Piotr Gomułka

Piotr Gomułka
University of Warmia and Mazury in Olsztyn · Department of Ichthyology

PhD

About

58
Publications
13,770
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920
Citations
Citations since 2016
16 Research Items
506 Citations
2016201720182019202020212022020406080
2016201720182019202020212022020406080
2016201720182019202020212022020406080
2016201720182019202020212022020406080

Publications

Publications (58)
Article
The influence of the implantation of 12 mm passive integrated transponders (PIT) tags on juvenile brown trout (fork length approximately 19 cm) for 28 days after tagging was analyzed. The fish were divided into three groups: control, sham (injected with a syringe without PIT tag insertion), PIT tagged. Biological data were collected on days 0 (init...
Article
Controlled indoor reproduction of European eel is commonly recognized as a prospective tool to prevent extinction of this fish species. There is a little information about diversity of bacterial microflora of European eel during long maturation under controlled but changing thermal and salinity conditions. Characteristics of the composition of the...
Article
Full-text available
This study aimed to assess the effect of constant darkness applied to fish during controlled breeding on reproductive traits in domesticated females of Eurasian perch. Based on the assumption that keeping fish in constant darkness during the reproduction operation may reduce stress, suspected to be responsible for variable spawning effectiveness in...
Article
Full-text available
This study aimed to determine the impact of immersing pikeperch (body weight approximately 94 g) in an aqueous solution of sodium chloride on gasometric, hematological, and biochemical indicators. The impact of the sodium chloride concentration (NaCl 10, 15, and 20 g L⁻¹), immersion time (30 or 60 min) and time of blood sampling (0 h – blood drawn...
Article
Full-text available
To assess the effects on rainbow trout reproduction of feed contamination with zearalenone (ZEN) at the current European guidance value (2 mg∙kg⁻¹ feed), we performed a life-cycle feeding trial under aquaculture conditions. The trial began when the fish larvae commenced external feeding and continued for 96 weeks, up to the point when the fish reac...
Article
Full-text available
K e y w o r d s: Oncorhynchus mykiss, Amaranthus cruentus, cholesterol, squalene. A b s t r a c t The aim of the study was the assessment of the effect of amaranth meal on serum cholesterol in rainbow trout Oncorhynchus mykiss. Two experimental feeds contained 10.0% and 20.0% of amaranth meal and control feed were prepared. All diets were formulate...
Poster
The study aimed to assess the effects of natural dietary immunostimulant addition on survival, growth, digestive enzyme activity and immune system response of river trout (Salmo trutta m. fario). The experimental rearing lasted 28 days and it took place in The Stanisław Sakowicz Inland Fisheries Institute, in the research facility in Rutki, Poland....
Article
Full-text available
K e y w o r d s: Oncorhynchus mykiss, Amaranthus cruentus, liver histology, hematology, blood biochemistry, anti-nutritional factors. A b s t r a c t The effect of dietary inclusion of amaranth meal on hematology and blood biochemistry, of rainbow trout Oncorhynchus mykiss was examined. The fish (mean length 35.2 ± 0.6 cm; mean weight 524.8 ± 28.5...
Article
Impact statement: The delivery of short snippets of RNA, such as synthetic miRNA agents, is an essential step for achieving RNA-mediated knockdown, which has not been studied in sufficient detail in fish. Our results indicate that a MiR92b-3p mimic may be effectively delivered via intraperitoneal injection to the spleen and the liver of whitefish,...
Article
To investigate the effects of feed contamination with zearalenone (ZEN) at the current European Commission (EC) guidance value (2 mg⋅kg⁻¹ feed) on the growth and health of rainbow trout, we performed a long-term feeding trial under aquaculture conditions. It started with the external feeding of the fish larvae, and continued for 96 weeks, at which...
Article
Full-text available
The aim of this study was to investigate anesthetic potency and acute toxicity of 2-phenoxyethanol (2PE) and 1-phenoxy-2- propanol (PP) for common carp. During efficacy tests, individual phases of anesthesia and recovery, as well as behavior and survival rates, were determined. As effective time for anesthesia and recovery, 3 and 10 min respectivel...
Article
Full-text available
1-phenoxy-2-propanol is a common and effective anaesthetic for aquatic organisms such as bivalves and pulmonates. However, there are no data regarding its influence on fish organisms. In the present study the anaesthetic efficacy of 1-phenoxy-2-propanol and its influence on biochemical and haematological blood indices of juvenile common carp were s...
Article
Full-text available
The increased interest in culinary heritage as an integral part of the identity of the country is observed in Poland recently. Current students will probably shape the future “culinary consciousness” of Poles, therefore studies aimed at understanding the opinions and attitudes of students towards traditional Polish food were undertaken. The researc...
Article
To date, there has been no systematic approach comprehensively describing the sequence of pathological changes in fish during prolonged exposure to microcystin-LR (MC-LR). Towards this aim, juvenile whitefish individuals received an intraperitoneal injection with pure MC-LR, and the injection was repeated every week to maintain continuous exposure...
Article
Full-text available
The aim of the study was to compare the share of hatchery-reared lake trout Salmo trutta m. lacustris in catches of fingerlings in the upper Wda and the Pilica Rivers in Autumn, following the stocking fish in spring 2005. Larvae in number of 40 thousand had been previously exposed to the thermal shock procedure (temperature decrease from 8.0 to 2.5...
Article
Full-text available
The aim of the this study was the to assessment of use the amaranth meal in feed for rainbow trout Oncorhynchus mykiss. Two experimental feeds contained 5.0% and 10.0% of amaranth meal were prepared and compared to reference diet (commercial feed) containing similar amounts of specific nutrients. Initial body wet weight of experimental trout was 52...
Article
To gain insight into the possible implications for aquaculture of the presence of ZEN in fish feed at the EC guidance level, we determined the effects of over two months of feeding of premarket size rainbow trout (~ 250 g) with ZEN-contaminated feed at a dose of 1.81 mg·kg− 1 feed (90.5% of the current guidance value) under aquaculture conditions....
Article
The aim of this study was to compare physiological responses in Siberian sturgeon ( Acipenser baeri ) induced by propofol and CO 2 anaesthesia. Two procedures were applied during the experiment. In procedure I, blood samples were collected immediately after exposure (1, 2, 5, 10 min) to the anaesthetic. In procedure II, fish were exposed to the ana...
Article
Full-text available
Propofol, 2,6-diisopropylphenol, seems to be a good candidate as a fish anaesthetic, however, no study regarding propofol influence on fish has yet been reported. The aim of this study was to examine propofol pharmacokinetics in rainbow trout (Oncorhynchus mykiss) following bath exposure. Fish (n = 100) were exposed to an aqueous propofol bath at 1...
Article
Sturgeon production involves unavoidable handling procedures inducing stress response. Clove oil is the anaesthetic commonly used in sturgeon culture to minimise stress and pain. Propofol is one of the most frequently used anaesthetic in humans. Propofol is a promising candidate anaesthetic for fish. The aim of the study was to compare the effect o...
Article
Full-text available
Propofol (2’6-diizopropylofenol) effectively induced general anaesthesia in whitefish in the preliminary test. The aim of the study was to assess the effects of propofol on European whitefish by haematological and biochemical indices of blood plasma. A total of 60 specimens of whitefish from experimental hatchery of Inland Fisheries Institute in Ol...
Article
Full-text available
The effect of the substitution of fish oil with amaranth oil in fish feed on body composition was studied in rainbow trout Oncorhynchus mykiss. Two experimental feeds, containing 7.2% and 5.0% of amaranth oil were used. The experiment was conducted in triplicate for each treatment (n=50). Initial body wet weight of experimental fish was 7.4±0.1 g....
Article
Full-text available
Acute ammonia toxicity was investigated in four developmental stages of the juvenile ide, Leuciscus idus: 1, 10, 20 and 30 days after the first feeding. Mean (±SD) total length of the larvae was 8.5 ± 0.3, 15.7 ± 0.7, 23.0 ± 2.0 and 29.7 ± 2.0 mm, and standard weight was 1.6 ± 0.3, 9.2 ± 5.5, 94.9 ± 31.0 and 196.0 ± 31.7 mg, respectively. The larva...
Article
The aim of this study was to assess the effect of pesticide Fury 10 EW, containing zeta-cypermethrin 100 g.l-1, on common carp (Cyprinus carpio L.). The toxicity tests were performed on common carp according to OECD 203 methodologies. The common carp were exposed to Fury 10 EW at concentrations, 5, 7, 10, 50 and 100 μg.l-1 for 96 h and compared to...
Article
Full-text available
Effect of increased hypolimnion water temperature on stability of thermal profiles in startified lakes. Abstract The results demonstrated a distinct increase in temperature during the summer stagnation in the water layer below 7 meters of depth in the experimental part of the lake. There, the temperature of near-bottom water increased by 5.6⁰C from...
Article
Full-text available
Acute toxicity of ammonia was investigated in four life stages of juvenile chub, Leuciscus cephalus (cyprinid fish): 1, 10, 20 and 30 days after the first feeding. The fish used for the toxicity test were reared intensively in a closed recirculation system. Each acute toxicity test duration was 96 h and lethal concentration LC1, LC50 and LC99 value...
Article
Full-text available
In opposition to toxicology of mammals piscine toxicology is closely connected with the conditions of external environment. The aquatic environment is necessary for embryonic development and after hatching during short or long-lasting larval period of most fish species. An aquatic environment is polluted by many industrial and agricultural wastes....
Article
Full-text available
A preliminary examination was carried out into the effect that various activating liquids have on the survivability of ide Leuciscus idus (L.) embryos. It was found to be similar (54–59%) in four study groups; the embryo survivability was found to have decreased (44%) only in the group where non-sterilised tap water was used for gamete activation....
Article
Full-text available
Laboratory-bred goldfish were experimentally infected with Contracaecum rudolphii, either directly (with nematode larvae) or indirectly via infected zooplankton. Intensity and prevalence of infection were markedly higher in fish exposed to the infected zooplankton than in goldfish exposed to nematode larvae. When transmitted to fish via the zooplan...
Article
Full-text available
A few extraction methods for the reverse-phase high performance liquid chromatography (RP-HPLC) determination of alkylphenolic compounds in fish tissues were examined. The best results of the isolation of 4-nonylphenol (4-n-NP) and 4-tert-octylphenol (4-t-OP) from Rainbow trout were obtained using soxhlet extraction; the recoveries reached 64.64 ±...
Article
Studies on larval rearing of silver bream (Vimba vimba), a migratory cyprinid fish have addressed on limited scale larval and juvenile rearing using commercial and semipurified diets along with live feeds, such as brine shrimp Artemia nauplii. The objectives of the present study were (1) to determine whether experimental, protein-, peptide-, free a...
Article
Common carp spawning characteristics were compared between season and out-of-season periods. There were no differences in the percentage of ovulation, spermatozoa motility and embryo survival to the eyed-egg-stage between the spawning periods. Fish spawned during the season produced oocytes of higher total weight than those spawned out-of-season. O...
Article
The aim of the present study was to assess changes induced in the organism of Siberian sturgeon (Acipenser baerii) by eugenol and MS-222 anaesthesia on the basis of haematological indices, biochemical blood profile values and histological examinations. The haematological and biochemical indices were assessed in fish immediately and 24 h after anaes...
Article
The aim of the present study was to assess changes induced in the organism of Siberian sturgeon (Acipenser baerii) by eugenol and MS-222 anaesthesia on the basis of haematological indices, biochemical blood profile values and histological examinations. The haematological and biochemical indices were assessed in fish immediately and 24 h after anaes...
Article
The present study includes the evaluation of morphological changes in the digestive tract of larval, stomachless fish silver bream (Vimba vimba) fed with various diets – live Artemia nauplii, commercial feed Aglo Norse (NOR) and semi-purified formulated diets: casein–gelatin (CG), dipeptide-protein (50P), dipeptide (100P), no-arginine dipeptide die...
Book
Full-text available
Monography about chosen aspects of artificial breeding of cyprinid (Cyprinidae) reophilic fishes. Chapters: Gears and methods for catching of spawners of cyprinid reophilic fishes; Practical use of electrocatching in surface waters; Transport and manipulations with fishes in controlled conditions; Chosen hormonal treatments used in fish breeding; B...
Conference Paper
Full-text available
The aim of this study was to test different spawning agents in pikeperch artificial reproduction in captivity.
Article
The aim of the study was to investigate the acute toxicity of 2-phenoxyethanol to sheatfish, and using the values of haematological and biochemical profiles of blood and histological tissue examinations to assess the effects of the fish exposure to that anaesthetic. The values of acute toxicity of 2-phenoxyethanol to sheatfish were found to be 10mi...
Book
Full-text available
Manual on artificial reproduction of pikeperch (Stizostedion lucioperca, Percidae): Chapters: 1) Artificial reproduction of pikeperch - organization of the manual, 2) State of art of pikeperch reproduction - a short review, 3) Spawners and handling, 4) Checking maturation stage of females, 5) Spawning agents and their application, 6) Obtaining game...
Article
The population of Great Cormorant (Phalacrocorax carbo (L.)) has rapidly increased in Poland over the last 20 years. We evaluated the diet of this species at seven roost sites and breeding colonies. Food composition was analysed based on pellets and fish regurgitated by birds. Coregonid fishes were recorded in cormorant diets in several colonies; w...
Article
Full-text available
Marking of fish larvae by means of thermal shock is based on the natural mechanism of otolith growth and diurnal formation of rings. A disorder in that mechanism results in the creation of rings that differ visually from others in thickness, which in turn, allows for the identification of individuals that are so tagged. In the case of coregonids, p...
Article
The study aimed at assessing the effects of a pyrethroid cypermethrin on common carp (Cyprinus carpio L.) on the basis of the results of acute toxicity tests and haematological, biochemical and histological examinations. Experimental carp were exposed to the pesticide preparation Alimetrin 10 EM in the concentration of 29.1 microg/l(-1) (96hLC(50))...
Article
The aim of this study was to assess the effect of cypermethrin [(R,S)-α-cyano-3-phenoxybenzyl (1RS)-cis,tra-3-(2,2-dichlorovinyl)-2,2- dimethyl cyclopropane carboxylate] on rainbow trout (Oncorhynchus mykiss). The effect was assessed on the basis of the results of acute toxicity tests and on the comparison of results of haematological, biochemical...
Article
Full-text available
The premise that free amino acid or dipeptide based diets will resolve the nutritional inadequacy of formulated feeds for larval and juvenile fish and improve utilization of nitrogen in comparison to protein-based diets was tested in stomachless fish, common carp (Cyprinus carpio L.) larvae. We examined the postprandial whole body free amino acid (...
Article
Velí‰ek J., T. Wlasow, P. Gomulka, Z. Svobodová, L. Novotn˘, E. Ziomek: Effects of Clove Oil Anaesthesia on European Catfish (Silurus glanis L.). Acta Vet. Brno 2006, 75: 99-106. The aim of the study was to investigate acute toxicity of clove oil for European catfish and, using values of haematological and biochemical profiles of blood and histolog...
Article
Full-text available
Fish should be anaesthetised during any procedure that is stressful or likely to cause pain. Although anaesthetics are widely used an over the world when transporting and handling fish, knowledge about their application is rather negligible in Poland. This paper gives a brief description of anaesthesia in fish. The methods of anaesthesia and proble...
Article
Two myxosporean species: Sphaerospora renicola Dyková et Lom, 1982 and S. molnari Lom, Dyková Pavlaskova et Grupcheva, 1983 were recorded for the first time in Poland. They were found in young carp after first overwintering in cold- and warm-water ponds. Localisation of different developmental stages in carp tissues is described. Differences in fre...
Article
Full-text available
The presence of A. crassus larvae in herring, stickleback, perch, ruffe, pike-perch, bream and roach regurgitated by cormorants from a large breeding colony at Katy Rybackie in Vistula Lagoon, Poland, was investigated. Larvae of nematode were detected only in ruffe, the dominant species in t h e diet of the cormorant (58 % of the weight of fish con...
Article
Full-text available
The causative agents of health problems of rainbow trout from a fish farm were investigated by parasitological and ana- tomo-pathological study of affected fishes. The anatomo-pathological changes in gills, swim bladder, kidney, and especially the deformations of backbone are described. The numerous trophozoites of unidentified species of Myxospore...

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Projects (3)
Project
The continued lack of knowledge concerning the molecular background of adverse effects caused by microcystin-LR (MC-LR) is surprising. This toxin requires additional attention, not only for its ability to cause acute poisoning, but also for its ability to initiate cancer in acute doses, and potentially, to promote cancer via chronic exposure to low concentrations in drinking water. Our recent studies on whitefish (Coregonus lavaretus) revealed that long-term exposure to MC-LR resulted in severe liver injury, followed by regeneration of the liver and its unexpected resilience to further toxin uptake. These effects were accompanied by perturbations of hepatic microRNAs (miRNAs) that have target genes involved in cytoskeletal remodeling, cell metabolism, cell cycle regulation, and apoptosis. Among the most pronounced individual alterations, the reduction of MiR92b-3p expression was the most remarkable, and we suggest roles for the miRNA in the aberrant processes of liver cells. This project addresses potential involvement of MiR92b-3p in the as yet unknown regulatory network of MC-induced hepatotoxicity in fish. After a suite of biochemical, physiological, anatomical, and transcriptomic analyses in vitro and in vivo, we will show how MiR92b-3p works in a damaged liver and which processes it targets. Finally, the research will confirm if and how MiR92b-3p can be targeted therapeutically. We expect it to be shown effective enough to pave a way for its use as a tool for treatment of liver damage in fish. What is more, the RNA-based silencing technique that will be used should yield exciting data for our understanding of the system-level biology of vertebrates.
Project
The growing demand for fish consumption and the limited size of wild fish populations make modern aquaculture an important, sustainable source of the food for the world. Due to the increased use of plant additives in fish feed, there has recently been a considerable growth in attention to problems in modern aquaculture related to the occurrence of undesirable substances of plant-origin in fish feed, including mycotoxins. Although zearalenone (ZEN, a mycotoxin known for hormone-mimicking action) has been found in different fish feeds, there has been no systematic research to conclusively demonstrate what concentration of ZEN may be considered safe for the welfare and reproductive outcome of economically relevant fish species. The virtual lack of data, especially on the long-term effects of low dose exposure of fish to mycotoxins, leaves a serious gap in our knowledge concerning possible threats to aquaculture. Moreover, the European Union’s guidance value for ZEN occurrence in animal feed is not based on hard evidence for any fish species, thus creating a need for complex assessment of this guideline. The aim of this project is to comprehensively assess the possible implications for aquaculture of the current guidance value for the occurrence of the mycotoxin zearalenone in fish feed.