Piotr Waligórski

Piotr Waligórski
Franciszek Górski Institute of Plant Physiology · Department of Developmental Biology

Doctor of Philosophy

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72
Publications
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1,040
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Publications

Publications (72)
Article
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Culinary rhubarb is grown for its large, thick leaf petioles. Red-stalked cultivars and selection are more attractive for the fresh market and processing industry. In vitro cultures are important for rapidly multiplying value genotypes and producing plants free of viruses. This study aimed to develop an in vitro propagation method for six rhubarb s...
Article
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Plant biotechnology creates opportunities for the cultivation of plants regardless of their natural habitats, which are often protected or difficult to access. Maintaining suspension cell cultures in bioreactors is an advanced part of biotechnological research that provides possibilities for obtaining plant tissue on a large scale. In this study, t...
Article
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Water deficit affects the growth as well as physiological and biochemical processes in plants. The aim of this study was to determine differences in physiological and biochemical responses to drought stress in two wheat cultivars—Chinese Spring (CS) and SQ1 (which are parents of a mapping population of doubled haploid lines)—and to relate these res...
Article
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This research’s scope encompassed biotechnological, phytochemical, and biological studies of Schisandra henryi, including investigations into its in vitro microshoot culture grown in PlantForm bioreactors (temporary immersion systems, TISs), as well as extracts from leaves of the parent plant, focusing on anti-inflammatory, antioxidant, anticancer,...
Article
Aims Due to the lack of experimental databases, together with the chemical complexity and the dynamic nature of plants' metabolome, most of the metabolites in complex biological materials (like plant in vitro tissue cultures) are not‐annotated, unidentified metabolites. In this study, a method for further metabolite characterization and classificat...
Article
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Drought is one of the major threats to food security. Among several mechanisms involved in plant stress tolerance, one protein family—the plant metallothioneins (MTs)—shows great promise for enhancing drought resistance. Plant metallothioneins in oat (Avena sativa L.) have not yet been deeply analysed, and the literature lacks a comprehensive study...
Article
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Abstract: Light-emitting diodes (LEDs) have emerged as efficient light sources for promoting in vitro plant growth and primary and secondary metabolite biosynthesis. This study investigated the effects of blue, red, and white-red LED lights on plant biomass growth, photosynthetic pigments, soluble sugars, phenolic compounds, the production of Amary...
Article
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Dormancy development in micropropagated plantlets at the acclimatization stage and early growth ex vitro is undesirable as it lowers their survival rate and restricts the efficient year-round production of planting material. Thus far, little is known about the factors and mechanisms involved in the dormancy development of micropropagated herbaceous...
Article
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Bacteria and fungi that are able to metabolize steroids express 3-ketosteroid-Δ1-dehydrogenases (KstDs). KstDs such as AcmB form Sterolibacterium denitrificans Chol-1 catalyze the enantioselective 1α,2β-dehydrogenation of steroids to their desaturated analogues, e.g., the formation of 1,4-androstadiene-3,17-dione (ADD) from 4-androsten-3,17-dione (...
Article
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Culinary rhubarb is a vegetable crop, valued for its stalks, very rich in different natural bioactive ingredients. In commercial rhubarb stalk production, the bud dormancy development and release are crucial processes that determine the yields and quality of stalks. To date, reports on rhubarb bud dormancy regulation, however, are lacking. It is kn...
Article
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The aim of the study was to examine combinations of base oils and herbal additives with a view to obtaining macerates with improved health benefits. Base oils were cold-pressed from the seeds of black cumin, borage, evening primrose, safflower, walnut, common hazel, and oilseed rape, as well as the flesh of sea-buckthorn fruits. They were then supp...
Article
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Rumex thyrsiflorus Fingerh. is mentioned as a European folk medicinal plant. This species has also been traditionally used as an edible plant in Eastern Europe because of its nutritional value. During the study, qualitative and quantitative sex-related differences of phenolic constituents in methanolic leaf extracts of R. thyrsiflorus were evaluate...
Article
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The aim of the present study was to evaluate the effect of osmotic stress caused by polyethylene glycol (PEG) 6000 in hydroponic culture on wheat seedlings of drought-resistant Chinese Spring (CS) and drought-susceptible SQ1 cultivar, and to examine the alleviative role of exogenous polyamines (PAs) applied to the medium. The assessment was based o...
Article
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Ten doubled haploid (DH) lines of winter barley with an increased range of freezing/drought tolerance were used to identify phytohormones involved in plant stress acclimation. Cold hardening and drought stress were applied at the most critical stages of plant development on young seedlings and heading plants, respectively. The level of the phytohor...
Article
Full-text available
In this work we analyzed the quaternary structure of FAD-dependent 3-ketosteroid dehydrogenase (AcmB) from Sterolibacterium denitrificans, the protein that in solution forms massive aggregates (>600kDa). Using size-excursion chromatography (SEC), dynamic light scattering (DLS), native-PAGE and atomic force microscopy (AFM) we studied the nature of...
Article
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In this article, the effects of cold on the development of Lupine angustifolius and the possibility of mitigating it, via seed hydropriming or pre-treatment with butenolide (10−6 M–10−4 M), are investigated in two cultivars, differing in their ability to germinate at low temperature. Physiological background of plant development after cold stress w...
Article
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Can dust and tarry substances that are rich in polycyclic aromatic hydrocarbons (PAHs), which are deposited on leaves, cause changes in the water retention of tree crowns? It is hypothesized that the contact angle between droplets and a leaf's surface and water capacity changes because of the content of PAHs. This angle is treated as a bioindicator...
Article
The study was focused on the influence of salicylic acid (SA) on maize seeds germination and on some physiological and biochemical processes in maize plants growing in the hydroponic culture under copper (Cu) stress. A significant influence of SA pretreatment on the advanced induction of the maize seeds metabolic activity and the level of the endog...
Article
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We investigated the modification of cold-induced mechanisms of photosynthetic apparatus adjustment and phytohormone response by brassinosteroid 24-epibrassinolide (EBR) and its consequences for frost tolerance of perennial ryegrass (Lolium perenne L.). We recorded the responses of two cultivars with contrasting frost tolerances to foliar hormone ap...
Article
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Polyamines (PAs) are related to many physiological processes, including soil drought stress. Two yellow lupin ‘Morocco 4’ (drought tolerant) and ‘Taper’ (drought sensitive) were exposed to soil drought for 2 weeks. The half of the examined plants were additionally sprayed with a solution of polyamine biosynthesis inhibitor—dl-α-difluoromethylargini...
Article
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Somatic embryogenesis (SE) of Cyathea delgadii presents a model system for investigating the mechanisms associated with the acquisition of embryogenic competence by single epidermal cells of stipe explants cultured on plant growth regulator-free medium. The present work reveals relationship between endogenous hormone and sugar content in the proces...
Article
This paper describes experiments concerning improvement of drought tolerance in narrow-leafed lupine (Lupinus angustifolius L.) by maternal effects. The first step involved harvesting seeds from plants exposed and not exposed to drought during flowering and seed ripening. The next-generation plants grown from these seeds were exposed to drought and...
Article
The pattern of changes in some phytohormones content and oxidoreductive processes during seed germination of two lupine species was studied. During radicle protrusion (day 2), the amount of cytokinins in narrow-leafed lupine seeds greatly increased in both radicles and cotyledons, and it was accompanied with a huge drop in abscisic acid. In white l...
Technical Report
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In coupled techniques, resolution sensitivity of the analytical method and mass spectrometry for identification of the separated components, are used. One of the analytical techniques which enjoys growing interest of the environmental laboratories, natural or medical diagnostics, is the liquid chromatography coupled with mass spectrometry. The most...
Article
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Brassinosteroids (BR) are plant steroid hormones that were discovered more than thirty years ago, but their physiological function has yet to be fully explained. The aim of the study was to answer the question of whether/how disturbances in the production of BR in barley affects the plant's metabolism and development under conditions of optimal wat...
Article
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Forestry works using chainsaws result in up to 7 million liters of various mineral oils being soaked annually into forest soils. These substances, containing a complex mixture of polycyclic aromatic hydrocarbons (PAHs), are highly toxic. The aim of the study was to determine the effect of oil contamination with PAHs on the physical and biological p...
Article
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Hemangiosarcoma (HSA) is a common and highly metastatic tumor that affects predominantly large-breed dogs. The median survival time for dogs diagnosed with splenic HSA without adjuvant chemotherapy after surgery is 86 days. Fenofibrate is an FDA-approved drug commonly utilized to treat high cholesterol and high triglyceride levels in humans. Howeve...
Article
Auxin is involved in a wide spectrum of physiological processes in plants, including responses controlled by the blue light photoreceptors phototropins: phototropic bending and stomatal movement. However, the role of auxin in phototropin-mediated chloroplast movements has never been studied. To address this question we searched for potential intera...
Article
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Fenofibrate, a well-known normolipidemic drug, has been shown to exert strong anticancer effects against tumors of neuroectodermal origin including glioblastoma. Although some pharmacokinetic studies were performed in the past, data are still needed about the detailed subcellular and tissue distribution of fenofibrate (FF) and its active metabolite...
Article
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The aim of the study was to determine the effect of exogenous sucrose and cytokinin on ethylene production and responsiveness in relation to the shoot formation of Pelargonium x hortorum 'Bergpalais' in vitro. Increasing the concentration of sucrose from 15 to 40 g L-1 in medium containing meta-topolin (mT) resulted in a two-fold decrease in the nu...
Poster
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The 1st International Symposium regarding the Effects of Irrigation and Drainage on Rural and Urban Landscapes (IRLA2014) is organised in the framework of “Efficient Irrigation Management Tools for Agricultural Cultivations and Urban Landscapes (IRMA)” project (http://www.irrigation-management.eu/) which is financed by the European Territorial Coop...
Article
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DNA-bound Hoechst 33258 is readily excited with UV light and emits blue fluorescence, however, upon exposure to UV, the dye undergoes photobleaching as well as photoconversion to a blue-excited green-emitting form. We demonstrate that the UV-generated green-emitting form of Hoechst 33258 exhibits spectral properties very similar to the form of the...
Article
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Key message: Effective microspore embryogenesis in triticale is determined by a specific hormonal homeostasis: low value of IAA/cytokinins, IAA/ABA and cytokinins/ABA ratios as well as proper endogenous/exogenous auxin balance, which favours androgenic structure formation and green plant regeneration ability. The concentration of plant growth regu...
Article
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The effect of soil drought (10 days) on the growth of plants, the accumulation of water and leakage of electrolytes, gas exchange, the contents of chl a + b and carotenoids in leaves and photochemical activity of photosystem II was studied at the seedling stage by transient fluorescent analysis in 20 of the popular varieties of polish spring wheat....
Article
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Polycyclic Aromatic Hydrocarbons (PAHs) are the products of incomplete combustion of organic materials, which are present in cigarette smoke, deep-fried food, and in natural crude oil. Since PAH-metabolites form DNA adducts and cause oxidative DNA damage, we asked if these environmental carcinogens could affect transforming potential of the human P...
Article
Full-text available
The aim of the study was to assess the role of salicylic acid (SA) and abscisic acid (ABA) in osmotic stress tolerance of wheat seedlings. This was accomplished by determining the impact of the acids applied exogenously on seedlings grown under osmotic stress in hydroponics. The investigation was unique in its comprehensiveness, examining changes u...
Article
The growth response and antioxidant capacity of Brassica oleracea var. capitata f. alba plants treated with 70ppb of ozone was examined. Four week old cabbage seedlings were fumigated with O3 for 3 days before being transplanted into the growing field. The effect of O3 treatment was determined directly after fumigation and over the course of field...
Article
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The physiological reasons for the differences in sensitivity of C3 and C4 plant species to environmental stresses have not been thoroughly explained. In this study the effects of drought stress on the growth and selected physiological traits were examined in the seedlings of 13 single cross maize (C4 plant) hybrids and 11 spring triticale (C3 plant...
Article
Differences in the activity of superoxide dismutase, catalase (CAT) and ascorbate peroxidase (APX) as well as in the concentration of ascorbate, tocopherol and hydrogen peroxide (H₂O₂) were found in leaves from different layers of the Chinese cabbage (Brassica pekinensis (Lour.) Rupr.) head. The youngest chlorophyll-deficient leaves from the most i...
Article
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Cisplatin is one of the most widely used and effective anticancer drugs against solid tumors including cerebellar tumor of the childhood, Medulloblastoma. However, cancer cells often develop resistance to cisplatin, which limits therapeutic effectiveness of this otherwise effective genotoxic drug. In this study, we demonstrate that human medullobla...
Article
Self-incompatibility (SI) of white cabbage (Brassica oleracea var. capitata), a biological mechanism triggering the ability to pollinate plant ovules, is modelled based on the statistical analysis of electrophoretic patterns of stigma extracts. The patterns obtained for 72 plants grown in three different seasons (2003-2005) have been explored with...
Article
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Arnica montana is a rare plant that needs special protection because of its intensive harvesting for medicinal purposes. The present work was aimed at finding optimal culture conditions for Arnica plants in order to enable their successful reintroduction into their natural stands. Plants were cultivated under controlled greenhouse conditions on sub...
Article
We investigated changes in concentrations of abscisic (ABA) and salicylic acid (SA), phenolic compounds and phenylalanine ammonia-lyase (PAL) activity in relation to cold-induced tolerance of four androgenic genotypes of Festulolium (Festuca × Lolium hybrids) to frost and to the snow mould fungus Microdochium nivale. Cold acclimation increased fros...
Article
Full-text available
The optimization of the analytical buffer composition for micellar electrokinetic chroma- tography in order to make simple separations of 10-deacetylbaccatine III from sample matrices (other taxanes and other compounds) and to determine its concentration in the samples was performed. The samples were prepared from crude Taxus baccata L. needle ex-...
Article
The effects of ‘partial root-zone drying’ (PRD), compared with full irrigation (FI) and deficit irrigation (DI), on soil and plant water status, plant growth and water use efficiency (WUE) were investigated in potted tomatoes (Lycopersicon esculentum L., var. Cedrico) at the early fruiting stage. In FI the whole root system was irrigated daily clos...
Article
Full-text available
Previously published results showed that high relative reduction state of PSII (PSII excitation pressure) during both early seedling growth (prehardening) as well as cold deacclimation caused significant changes in growth pattern. The differences in elongation growth rate were related to the cold acclimation of photosynthetic apparatus and to frost...

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