Philippe Prior

Philippe Prior
French National Institute for Agriculture, Food, and Environment (INRAE) | INRAE · SPE/BIOS

PhD

About

239
Publications
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Publications

Publications (239)
Article
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Ralstonia solanacearum is a well-known agricultural and ecological threat worldwide. The complexity of the R. solanacearum species complex (Rssc) represents a challenge for the accurate characterization of epidemiological strains by official services and research laboratories. The majority of protocols only focus on a narrow range of strains; howev...
Article
Full-text available
The increased availability of genome sequences has advanced the development of genomic distance methods to describe bacterial diversity. Results of these fast-evolving methods are highly correlated with those of the historically standard DNA-DNA hybridization technique. However, these genomic-based methods can be done more rapidly and less expensiv...
Article
Full-text available
Ralstonia solanacearum, a species complex of bacterial plant pathogens divided into four monophyletic phylotypes, causes plant diseases in tropical climates around the world. Some strains exhibit a broad host range on solanaceous hosts, while others are highly host-specific as for example some banana-pathogenic strains. Previous studies showed that...
Article
Full-text available
The Ralstonia solanacearum species complex (Rssc) encompasses strains that are highly destructive on a wide host range, and some are listed as quarantine organisms in Europe, the United States, and many other countries. This soil-borne bacterial plant pathogen invades the roots and colonizes the xylem vessels, causing a bacterial wilt (BW) disease....
Article
Full-text available
Unlabelled: Plants use the signaling molecule salicylic acid (SA) to trigger defenses against diverse pathogens, including the bacterial wilt pathogen Ralstonia solanacearum SA can also inhibit microbial growth. Most sequenced strains of the heterogeneous R. solanacearum species complex can degrade SA via gentisic acid to pyruvate and fumarate. R....
Article
Full-text available
Reliable genotyping that provides an accurate description of diversity in the context of pathogen emergence is required for the establishment of strategies to improve disease management. MultiLocus variable number tandem repeat analysis (MLVA) is a valuable genotyping method. It can be performed at small evolutionary scales where high discriminator...
Article
Full-text available
Background. Ralstonia solanacearum is an economically important plant pathogen with an unusually large host range. The Moko (banana) and NPB (not pathogenic to banana) strain groups are closely related but are adapted to distinct hosts. Previous comparative genomics studies uncovered very few differences that could account for the host range differ...
Poster
Full-text available
Diversity in the South-West Indian Ocean. Surveys, phylotype analysis and phylogenetic tree by egl sequencing.
Article
Full-text available
Ralstonia solanacearum is a vascular soil-borne plant pathogen with an unusually broad host range. This economically destructive and globally distributed bacterium has thousands of distinct lineages within a heterogeneous and taxonomically disputed species complex. Some lineages include highly host-adapted strains (ecotypes), such as the banana Mok...
Article
Full-text available
Banana wilt outbreaks that are attributable to Moko disease-causing strains of the pathogen Ralstonia solanacearum (Rs) remain a social and economic burden for both multinational corporations and subsistence farmers. All known Moko strains belong to the phylotype II lineage, which has been previously recognized for its broad genetic basis. Moko str...
Article
Full-text available
The epidemic situation of Moko disease-causing strains in Latin America and Brazil is unclear. Thirty-seven Ralstonia solanacearum strains from Brazil that cause the Moko disease on banana and heliconia plants were sampled and phylogenetically typed using the endoglucanase (egl) and DNA repair (mutS) genes according to the phylotype and sequevar cl...
Conference Paper
Full-text available
Bacterial wilt, caused by Ralstonia solanacearum (Rs) (Smith) Yabuuchi et al., is one of the most important diseases of potato crops worldwide, including Madagascar. From the literature, strains of Rs phylotypes III or I were pathogenic to Solanaceae in the highlands potato areas (1,2). From years these strains were managed through the deployment o...
Article
Bacterial wilt caused by Ralstonia solanacearum is a destructive disease for many crops. The aim of this study is to investigate the phylogenetic relationships and genetic structure of a R. solanacearum population from diverse origins in Taiwan. All tested 58 strains belonged to phylotype I, except the two biovar 2 strains from potato. These belong...
Article
Full-text available
Unlabelled: During bacterial wilt of tomato, the plant pathogen Ralstonia solanacearum upregulates expression of popS, which encodes a type III-secreted effector in the AvrE family. PopS is a core effector present in all sequenced strains in the R. solanacearum species complex. The phylogeny of popS mirrors that of the species complex as a whole,...
Article
Full-text available
Ralstonia solanacearum causes bacterial wilt disease in many plant species, including mulberry. Here, we used a suppression subtractive hybridization (SSH) approach to identify specific DNA fragments in R. solanacearum race 5-biovar 5. The genome of the R. solanacearum M7 strain was subtracted from that of the GMI1000 strain, resulting in the ident...
Article
Full-text available
Because it is suspected that gene content may partly explain host adaptation and ecology of pathogenic bacteria, it is important to study factors affecting genome composition and its evolution. While recent genomic advances have revealed extremely large pan-genomes for some bacterial species, it remains difficult to predict to what extent gene pool...
Data
CGH microarrays validation on R229 and UW551. Plot of the false-positive (blue), and false-negative (blue) calls from the CGH-microarrays on the UW551 (solid lines) and R229 (dashed lines) genomes depending on the cutoff used to define homology. (B) Pairwise homology of the CDS from the six sequenced genomes targeted by the same probe. (TIFF)
Data
Phylogenetic tree based on the 2,992 blocks of probes. Phylogenetic tree of the R. solanacearum species complex inferred using MrBayes and based on the hybridization results of 2,992 blocks of contiguous probes in the genomes and display the same evolutionary patterns. Strains used for the construction of the microarrays are in bold. Phylotype clas...
Data
Cold-spots and hot-spots of gene movement. Schematic representation of the cold-spots and hot-spots of gene movement along the genomes of the GMI1000, CFBP2957, CMR15 and PSI07 strains. Cold-spots are indicated in blue while hot-spots are indicated in red. Putative IS elements are represented with purple lines. On every genome, two tests (p-value <...
Data
Distribution of the genes specific to the six sequences strains. Phylogenetic tree of the R. solanacearum species complex along with the per-strain proportion of genes targeted by probes designed as specific to the GMI1000, CFBP2957, Molk2, IPO1609, CMR15 and PSI07 strains. (TIFF)
Article
Full-text available
Textes issus des travaux du programme Casdar "Innovation et Partenariat" de 2007 et présentés lors d'un colloque le 4 décembre 2012, sous l'égide du GIS Relance Agronomique Article consultable sur le site : http://www6.inra.fr/ciag/Revue/Volumes-publies-en-2012/Volume-25-Decembre-2012
Article
Full-text available
The genetic diversity of Ralstonia solanacearum causing bacterial wilt of tomato in Trinidad was assessed using the hierarchical phylotyping scheme and rep‐PCR DNA fingerprinting. Seventy‐one isolates were collected in 2003 on infected tomato crops in the four main vegetable cropping areas of Trinidad (North, Central, South‐East and South). Two phy...
Article
Full-text available
Resistance of eggplant against Ralstonia solanacearum phylotype I strains was assessed in a F(6) population of recombinant inbred lines (RILs) derived from a intra-specific cross between S. melongena MM738 (susceptible) and AG91-25 (resistant). Resistance traits were determined as disease score, percentage of wilted plants, and stem-based bacterial...
Article
Full-text available
Ralstonia solanacearum is a widespread and destructive plant pathogen. We present the genome of the type strain, K60 (phylotype IIA, sequevar 7). Sequevar 7 strains cause ongoing tomato bacterial wilt outbreaks in the southeastern United States. K60 generally resembles R. solanacearum CFBP2957, a Caribbean tomato isolate, but has almost 360 unique...
Article
Full-text available
We used multilocus sequence analysis (MLSA) on a worldwide collection of the plant pathogenic Ralstonia solanacearum (Betaproteobacteria) to retrace its complex evolutionary history. Using genetic imprints left during R. solanacearum evolution, we were able to delineate distinct evolutionary complex displaying contrasting dynamics. Among the phylot...
Article
Full-text available
The ancient soilborne plant vascular pathogen Ralstonia solanacearum has evolved and adapted to cause severe damage in an unusually wide range of plants. In order to better describe and understand these adaptations, strains with very similar lifestyles and host specializations are grouped into ecotypes. We used comparative genomic hybridization (CG...
Article
Full-text available
In March 2007, severe symptoms of leaf curling and yellowing resembling tomato yellow leaf curl disease were observed on tomato ( Solanum lycopersicum ) plants with a very high incidence in six sites on Grenada Island (Fig. 1; Table 1).…
Article
Full-text available
We used multilocus sequence analysis (MLSA) on a worldwide collection of the plant pathogenic Ralstonia solanacearum (Betaproteobacteria) to retrace its complex evolutionary history. Using genetic imprints left during R. solanacearum evolution, we were able to delineate distinct evolutionary complex displaying contrasting dynamics. Among the phylot...
Data
Overview of genomes in the R. solanacearum species complex. (XLS)
Data
A: percentage of CDS in synteny and average number of CDS per synteny with PSI07 as reference strain. B: PSI07 chromosome and megaplasmid alignment with the complete genome of BDB and R. syzygii. (XLS)
Data
R. solanacearum virulence genes (updated from Remenant et al [3] ). (XLS)
Data
Gene expression patterns of specific functional clusters in GMI1000 and UW551 in planta vs. in rich medium, and their presence in Phylotype IV strains PSI07, BDB, and R. syzygii. (XLS)
Data
List of adhesins and hemagluttinins genes in sequenced strains from the R. solanacearum species complex. (XLS)
Article
Full-text available
The Ralstonia solanacearum species complex includes R. solanacearum, R. syzygii, and the Blood Disease Bacterium (BDB). All colonize plant xylem vessels and cause wilt diseases, but with significant biological differences. R. solanacearum is a soilborne bacterium that infects the roots of a broad range of plants. R. syzygii causes Sumatra disease o...
Article
Full-text available
Ralstonia solanacearum strain Po82, a phylotype IIB/sequevar 4 strain, was found to be pathogenic to both solanaceous plants and banana. Here, we report the complete genome sequence of Po82 and its comparison with seven published R. solanacearum genomes.
Article
Bacterial wilt caused by Ralstonia solanacearum is a serious threat to crop production in China. A collection of 319 R. solanacearum strains isolated from 14 different diseased host plants collected in 15 Chinese provinces was investigated by BOX fingerprints in order to test the influence of the site and the host plant on their genetic diversity....
Article
Full-text available
Resistance of eggplant against Ralstonia solanacearum phylotype I strains was assessed in a F 6 population of recombinant inbred lines (RILs) derived from a intra-specific cross between S. melongena MM738 (susceptible) and AG91-25 (resistant). Resistance traits were determined as disease score, percentage of wilted plants, and stem-based bacterial...
Article
Full-text available
Bacterial wilt, caused by strains belonging to the Ralstonia solanacearum species complex, inflicts severe economic losses in many crops worldwide. Host resistance remains the most effective control strategy against this disease. However, wilt resistance is often overcome due to the considerable variation among pathogen strains. To help breeders ci...
Article
Full-text available
Horizontal gene transfer (HGT) is a major driving force of evolution and is also likely to play an important role in the threatening emergence of novel pathogens, especially if it involves distantly related strains with substantially different pathogenicity. In this study, the impact of natural transformation on pathogenicity in six strains belongi...
Article
Full-text available
Based on the phylotype classification, we questioned how genetically and phenotypically diverse strains of Ralstonia solanacearum pathogenic to potato may be. We studied 129 European and Mediterranean strains along with 57 reference strains known to cover genetic diversity in this species. Phylogeny analysis was done on endoglucanase gene sequences...
Article
Full-text available
Bacterial spot of tomato and pepper (BSTP) can be caused by several #Xanthomonas genospecies#. BSTP is a major disease in Grenada where A and B phenotypic groups (#Xanthomonas euvesicatoria# and #X. vesicatoria#, respectively, [2]) have been reported. There is no previous report of group A strains, which are strongly amylolytic and pectolytic, in G...