Phuc Van Pham

Phuc Van Pham
Stem Cell Institute, Ho Chi Minh City University of Science

MSc, PhD
Scale-up stem cells and stem cells derived exosomes for clinical application

About

252
Publications
66,324
Reads
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Introduction
Phuc Van Pham is currently a Professor of Biology at Vietnam National University Ho Chi Minh city. Currently, he is a Director of Stem Cell Institute, Director of Laboratory of Stem Cell Research and Application. He is a longstanding lecturer and translational scientist at the University, and is a member of several societies and journal editorial boards focused on stem cells and cancer biology.
Additional affiliations
January 2011 - present
Vietnam National University - Ho Chi Minh City
January 2010 - present
Vietnam National University - Ho Chi Minh
December 2009 - February 2016
Ho Chi Minh City University of Science
Position
  • Lecturer

Publications

Publications (252)
Article
Full-text available
Introduction: Embryonic stem cells are pluripotent, thus capable of differentiating into all types of cells derived from the three germ layers. However, the application of embryonic stem cells (ESCs) for preclinical and clinical studies is difficult due to ethical concerns. Induced pluripotent stem cells (iPSCs) are derived from differentiation and...
Chapter
Kidney disease arises from dysfunction of the kidneys due to a variety of factors, which impact the kidney but also kidney-related functions. There are, in fact, many forms of kidney or kidney-related diseases, including diabetic kidney disease, autoimmune disease, kidney transplantation, and chronic liver disease (CKD). Of the different types, CKD...
Article
Full-text available
Ischemia are common conditions related to lack of blood supply to tissues. Depending on the ischemic sites, ischemia can cause different diseases, such as hindlimb ischemia, heart infarction and stroke. This study aims to evaluate how extracellular vesicles (EVs) derived from ETV2 transfected fibroblasts affect endothelial cell proliferation and ne...
Article
Ischemia is the reduction of blood flow to tissues by injury of blood vessels. Depending on the sites of tissues and grade of ischemia, ischemia can cause many serious complications. This study aimed to evaluate the effects of the E-twenty six (ETS) factor Ets variant 2 (ETV2) gene expression in angiogenesis and the effect of ETV2 gene therapy in a...
Chapter
Avascular necrosis of the femoral head (AVNFH) is a condition involving low blood supply to the femoral head. Lacking blood, the femoral head degenerates. Although there are certain techniques to treat this disease, the treatment efficiency does not significantly improve the symptoms, especially in the late stage of AVNFH. Currently, AVNFH is treat...
Article
Full-text available
Immune cell therapy has been incorporated into cancer therapy over the past few years. Chimeric antigen receptor T cells (Car-T cells) transplantation is a novel and promising therapy for cancer treatment and introduces a new age of immune cell therapy. However, the expensive nature of genetic modification procedures limits the accessibility of Car...
Chapter
Introduction: In recent years, both stromal vascular fraction (SVF) from adipose tissue and mesenchymal stem cells (MSC) from adipose tissues were extensively used in both preclinical and clinical treatment for various diseases. Some studies reported differences in treatment efficacy between SVFs and MSCs in animals as well as in humans. Therefore...
Article
Full-text available
Breast cancer is the world's most common cancer in women and is the leading cause of their cancer-related mortality. Its early diagnosis with conventional therapies such as surgery, chemotherapy, and radiotherapy can give good results in most breast cancer patients. However, these therapies provide poor outcomes in metastatic breast cancers or late...
Article
Introduction: Adipose-derived stem cells (ADSCs) are mesenchymal stem cells (MSCs) that are found in adipose tissues, which are easily obtained from liposuction procedures using an enzyme mixture. The adhering cells are then selectively cultivated. ADSCs have great potential in regenerative medicine because they are plentiful, easily accessible, a...
Article
Introduction: Articular cartilage is limited in self-repair following injuries due to avascular, lymphatic, and nerve absence. Recent treatments for cartilage injuries, such as physical therapy, anti-inflammatory medication, chondrocyte implantation, and joint replacement, still have limitations. This study aimed to evaluate the treatment efficacy...
Article
BACKGROUND: Angiogenesis plays an important role in tissue repair. In recent years, one of the main research strategies used to induce angiogenesis in vivo has been via stem cell therapy, especially therapy using mesenchymal stem cells (MSCs). However, the use of MSCs in angiogenesis has also been limited due to the low angiogenic potency of MSCs....
Article
Introduction: The potency of mesenchymal stem cells (MSCs) related to their biological effects includes immune modulation and angiogenesis. Recently, some stress conditions were applied to enhance the angiogenic potential of MSCs. This study aimed to assess the effects of conditioned media (CM) collected from adipose-derived stem cells’ (ADSCs’) cu...
Article
Ischemic diseases are conditions associated with the restriction or blockage of blood supply to specific tissues. These conditions can cause moderate to severe complications in patients, and can lead to permanent disabilities. Since they are blood vessel-related diseases, ischemic diseases are usually treated with endothelial cells or endothelial p...
Article
Full-text available
Ocular chemical and thermal burns are frequent causes of hospitalization and require immediate interventions and care. Various surgical and pharmacological treatment strategies are employed according to damage severity. Controlling inflammation and neovascularization while promoting normal ocular surface anatomy and function restoration is the prin...
Article
Immunotherapy, especially immune cell-based therapy, is a strategy for cancer treatment that has over the past decades focused on novel modifications and targets. In recent years, adoptive cell immunotherapy has continuously evolved, with studies of different platforms utilizing different immune effector cells to kill a variety of cancer cells. Thi...
Article
Full-text available
Liver fibrosis (LF) mortality rate is approximately 2 million per year. Irrespective of the etiology of LF, a key element in its development is the transition of Hepatic stellate cells (HSCs) from a quiescent phenotype to a myofibroblast-like cell with the production of fibrotic proteins. It is necessary to define optimal isolation and culturing co...
Article
Wound healing, especially of chronic wounds, is still an unmet therapeutic area since assessment and management are extremely complicated. Although many efforts have been made to treat wounds, all strategies have achieved limited results for chronic wounds. Stem cell-based therapy is considered a promising approach for complex wounds such as those...
Article
Full-text available
Exosomes are small vesicles secreted by viable cells into the microenvironment. These vesicles bring various compositions, including lipids, RNAs and proteins, which carry information from producer cells to target cells. Cancer cells also produce exosomes, termed as tumor-derived exosomes (TDEs), which play important roles in immune modulation, ang...
Article
This study suggested a new method to produce the in vitro cartilage tissues by cartilage differentiation in spheroids from adipose-derived stem cells on porous scaffolds. Adipose-derived stem cells (ADSCs) were used to produce spheroids by the hanging-drop method. The spheroids were then loaded into a porous scaffold and induced to differentiate in...
Article
Full-text available
Brown and beige adipocytes have been widely known for their potential to dissipate excessive energy into heat form, resulting in an alleviation of obesity and other overweight-related conditions. This review highlights the origins, characteristics, and functions of the various kinds of adipocytes, as well as their anatomic distribution inside the h...
Article
Full-text available
Coronavirus disease-2019 (COVID-19) has affected more than 200 countries worldwide. This disease has hugely affected healthcare systems as well as the economy to an extent never seen before. To date, COVID-19 infection has led to about 165000 deaths in 150 countries. At present, there is no specific drug or efficient treatment for this disease. In...
Article
Full-text available
Anti-tumor activity screening is a typical process used in anti-tumor drug discovery. The ideal anti-tumor drug candidates are extracts or compounds that can inhibit the proliferation of cancer cells via apoptosis, while exerting minimal effects on normal somatic cells. For a long time, fibroblasts were used as normal cells for all anti-tumor scree...
Chapter
Introduction Medicinal plants have been used for disease treatment throughout history, especially in Asia. Vietnam is a tropical country which possesses forests with a diversity of plants; among the plants, many have been historically used as alternative therapies for various disease treatments. In this study we aimed to evaluate the selective cyto...
Article
Bioactivity-guided fractionation of the CHCl3-soluble extract of the roots of Paramignya trimera was carried out to obtain a new acridone alkaloid, paratrimerin I. Its structure was elucidated based on NMR spectroscopic data interpretation. Paratrimerin I showed noteworthy cytotoxicity against the HepG2 human hepatocellular and MCF-7 human breast c...
Chapter
Introduction The breast cancer cells with CD44⁺CD24⁻ phenotype are known to play an important role in tumorigenesis, drug resistance, and cancer recurrence. Breast cancer cells with CD44⁺CD24⁻ phenotype are cultured in three-dimensional (3D) stereotype showing the recapitulation of tumors in vivo such as cell differentiation, heterogeneity, and mic...
Chapter
The idea of utilizing the human immune system to eradicate tumors has been successfully practiced for the past decades, as reported in multiple published studies. Among cancer types, non-small cell lung cancer (NSCLC) is considered the most lethal type, leading to the necessity of finding an effective treatment for this category of cancer. Building...
Chapter
Introduction Medicinal plants have been used for disease treatment throughout history, especially in parts of Asia. Vietnam is a tropical country which possesses forests with a wide diversity of plants, which have a long history of being used as alternative remedies for treatment of various diseases. In this study, we aimed to test the bioactivity...
Article
Full-text available
Introduction: Xao Tam Phan (Paramignya trimera) has long been used in Viet Nam as an herbal medicine for the treatment of Hepatitis, hepatocellular carcinoma, and diabetes. This study aimed to determine the anti-proliferation effect of Paramignya trimera extract (P. trimera extract) on HepG2 hepatocellular carcinoma cells. Methods: AlamarBlue assay...
Article
Introduction: The monolayer cell culture model is a popular model for screening anti-tumor activity of plant extracts. However, almost the extracts selected for screening in this model have failed in subsequent animal models. Therefore, there is only about 5 % of candidates from the original thousands of drugs that are screened which ultimately rea...
Article
Introduction: Cancer cells rely on glycolysis to generate energy and synthesize biomass for cell growth and proliferation (the Warburg effect). Recent studies have shown that citrate has an inhibitory effect on several cancer cells, such as human gastric cancer and ovarian cancer, by inhibiting glycolysis. In this study, we investigated the effects...
Article
Introduction: Hepatocellular carcinoma (HCC) is one of the leading causes of cancer mortality in the world. Therefore, more and more studies are developing novel therapies to treat this disease. The pre-clinical trials on animals are a vital step to evaluate the efficacy as well as side effects of these novel therapies. Hence, this study aimed to d...
Article
Introduction: Ovarian cancer is one of the most common cancers in women. Due to the difficulty in early detection and treatment of ovarian cancer, many research studies and clinical trials have been developed to discover more efficient therapies. Besides Western medicine, traditional medicine has gained increased interest as a research field with p...
Article
Introduction: Recently reported data have suggested that only a small subset of cancer cells possess the capability to initiate malignancies. These observations were based on investigation of cells within the primary tumors displaying a distinct surface marker pattern. CD133 marker is a putative hematopoietic and neuronal stem cell marker, which is...
Article
Full-text available
Introduction: Chronic obstructive pulmonary disease (COPD) is the third leading cause of death worldwide. COPD results from chronic inflammation of the lungs. Current treatments, including physical and chemical therapies, provide limited results. Stem cells, particularly mesenchymal stem cells (MSCs), are used to treat COPD. Here, we evaluated the...
Book
This new series, based on a bi-annual conference and its topics, represents a major contribution to the emerging science of cancer research and regenerative medicine. Each volume brings together some of the most pre-eminent scientists working on cancer biology, cancer treatment, cancer diagnosis, cancer prevention and regenerative medicine to share...
Article
Full-text available
Introduction: Panax vietnamensis Ha et Grushv. (Ngoc Linh ginseng) – a new species recently discovered in Vietnam – has received much interest due to its rich content of saponins, including those unknown. This study assessed the effects of the Ngoc Linh ginseng extract fractions on proliferation and differentiation of cultured mouse neural stem cel...
Article
Objective: Cancer has been considered as one of the world's leading causes of death. Recently, the Paramignya trimera plant, locally called ``Xao Tam Phan'', has become a popular Vietnamese medicinal herb that is used as alternative medicine for cancer treatment support with minimal side effects. In this study, we aimed to demonstrate the cytotoxic...
Chapter
Autoimmune diseases (ADs) are common conditions of human health. These diseases can significantly reduce the patients’ quality of life. Although most autoimmune diseases can be controlled by certain immunosuppressive drugs, after long-term treatment, the therapeutic efficacy of these drugs can be significantly decreased while side effects may be in...
Chapter
Introduction: Human umbilical cord-derived mesenchymal stem cells (UCMSCs) are multiple potential stem cells that can differentiate into various kinds of functional cells, including adipocytes, osteoblasts, and chondroblasts. Thus, UCMSCs have recently been used in both stem cell therapy and tissue engineering applications to produce various functi...
Article
Introduction: The senescence of stem cells is the primary reason that causes aging of stem cell-containing tissues. Some hypotheses have suggested that high glucose concentration in diabetic patients is the main factor that causes senescence of cells in those patients. This study aimed to evaluate the effects of high glucose concentrations on the s...
Article
Introduction: In vitro cultivation of DCs and cytokine-induced killer cells (CIK cells) - a special phenotype of T lymphocyte populations — for cancer treatment has gained significant research interest. The goal of this study is to understand whether the priming from DCs helps CIK cells to exert their toxic function and kill the cancer cells. Metho...
Article
Introduction: Human mesenchymal stem cells (MSCs) are the most popular stem cells applied in disease treatment. MSCs can be isolated and in vitro expanded from various sources such as bone marrow, peripheral blood, umbilical cord blood, umbilical cord tissue, and adipose tissue. According to Dominici et al. (2006), MSCs should express CD105, an ess...
Article
In the previous publication with title ”A type 2 diabetes mellitus patient was successfully treated by autologous bone marrow-derived stem cell transplantation: A case report”1, page 2968, in the Table 1, the unit for fasting blood glucose and blood glucose 2 hours after meals is mg/dL; however, the corrected unit for fasting blood glucose and bloo...
Article
Introduction: Mesenchymal stem cells (MSCs) are possibly the most potent type of stem cells for the treatment of many diseases since they possess many advantageous properties, such as abundant source, ease of isolation, and potential to differentiate and trans-differentiate into different types of cells. Although the therapeutic potential of expand...
Article
Introduction: Type 2 diabetes mellitus (T2DM) iscurrently one of the most common diseases as a result of obesity and aging. Currently, T2DMis treated by various methods, such as insulin injection and glucose regulation agents. In this report, we report the case of a T2DM patient who was successfully treated by autologous bone marrow-derived stem ce...
Book
This book introduces many new technologies and clinical applications of hematopoietic stem cells and mesenchymal stem cell transplantation for the treatment of autoimmune diseases and inflammatory diseases. Presented in two parts, Part 1 focuses on stem cell therapies for autoimmune disease treatment; Part 2 focuses on stem cell therapies and their...
Chapter
Introduction: Since the 1980s, adipose-derived stem cells (ASCs) have become a powerful and potential source for stem cell-based therapy, regenerative medicine, and even drug delivery in cancer treatment. The development of off-the-shelf mesenchymal stem cells (MSCs), including ASCs, has rapidly advanced in recent years with several clinical trial...
Conference Paper
Cytokine-induced killer cells (CIK) are cytotoxic T cells, which have both NK and T cell properties. These cells are characterized by potent, non-MHC-restricted cytotoxicity and reduced alloreactivity, which make them appealing for use in adoptive immunotherapy of cancer and virus infections. In this study, CIK cells were generated by stimulating u...
Conference Paper
Breast cancer cells from patients hold an important role in antigen production for immunotherapy, drug testing, and cancer stem cell studies. To date, although many studies have been conducted to develop protocols for the isolation and culture of breast cancer cells from tumor biopsies, the efficiencies of these protocols remain low. This study aim...
Conference Paper
Recently, stem cell therapy has been investigated as a strategy to prevent or reverse damage to heart tissue. Although the results of cell transplantation in animal models and patients with myocardial ischemia are promising, the selection of the appropriate cell type remains an issue that requires consideration. In this study, we aimed to evaluate...
Conference Paper
Mesenchymal stem cells (MSCs) are now an attractive source of cell therapy. We test the hypothesis that autologous adipose-derived mesenchymal stem cells ameliorate liver cirrhosis in mice. In this study, male Swiss mice were treated orally with olive oil or CCl4 for 11 weeks (3 times/week). Mouse adipose-derived stem cells (mADSCs) from adipose ti...
Article
The Science and Technology Development Journal (STDJ) is the official scientific publication of the Viet Nam National University Ho Chi Minh City, Viet Nam (VNUHCM). The STDJ was firstly published in 1998 to aim push the publication activities of students, researchers, and scientists of VNUHCM. Since then, STDJ has become the most important scienti...
Chapter
Full-text available
Background: Cancer is one of the leading causes of death in the world. A great deal of effort has been made to discover new agents for cancer treatment. Xao tam phan (Paramignya trimera) is a traditional medicine of Vietnam used in cancer treatment for a long time, yet there is not much scientific evidence proving its anticancer potency. The study...
Article
Introduction: Adipose-derived stem cells (ADSCs) are considered as mesenchymal stem cells (MSCs). Indeed, they display all characteristics of MSCs that compliant with the minimal criteria of MSCs suggested by Domonici et al. (2006). However, some recent studies showed that ADSCs contain the subpopulation that was positive with CD34 marker – a marke...
Article
Full-text available
Introduction: Cartilage injury is the most common injury among orthopedic diseases. The predominant treatment for this condition is cartilage transplantation. Therefore, production of cartilage for treatment is an important strategy in regenerative medicine of cartilage to provide surgeons with an additional option for treatment of cartilage defect...
Article
Stem cell research and therapy are one of the most attractive studies in the biomedicine. Not only in the bench, nowadays stem cells but also become the bustling industry. In Vietnam, biomedical scientists started to study and apply stem cells since 1995. From that, Vietnamese scientists got some significant achievements in stem cell research and t...
Chapter
Exosomes as well as other extracellular vesicles (EVs) play an important role in cell–cell communication. Recently, EVs have been used as a platform for cellular therapy for conditions such as degenerative diseases. EVs were demonstrated to stimulate angiogenesis, cell proliferation, and immune modulation. This study aims to determine the factors,...
Chapter
Of all stem cells in the human body, mesenchymal stem cells (MSCs) are the most popular with regard to their potential and use in therapy. They can be easily identified and isolated from various tissues, and they can differentiate into a variety of mature cell types (bone, fat, cartilage, etc.). Unlike other adult stem cells, MSCs possess a variety...
Chapter
Stem cell transplantation has become the new therapy for various degenerative diseases (such as Parkinson’s disease and spinal cord injury), and even for myocardial infarction, type II diabetes mellitus, and cancer. From the first transplantation of therapeutic stem cells in humans to the present day, the use of stem cells or stem cell-based produc...
Chapter
Mesenchymal stem cells (MSCs) are common stem cells in the human body. They have various properties and functions, including migration to tumors. Based on this property, MSCs have been used as vectors to deliver anti-cancer agents to tumors. To date, MSCs have been modified to express some factors or agents, including interleukin (IL)-12, IL-14, IF...
Book
This invaluable resource discusses insights ranging from basic biological mechanisms of various types of stem cells through the potential applications in the treatment of human diseases, including cancer and genetic disorders. These discoveries are placed within the structural context of tissue and developmental biology in sections dealing with rec...
Chapter
According to World Health Organization, cardiovascular disease, especially ischemic heart disease, is one of the top ten killers today. Therefore, a growing number of new therapies are being studied for curing this disease from new drug developments to new advance stent creations. Although these methods have given patients more comfortable than pre...
Chapter
Liver cirrhosis is characterized by inflammation and necrosis induced by Kupffer cells and the activation of hepatic satellite cells. In recent years, stem cell therapy has become a promising therapy for treatment. Some in vitro studies and in vivo preclinical trials have demonstrated that mesenchymal stem cells (MSCs) may improve liver cirrhosis b...
Article
Full-text available
Introduction Cancer is a disease with a global burden and is a major and increasing threat to public health. The demand for new modalities to treat and prevent cancer is high. Given the toxic side effects of standard treatments, such as chemotherapy, there is greater research interest in naturally derived compounds due to their selective toxicity t...
Article
Stem cell transplantation has been used to treat some diseases with promising results. Stem cells stem cells can rescue injured tissues through various mechanisms, such as stimulation of growth factor secretion, activation of other/local stem cells, differentiation into specialized cells, and modulation of the immune system. However, stem cell tran...
Article
Introduction: Endothelial cells (ECs) or endothelial progenitor cells (EPCs) are essential cells for blood vascular regeneration and vascular tissue engineering. However, the source of EPCs are limited. Indeed, these cells only existence with low rate at some tissues such as bone marrow, umbilical cord blood and peripheral blood. This study aimed t...
Article
Diabetes mellitus is a lifelong disease which causes negative effects on patient life. As a result of the disease, patients suffer from high blood sugar and the inability to obtain energy effectively from food. Diabetes mellitus can lead to life-threatening complications such as blindness, cardiovascular disease and diabetic coma. Diabetes can aris...
Article
Introduction: Pathological animal models provide the foundation for developing new methods for disease treatment. This research aims to establish a rabbit model of femoral head necrosis. Methods: Osteonecrosis of the femoral head (ONFH) was induced in rabbits by using methylprednisolone (MPS) combined with Complete Freund's Adjuvant (CFA). New Zea...
Chapter
Autoimmune diseases (ADs) affect approximately 3% of the global population. Almost all AD patients experience negative effects after long-term treatment with immunosuppressive drugs. In recent years, stem cell therapy has become a promising treatment for these diseases. Two kinds of stem cells have been used in AD patients, hematopoietic stem cells...
Article
Background: Stem cell therapy in liver cirrhosis treatment is attracting the attention of the scientific community. Adipose tissue-derived mesenchymal stem cells are a potential source of cells because they have self-renewal, high proliferation, and differentiation into a variety of cell types, including hepatocytes as potential cell sources for ci...