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Pierre Bensidoun currently works at the Research Unit in Ribonucleoprotein Biochemistry, Institut de recherches cliniques de Montréal. Pierre does research in Cell Biology, Genetics and Molecular Biology. Their most recent publication is 'Imaging single mRNAs to study dynamics of mRNA export in the yeast Saccharomyces cerevisiae'.
To determine which transcripts should reach the cytoplasm for translation, eukaryotic cells have established mechanisms to regulate selective mRNA export through the nuclear pore complex (NPC). The nuclear basket, a substructure of the NPC protruding into the nucleoplasm, is thought to function as a stable platform where mRNA-protein complexes (mRN...
The nuclear pore complex (NPC) serves as a central gate for mRNAs to transit from the nucleus to the cytoplasm. The ability for mRNAs to get exported is linked to various upstream nuclear processes including co‐transcriptional RNP assembly and processing, and only export competent mRNPs are thought to get access to the NPC. While the nuclear pore i...
Single-molecule resolution imaging has become an important tool in the study of cell biology. Aptamer-based approaches (e.g., MS2 and PP7) allow for detection of single RNA molecules in living cells and have been used to study various aspects of mRNA metabolism, including mRNP nuclear export. Here we outline an imaging protocol for the study of int...
Regulation of mRNA and protein expression occurs at many levels, initiated at transcription and followed by mRNA processing, export, localization, translation and mRNA degradation. The ability to study mRNAs in living cells has become a critical tool to study and analyze how the various steps of the gene expression pathway are carried out. Here we...
After synthesis and transit through the nucleus, messenger RNAs (mRNAs) are exported to the cytoplasm through the nuclear pore complex (NPC). At the NPC, messenger ribonucleoproteins (mRNPs) first encounter the nuclear basket where mRNP rearrangements are thought to allow access to the transport channel. Here, we use single mRNA resolution live cel...
So, I have 2 proteins let's named them A and B. In short, when A is the bait I can co-immunopurify B but when B is the bait I can't co-immunopurify A (or at least I can't see A by westernblot).
Both are tagged: A with Luciferase and B with GFP.
Also B could form tetramere with itself and A an homo dimere.
Have you already seen such situation? what could explain it ?
thank you for your help!