Pierre Cardol

Pierre Cardol
University of Liège | ulg · Department of Life Sciences

PhD & Research Professor

About

147
Publications
19,887
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
6,625
Citations
Introduction
The overall objective of our researches is the understanding of the mechanisms regulating mitochondrial and photosynthetic electron transport chains, and their interactions.
Additional affiliations
January 2010 - present
University of Liège
Position
  • Lecturer
Description
  • Bioenergetics
October 2006 - December 2007
Institute of Physical and Chemical Biology
Position
  • PostDoc Position
May 2006 - August 2006
Universidad Nacional Autónoma de México

Publications

Publications (147)
Article
Full-text available
Global energy demand and fossil fuels impact on climate can be partially managed by an increase in the use of biofuels for transports and industries. Biodiesel production is generally preceded by a transesterification process of the green biomass triacylglycerols that generates large amounts of glycerol as a by-product. In this study, the extremoph...
Article
Full-text available
Bright field microscopes are particularly useful tools for biologists for cell and tissue observation, phenotyping, cell counting, and so on. Direct cell observation provides a wealth of information on cells’ nature and physiological condition. Microscopic analyses are, however, time-consuming and usually not easy to parallelize. We describe the fa...
Article
Mitochondrial F1FO-ATP synthase plays a key role in cellular bioenergetics; this enzyme is present in all eukaryotic linages except in amitochondriate organisms. Despite its ancestral origin, traceable to the alpha proteobacterial endosymbiotic event, the actual structural diversity of these complexes, due to large differences in their polypeptide...
Preprint
teaserEuglenoids show great promise to benefit our world; as biofuels, environmental remediators, anti-cancer agents, robotics design simulators and food nutritional agents, but the absence of reference genomes currently limit realizing these benefits. The Euglena International Network (EIN) (https://euglenanetwork.org/) aims to address these chall...
Article
Several phytoplankton species produce the metabolites dimethylsulfoniopropionate (DMSP) and dimethylsulfoxide (DMSO) but their intracellular roles need to be better understood. To improve the understanding of the DMSP antioxidant function suggested by Sunda et al. (2002), we exposed the diatom Skeletonema costatum, the Prymnesiophyceae Phaeocystis...
Article
Full-text available
Increasing industrial and anthropogenic activities are producing and releasing more and more pollutants in the environment. Among them, toxic metals are one of the major threats for human health and natural ecosystems. Because photosynthetic organisms play a critical role in primary productivity and pollution management, investigating their respons...
Article
Full-text available
The coupling between mitochondrial respiration and photosynthesis plays an important role in the energetic physiology of green plants and some secondary‐red photosynthetic eukaryotes (diatoms), allowing an efficient CO2 assimilation and optimal growth. ●Using the flagellate Euglena gracilis, we first tested if photosynthesis‐respiration coupling oc...
Article
Full-text available
In vivo associations of respiratory complexes forming higher supramolecular structures are generally accepted nowadays. Supercomplexes (SC) built by complexes I, III and IV and the so-called respirasome (I/III2/IV) have been described in mitochondria from several model organisms (yeasts, mammals and green plants), but information is scarce in other...
Article
Full-text available
Euglena gracilis is a well-known photosynthetic microeukaryote considered as the product of a secondary endosymbiosis between a green alga and a phagotrophic unicellular belonging to the same eukaryotic phylum as the parasitic trypanosomatids. As its nuclear genome has proven difficult to sequence, reliable transcriptomes are important for function...
Article
Full-text available
Many routines in biological experiments require the precise handling of liquid volumes in the range of microliters up to liters. In this paper, we describe a new wireless controller that is adapted to liquid manipulation tasks, in particular when combined with the proposed 3D-printed pumps. It can be built from widely available electronic component...
Article
Full-text available
Abstract The mutualistic relationship existing between scleractinian corals and their photosynthetic endosymbionts involves a complex integration of the metabolic pathways within the holobiont. Respiration and photosynthesis are the most important of these processes and although they have been extensively studied, our understanding of their interac...
Article
Disentangling the metabolic functioning of corals’ endosymbionts (Symbiodiniaceae) is relevant to understand the response of coral reefs to warming oceans. In this work, we first questioned if there is an energetic coupling between photosynthesis and respiration in Symbiodiniaceae (Symbiodinium, Durusdinium and Effrenium), and second, how different...
Conference Paper
Full-text available
The transfer of photosynthesis from eukaryotes that belongs to Archaeplastidae (i.e. green algae or red algae) to other non-photosynthetic organisms is among the most spectacular examples of evolutionary innovation. This transfer occurred many times upon evolution and gave rise to photosynthetic lineages of high ecological and/or biotechnological s...
Article
Full-text available
The breakdown of the symbiosis between cnidarians and Symbiodiniaceae often occurs upon periods of elevated sea surface temperature and gives rise to bleaching events that affect coral reefs worldwide. In this respect, an impairment of photosynthesis would be responsible for light-dependent generation of toxic reactive oxygen species putatively con...
Conference Paper
Full-text available
Keeping an algal culture at a constant turbidity requires expensive and complex devices. We designed a low-cost, user friendly but also highly configurable phototurbidostat using 3D-printing, open-source software and electronics. The device is able to monitor in real time a culture in photobioreactor, and dynamically adjust the conditions to mainta...
Article
Full-text available
The sea anemone Entacmaea medusivora (Actiniaria, Anthozoa) commonly feeds on the golden jellyfish Mastigias papua (Rhizostomeae, Scyphozoa) which harbours endosymbiotic dinoflagellate of the genus Cladocopium (Symbiodiniaceae). In this study, we monitored the photosynthetic activity of the endosymbiotic microalgae while their host jellyfish were i...
Article
Apart from the canonical light-driven linear electron flow (LEF) from water to CO2, numerous regulatory and alternative electron transfer pathways exist in chloroplasts. One of them is the cyclic electron flow around Photosystem I (CEF), contributing to photoprotection of both Photosystem I and II (PSI, PSII) and supplying extra ATP to fix atmosphe...
Article
Cyclic electron flow (CEF) is defined as a return of the reductants from the acceptor side of Photosystem I (PSI) to the pool of its donors via the cytochrome b 6 f. It is described to be complementary to the linear electron flow and essential for photosynthesis. However, despite many efforts aimed to characterize CEF, its pathway and its regulatio...
Article
Full-text available
Menaquinone (vitamin K2) shuttles electrons between membrane-bound respiratory complexes under microaerophilic conditions. In photosynthetic eukaryotes and cyanobacteria, phylloquinone (vitamin K1) participates in photosystem I function. Here we elucidate the evolutionary history of vitamin K metabolism in algae and plants. We show that Chlamydiale...
Article
Full-text available
In mitochondrial oxidative phosphorylation, electron transfer from NADH or succinate to oxygen by a series of large protein complexes in the inner mitochondrial membrane (complexes I-IV) is coupled to the generation of an electrochemical proton gradient, the energy of which is utilized by complex V to generate ATP. In Euglena gracilis, a non-parasi...
Article
In changing light conditions, photosynthetic organisms develop different strategies to maintain a fine balance between light harvesting, photochemistry, and photoprotection. One of the most widespread photoprotective mechanisms consists in the dissipation of excess light energy in the form of heat in the photosystem II antenna, which participates t...
Chapter
Mitochondrion is the site where the Krebs cycle and oxidative phosphorylation (OXPHOS) take place. After a brief overview of Krebs cycle and acetate metabolism in Chlamydomonas, this chapter focuses on OXPHOS components. OXPHOS is composed of five major multiprotein complexes: NADH:ubiquinone oxidoreductase (complex I), succinate dehydrogenase (com...
Article
The qualitative screening method to select complex I mutants in the microalga Chlamydomonas, based on reduced growth under heterotrophic condition, is not suited for high throughput screening. In order to develop a fast screening method based on measurements of chlorophyll fluorescence, we first demonstrated that complex I mutants displayed decreas...
Preprint
Full-text available
Cyclic electron flow (CEF), one of the major alternative electron transport pathways to the primary linear electron flow (LEF) in chloroplasts has been discovered in the middle of the last century. It is defined as a return of the reductants from the acceptor side of the Photosystem I (PSI) to the pool of its donors via the cytochrome b 6 f , and h...
Article
Understanding of the molecular mechanisms of photosynthetic electron and proton transports and their regulation in plants and algae in response to changes in environmental conditions is an important issue for fundamental research on photosynthesis, and may extend even to practical applications by identifying important sites for improvement of photo...
Article
Mitochondrial F1FO-ATP synthase of the chlorophycean algae Polytomella sp. can be isolated as a highly stable dimeric complex of 1600 kDa. It is composed of eight highly conserved orthodox subunits (α, β, γ, δ, ε, OSCP, a, and c) and nine subunits (Asa1–9) that are exclusive of chlorophycean algae. The Asa subunits replace those that build up the p...
Article
Mitochondrial F1Fo-ATP synthase (complex V) is an oligomeric complex that exhibits a molecular mass around 550 kDa. The F1Fo-ATP synthase of the green chlorophycean alga Chlamydomonas reinhardtii and of its close colorless relative Polytomella sp. is isolated as a highly-stable dimer of 1600 kDa after solubilization of algal mitochondria with laury...
Article
Full-text available
Mitochondrial respiratory-chain complexes from Euglenozoa comprise classical subunits described in other eukaryotes (i.e. mammals and fungi) and subunits that are restricted to Euglenozoa (e.g. Euglena gracilis and Trypanosoma brucei). Here we studied the mitochondrial F1FO-ATP synthase (or Complex V) from the photosynthetic eukaryote E. gracilis i...
Data
Figure S6. Photosystem (PS) I and PSII content in control and mutant strains.
Data
Figure S2. Amplification of MENB and MENE genes and their flanking regions.
Data
Figure S3. Chlorophyll fluorescence induction curves of wild‐type and complemented strains.
Data
Figure S5. Determination of photosystem (PS) I and PSII antenna size in wild‐type, complemented and men mutant strains.
Data
Figure S9. Sequence alignment of CYP25 protein from Chlamydomonas reinhardtii (CrCYP25) with its two homologs in humans (HsCYP4F2 and CYP4F11).
Data
Figure S1. Thermal asymmetric interlaced‐PCR product sequences.
Data
Figure S4. Relative content of photosystem I.
Data
Figure S7. RT‐PCR analysis of menc and mend mutants.
Data
Figure S8. Sequence alignment of PHYLLO protein from Chlamydomonas reinhardtii (CrPHYLLO) and Arabidopsis thaliana (AtPHYLLO) with Escherichia coli MenF, MenD, MenC and MenH proteins (EcMenF, ‐D, ‐C, ‐H).
Data
Table S1. Co‐segregation analysis of the antibiotic resistance cassette with the fluorescence phenotype.
Data
Appendix S1. Detailed description of thermal asymmetric interlaced‐PCR analyses.
Article
Full-text available
Photosynthetic organisms are exposed to drastic changes in light conditions, which can affect their photosynthetic efficiency and can induce photodamage. To face these changes they have developed a series of acclimation mechanisms. In this work we have studied the acclimation strategies of Chlamydomonas reinhardtii, a model green alga that can grow...
Article
Full-text available
Phylloquinone (PhQ) or Vitamin K1 is an essential electron carrier (A1 ) in photosystem I (PSI). In the green alga Chlamydomonas reinhardtii, which is a model organism for photosynthesis study, a detailed characterization of the pathway is missing with only one mutant deficient for MEND that has been analyzed. We took advantage of the fact that a d...
Article
Full-text available
The algae Chlamydomonas reinhardtii and Polytomella sp., a green and a colorless member of the chlorophycean lineage respectively, exhibit a highly-stable dimeric mitochondrial F1Fo-ATP synthase (complex V), with a molecular mass of 1600 kDa. Polytomella, lacking both chloroplasts and a cell wall, has greatly facilitated the purification of the alg...
Article
The algae Chlamydomonas reinhardtii and Polytomella sp., a green and a colorless member of the chlorophycean lineage respectively, exhibit a highly-stable dimeric mitochondrial F1Fo-ATP synthase (complex V), with a molecular mass of 1600 kDa. Polytomella, lacking both chloroplasts and a cell wall, has greatly facilitated the purification of the alg...
Article
Mitochondrial F1FO-ATP synthase of chlorophycean algae is dimeric. It contains eight orthodox subunits (alpha, beta, gamma, delta, epsilon, OSCP, a and c) and nine atypical subunits (Asa1 to 9). These subunits build the peripheral stalk of the enzyme and stabilize its dimeric structure. The location of the 66.1kDa subunit Asa1 has been debated. On...
Article
Full-text available
Diatoms are one of the most ecologically successful classes of photosynthetic marine eukaryotes in the contemporary oceans. Over the past 30 million years, they have helped to moderate Earth’s climate by absorbing carbon dioxide from the atmosphere, sequestering it via the biological carbon pump and ultimately burying organic carbon in the lithosph...
Article
Full-text available
During the last decades, coral reefs have been affected by several large-scale bleaching events, and such phenomena are expected to increase in frequency and severity in the future, thus compromising their survival. High sea surface temperature accompanied by high levels of solar irradiance has been found to be responsible for the induction of oxid...
Article
Full-text available
The model green microalga Chlamydomonas reinhardtii is frequently subject to periods of dark and anoxia in its natural environment. Here, by resorting to mutants defective in the maturation of the chloroplastic oxygen-sensitive hydrogenases or in Proton-Gradient Regulation-Like1 (PGRL1)-dependent cyclic electron flow around photosystem I (PSI-CEF),...
Article
The unicellular green alga Chlamydomonas reinhardtii is a model organism for studying energetic metabolism. Most mitochondrial respiratory-deficient mutants characterized to date have been isolated on the basis of their reduced ability to grow in heterotrophic conditions. Mitochondrial deficiencies are usually partly compensated by adjustment of ph...
Conference Paper
Full-text available
Euglena gracilis, a non-parasitic secondary green alga related to trypanosomes, has a complex mitochondrial oxidative phosphorylation system constituted by atypical respiratory enzymes (complexes I-V). Recently, the analysis of the subunit composition of respiratory complexes by 2D BN/SDS PAGE has shown that at least 41 of the non-canonical subunit...
Article
Full-text available
Photosynthetic microalgae are exposed to changing environmental conditions. In particular, microbes found in ponds or soils often face hypoxia or even anoxia and this severely impacts their physiology. Chlamydomonas reinhardtii is one amongst such photosynthetic microorganisms recognized for its unusual wealth of fermentative pathways and the exten...