to harvest viable, label-free, and clinically meaningful CTCs from the cancer patients’ blood. Nevertheless, its main shortcoming is its inability to isolate high-purity CTCs, restricting subsequent CTC-related analysis. To address this issue, this study proposed a two-step opt...

Cell manipulation using optically induced dielectrophoresis (ODEP) in microfluidic systems has attracted the interest of scientists due to its simplicity. Although this technique has been successfully demonstrated for various applications, one fundamental issue has to be addressed—Whether, the ODEP field affects the native properties of cells. To a...

Antibiotics resistance is regarded as a potential threat of bacterial infection, making certain bacteria survive and grow during therapy and thus resulting in therapeutic failure. To achieve a rapid screen of antibiotic-resistant bacteria, it is urgent to develop a bacteria culture-independent screening method. Integrating with properties of optica...

Different levels of antibiotic resistance reside in a bacterial clone (heteroresistance) is regarded as an important cause leading to development of superbugs and treatment failure of infectious diseases. However, phenotypical detection of bacterial heteroresistance is not available in current clinical practice or by present analytical tools. To ad...

Chemotherapy resistance is regarded as the main cause of therapeutic failure, making certain cancer cells survival after chemotherapy. To investigate the mechanism underlying chemotherapy resistance, an effective cell sorting technique is urgent to be developed for isolating the cancer cells with different response to anti-cancer drugs. Integrating...

Magnetic microbeads are widely utilized in microfluidic systems for various applications. For these tasks, the effective and efficient manipulation of magnetic microbeads is important. Among the techniques for microparticle manipulation, the optically induced electrokinetic (OEK) [e.g., optically induced dielectrophoresis (ODEP) or light-actuated A...

The integration of optically induced dielectrophoresis (ODEP)-based cell manipulation in a microfluidic system has been actively proposed for various biomedical researches. To realize ODEP-based cell manipulation for a special application, the selection of an appropriate ODEP operating condition is critical. The magnitude and frequency of the exert...

The heterogeneity of the drug resistance of cancer cells in a tumor is regarded as an important cause of therapeutic resistance and thus treatment failure. An understanding of the heterogeneity of cancer cells in a tumor in terms of their anti-cancer drug resistance is of great value for clinical applications or fundamental studies. To achieve this...