Pilar Garcia Guerreiro

Pilar Garcia Guerreiro
Autonomous University of Barcelona | UAB · Department of Genetics and Microbiology

PhD

About

66
Publications
4,106
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468
Citations

Publications

Publications (66)
Article
Full-text available
Interspecific hybridization is often seen as a genomic stress that may lead to new gene expression patterns and deregulation of transposable elements (TEs). The understanding of expression changes in hybrids compared to parental species is essential to disentangle their putative role in speciation processes. However, to date we ignore the detailed...
Article
Full-text available
Drosophila melanogaster is a leading model in population genetics and genomics, and a growing number of whole-genome data sets from natural populations of this species have been published over the last years. A major challenge is the integration of disparate data sets, often generated using different sequencing technologies and bioinformatic pipeli...
Article
Full-text available
Drosophila melanogaster is a leading model in population genetics and genomics, and a growing number of whole-genome datasets from natural populations of this species have been published over the last years. A major challenge is the integration of disparate datasets, often generated using different sequencing technologies and bioinformatic pipeline...
Preprint
Full-text available
Drosophila melanogaster is a leading model in population genetics and genomics, and a growing number of whole-genome datasets from natural populations of this species have been published over the last 20 years. A major challenge is the integration of these disparate datasets, often generated using different sequencing technologies and bioinformatic...
Article
Full-text available
Genetic variation is the fuel of evolution, with standing genetic variation especially important for short-term evolution and local adaptation. To date, studies of spatio-temporal patterns of genetic variation in natural populations have been challenging, as comprehensive sampling is logistically difficult, and sequencing of entire populations cost...
Article
Full-text available
Almost all eukaryotes have transposable elements (TEs) against which they have developed defense mechanisms. In the Drosophila germline, the main transposable element (TE) regulation pathway is mediated by specific Piwi-interacting small RNAs (piRNAs). Nonetheless, for unknown reasons, TEs sometimes escape cellular control during interspecific hybr...
Article
Full-text available
Background: Drosophila subobscura exhibits a rich inversion polymorphism, with some adaptive inversions showing repeatable spatiotemporal patterns in frequencies related to temperature. Previous studies reported increased basal HSP70 protein levels in homokaryotypic strains for a warm-climate arrangement compared to a cold-climate one. These findi...
Article
Full-text available
Heat-shock (HS) assays to understand the connection between standing inversion variation and evolutionary response to climate change in D. subobscura found that “warm-climate” inversion O3+4 exhibits non-HS levels of Hsp70 protein like those of “cold-climate” OST after HS induction. This was unexpected, as overexpression of Hsp70 can incur multiple...
Article
Full-text available
All genomes contain repeated sequences that are known as transposable elements (TEs). Among these are endogenous retroviruses (ERVs), which are sequences similar to retroviruses and are transmitted across generations from parent to progeny. These sequences are controlled in genomes through epigenetic mechanisms. At the center of the epigenetic cont...
Thesis
Full-text available
Numerous studies have confirmed the adaptive value of the rich chromosomal inversion polymorphism in the drosophilid D. subobscura. However, until recently very little was known about the molecular basis behind its maintenance in natural populations. In search of candidate loci, a previous heat shock experiment quantified Hsp70 protein levels in ho...
Preprint
Full-text available
Genetic variation is the fuel of evolution. However, analyzing dynamics of evolutionary change in natural populations is challenging, genome sequencing of entire populations remains costly and comprehensive sample collection logistically challenging. To tackle this issue and to define relevant spatial and temporal scales of variation for a populati...
Article
Full-text available
Interspecific hybridization is a genomic stress condition that leads to the activation of transposable elements (TEs) in both animals and plants. In hybrids between Drosophila buzzatii and Drosophila koepferae, mobilization of at least 28 TEs has been described. However, the molecular mechanisms underlying this TE release remain poorly understood....
Article
Full-text available
Genome size (or C-value) can present a wide range of values among eukaryotes. This variation has been attributed to differences in the amplification and deletion of different non-coding repetitive sequences, particularly transposable elements (TEs). TEs can be activated under different stress conditions such as interspecific hybridization events, a...
Article
Full-text available
Transposable elements (TEs), repeated mobile sequences, are ubiquitous in the eukaryotic kingdom. Their mobilizing capacity confers on them a high mutagenic potential, which must be strongly regulated to guarantee genome stability. In the Drosophila germline, a small RNA-mediated silencing system, the piRNA (Piwi-interacting RNA) pathway, is the ma...
Data
FISH of Helena RNA expression in different BC1 hybrid testes. Red staining are Helena transcripts, green staining is tissue autofluorescence. Arrows mark the presence of Helena transcripts. (TIFF)
Data
FISH of Helena RNA expression in different BC2 hybrid testes. Red staining are Helena transcripts, green staining is tissue autofluorescence. Arrows mark the presence of Helena transcripts. (TIFF)
Data
Southern blot analysis of Helena in parental species, D. buzzatii (left) and D. koepferae (right). No restriction sites for AatII are present in Helena’s probe sequence. Thus, digestions with this enzyme allow us to distinguish different Helena copies. Arrows in red indicate strong-signaled bands; arrows in black indicate faint bands. (TIFF)
Data
Helena expression results in parental species. (Fig A and B) Helena expression rates relative to rp49 housekeeping gene in D. koepferae (Dko) and D. buzzatii (Dbu) somatic tissues (A) and gonads (B). Male samples are represented in blue and female samples are represented in brown. Boxes are determined by the first and third quartile values, with an...
Data
Summary of BLAST alignment results between Helena sequenced copies. Dbu = D. buzzatii, Dko28 = D. koepferae-28, Dko35-1 = D. koepferae-35-1, Dko35-2 = D. koepferae-35-2. (PDF)
Data
Variance comparisons of Helena expression rates between each hybrid generation and parental species. W = Levene’s test for equality of variances satistic, p-value = probability. *: p-value<0.05, **: p-value<0.01, ***: p-value<0.001. In red, p-values that are significant after Bonferoni correction (p-value<0.01). Each kind of sample (males, females,...
Data
FISH of Helena RNA expression in different F1 hybrid testes. Red staining are Helena transcripts, green staining is tissue autofluorescence. Arrows mark the presence of Helena transcripts. (TIFF)
Data
Alignment of Helena sequences (in fasta format) obtained with MAFFT E-INS-i algorithm and cleaned using Gblocks. This alignment was used to construct the phylogenetic tree on Fig 2. (PDF)
Data
Graphical representation of the Helena alignment obtained with MAFFT E-INS-i algorithm and cleaned using Gblocks. Highly conserved residues (similarity score per position > 0.5) are framed in blue and used to build the consensus sequence. Each nitrogenous base in a conserved position is represented in a different colour. (PDF)
Data
Summary of ΔCT values for all studied replicates (from different crosses) of each kind of sample for all generations. (PDF)
Article
Full-text available
Hybridization between different genomes is a source of genomic instability, sometimes associated with transposable element (TE) mobilization. Previous work showed that hybridization between the species Drosophila buzzatii and Drosophila koepferae induced mobilization of different (TEs), the Osvaldo retrotransposon being the most unstable. However,...
Article
Full-text available
Transposable elements (TEs) are DNA sequences able to be mobilized in host genomes. They are currently recognized as the major mutation inducers because of their insertion in the target, their effect on neighboring regions, or their ectopic recombination. A large number of factors including chemical and physical factors as well as intraspecific cro...
Article
Full-text available
Hybridization between species is a genomic instability factor involved in increasing mutation rate and new chromosomal rearrangements. Evidence of a relationship between interspecific hybridization and transposable element mobilization has been reported in different organisms, but most studies are usually performed with particular TEs and do not di...
Article
Full-text available
Transposable elements (TEs), by their capacity of moving and inducing mutations in the genome, are considered important drivers of species evolution. The successful invasions of TEs in genomes, despite their mutational properties, are an apparent paradox. TEs' transposition is usually strongly regulated to low value, but in some cases these element...
Article
Full-text available
Transposable elements (TEs) constitute an important source of genetic variability owing to their jumping and regulatory properties, and are considered to drive species evolution. Several factors that are able to induce TE transposition in genomes have been documented (for example environmental stress and inter- and intra-specific crosses) but in ma...
Article
Full-text available
An adapted amplified fragment length polymorphism (AFLP) protocol is presented for detection of hybrid instability in the genome of interspecific hybrids between Drosophila buzzatii and D. koepferae species. Analyses of 15 AFLP instability markers (new bands detected in hybrids) show that up to 81% are the result of transposable element (TE) activi...
Data
Poisson distribution: raw data of bilbo copy number per chromosome and population, P values and chi tests. A table of detailed tests of Poisson distribution of bilbo per chromosome and haploid genome.
Data
Poisson distribution: raw data of gypsy copy number per chromosome and population, P values and chi tests. A table of detailed tests of Poisson distribution of gypsy per chromosome and haploid genome.
Article
Full-text available
Transposable elements (TEs) constitute a substantial amount of all eukaryotic genomes. They induce an important proportion of deleterious mutations by insertion into genes or gene regulatory regions. However, their mutational capabilities are not always adverse but can contribute to the genetic diversity and evolution of organisms. Knowledge of the...
Article
Full-text available
Previous work on transposable element distribution in colonizing populations of Drosophila buzzatii revealed a high frequency of occupancy in several chromosomal sites. Two explanatory hypotheses were advanced: the founder hypothesis, by which founder genetic drift was responsible, and the unstable hypothesis that assigns this unusual distribution...
Article
Full-text available
A new transposable element, Isis, is identified as a LTR retrotransposon in Drosophila buzzatii. DNA sequence analysis shows that Isis contains three long ORFs similar to gag, pol and env genes of retroviruses. The ORF1 exhibits sequence homology to matrix, capsid and nucleocapsid gag proteins and ORF2 encodes a putative protease (PR), a reverse tr...
Article
Full-text available
Chromosomal distribution of transposable elements (TEs) Osvaldo and blanco in D. buzzatii was studied in three original natural populations from Argentina (Berna, Puerto Tirol and La Nostalgia) and a colonizer population from the Iberian Peninsula (Carboneras). The Spanish population showed significant differences for Osvaldo and blanco copy number...
Article
Full-text available
The behaviour of the retrotransposons copia and mdg1 was analysed in hybrids between Drosophila melanogaster and D. simulans. Females of a highly inbred line of D. melanogaster were crossed with D. simulans males from three natural populations. The insertion site profiles for the two elements were determined in F1 hybrid larvae by in situ hybridiza...
Article
Full-text available
In situ hybridization on polytene chromosomes of Drosophila melanogaster was used to compare the insertion patterns of copia and mdg1 transposable elements on chromosome 2 in male gametes sampled by two different methods: (i) by crossing the males tested with females from a highly inbred line with known copia and mdg1 insertion profiles; (ii) by cr...
Article
Full-text available
The insertion site polymorphism of the copia, mdg1, mdg3, gypsy, and P transposable elements was analysed by in situ hybridization to the polytene chromosomes in genomes of males from a natural population of Drosophila melanogaster. Parameters of various theoretical models of the population biology of transposable elements were estimated from our d...
Article
Full-text available
We have analysed by in situ hybridization the insertion site polymorphism of the copia, mdg1, mdg3, and P transposable elements in diploid genomes of wild males from a natural population of Drosophila melanogaster. The values of observed average degree of individual heterozygosity for all elements except mdg3 deviate statistically from the values e...
Article
A highly inbred line of Drosophila melanogaster, stable for the insertion pattern of the transposable elements copia and mdg1, was experimentally contaminated by flies from another line. We show that the alien genome income is clearly detectable by the changes induced in the insertion profiles of transposable elements, even twenty generations later...

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