Pilar Peral-García

Pilar Peral-García
National Scientific and Technical Research Council | conicet · CCT La Plata

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84
Publications
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1,266
Citations
Citations since 2017
5 Research Items
610 Citations
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2017201820192020202120222023020406080100
2017201820192020202120222023020406080100
2017201820192020202120222023020406080100

Publications

Publications (84)
Article
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Debido al Aislamiento Social, Obligatorio y Preventivo (ASPO) por el COVID-19, la Universidad Nacional de La Plata comunicó a sus docentes la necesidad de mantener la continuidad pedagógica en todas sus dependencias. En la Facultad de Ciencias Veterinarias las TIC´S se comenzaron a utilizar ampliamente en los últimos años para mejorar los modelos f...
Article
Cattle are a monotocous species, despite naturally conceived multiple births are sometimes observed. Although the number of twins has consistently increased, triplet and quadruplet pregnancies represent 0.015 and 0.004% of the total births, respectively. Multiple births are the result of multiple ovulation and/or the spontaneous cleavage of one fer...
Article
Copy number variation (CNV) has been proved to be widespread in human, animal and plant genomes. Together with single nucleotide polymorphisms (SNPs), CVNs play a key role in genetic diversity. In this study, genome-wide detection of CNVs was performed based on SNP data from 24 Criollo Argentino horses genotyped with the GGP Equine70k array. Overal...
Article
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The study was conducted in a dairy establishment located in the Province of Buenos Aires. The parameters of hygienic, sanitary and compositional quality of raw milk were evaluated from biweekly reports, made by the dairy. Personnel, facilities and rodeo management were monitored to detect the factors responsible for poor milk quality. Subsequently...
Article
Equine lentivirus receptor-1 (ELR1) has been characterized as the specific functional receptor that mediates equine infectious anemia virus (EIAV) entrance to horse macrophages. This receptor is tumor necrosis factor receptor superfamily member 14 (TNFRSF14). The aim of this study was to investigate the occurrence of allelic variants in the coding...
Article
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Non-genotoxic carcinogens are substances that induce tumorigenesis by non-mutagenic mechanisms and long term rodent bioassays are required to identify them. Recent studies have shown that transcription profiling can be applied to develop early identifiers for long term phenotypes. In this study, we used rat liver expression profiles from the NTP (N...
Article
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Foodborne pathogens can cause acute and chronic diseases and produce a wide range of symptoms. Since the consumption of ground beef is a risk factor for infections with some bacterial pathogens, we performed a comprehensive evaluation of butcher shops, implemented improvement actions for both butcher shops and consumers, and verified the impact of...
Article
Escherichia coli O157:H7 is an enteric pathogen associated with food safety threats and with significant morbidity and mortality worldwide. In Argentina, post-enteric hemolytic uremic syndrome (HUS) is endemic, with >70% of cases associated with E. coli O157 infection. To date the biological basis behind the severity among E. coli O157 infections i...
Article
Breed assignment has proved to be useful to control meat trade and protect the value of special productions. Meat-related frauds have been detected in China; therefore, 95 SNPs selected from the ISAG core panel were evaluated to develop an automated and technologically updated tool to screen breed label fraud in the Chinese meat market. A total of...
Article
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Age at puberty is an important component of reproductive performance in cattle, so it is important to identify genes that contribute to the regulation of the onset of puberty and polymorphisms that explain differences between bulls. In a previous study, we found putative associations between age at puberty in Angus bulls and single-nucleotide polym...
Article
In bovine, there are significant differences among and within breeds in the time when bulls reach puberty. Reported data indicate that age at puberty has a strong genetic component and is a multigenic trait. However, despite significant advancements in the field, the gene network controlling puberty is very complex, containing a host of genes that...
Article
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The genetic diversity and structure of 72 Appaloosa horses belonging to a closed breeding population from an ecological reserve in Buenos Aires, Argentina, was investigated using eight microsatellite markers from the International Society for Animal Genetics panel. Our data showed that this Appaloosa horse population had an elevated degree of genet...
Article
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Puberty is a stage of sexual development determined by the interaction of many loci and environmental factors. Identification of genes contributing to genetic variation in this character can assist with selection for early pubertal bulls, improving genetic progress in livestock breeding. Thyroid hormones play an important role in sexual development...
Article
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ContentsThe aim of this study was to investigate the influence of zinc (Zn) on the health of cumulus–oocyte complex (COC) during in vitro maturation (IVM). Experiments were designed to evaluate the effect of Zn added to IVM medium on: DNA integrity, apoptosis, cumulus expansion and superoxide dismutase (SOD) activity of cumulus cells (CC). Also, ro...
Article
Short tandem repeats (STR)s have been the eligible markers for forensic animal genetics, despite single-nucleotide polymorphisms (SNP)s became acceptable. The technology, the type, and amount of markers could limit the investigation in degraded forensic samples. The performance of a 32-SNP panel genotyped through OpenArraysTM (real-time PCR based)...
Article
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During the last decade, microsatellites (short tandem repeats or STRs) have been successfully used for animal genetic identification, traceability and paternity, although in recent year single nucleotide polymorphisms (SNPs) have been increasingly used for this purpose. An efficient SNP identification system requires a marker set with enough power...
Article
The aim of this study was to investigate if elemental and isotopic signatures of Argentinean wheat can be used to develop a reliable fingerprint to assess its geographical provenance. For this pilot study we used wheat cultivated at three different regions (Buenos Aires, Córdoba and Entre Ríos), together with matching soil and water. Elemental comp...
Article
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The value of the traceability and labeling of food is attributable to two main aspects: health safety and/or product or process certification. The identification of the species related to meat production is an important matter for economic, religious and health reasons. Many approaches and technologies have been used for species identification in a...
Article
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Polymorphisms at Major Histocompatibility Complex (MHC) genes have been associated with resistance/susceptibility to infectious diseases in domestic animals. The aim of this investigation was to evaluate whether polymorphisms of the DRA gene the Equine Lymphocyte Antigen is associated with susceptibility to Equine Arteritis Virus (EAV) infection in...
Article
Full-text available
In bovines, there are significant differences within and among beef breeds in the time when bulls reach puberty. Although the timing of puberty is likely to be a multigenic trait, previous studies indicate that there may also be single genes that exert major effects on the timing of puberty within the general population. Despite its economic import...
Data
Table S1. Gene frequency, Hardy-Weinberg equilibrium (HWE), expected heterozygosities (he), and gene differentiation estimated for GNRHR-SNP5, GNRHR-SNP6, LHR-I499L and IGF1-SnaBI alleles identified by pyrosequencing analysis within each herd and in the whole sample. Table S2. Average estimated puberty age and weigh estimated at 300 days (W 300), w...
Article
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Shiga toxin-producing Escherichia coli (STEC) are foodborne pathogens that cause mild or serious diseases and can lead to people death. This study reports the prevalence and characteristics of STEC O157 and non-O157 in commercial ground beef and environmental samples, including meat table, knife, meat mincing machine, and manipulator hands (n = 450...
Article
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Cattle was absent from America before the discovery. Initially, bovine were brought to Greater Antilles (La Española, Puerto Rico, Jamaica and Cuba islands), and in the course of a few years, they were taken from Caribbean islands to the rest of Latin America. Nowadays, Cuban Creole cattle population is about 1300 heads, mainly located in the easte...
Article
The present research was undertaken in order to study the effect of repeated low doses of radiation on two different cell lines from the same species. An in vitro model test was developed to avoid the influence of the confounding factors affecting epidemiological studies and to simulate a chronic exposure (50 mSv of x-rays during ten consecutive da...
Article
Buffaloes (Bubalus bubalis) are widely distributed and were introduced to Brazil in 1895. Most of the molecular genetic characterization of buffaloes has been done with cross-specific (cattle) markers, but few of them include Brazilian populations. Nineteen commonly used cattle microsatellites were tested to develop a multiplexed set of microsatell...
Article
The main goal of this study was to propose a reliable method to verify the geographical origin of meat, establishing the influence of soil and water on its isotopic and elemental composition. Thus, beef meat, soil, and water samples were collected from three major cattle-producing regions of Argentina (Buenos Aires, Córdoba, and Entre Ríos). Multie...
Article
We investigated the association of equine arteritis virus (EAV) infection and three short tandem repeat (STR) polymorphisms located within or in close proximity to equine lymphocyte antigen (ELA) region. We used a case-control design as a first approach before proceeding to select candidate genes. One hundred and sixty-five Silla Argentino horses w...
Article
Our main goal was to investigate if robust chemical fingerprints could be developed for three Argentinean red wines based on organic, inorganic, and isotopic patterns, in relation to the regional soil composition. Soils and wines from three regions (Mendoza, San Juan, and Córdoba) and three varieties (Cabernet Sauvignon, Malbec, and Syrah) were col...
Article
Many candidate genes have been suggested as responsible for marbling in beef cattle, for instance diacylglycerol O-acyltransferase 1, thyroglobulin, growth hormone, leptin and stearoyl CoA desaturase. The objective of the present work was to evaluate the polymorphisms of five SNPs of these candidate genes in 389 animals of 18 Bos Taurus and Bos ind...
Article
Genetic markers have been used to assess the association of economically important traits with cattle under intensive feeding conditions; however, there is still the need to ascertain the usefulness of these markers under extensive production systems. Bovine chromosome 5 has been widely studied because several QTLs have been detected. Microsatellit...
Article
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American Creole bovines and equines are direct descendant from the animals introduced by European conquerors during XV an XVI centuries. At the beginning of the last decade, new technologies based in DNA emerged for genetic analysis, and Creole breeds were not the exception for these kind of studies. During the last years, these methodologies evolv...
Article
Exposure to environmental, diagnostic, and occupational sources of radiation frequently involves low doses. Although these doses have no immediately noticeable impact on human health there is great interest in their long-term biological effects. To assess immediate and time-delayed DNA damage in two cell lines exposed to low doses of ionizing radia...
Article
The purpose of this study was to investigate the effect of Zn (zinc) concentration on CCs (cumulus cells) during in vitro maturation. For this purpose, DNA integrity of CCs by addition of different Zn concentrations [0 (control); 0.7 μg/ml (Zn1); 1.1 μg/ml (Zn2) and 1.5 μg/ml (Zn3)] to the culture medium was evaluated by comet assay. In addition, e...
Article
Gonadotropin releasing hormone and its receptor (GNRHR) play a critical role in sexual differentiation and reproduction. Available evidence shows a strong genetic component in the timing of puberty. In bovines, there are significant differences within and among beef breeds in the time when bulls reach puberty. Despite its economic importance, there...
Article
Bovine chromosome 5 has been widely studied because several QTLs have been detected there, in particular for growth and fat traits. Even though most of the beef is produced under pasture based conditions, only little research has focused on this kind of systems. Two QTL regions, neighboring the Myogenic factor 5 gene, and Insulin-like Growth Factor...
Article
Full-text available
Buffaloes (Bubalus bubalis) were introduced to Brazil in 1895. Many authors have used microsatellites for genetic characterization of buffalo, but only some papers include molecular markers in Brazilian populations. Cross-species utility of microsatellites is one of the preferred marker choice criteria. To develop a multiplexed set of microsatellit...
Article
Glutathione (GSH) concentration increases in bovine oocytes during in vitro maturation (IVM). The constitutive amino acids involved in GSH synthesis are glycine (Gly), glutamate (Glu) and cysteine (Cys). The present study was conducted to investigate the effect of the availability of glucose, Cys, Gly and Glu on GSH synthesis during IVM. The effect...
Article
The polymorphism of equine lymphocyte antigen (ELA) class II DRA gene had been detected by polymerase chain reaction-single-strand conformational polymorphism (PCR-SSCP) and reference strand-mediated conformation analysis. These methodologies allowed to identify 11 ELA-DRA exon 2 sequences, three of which are widely distributed among domestic horse...
Article
In order to detect switching and/or manipulation of samples, the owner of a stallion asked our lab to perform a DNA test on a positive doping urine sample. The objective was to compare the urine DNA profile versus blood and hair DNA profiles from the same stallion. At first, 10 microsatellite markers were investigated to determine the horse identit...
Article
Full-text available
Genetic identification has been continuously evolving during the last century. The recent development of whole-genome projects allowed the discovery and characterization of a large number of single nucleotide polymorphisms (SNP). A number of high-throughput DNA methods has decreased the cost of DNA marker analysis and increased the amount of sample...
Article
Estimation of population subdivision using genetic markers shows that genetic differentiation in livestock and pet breeds is significantly higher than in human populations. Nevertheless, the influence of population substructure and sample size on match probability has not been extensively analyzed in domestic species. To evaluate the magnitude of t...
Article
Recently, the use of DNA markers has provided a more accurate method of identifying individuals and verifying parentage. In this report, we describe foal assignment in a farm bred jumping horses (Silla argentino). Ten mares were freely served by two stallions, resulting in nine foals. Weaning occurred without registration of the mare of each offspr...
Article
In order to clarify the historical origin and phylogeographic affinities of Creole cattle matrilineages throughout the American continent, we analysed published D-loop mtDNA sequences (n = 454) from Creole, Iberian and African cattle breeds. The Western European T3 haplogroup was the most common in American Creole cattle (63.6%), followed by the Af...
Article
Two new alleles, named BoLA-DRB3-P*06 and BoLA-DRB3-P*07, have been identified for the upstream regulatory region of the BoLA-DRB3 gene. The 228-bp nucleotide sequences of the promoter comprising the W, X, Y, CAAT and TATA regulatory boxes were analysed. The BoLA-DRB3-P*06 exhibits one insertion between the W and X boxes, and one transition between...
Article
Sequence information was obtained on the variation of the ELA-DRB upstream regulatory region (URR) after polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) cloning and sequencing of approximately 220 bp upstream of the first exon of horse DRB genes. The sequence of the proximal URR of equine DRB is composed of highly conse...
Article
We developed a simple, quick assay in order to discriminate forensic samples among human, and common domestic and livestock species of the Pampean region, Argentina. A mitochondrial cytochrome b fragment amplified with universal primers was separately digested with three restriction enzymes (AluI, HaeIII, and HinfI) and the resulting fragments were...
Article
In the present work, we describe through polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing the polymorphism within the URR-BoLA-DRB3 in 15 cattle breeds. In total, seven PCR-SSCP defined alleles were detected. The alignment of studied sequences showed six polymorphic sites (four transitions, one transve...
Article
DNA profiling was used as evidence to assign paternity in a dispute between two neighbors in a judicial case of undue appropriation of cattle offspring from five alleged Holstein sires. Five offspring were genotyped using ten genetic markers (nine microsatellites and the BOLA-DRB3 locus). The computer program CERVUS was used to estimate the LOD sco...
Article
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The typing method based on PCR-RFLP for the BoLA-DRB3 exon 2 was extensively used in populational and disease association studies. To date, more than fifty PCR-RFLP variants were described in domestic cattle. Six BoLA-DRB3 variants correspond to alleles that have not been sequenced yet (DRB3.2*4, 14, 17,25,39 and 40). GenomicDNA was purified froma...
Article
The second exon of equine leucocyte antigen (ELA)-DQB genes was amplified from genomic DNA of 32 Argentine Creole horses by PCR. Amplified DNA was analysed by PCR-restriction fragment length polymorphism (RFLP) and PCR-single-strand conformation polymorphism (SSCP). The PCR-RFLP analysis revealed two HaeIII patterns, four RsaI patterns, five MspI p...
Article
Summary The sequence of the proximal upstream regulatory region (URR) of the bovine DRB genes was amplified using oligonucleotide primers designed from the consensus among DRB sequences from different species. The obtained DNA sequence was 234 bp long and composed of highly conserved sequence motifs, showing the same organization as the HLA-DRB, H2...
Article
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Penile hypoplasia is a rare congenital disorder that has been described in a few breeds of dogs. The aim of this article was to report a case of penile hypoplasia in an inbred Rottweiler dog. An otherwise healthy, 3 years old Rottweiler male dog, was referred to our Faculty because of inability to copulate. The male had a history of normal libido....
Article
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Data from five protein-coding loci related to dairy production were used to study the genetic diversity and population structure of Argentine and Bolivian Creole cattle breeds. Genomic DNA was extracted from blood samples of six Creole cattle breeds: Argentine (n = 230), Patagonian (n = 25); "Saavedreño" (n = 140), "Chaqueño Boliviano" (n = 30), "Y...
Article
South American horses constitute a direct remnant of the Iberian horses brought to the New World by the Spanish conquerors. The source of the original horses was Spain, and it is generally assumed that the animals belonged to the Andalusian, Spanish Celtic, Barb or Arabian breeds. In order to establish the relationship between Argentinean and Spani...
Article
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A fragment of 466 base pairs from a highly variable peripheral region of the mitochondrial D-loop of horses was amplified and analyzed by single stranded conformational polymorphism (SSCP). Fourteen distinct SSCP variants were detected in 100 horses belonging to four breeds (Arabian, ARB; Thoroughbred, TB; Argentinian Creole, ARC; and Peruvian Paso...
Article
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Genetic polymorphism was analyzed for five blood proteins: albumin - Al, esterase - Es, alpha(1)B-glycoprotein - Xk, transferrin - Tf and hemoglobin - Hb in 200 Thoroughbred (TB) and 124 Argentine Creole (AC) horses. Of the five systems examined, Tf and Hb were not in Hardy-Weinberg equilibrium in either breed and Es was not in equilibrium in the C...
Article
DNA profiling was used as probative evidence in a cattle stealing case. The carcasses of the dead animals were found from a report and a farmer recognized the remains as those corresponding to the stolen animals by the farm mark on the coat. Those remains were collected as reference samples. Meat pieces were sequestered from a butchery and then sen...
Article
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Contrary to highly selected commercial breeds, indigenous domestic breeds are composed of semi-wild or feral populations subjected to reduced levels of artificial selection. As a consequence, many of these breeds have become locally adapted to a wide range of environments, showing high levels of phenotypic variability and increased fitness under na...
Article
Genetic variation in the equine leucocyte antigen-DRB (ELA-DRB) second exon was investigated using polymerase chain reaction (PCR) amplification, restriction fragment length polymorphism (RFLP) of PCR products (PCR-RFLP) and deoxyribonucleic acid (DNA) sequencing. Eight distinct PCR-RFLP patterns could be identified in the studied Argentine Creole...
Article
Full-text available
Most class II genes show a wide genetic variation both in the number of loci and the number of alleles among and within species. Sequence conservation during evolution has proven to be high enough in domestic animals for primer sequences developed for one species to be employed to amplify sequences of another. The aim of this work was to develop a...
Article
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RESUMEN: La gonadotrofina corionica equina (eGC) es una hormona glicoproteica producida por las células del trofoblasto fetal que invaden el endometrio uterino. La elevada actividad de esta hormona en la estimulación del desarrollo folicular y su alta persistencia in vivo permite que sea usada como agente estimulante en especies heterólogas. El obj...

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