Pinelopi Anagnostopoulou

Pinelopi Anagnostopoulou
University of Cyprus · Medical School

Doctor of Medicine

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56
Publications
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Introduction
Skills and Expertise

Publications

Publications (56)
Article
Objectives: To prospectively quantify at the community level changes in asthma symptom control and other morbidity indices, among asthmatic schoolchildren in response to coronavirus disease 2019 (COVID-19) lockdown measures. Methods: In Spring 2019 and Spring 2020, we prospectively assessed monthly changes in pediatric asthma control test (c-ACT...
Article
Full-text available
Background Specialized clinical care for cystic fibrosis (CF) in Cyprus, a small island country, has been implemented since the 1990s. However, only recently, a national CF patient registry has been established for the systematic recording of patients’ data. In this study, we aim to present data on the epidemiological, genotypic and phenotypic feat...
Preprint
Full-text available
Background Specialized clinical care for cystic fibrosis (CF) in Cyprus, a small island country, has been implemented since the 1990s. However, only recently, a national CF patient registry has been established for the systematic recording of patients’ data. In this study, we aim to present data on the epidemiological, genotypic and phenotypic feat...
Article
Full-text available
Between March and April 2020, Cyprus and Greece health authorities enforced three escalated levels of public health interventions to control the COVID-19 pandemic. We quantified compliance of 108 asthmatic schoolchildren (53 from Cyprus, 55 from Greece, mean age 9.7 years) from both countries to intervention levels, using wearable sensors to contin...
Article
We aimed to determine genetic diagnosis in the national primary ciliary dyskinesia (PCD) cohort of Cyprus, an island with a high disease prevalence. We used targeted next‐generation sequencing (NGS) of 39 PCD genes in 48 patients of Greek‐Cypriot and other ancestries. We achieved molecular diagnosis in 74% of the unrelated families tested. We ident...
Preprint
Full-text available
Between March and April 2020, Cyprus and Greece health authorities enforced three escalated levels of public health interventions to control the COVID-19 pandemic. We quantified compliance of asthmatic schoolchildren from both countries to intervention levels, using wearable sensors to continuously track personal location and physical activity. Cha...
Article
Full-text available
Introduction The multiple breath nitrogen washout (MBW) test offers a sensitive measure of airway function. In this study we aim to 1) assess the validity of the EasyOne Pro® LAB (MBWndd) in an in vitro lung model, 2) assess the feasibility, repeatability and reproducibility of MBWndd and 3) compare outcomes with the Exhalyzer D (MBWEM) and body pl...
Article
Background The nitrogen multiple breath washout (N 2 MBW) technique is increasingly used to assess the degree of ventilation inhomogeneity in school-aged children with lung disease. However, reference values for healthy children are currently not available. The aim of this study was to generate reference values for N 2 MBW outcomes in a cohort of h...
Article
Full-text available
The multiple-breath washout (MBW) is a lung function test that measures the degree of ventilation inhomogeneity (VI). The test is used to identify small airway impairment in patients with lung diseases like cystic fibrosis. However, the physical and physiological factors that influence the test outcomes and differentiate health from disease are not...
Article
Objective: To test whether low variability of tidal volume (VT) and capnographic indices are predictive of subsequent respiratory morbidity in preterm infants. Study design: In a birth cohort of 133 preterm infants, lung function was performed at 44 weeks postmenstrual age. Associations between the coefficient of variation (CV) of VT (CVVT) and...
Article
Full-text available
Children with frequent respiratory symptoms in infancy have an increased risk for later wheezing, but the association with symptom dynamics is unknown. We developed an observer-independent method to characterise symptom dynamics and tested their association with subsequent respiratory morbidity. In this birth-cohort of healthy neonates, we prospect...
Article
Lung clearance index (LCI) is the main outcome of the multiple-breath washout (MBW) test. Current recommendations for LCI acquisition are based on low-grade evidence. The aim of this study was to challenge those recommendations using alternative methods for LCI analysis. Nitrogen MBW measurements from school-aged children, 20 healthy controls, 20 w...
Article
Background: Lung impairment in cystic fibrosis (CF) starts in infancy. However, tools to monitor early lung disease are limited. Respiratory rate (RR) as a key vital sign is easy to assess during sleep and is elevated during acute respiratory disease. Thus, elevated RR could indicate early lung impairment and potentially serve as a diagnostic tool...
Article
Background: Inhaled Corticosteroids (ICS) are the cornerstone of asthma management in pediatric patients. However, in some cases, asthma is not adequately controlled on ICS alone. Long-acting beta2-agonists (LABA) are one of the available additional therapies but their use has rarely been studied among children younger than 5 years. Objective: T...
Article
Background: Capnography via a CO2/O2 nasal cannula is commonly used for respiratory monitoring during sedation. However, signal disturbances are frequently encountered, especially in young children. Objective: Sampling ports placed closer to the trachea have been shown to result in improved signal quality. In a manikin model of a 6-month-old inf...
Article
Introduction: Multiple breath washout (MBW) is a sensitive test to measure lung volumes and ventilation inhomogeneity from infancy on. The commonly used setup for infant MBW, based on ultrasonic flowmeter, requires extensive signal processing, which may reduce robustness. A new setup may overcome some previous limitations but formal validation is...
Article
It is not known at what age lung function impairment may arise in children with cystic fibrosis (CF). We assessed lung function shortly after birth in infants with CF diagnosed by newborn screening. We performed infant lung function measurements in a prospective cohort of infants with CF and healthy controls. We assessed lung clearance index (LCI),...
Article
Background: Multiple breath washout (MBW) is a lung function test that identifies the degree of ventilation inhomogeneity (VI) in the lungs. In vitro validation of MBW devices is recommended. So far, plastic lung models for MBW validation ignored variable degrees of VI. Our primary aim was to create a plastic lung model applicable for physiologica...
Article
Background: In preschool children, measurement of airway resistance using interrupter technique (Rint) is feasible to assess the degree of bronchial obstruction. Although some studies measured Rint in infancy, values of Rint and its variability in preterm infants are unknown. In this study, Rint and its variability was measured at infancy and comp...
Article
Exhaled nitric oxide (FENO) is a well-known, non-invasive airway biomarker. In patients with Cystic Fibrosis (CF) FENO is decreased. To understand if reduced FENO is primary related to Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) dysfunction or an epiphenomenon of chronic inflammation, we measured FENO in 34 infants with CF prior to c...
Article
Interrupter technique (Rint) measures airway resistance during tidal breathing and is thus attractive for preschool children and infants. Repeatability and variability in preterm infants have however never been studied. We assessed repeatability and variability of Rint in healthy term and preterm infants during quiet sleep at five weeks adjusted ag...
Article
Background: An increase in the incidence of asthma among children < 5 years has been identified. GINA guidelines recommend treatment of some asthmatics in this age group with the combination of an inhaled corticosteroid and a long-acting β2-agonist even though this practice has seldom been studied among children younger than 5 years. Aim: To analyz...
Article
Background: Fraction of exhaled nitric oxide (FeNO) is a biomarker of airway inflammation. Elevated FeNO measured in high-risk populations during early childhood is associated with school age asthma. It is unknown if assessment of FeNO shortly after birth is associated with asthma. Aim: We aimed to identify if postnatal, premorbid assessment of FeN...
Conference Paper
Exhaled nitric oxide fraction ( F eNO) is a noninvasive biomarker that is elevated in subjects with asthma and allergic diseases [1]. Nitric oxide (NO) is produced by three NO synthase (NOS) enzymes: neuronal, endothelial and inducible (iNOS), all present in the human lung [2]. The increase of F eNO in asthmatic patients has mainly been attributed...
Article
Background: Multiple-breath washout (MBW) is increasingly used for infant lung function testing. Current guidelines recommend calculating lung clearance index (LCI) and functional residual capacity (FRC) at 2.5% of normalized tracer gas concentration, without clear recommendation for moment ratios (MR). Whether the 2.5% cut-off has the highest dis...
Article
Infant multiple breath washout (MBW) testing serves as a primary outcome in clinical studies. However, it is still unknown whether current software algorithms allow between-centre comparisons. In this study of healthy infants, we quantified MBW measurement errors and tried to improve data quality by simply changing software settings. We analyzed be...
Article
Lung clearance index (LCI) is a marker of ventilation inhomogeneity and is usually elevated in children with cystic fibrosis (CF). However, often LCI values are found normal in infants with CF, although structural lung abnormalities are already detectable. We hypothesized that this discrepancy is due to inadequate algorithms of the available softwa...
Article
Lung clearance index (LCI), a marker of ventilation inhomogeneity, is elevated early in children with cystic fibrosis (CF). However, in infants with CF, LCI values are found to be normal, although structural lung abnormalities are often detectable. We hypothesized that this discrepancy is due to inadequate algorithms of the available software packa...
Article
Full-text available
Asthma is a chronic condition with unknown pathogenesis, and recent evidence suggests that enhanced airway epithelial chloride (Cl-) secretion plays a role in the disease. However, the molecular mechanism underlying Cl- secretion and its relevance in asthma pathophysiology remain unknown. To determine the role of the solute carrier family 26, membe...
Article
Full-text available
Ca(2+)-activated Cl(-) channels (CaCCs) contribute to airway Cl(-) and fluid secretion, and were implicated in the modulation of disease severity and as a therapeutic target in cystic fibrosis (CF). Previous in vitro studies suggested that members of the CLCA gene family, including the murine mCLCA3, contribute to CaCCs. However, the role of mCLCA3...
Article
“Allergic airway inflammation induces a pro-secretory epithelial ion transport phenotype in mice”. P. Anagnostopoulou, L. Dai, J. Schatterny, S. Hirtz, J. Duerr and M.A. Mall. Eur Respir J 2010; 36: 1436–1447. Unfortunately, an error appeared in figure 2 of the above manuscript. The y-axis label of figure 2e) should have been presented as “Amilorid...
Article
Full-text available
The airway epithelium is a central effector tissue in allergic inflammation and T-helper cell (Th) type 2-driven epithelial responses, such as mucus hypersecretion contribute to airflow obstruction in allergic airway disease. Previous in vitro studies demonstrated that Th2 cytokines also act as potent modulators of epithelial ion transport and flui...

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