Piotr Wasąg

Kazimierz Wielki University in Bydgoszcz · Department of Biochemistry and Cell Biology

Pollen tube growth depends on several complex processes, including exo/endocytosis, cell wall biogenesis, intracellular transport, and cell signaling. Our previous results provided evidence that calreticulin (CRT)—a prominent calcium (Ca2+)-buffering molecular chaperone in the endoplasmic reticulum (ER) lumen—is involved in pollen tube formation an...

Background Pollen development in the anther in angiosperms depends on complicated cellular interactions associated with the expression of gametophytic and sporophytic genes which control fundamental processes during microsporo/gametogenesis, such as exo/endocytosis, intracellular transport, cell signaling, chromatin remodeling, and cell division. M...

Tropomyosin is a two-chain coiled coil protein, which together with the troponin complex controls interactions of actin with myosin in a Ca2+-dependent manner. In fast skeletal muscle, the contractile actin filaments are regulated by tropomyosin isoforms Tpm1.1 and Tpm2.2, which form homo- and heterodimers. Mutations in the TPM2 gene encoding isofo...

Calreticulin (CRT) is a multifunctional resident endoplasmic reticulum (ER) luminal protein implicated in regulating a variety of cellular processes, including Ca⁠2+ storage/mobilization and protein folding. These multiple functions may be carried out by different CRT genes and protein isoforms. The plant CRT family consist of three genes: CRT1 and...

Calcium (Ca(2+)) plays essential roles in generative reproduction of angiosperms, but the sites and mechanisms of Ca(2+) storage and mobilization during pollen-pistil interactions have not been fully defined. Both external and internal Ca(2+) stores are likely important during male gametophyte communication with the sporophytic and gametophytic cel...

Main conclusion: Calreticulin is involved in stabilization of the tip-focused Ca (2+) gradient and the actin cytoskeleton arrangement and function that is required for several key processes driving Petunia pollen tube tip growth. Although the precise mechanism is unclear, stabilization of a tip-focused calcium (Ca(2+)) gradient seems to be critica...

The nuclear matrix (NM), or nuclear skeleton, is the non-chromatin, ribonucleoproteinaceous framework that is resistant to high ionic strength buffers, nonionic detergents, and nucleolytic enzymes. The NM fulfills a structural role in eukaryotic cells and is responsible for maintaining the shape of the nucleus and the spatial organization of chroma...

The phytohormone abscisic acid (ABA) coordinates plant responses to stressors such as drought, extreme temperature and high salinity, as well regulates non-stress responses including seed formation and maturation, seed and bud dormancy, root growth, leaf senescence, and transition between vegetative and reproductive growth. ABA, similarly like the...

Abscisic acid (ABA) regulates a diverse array of processes including seed maturation and dormancy, root growth, leaf senescence, and the transition between vegetative and reproductive growth. ABA is also a major stress hormone that regulates the adaptation of plants to environmental stresses including drought, cold and salt. It controls the stomata...