About
68
Publications
13,560
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
1,055
Citations
Introduction
I am interested in studying fish immunology and host-pathogen interaction (focus on toll-like receptors) to develop better prophylactic measures for aquatic animals.
I make collaborations with researchers all over the world, such as Malaysia, Thailand, Viet Nam, Israel, the U.S., the U.K....etc.
I am a reviewer for several journals, such as Fish & Shellfish Immunology, PLOS ONE, Scientific Reports, PeerJ and Journal of Fish Diseases...etc.
http://scholars.ntou.edu.tw/cris/rp/rp00398
Current institution
Additional affiliations
July 2015 - June 2016
Position
- PostDoc Position
Description
- Study interaction between CyHV-3 and common carp.
October 2011 - May 2015
September 2014 - present
Position
- Demonstrator
Description
- Biological Enhanced Skills Training (BEST) : Help students to be familiar with Minitab software. Organismal Biology: Help students in laboratory based practical sessions.
Publications
Publications (68)
Nile tilapia (Oreochromis niloticus) is one of the most important food fish in the world. However, the farming industry has encountered significant challenges, such as pathogen infections. Toll-like receptors (TLRs) play an essential role in the initiation of the innate immune system against pathogens. Sterile alpha and TIR motif-containing protein...
Nile tilapia (Oreochromis niloticus) is one of the major food fish worldwide. The farming business, on the other hand, has faced considerable obstacles, such as disease infestations. Toll-like receptors (TLRs) play an important function in the activation of the innate immune system in response to infections. Unc-93 homolog B1 (UNC93B1) is a key reg...
The aquaculture sector has recognized antimicrobial resistance (AMR) as a significant challenge in recent years. The increase in antibiotic resistance among farmed fish is a notable concern for the aquaculture industry. Due to the frequent bacterial infections in fish populations, antibiotics must be regularly administered, resulting in their ongoi...
This study provides a comprehensive summary of the findings regarding the application and diagnostic efficacy of droplet digital PCR (ddPCR) in detecting viral and bacterial pathogens in aquaculture. Utilizing a systematic search of four databases up to 6 November 2023, we identified studies where ddPCR was deployed for pathogen detection in aquacu...
This study aims to develop an alternative aquafeed derived from insect meal for Lates calcarifer juveniles, with the objectives of exploring the physiological performance, biological parameters, and economic analysis of substituting fishmeal (FM) with defatted black soldier fly (Hermetia illucens) larvae (BSFL) as part of the diet of L. calcarifer...
Snakehead (Channa lucius Cuvier, 1831) is a species of potential aquaculture interest in the Mekong Delta, Vietnam. However, their optimum environmental conditions have yet to be determined. This study aims to study the hatching success and larval and fry growth of C. lucius at six pH levels (5.5, 6.0, 6.5, 7.0, 7.5, and 8.0). Two consecutive exper...
Among the minority seawater species in the ornamental fish market, clownfish (Amphiprion ocellaris) have a high market value due to their vibrant appearance. Astaxanthin, a promising coloring agent, is applied in diets as a food additive to enhance the physical appearance of clownfish. However, as the retail price of astaxanthin has been inflating,...
To encourage aquaculture sustainability and lower fish production costs, fish products need to be substituted with sustainable ingredients in aquafeeds. Toward this goal, we examined whether a natural mix of highly concentrated free amino acids could alleviate the negative effects of low-fish meal (FM) diets on Nile tilapia in terms of growth perfo...
Rachycentron canadum (cobia) is a marine fish species of high economic value in aquaculture due to its fast growth rate and good feed conversion efficacy. Regrettably, the industry has been affected by significant setbacks from high mortality due to diseases. Consequently, an improved perception of innate immunity correlated to each mucosal-associa...
In this study dietary Scutellaria baicalensis extract (SBE) was used to improve the shrimps' immune response and its resistance to Vibrio parahaemolyticus. SBE obtained by solid-liquid extraction (SLE) has shown stronger antibacterial activity against V. parahaemolyticus compared to extracts obtained through the pressurized liquid extraction (PLE)...
This study aims to identify a local red microalga in Taiwan through morphology and molecular phylogeny. Then, to determine the optimal culture conditions, as in temperature, light intensity and light quality, for the mass production of B-phycoerythrin using the microalga, growth and pigment content of the microalga under different cultivation condi...
White shrimp (Penaeus vannamei) is an important culture species in Taiwan but often encounters disease infection by Vibrio parahaemolyticus that cause acute hepatopancreatic necrosis disease (AHPND). This study investigates the effects of dietary supplementation of Leuconostoc mesenteroide B4 and its fermentate (dextran) on the immune response, int...
Zalpha (Z␣) domains bind to left-handed Z-DNA and Z-RNA. The Z␣ domain protein family includes cellular (ADAR1, ZBP1 and PKZ) and viral (vaccinia virus E3 and cyprinid herpesvirus 3 (CyHV-3) ORF112) proteins. We studied CyHV-3 ORF112, which contains an intrinsically disordered region and a Z␣ domain. Genome editing of CyHV-3 indicated that the expr...
Bioactive compounds extracted from natural renewable sources have attracted an increased interest from both industry and academia. Recently, algae have been highlighted as promising sources of bioactive compounds, such as polyphenols, polysaccharides, fatty acids, proteins, and pigments, which can be used as functional ingredients in many industria...
Nile tilapia (Oreochromis niloticus) is one of the most important food fish in the world. However, the farming industry has encountered significant challenges, such as pathogen infections. Toll-like receptors (TLRs) play an essential role in the initiation of the innate immune system against pathogens. Sterile alpha and TIR motif-containing protein...
The cryptophyte alga Proteomonas sulcata is rich in unsaturated fatty acids, phycoerythrin, and other biologically active substances and is suitable for aquatic feed, biomedical applications, and other related industries. To stimulate and increase the production of P. sulcata, we investigated the optimal culture conditions and two-stage culture of...
Aquaculture plays a key role in food production globally and provides a valuable source of protein and nutrition, addressing a worldwide growing demand. Oplegnathus punctatus (spotted knifejaw) is an economically important fish species with a high market value and demand. Previous studies on O. punctatus focused mainly on gonadal development, chrom...
Nile tilapia (Oreochromis niloticus) is an important food fish species that is mainly cultivated in tropical and subtropical countries. However, microbial diseases have created various difficulties for this industry. The fundamental prerequisite for tackling disease outbreak prevention and disease resistance is to know how hosts’ immune responses a...
An eight-week study was conducted to investigate the effects of dietary free amino acids (KFAAs; Kera-Stim®50) on the growth performance, biochemical parameters, innate immune responses, muscle composition, digestive enzyme activity, and muscle fatty acid composition of Nile tilapia (Oreochromis niloticus). Five diets were supplemented with KFAAs a...
Trivalent arsenic (As (III)) contamination in the marine environment can produce adverse effects in crustaceans. The present study investigated the chronic toxicity of As (III) in white shrimp (Penaeus vannamei) by analyzing the tissue bioaccumulation and non-specific immune responses. Shrimps were exposed to 0 (control), 50, 500, and 2500 μg/L of...
Cobia (Rachycentron canadum) is a marine fish of high economic value that grows at a fast rate. However, intensive fish farming has led to disease outbreaks in cobia cultures, which is highly costly to the industry. The impact of infectious diseases on cobia production has led to the inappropriate and increased use of chemicals and antibiotics, whi...
This study evaluated the effects of the feeding of spent mushroom substrate from Agaricus blazei on Nile tilapia (Oreochromis niloticus). The safety of 0–1000 μg/mL A. blazei spent substrate water extract (ABSSE) was demonstrated in the primary hepatic and splenic macrophages and the THK cell line (a cell line with characteristics of melanomacropha...
Mercury (Hg) is a dangerous heavy metal that can accumulate in fish and is harmful when consumed by humans. This study investigated the bioaccumulation of mercury in the form of methylmercury (MeHg) and evaluated nonspecific immune responses such as phagocytic activity and superoxide anion (O2−) production in hybrid grouper (Epinephelus fuscoguttat...
An experiment was conducted to investigate the effects of Aegle marmelos fruit (AMF) extract on the growth performance, biochemical parameters, immune response, antioxidative capacity, and digestive enzyme activity of Nile tilapia (Oreochromis niloticus). Fish were fed a diet supplemented with AMF at concentrations of 0 (AMF0; control), 5 (AMF5), 1...
The gastrointestinal immune system plays an important role in immune homeostasis regulation. It regulates the symbiotic host-microbiome interactions by training and developing the host’s innate and adaptive immunity. This interaction plays a vital role in host defence mechanisms and at the same time, balancing the endogenous perturbations of the ho...
Nostoc commune has abundant bioactive compounds, which are valuable in medical, cosmeceutical, and nutraceutical fields. Currently, the demand for native bioactive compounds in the cosmetic industry is greater than ever, especially with regard to algae extracts. In this study, we evaluated the biochemical compounds, safety, and effect on wound-heal...
Toll-like receptors (TLRs) are evolutionarily conserved proteins of pattern recognition receptors (PRRs) and play a crucial role in innate immune systems recognition of conserved pathogen-related molecular samples (PAMPs). We identified and characterized TLR18 from Nile tilapia (Oreochromis niloticus), OnTLR18, to elucidate its role in tissue expre...
R-phycoerythrin (R-PE), a pigment complex found in red algae, was extracted and purified from a newly identified red alga, Colaconema formosanum, and its bioactivities were examined. It was revealed that R-PE treatment resulted in high cell viability (>70%) to the mammalian cell lines NIH-3T3, RBL-2H3, RAW264.7, and Hs68, and had no effect on cell...
Simple Summary
In this study, we found that phycoerythrin from Colaconema sp. can differentially stimulate the immune response of whiteleg shrimp in vitro and in vivo and could potentially be used as an immunomodulator in shrimp culture.
Abstract
We investigated whether phycoerythrin (PE), a pigment sourced from marine algae, could act as an immun...
This study investigated the effects of guava leaf extract (GLE) on immune responses, growth performance, and resistance to Vibrio parahaemolyticus in white shrimp (Penaeus vannamei). To examine the effect of GLE on the immune response of white shrimps, they were treated with various concentrations of GLE on hemocyte (in vitro) and were orally admin...
In this study, climbing perch larvae were administrated with different diet treatments: Artemia nauplii enriched with 10⁸ CFU mL⁻¹ L. lactis (LA); nauplii enriched with 0.5 g L⁻¹ sodium alginate (SA); nauplii enriched with 0.5 g L⁻¹ sodium alginate containing 10⁸ CFU mL⁻¹ L. lactis (LSA); and non-enriched nauplii as the control (A). Throughout the...
Phycoerythrin (PE) is a compound with strong potential for both basic research and industrial applications, but short supply and high prices have so far hindered its development. One common problem is a shortage of biomass for extraction. The aim of the present study was to determine a cultivation strategy (optimizing temperature, irradiance, photo...
Toll/interleukin 1 receptor domain-containing adaptor protein (TIRAP) and toll/interleukin 1 receptor-domain-containing adapter-inducing interferon-β (TRIF) are crucial adaptors of signal transduction for the signaling pathways of toll-like receptors (TLRs). TIRAP and TRIF perform an essential function in an antimicrobial immune response; however,...
This study investigates the effects of Phyllanthus amarus extract (PAE) on immune responses, growth, and resistance to Vibrio alginolyticus in white shrimp (Litopenaeus vannamei). In vitro PAE treatment did not alter the cell viability of haemocytes and significantly enhanced immune parameters such as phenoloxidase (PO) activity, phagocytic activit...
Myeloid differentiation factor 88 (MyD88) is an indispensable component of the toll-like receptor (TLR)- and interleukin 1 receptor-mediated signaling pathways and plays an important role in defensive immune responses. In this study, we cloned MyD88 from the Nile tilapia, Oreochromis niloticus (OnMyD88). Phylogenetic analysis showed that OnMyD88 is...
This study investigates the effects of three medicinal herbal extracts, namely Bidens pilosa (BPE), Lonicera japonica (LJE), and Cyathula officinalis (COE), on nonspecific immune parameters of cobia (Rachycentron canadum) in vitro and in vivo. During in vitro tests, BPE treatment increased reactive oxygen species (ROS) production in a dose-dependen...
This study was to examine the immunomodulatory effects of oxytetracycline (OTC) on the white shrimp Litopenaeus vannamei (Boone, 1931). Immune parameters were not suppressed by OTC treatment in haemocytes in vitro . A total of 96 healthy shrimp were divided into four groups. Group 1 was the control group. Group 2 received 50 mg OTC/kg body weight/d...
The objective of this study was to investigate the effects of Caulerpa racemosa (Agardh, 1873) polysaccharides (CRP) on non-specific immunity of the white shrimp, Litopenaeus vannamei (Boone, 1931). The extracted polysaccharide consists of galactose, glucose, and mannose. Total polyphenolic compound and total flavonoids content in CRP were determin...
The Nile tilapia (Oreochromis niloticus) is one of the major food fish species produced in tropical and subtropical regions. However, this industry has been facing significant challenges from microbial infections. Understanding how hosts initiate immune responses against invading microbes is the first requirement for addressing disease outbreak pre...
The white shrimp Litopenaeus vannamei is one of the most commercially important aquatic species. However, intensive farming to meet economic demands has been linked to animal stress and subsequent disease outbreaks. In this study, we explore the immunomodulatory effects of Sargassum horneri hot-water extract (SHE) on the immune parameters of L. van...
This study was conducted to examine the combinatory effects of β-glucan and oxytetracycline (OTC) on hybrid giant tiger groupers (Epinephelus fuscoguttatus × Epinephelus lanceolatus). In in vitro tests, OTC significantly reduced superoxide anion production and phagocytic activity in primary head kidney leukocytes. However, this suppressive effect w...
The acute phase response (APR) is an early innate immune function that is initiated by inflammatory signals, leading to the release of acute phase proteins to the bloodstream to re-establish homeostasis following microbial infection. In this study we analysed the Atlantic salmon (Salmo salar) whole-genome database and identified five C-reactive pro...
Fish type I IFNs are classified into two groups with two (group I) or four (group II) cysteines in the mature peptide and can be further divided into four subgroups, termed IFN-a, -b, -c, and -d. Salmonids possess all four subgroups, whereas other teleost species have one or more but not all groups. In this study, we have discovered two further sub...
Mammalian Toll-like receptor (TLR) 7 and 8 are responsible for recognizing viral single-stranded RNA (ssRNA) and are activated by anti-viral imidazoquinoline compounds, leading to a series of defensive mechanisms being launched to protect the host against viruses. In this study, we identified two TLR7 (with one probably a pseudogene) and three TLR8...
Questions
Questions (2)
I found that when people do dual luciferase assay, they transfected construct and using empty vector to make all the transfected DNA amount are the same (eg. cells were transfected with either x μg recombinant plasmid or the control plasmid, plus y μg of the reporter plasmid, made up to a total of z μg plasmid DNA per well with empty vector). I am wondering if I can just use same amount of construct without empty vector, or should I concern about putting empty vector?
I have done head kidney (HK) primary cell culture from Atlantic salmon (post smolt) before. I use 1.5 ml TRI reagent (Sigma) to extract RNA from 2 million primary HK leukocytes, following DNase treatment (Ambion) and reverse transcription for cDNA, this method gave me good Cp value from housekeeping gene (EF-1a and b-actin, Cp=12~13). Hundreds of samples were done and no problems were observed.
However, I did the same procedure in primary spleen cells (I used percoll to separate from red blood cell, wash 3 times with fresh medium after centrifuge) and I got EF-1a Cp= 20. I also obeserved 1 million cells gave me the best cDNA (EF-1a=20), but it went worse when I put more or less cells (4,2, 0.5, 0.25 million). But I can obtain good Cp value (EF-1a = 12) from the spleen tissue sample (also salt water salmon) in RNAlater (also use TRI reagent).
Now I will first try RNA extraction kit from Bioline and see how it goes with kit compare to TRI reagent method. I am wondering if anyone have similar experience and could suggest me what can I do to improve my cDNA quality.
