Pınar Aytar Çelik

• PhD
• Eskişehir Osmangazi University

Halomonas elongata thrives in hypersaline environments producing polyhydroxyalkanoates (PHAs) and osmoprotectants such as ectoine. Despite its biotechnological importance, several aspects of the dynamics of its metabolism remain elusive. Here, we construct and validate a genome‐scale metabolic network model for H. elongata 153B. Then, we investigat...

Prosthetic materials are a source of bacterial infections, with significant morbidity and mortality. Utilizing the bionic “Lotus effect,” we generated superhydrophobic vascular prostheses by nanocoating and investigated their resistance to bacterial colonization. Nanoparticles were generated from silicon dioxide (SiO2), and coated vascular prosthes...

Eighty-seven bacteria isolates were screened for the presence of the levansucrase encoding gene and those possessing the gene were used in gene expression studies and the production of levan. Based on both screening methods, isolate P19-1 was determined as the producer bacterium with the highest amounts of levan. Whole genome sequencing was done to...

In recent years, edible and medicinal mushrooms have a very important position in functional food and bioactive components. Mushrooms attract the attention of the medical industry with their nutritional properties as well as their benefits for consumer health. The objectives of this research was to to reveal the nutritional composition, antioxidant...

In this study, three plasma treatments on biofilm forms of Halomonas caseinilytica (an extremophile organism) i.e., direct and remote non-thermal atmospheric pressure plasma (NTAPP), and plasma-treated water (PTW) were investigated. In contrast to the direct and the remote NTAPP methods, the distilled water (DW) was separately treated with the dire...

Hydrogels in active wound care products are 3D polymeric scaffolds that provide the desired moisture balance in the wound area with their high water retention capacity. Thanks to their biocompatible properties, flexible and porous structures, and high hydrophilic properties, they have frequently been preferred in the repair and regeneration of cell...

Laccase is an important enzyme used in many industries because of its multi-substrate catalyst. New immobilization agents are excellent tools for enhancing the abilities of this enzyme. In this study, immobilization of laccase on silica microparticles with NH2 (S-NH2) surface modification to use in dye removal applications was aimed. The yield of i...

Arsenic penetrates human society through a variety of geological and anthropogenic processes, posing significant health hazards. Acid mine drainage, which contains high concentrations of heavy metals and sulfate, is formed by the biological oxidation of pyrite and other metal-containing sulfidic minerals and is a significant environmental hazard. A...

Yerküredeki su kaynaklarının yaklaşık %97'si okyanuslarda, %2’si buzullardadır; dolayısıyla toplam su rezervinin sadece yaklaşık %0,5-0,8'i tatlı su kaynaklarını kapsamaktadır. Madencilik endüstrisinde cevherlerin flotasyon ile zenginleştirilmesi için yüksek tonajlarda tatlı su kullanılmaktadır. Dahası, madencilik operasyonlarının genellikle tatlı...

In this study, the collecting effect of the biosurfactant obtained from Bacillus subtilis on the flotation of calcite mineral was investigated. NMR and FT-IR analyses show that the obtained biosurfactant is similar to surfactin. It is known that surfactin is a biomolecule used in various industrial fields such as cleaning and cosmetics apart from m...

Bacterial membrane vesicles (BMVs) are known to be critical communication tools in several pathophysiological processes between bacteria and host cells. Given this situation, BMVs for transporting and delivering exogenous therapeutic cargoes have been inspiring as promising platforms for developing smart drug delivery systems (SDDSs). In the first...

Enterococci are commensals of the human intestinal tract. Their use as probiotics is supported by their ability to confer several health benefits and eliminate foodborne pathogens but is controversial due to the presence of virulence and antibiotic resistance traits. To use them as probiotics requires thorough research to establish their safety. He...

For cost-competitive biosynthesis of polyhydroxybutyrate (PHB), the screening of efficient producers and characterization of their genomic potential is fundamental. In this study, 94 newly isolated halophilic strains from Turkish salterns were screened for their polyhydroxyalkanoates (PHAs) biosynthesis capabilities through fermentation. Halomonas...

In carbonate flotation, oleate has been used for many years as a collector in industrial scale. Compared to sulfide minerals, the flotation of carbonate minerals requires more collector consumption and has lower yield and selectivity. It has been previously shown that surfactin provides higher yields in smaller amounts than oleate in the bioflotati...

Species of the Halomonas genus are gram-negative, aerobic, moderately halophilic bacteria that synthesize polyhydroxyalkanoates (PHAs) and other high-value products that have a wide range of potential uses in the food, feed, cosmetics, pharmaceutical, and chemical sectors. Genome sequencing studies allow for the description and comparison of geneti...

Halophilic organisms are a novel attractive option as cell factories for the production of industrially valuable bioproducts. Halomonas elongata is the cell factory of choice for ectoine production, but its levan production has not been well researched. Based on this scientific motivation, in this study, we evaluated the chemical and biological pro...

Comprehension of bacteriology and its physiology and subsequently its application to better the lives of humans have ever been at the core of live science. The development of bacterial strains with optimized desirable functions has been the interest of microbiologists for many years. This has fuelled research interests to understand bacterial physi...

Bacterial membrane vesicles have recently attracted attention as drug delivery systems for antineoplastic agents to target cancerous cells and reduce the side effects of free cytostatic drugs. Due to the complex nature of interactions between the gut microbiota and the host, vesicles released by commensal strains are thought to play a key role in s...

Salt tolerant organisms are increasingly being used for the industrial production of high‐value biomolecules due to their better adaptability compared to mesophiles. Chromohalobacter canadensis is one of the early halophiles to show promising biotechnology potential, which has not been explored to date. Advanced high throughput technologies such as...

In this study, a Plackett-Burman design was applied to investigate critical factors for surface tension. After adding a new factor called “production scale”, a central composite design (CCD) was constructed to examine nonlinear relations among factors and surface tension. An artificial neural network (ANN) was trained using data from CCD experiment...

The interactions of some microorganisms and microbial metabolites with the mineral surface are still being studied thanks to biotechnological advances. Studies on the usability of such microorganisms and metabolites in flotation are gradually increasing due to their potential to be eco-friendlier and more cost-effective alternatives. The primary go...

In this study, a Dielectric Barrier Discharge (DBD)-like non-thermal atmospheric pressure plasma jet (NTAPPJ) was exposed to the Pseudomonas aeruginosa planktonic bacteria. The P. aeruginosa bacteria were treated by the NTAPPJs in both Ar and He at the non-contact distance (contact-free) and the contact distance (direct-contact). The contact of the...

Extremophilic bacteria have numerous uncovered biotechnological potentials. Acidophilic bacteria are important iron oxidizers that are valuable in bioleaching and in studying extreme environments on earth and in space. Despite their obvious potential, little is known about the genetic traits that underpin their metabolic functions, which are equall...

For a long time, agricultural output had been solely dependent on available environmental resources, and increasing pressure on these natural resources to meet the needs of an increasing population continues to disrupt the natural systems of the planet which has led to various consequences. In the past, scientific advancements from the use of manur...

Soil presents a highly heterogeneous medium, and the different components of the soil (sand, silt, clay, and organic matter) enable various habitats for microbial communities which are great potential tools for elucidating community interactions in microbial ecology. These communities are made up of a diversity of organisms from bacteria, archaea,...

Many cells are known to actively release nano-sized outer membrane vesicles (OMVs) that contain bioactive proteins, lipids, and nucleic acids into the extracellular environment. These vesicles have been associated with adaptation to environmental stress in other species, but their role in halophilic salt stress adaptation is not known. This study a...

Pseudomonas aeruginosa biyofilmler kateterler, endeskopik cihazlar ve protezlerde kolonize olurlar. Bu nedenle ısıya hassas plastik endeskopik hortumlar içinde oluşabilen P. aeruginosa biyofilmlerin eradikasyonu önemlidir. Soğuk plazmaların biyolojik hücreler üzerinde ısı üretmeden sterilize edici etkisi klasik yöntemlere göre öne çıkmaktadır. Bunu...

Soğuk plazmalar; biyolojik hücreler ve dokular üzerinde ısı etkisi üretmeden antibakteriyel etkiler oluşturabildiğinden, inaktivasyon ve sterilizasyon amaçları için klasik yöntemlerden farklı yeni bir tekniktir. Soğuk plazmaların ürettiği serbest radikaller (ROS ve RNS), nötr ve yüklü parçacıklar ayrıca UV ışın, ozon ve elektromanyetik alan gibi an...

The depletion of high-grade ore deposits, as well as the generation of large quantities of tailings by mining and metallurgical activities, necessitates an urgent search for not only more cost-effective, but also more eco-friendly methods of recovering minerals from low-grade deposits and secondary resources. Research in the bioflotation process ap...

As environmental concerns grow, flotation researches, particularly for the recovery of fine-grained ores, encourage "bioflotation" studies, in which biological origin alternatives are used instead of traditional flotation reactives. While bioflotation applications starting from the pyrite mineral have spread to many carbonate and oxide minerals ove...

Bacterial membrane vesicles are cupped-shaped structures formed by bacteria in response to environmental stress, genetic alteration, antibiotic exposure, and others. Due to the structural similarities shared with the producer organism, they can retain certain characteristics like stimulating immune responses. They are also able to carry molecules f...

Changes in the environment in recent years have led to the examination of the role and influence human activities on it. Attempts to quantify the impact of these activities by developing standardized modelling frameworks through Life cycle sustainability assessment is one such approach in the context of sustainability seeking to measure the impact...

Since it can be a source of new microorganisms with biological potential, it is important to identify the microorganisms found in environments with high boron content in terms of ecological and biotechnological application potentials. In this context, deposit waters in environments where boron mining activities perform are important habitats for bo...

Mucor circinelloides isolated from mill scale was magnetized by using the method of co-precipitation under alkaline conditions and this magnetized fungus was utilized efficiently to remove reactive textile dyestuff such as Reactive Orange 13. The value of saturation magnetization was obtained 4.93083 emu/g and Brunauer-Emmett-Teller surface area fo...

Acid mine drainage (AMD), generated in the active and abandoned mine sites, is characterized by low pH and high metal concentrations. One AMD treatment possibility is biologically oxidizing Fe²⁺ followed by precipitation through pH control. As compared to autotrophic iron oxidizing microbial community, a microbial community enriched in the presence...

Acid mine drainage (AMD) is a wastewater caused by mining activities and sulfide minerals oxidation. It occurs generally as a result of the relation of water, oxygen and microorganisms. In this study, the pH and the temperature of samples were determined around 2 and 20 °C, respectively. The chemical analysis was made by - ICP-MS. Sulfur concentrat...

During 2020, the world has experienced extreme vulnerability in the face of a disease outbreak. The coronavirus disease 2019 (COVID-19) pandemic discovered in China and rapidly spread across the globe, infecting millions, causing hundreds of thousands of deaths, and severe downturns in the economies of countries worldwide. Biosurfactants can play a...

This study aims at investigating the iron oxidation and filtration performances of a ceramic membrane bioreactor (CMBR) inoculated with a mixed culture of iron-oxidizing acidophilic bacteria isolated from a real acid mine drainage (AMD). The CMBR was fed with a synthetic solution containing 1400 mg/L Fe²⁺ at pH 1.5−2.5. High iron oxidation performa...

Thermophilic microorganisms are quite attractive for the study of biodiversity and evolutionary process as well as biotechnological applications. These organisms provide significant advantages for industrial and biotechnological processes occurring fast and efficiently at high temperatures. Possible potential also is getting increase thanks to isol...

12 halophilic strains from different hypersaline environments such as solar salterns in Tuzlagozu (Sivas), Fadlum (Sivas), Kemah (Erzincan), a hypersaline spring water in Pulumur (Tunceli) and a saline lake in Delice (Kirikkale) belonging to Turkey, were investigated in terms of levan production. After incubation and ethyl alcohol treatment, dialys...

Microbial diversity, same as mineral deposits has been evaluated to be among the underground riches of a country. New data on microbial diversity are thought to be of potential economic value. Acid drainage waters are characterized by low pH and high concentrations of metals. Therefore, these acidic habitats are perfect places for quantitative and...

In this study, production, optimization and characterization of protease produced by a bacterium isolated from Kırka boron mine distinct were performed. The proteolytic activities of isolates were screened based on Anson method. The optimal production conditions for protease were determined by using Central Composite Design. Bacillus sp. DB14 isola...

Acid mine drainages (AMDs) are characterized as low pH water containing metal, which is the best-known habitat for the microorganisms described as acidophiles. Most of these members do not survive above pH 4.0; therefore they are acidophilic. Because of these metabolic properties of acidophilic microorganisms, AMD is accepted as a model system in b...

BACKGROUND In this study, hyaluronic acid (HA) was produced using Streptococcus equi ssp. equi via an optimized Central Composite Design process, with purification by an alternative extraction method. Determinations of molecular weight, cytotoxicity, viscosity; proton nuclear magnetic resonance (H‐NMR) and Fourier transform infrared‐attenuated tota...

The polymers/enzymes produced by the microorganisms isolated from the various thermal sources have stability at different levels and their usage areas can be improved according to this characteristic. In this study, prokaryotic diversity of water sample taken from Çitgöl thermal spring in Kütahya-Simav, has been investigated by cultivation techniqu...

Coal continues to be a significant source of energy in the world. It is very important to utilize this energy source as much as possible, to operate unutilized loss reserves due to its characteristics. In this context, the necessity to continue studying on clean coal technologies was emphasized in terms of sustainability in energy production and it...

Boron is a vital micronutrient for the growth of plants and different organisms. However, boron may be toxic to living cells at high concentrations. Nevertheless, microorganisms can be grown in harsh conditions such as environments with higher boron concentrations. It is also known that boron‐tolerant microorganisms have significant biotechnologica...

Biological desulphurization is a good option because of simple equipment, easy operation, and low cost advantages. In this study, biodesulphurization of low rank lignites having high ash and sulphur content was investigated through a drum reactor using Alternaria sp. fungus which provided high removal of sulphur at erlen scale in our previous study...

Hyaluronic acid (HA) is a natural biopolymer and has long been attracting the attention of biotechnology industry due to its various biological functions. HA production with natural producer Streptococcus equi subsp. zooepidemicus has not been preferred because it has many drawbacks due to its pathogenicity. Therefore, in the present study, Strepto...

In the present study, culture conditions of Streptococcus equi was optimized through Box–Behnken experimental design for hyaluronic acid production. About 0.87 gL⁻¹ of hyaluronic acid was produced under the determined conditions and optimal conditions were found as 38.42 °C, 24 hr and 250 rpm. The validity and practicability of this statistical opt...

This study was aimed to assess toxicity of boron mine drainage (BMD) water in Kırka borough of Turkey, which is one of the most important boron deposit sites in the world. Three complementary tests were done to assess the toxicity of BMD water. Root growth inhibition tests showed that EC50 value of BMD water sample was approximately 3.85% of concen...

Rhamnolipid, among the most effective biosurfactants, is a glycolipid‐type biosurfactant primarily produced by Pseudomonas aeruginosa. In this study, rhamnolipid production was carried out using a strain of P. aeruginosa and it is aimed to compare rhamnolipid biopolymers obtained from various extraction methods using glycine (RG), hydrochloric acid...

In this study, the alkaline protease-production capacity of a Geobacillus subterraneus C2-1 isolate from the Çitgöl thermal spring was investigated and optimised using a Plackett-Burman experimental design. In addition to the incubation time, which was the most important parameter, other significant factors were the peptone, glucose, and MgSO4.7H2O...

Biosorption potential of Talaromayces aculeatus AMDC-14 (KF588645) as low-cost biosorbent for different dyes (Reactive Blue 13, Reactive Blue 72, Reactive Yellow 85 and Reactive Orange 13) removal from aqueous medium was studied. Experiments were performed to determine effect of initial solution pH (1-7), biosorbent dose (0.1-2.5 g L-1), time (15-1...

In this study, first, the physicochemical cleaning of Cayirhan region’s low-quality lignites was investigated by flotation to reduce ash content. In the flotation experiments, the effects of collector dosage, frother dosage, dispersant dosage, pulp density and air flow rate on ash content and combustible recovery in the concentrate fraction were op...

Nanotechnology involves the study and use of materials under the 100 nm scale, exploiting the different physiochemical properties exhibited by these materials at the nanoscale level. Microorganisms are the best model and role of action for the nano/biotechnological applications. This technology has become increasingly important for the biotechnolog...

Objective: Biosurfactants are surface-active substances produced by microorganisms. The growth of microorganism supports biosurfactant production on hydrocarbon polluted environments. In this study, the capacity of biosurfactant production of Georgenia daeguensis (accession number, KP798810) isolated from hydrocarbon- contaminated soil was studied....

In this study, acidic textile dyes, including Reactive Blue 13 and Reactive Blue 72, were adsorbed by Penicillium ochrochloron AMDB-12 isolated from acidic mine drainage. Optimal conditions in terms of pH, initial dye concentration, agitation rate, biomass dosage, contact time, and temperature for this process were determined. Biosorption yields fo...

This study concentrates on the reactive biosorption of dyes such as Reactive Red 3:1 (RR3:1), Reactive Orange 13 (RO13), Reactive Blue 13 (RB13), Reactive Blue 72 (RB72). The modified fungal biosorbent, Paecilomyces sp., had a higher dye biosorption yield in our study. 100% of biosorption yield observed on RR3:1 and RB13 and 94.09% of color removal...

The biodiversity of acidophilic prokaryotes in two acidic (pH 2.8-3.05) mine drainage (AMD) sites (Balya and Çan) in Turkey was examined using a combined cultivation-based and cultivation-independent approach. The latter included analyzing microbial diversity using fluorescent in situ hybridization (FISH), terminal restriction enzyme fragment lengt...

The Pb(II) and Ni(II) biosorption of a fungal biomass isolated from mine drainage of metal-processing industries in Balya (BalA +/- kesir province, Turkey) was optimized using a response surface methodology by altering parameters such as pH, initial metal concentration, contact time and biosorbent dosage. This strain was shown to be highly similar...

Magnetically modified Trametes versicolor cells were used for biosorption of Reactive Blue 13 (RB13), Reactive Yellow 85 (RY85) and Reactive Violet 1 (RV1). Percent biosorption values and maximum adsorption capacities of 98.30% and 135.35 mg g−1 for RB13, 96.02% and 125 mg g−1 for RY85, and 98.56% and 227.27 mg g−1 for RV1 were observed under optim...

Objective: The aim of this study was to investigate the laccase production of Trametes versicolor under submerged fermentation condition. Then, dye decolorization by laccase was optimized using Box-Behnken methodology. Methods: The optimal culture conditions for producing high amount of laccase were determined using Taguchi methodology. The experim...

The biodesulfurization capability of a strain having sulfur and iron metabolism isolated from acidic mine drainage of Balya (Balikesir, Turkey) was studied. Molecular identification of the 16S rRNA gene showed that this bacterium was a strain of Acidithiobacillus ferrivorans. Desulfurization optimization experiments were performed by Taguchi’s meth...

Abstract In this study, protease-producing capacity of Bacillus pumilus D3, isolated from hydrocarbon contaminated soil, was evaluated and optimized. Optimum growing conditions for B. pumilus D3 in terms of protease production were determined as 1% optimum inoculum size, 35 °C temperature, 11 pH and 48 h incubation time, respectively. Stability stu...

Removal of textile dyestuffs from aqueous solution by biosorption onto a dead fungal biomass isolated from acidic mine drainage in the Çanakkale Region of Turkey was investigated. The fungus was found to be a promising biosorbent and identified as Paecilomyces sp. The optimal conditions for bioremediation were as follows: pH, 2.0; initial dyestuff...

Olive oil mill wastewater (OMWW), a recalcitrant pollutant, has features including high phenolic content and dark color; thereby, several chemical or physical treatments or biological processes were not able to remediate it. In this study, the treatment efficiencies of three treatments, including adsorption, biological application, and photo-Fenton...

Chlorinated compounds constitute an important class of xenobiotics. Crude laccase was produced using Trametes versicolor ATCC (200801) in potato dextrose broth, with wheat bran as an inducing medium, and its ability to dechlorinate eight compounds was determined. The compounds were 2-chlorophenol, 4-chlorophenol, 2,4-dichlorophenol, 2,6-dichlorophe...

This study presents Saccharomyces cerevisiae, an alcohol fermentation waste, for removal of acidic dyes (Red 3:1 & Orange 13) even after chemical and physical modification. Biosorption performance was found 96.29% through untreated biomass and 97.31% through pretreated biomass with hydrogen peroxide solution for Red 3:1, whereas 93.49% through untr...

In this study, decolorization of dyestuffs, such as Reactive Red 198, Rem Blue RR, Dylon Navy 17, Rem Red RR, and Rem Yellow RR was studied using laccase and laccase-mediated system. The laccases are known to have an important potential for remediation of pollutants. Among these dyestuffs, decolorization of Rem Blue RR and Dylon Navy 17 was perform...

Large quantities of dyes used in the textile industry are discharged to recipient environment during manufacture. This situation is beginning of a process which is difficult to recovery and relevant toenvironment and human health. Therefore, pollution of dyestuff produced textile industry will be reduced by cleaning of polluted area and integrating...

In this paper, data obtained during the oxidative desulphurization of some low-rank Turkish lignites with crude laccase enzyme produced from Trametes versicolor ATCC 200801 are presented. In order to optimize desulphurization conditions, effects of incubation time, pulp density, incubation temperature, medium pH, and also lignite source on the desu...

The aim of this study is to investigate benefit and cost analysis of enzyme production with solid state and submerged fermentation techniques, biotechnological processes within production of value-added products. The approach of benefit and cost analysis is commonly used a way at deciding and investigating of the project. This analysis was referred...

Lacasse is one of the extracellular enzymes excreted from white and brown rot fungi, which is involved in ligninolysis. In the present study, the effects of the addition of lacasse inducers to the medium on enhancement of enzyme production under conditions of submerged fermentation were researched. At first, a culture medium was selected suitable f...

Cyanide, a hazardous substance, is released into the environment as a result of natural processes of various industrial activities which is a toxic pollutant according to Environmental Protection Agency. In nature, some microorganisms are responsible for the degradation of cyanide, but there is only limited information about the degradation charact...

The potentials of thirteen lignocellulosic wastes wheat bran, barley bran, cone of Pinus nigra, sawdust, corn bran, oat, rice bran, canola, dried tea, ground clover straw, sunflower stalk, soybean bagasse, dried distillers grains with soluble (DDGS) as solid substrates in respect of laccase production by the white-rot fungi Trametes versicolor (ATC...

Biodesulphurization experiments were carried out with Tunçbilek lignite, characterized by high sulfur content (2.59%) by using Trametes versicolor ATCC 200801 and Phanerochaete chrysosporium ME 446. At fungal biomass studies, the effects of various parameters on fungal desulphurization of coals such as pH, temperature, pulp density, incubation time...