Pierre Pétriacq

University of Bordeaux/ INRAE Bordeaux Aquitaine · Bordeaux Metabolome Facility

Plants deposit photosynthetically-fixed carbon in the rhizosphere, the thin soil layer directly around the root, thereby creating a hospitable environment for microbes. To manage the inhabitants of this nutrient-rich environment, plant roots exude and dynamically adjust microbe-attracting and -repelling compounds to stimulate specific members of th...

Ascorbate (vitamin C) is one of the most essential antioxidants in fresh fruits and vegetables. To get insights into the regulation of ascorbate metabolism in plants, a mutant producing ascorbate-enriched fruits was studied. The causal mutation, identified by a mapping-by-sequencing strategy, corresponded to a knock-out recessive mutation in a new...

Strawberry tree (Arbutus unedo) is a small resilient species with a circum-Mediterranean distribution, high ecological relevance in southern European forests and with several economical applications. As most orchards are usually installed on marginal lands where plants usually face severe drought, selecting plants that can better cope with water re...

Ammonium (NH4+)‐based fertilization efficiently mitigates the adverse effects of nitrogen fertilization on the environment. However, high concentrations of soil NH4+ provoke growth inhibition partly caused by the reduction of cell enlargement and associated with modifications of cell composition, such as an increase of sugars and a decrease in orga...

IntroductionAccuracy of feature annotation and metabolite identification in biological samples is a key element in metabolomics research. However, the annotation process is often hampered by the lack of spectral reference data in experimental conditions, as well as logistical difficulties in the spectral data management and exchange of annotations...

Current crop yield of the best ideotypes is stagnating and threatened by climate change. In this scenario, understanding wild plant adaptations in extreme ecosystems offers an opportunity to learn about new mechanisms for resilience. Previous studies have shown species specificity for metabolites involved in plant adaptation to harsh environments....

During its development, the leaf undergoes profound metabolic changes to ensure, among other things, its growth. The subcellular metabolome of tomato leaves was studied at four stages of leaf development, with a particular emphasis on the composition of the vacuole, a major actor of cell growth. For this, leaves were collected at different position...

Climate change and an increasing population, present a massive global challenge with respect to environmentally sustainable nutritious food production. Crop yield enhancements, through breeding, are decreasing, whilst agricultural intensification is constrained by emerging, re-emerging, and endemic pests and pathogens, accounting for ~30% of global...

Among crop fruit trees, the apricot (Prunus armeniaca) provides an excellent model to study divergence and adaptation processes. Here, we obtain nearly 600 Armeniaca apricot genomes and four high-quality assemblies anchored on genetic maps. Chinese and European apricots form two differentiated gene pools with high genetic diversity, resulting from...

The environmental fluctuations of a constantly evolving world can mould a changing context, often unfavourable to sessile organisms that must adjust their resource allocation between both resistance or tolerance mechanisms and growth. Plants bear the fascinating ability to survive and thrive under extreme conditions, a capacity that has always attr...

Volume 67 of Advances in Botanical Research “Metabolomics coming of age with its technological diversity” edited by Dominique Rolin (series Editors Jean-Pierre Jacquot and Pierre Gadal) was published in 2013 and featured 13 chapters including more than 40 authors with a range of expertise in biology, biochemistry, analytical chemistry, chemometrics...

Ascorbate is a major antioxidant buffer in plants, so several approaches have been developed to increase the ascorbate contents of fruits and vegetables. In this study, we combined forward genetics with mapping-by-sequencing approaches using an EMSMicro-Tom population to identify putative regulators underlying a high ascorbate phenotype in fruits....

Plant metabolomics is a set of fast-moving, analytical and chemometric tools and methods for plant functional genomics, phenotyping and systems biology. This multidisciplinary “omics” science can deliver qualitative and quantitative data that provide a detailed description of biochemical systems that are influenced by environmental changes, such as...

Metabolomics has become essential in the field of phenotyping, providing information halfway between genomes and phenotypes that can be used to predict complex traits. Thus in plants, studies have shown that metabolomics can be used to predict complex traits such as leaf biomass or yield. From a fundamental point of view, these advances can be used...

Fruit quality, that remains crucial to human nutrition and health, is closely related to biochemical composition, mostly, although not only, due to low molecular-weight metabolites. Final fruit quality results from coordinated physiological processes during development from anthesis to growth and ripe stages. Fruit development and quality may be mo...

Over the past 10 years, knowledge about several aspects of fruit metabolism has been greatly improved. Notably, high-throughput metabolomic technologies have allowed quantifying metabolite levels across various biological processes, and identifying the genes that underly fruit development and ripening. This Special Issue is designed to exemplify th...

In response to various stimuli, plants acquire resistance against pests and/or pathogens. Such acquired or induced resistance allows plants to rapidly adapt to their environment. Spraying the bark of mature Norway spruce (Picea abies) trees with the phytohormone methyl jasmonate (MeJA) enhances resistance to tree‐killing bark beetles and their asso...

Tomato is a major crop suffering substantial yield losses from diseases, as fruit decay at a postharvest level can claim up to 50% of the total production worldwide. Due to the environmental risks of fungicides, there is an increasing interest in exploiting plant immunity through priming, which is an adaptive strategy that improves plant defensive...

UMR BFP - Equipe Métabolisme

Central metabolism is the engine of plant biomass, supplying fruit growth with building blocks, energy, and biochemical cofactors. Among metabolic cornerstones, nicotinamide adenine dinucleotide (NAD) is particularly pivotal for electron transfer through reduction–oxidation (redox) reactions, thus participating in a myriad of biochemical processes....

Plant central metabolism generates reactive oxygen species (ROS), which are key regulators that mediate signalling pathways involved in developmental processes and plant responses to environmental fluctuations. These highly reactive metabolites can lead to cellular damage when the reduction-oxidation (redox) homeostasis becomes unbalanced. Whilst d...

The analysis of chemical diversity in non-sterile rhizosphere soil has been a pressing methodological challenge for years. Rhizosphere-enriched chemicals (i.e., rhizochemicals) include root exudation chemicals, (microbial) breakdown products thereof, and de novo produced metabolites by rhizosphere-inhabiting microbes, all of which can play an impor...

Protein synthesis and degradation are essential processes that regulate cell status. Because labeling in bulky organs, such as fruits, is difficult, we developed a modeling approach to study protein turnover at the global scale in developing tomato (Solanum lycopersicum) fruit. Quantitative data were collected for transcripts and proteins during fr...

The rhizobiome is an important regulator of plant growth and health. Plants shape their rhizobiome communities through production and release of primary and secondary root metabolites. Benzoxazinoids (BXs) are common tryptophan-derived secondary metabolites in grasses that regulate belowground and aboveground biotic interactions. In addition to the...

Many metabolic processes that occur in living cells involve oxidation-reduction (redox) chemistry underpinned by compounds, such as glutathione, ascorbate, and/or pyridine nucleotides. Among these redox carriers, nicotinamide adenine dinucleotide (NAD⁺) represents one of the cornerstones of cellular oxidation and is essential for plant growth and d...

Concerns over rising atmospheric CO2 concentrations have led to growing interest in the effects of global change on plant-microbe interactions. As a primary substrate of plant metabolism, atmospheric CO2 influences below-ground carbon allocation and root exudation chemistry, potentially affecting rhizosphere interactions with beneficial soil microb...

Humanity faces the challenge of having to increase food production to feed an exponentially growing world population, while crop diseases reduce yields to levels that we can no longer afford. Besides, a significant amount of waste is produced after fruit harvest. Fruit decay due to diseases at a post-harvest level can claim up to 50% of the total p...

Fig. S1 Effects of CO2 on plant development. Fig. S2 Images of Hpa colonisation classes. Fig. S3 qPCR‐based quantification of Hpa and Pc biomass. Fig. S4 Role of SA signalling in saCO2‐induced resistance against Hpa. Fig. S5 Global metabolic signatures of Hpa‐inoculated Arabidopsis at saCO2 and aCO2. Fig. S6 Selection of ions that are induced...

- β-aminobutyric acid (BABA) induces broad-spectrum disease resistance, but also represses plant growth, which has limited its exploitation in crop protection. BABA perception relies on binding to the aspartyl-tRNA synthetase (AspRS) IBI1, which primes the enzyme for secondary defense activity. This study aimed to identify structural BABA analogues...

Since its discovery more than a century ago, nicotinamide adenine dinucleotide (NAD+) is recognised as a fascinating cornerstone of cellular metabolism. This ubiquitous energy cofactor plays vital roles in metabolic pathways and regulatory processes, a fact emphasised by the essentiality of a balanced NAD+ metabolism for normal plant growth and dev...

The impacts of rising atmospheric CO2 concentrations on plant disease have received increasing attention, but with little consensus emerging on the direct mechanisms by which CO2 shapes plant immunity. Furthermore, the impact of sub‐ambient CO2 concentrations, which plants have experienced repeatedly over the past 800 000 yr, has been largely overl...

Figure S7. Details of quantitative differences in metabolites.

Appendix S1. Supplementary experimental procedures.

Figure S2. Relative abundance of bacterial taxa.

Figure S3. Solvent polarity and extraction of rhizosphere chemistry.

Figure S4. Epifluorescence microscopy analysis of Arabidopsis root cell damage.

Figure S6. Binary PLS‐DA analysis of metabolite profiles.

Figure S8. Relative quantities of benzoxazinoids.

Figure S1. Rarefaction curves of 16S rRNA operational taxonomic units (OTUs).

Figure S9. Profiling distal rhizosphere chemistry.

Table S1. Putative identification of Arabidopsis metabolic markers.

Figure S5. Reproducibility of metabolite profiles between experiments.

Table S2. Putative identification of maize metabolic markers.

Rhizosphere chemistry is the sum of root exudation chemicals, their breakdown products and microbial products of soil-derived chemicals. To date, most studies about root exudation chemistry are based on sterile cultivation systems, which limits the discovery of microbial breakdown products that act as semiochemicals and shape microbial rhizosphere...

Minimising losses to pests and diseases is essential for producing sufficient food to feed our rapidly growing population. The necrotrophic fungus Botrytis cinerea triggers devastating pre- and post-harvest yield losses in tomato (Solanum lycopersicum). Current control methods are based on the pre-harvest use of fungicides, which are limited by str...

By investigating the metabolism of plants infected by Plasmodiophora brassicae, the agent of clubroot disease, this paper shows the changes in plant metabolites depending on responses of the plant to infection. A nontargeted metabolomic approach was used to obtain metabolic ‘fingerprints’, which were then combined with host transcriptomic data. At...

The production of reactive oxygen species (ROS) is one of the first defense reactions induced in Arabidopsis in response to infection by the pectinolytic enterobacterium Dickeya dadantii. Previous results also suggest that abscisic acid (ABA) favors D. dadantii multiplication and spread into its hosts. Here, we confirm this hypothesis using ABA-def...

Metabolites extraction and subsequent metabolic fingerprinting using plant leaf material.

Plant mutants for genes encoding subunits of mitochondrial complex I (CI; NADH:ubiquinone oxidoreductase), the first enzyme of the respiratory chain, display various phenotypes depending on growth conditions. Here, we examined the impact of photoperiod, a major environmental factor controlling plant development, on two Arabidopsis (Arabidopsis thal...

Environmental constraints, such as biotic stress, are detrimental for plant productivity, survival and reproduction. Although plants have evolved metabolic mechanisms to tolerate environmental challenges, our knowledge on the importance of mitochondrial metabolism in biotic stress responses is still fragmentary. This study examined the effects of m...

Plant mutants for genes encoding subunits of mitochondrial Complex I (CI, NADH:ubiquinone oxidoreductase), the first enzyme of the respiratory chain, display various phenotypes depending on growth conditions. Here, we examined the impact of photoperiod, a major environmental factor controlling plant development, on two Arabidopsis thaliana CI mutan...

NAD is a pyridine nucleotide that is involved in cell metabolism and signaling of plant growth and stress. Recently, we reported on the multifaceted nature of NAD-inducible immunity in Arabidopsis. We identified NAD as an integral regulator of multiple defense layers such as production of ROS, deposition of callose, stimulation of cell death and mo...

Pyridine nucleotides, such as nicotinamide adenine dinucleotide (NAD), are crucial redox carriers and have emerged as important signaling molecules in stress responses. Previously, we have demonstrated in Arabidopsis thaliana (Arabidopsis) that the inducible NAD-overproducing nadC lines are more resistant to an avirulent strain of Pseudomonas syrin...

Necrotrophic and biotrophic pathogens are resisted by different plant defenses. While necrotrophic pathogens are sensitive to jasmonic acid (JA)-dependent resistance, biotrophic pathogens are resisted by salicylic acid (SA)- and reactive oxygen species (ROS)-dependent resistance. Although many pathogens switch from biotrophy to necrotrophy during i...

Phytopathogenic microbes are commonly classified as necrotrophs or biotrophs depending whether they proliferate from dead tissues or feed from living cells. Some pathogens are able to adopt both trophic lifestyles. Complex molecular and biochemical networks have been characterized for necrotrophic and biotrophic infections, but little is known whic...

As sessile organisms, plants must adapt to changing environmental conditions that affect their optimal growth and development. Recent studies have highlig hted the importance of a central molecule in redox and respiratory metabolism, nicotinamide aden ine dinucleotide (NAD), in the metabolic signaling in plants and animals. They suggest that NAD pl...

Priming is a long-term immune response that prepares the plant immune system against upcoming attack by pests and diseases1. However, development of disease management strategies that exploit priming is hampered by the fact that over-stimulation by chemical priming agents can have adverse effects on plant growth and development2. Broad-spectrum def...

Specific chemicals can prime the plant immune system for augmented defense. β-aminobutyric acid (BABA) is a priming agent that provides broad-spectrum disease protection. However, BABA also suppresses plant growth when applied in high doses, which has hampered its application as a crop defense activator. Here we describe a mutant of Arabidopsis tha...

Many metabolic processes that occur in living cells involve oxido-reduction (redox) chemistry underpinned by redox compounds such as glutathione, ascorbate and/or pyridine nucleotides. Among these redox carriers, nicotinamide adenine dinucleotide (NAD) is the cornerstone of cellular oxidations along catabolism and is therefore essential for plant g...