Philippe Michaud

Philippe Michaud
Université Clermont Auvergne | Univ BPC · Clermont Auvergne INP - Institut Pascal

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About

354
Publications
132,483
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Introduction
Philippe Michaud currently works at the Institut Pascal, Université Clermont Auvergne. Philippe does research in Biochemistry, and biological engineering
Additional affiliations
September 2005 - present
Université Clermont Auvergne
Position
  • Professor
Description
  • The axis Chemical Engineering, Applied Thermodynamic and Biosystems of the Institut Pascal focus on Chemical, Biological and Food Engineering.
September 1997 - August 2005
Université de Picardie Jules Verne
Position
  • Professor (Assistant)
Description
  • Biochemistry of carbohydrate and more especially of poly- and oligosaccharides

Publications

Publications (354)
Article
Microalgae are photoautotrophic microorganisms known as producers of a large variety of metabolites. The taxonomic diversity of these microorganisms has been poorly explored. In this study, a newly isolated strain was identified based on the 18S rRNA encoding gene. The phylogenetic analysis showed that the isolated strain was affiliated with the Rh...
Article
Full-text available
The brown Tunisian seaweed Halopteris scoparia was used as a feedstock for producing renewable bioethanol, biogas, and biodiesel to demonstrate the proof of concept for the North African energy sector. A quantitative and qualitative quantification of H. scoparia composition using different colorimetric methods was completed to highlight its bioconv...
Article
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Lignocellulose, the main component of plant cell walls, comprises polyaromatic lignin and fermentable materials, cellulose and hemicellulose. It is a plentiful and renewable feedstock for chemicals and energy. It can serve as a raw material for the production of various value-added products, including cellulase and xylanase. Cellulase is essential...
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Methods for purifying, detecting, and characterizing protein concentrate, carbohydrates, lipids, and neutral fats from the microalgae were developed as a result of research. Microalgae were collected from natural sources (water, sand, soil of the Kaliningrad region, Russia). Microalgae were identified based on morphology and polymerase chain reacti...
Article
Given the increasing interest that is being paid to polysaccharides derived from algae as plant natural defense stimulators, the degree of sulfation of exopolysaccharides produced by P. sordidum for inducing defense responses in date palm vitroplants was investigated. Firstly, the culture parameters of P. sordidum were optimized to maximize the amo...
Article
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Alginate is a hydrocolloid from algae, specifically brown algae, which is a group that includes many of the seaweeds, like kelps and an extracellular polymer of some bacteria. Sodium alginate is one of the best-known members of the hydrogel group. The hydrogel is a water-swollen and cross-linked polymeric network produced by the simple reaction of...
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The development of eco-friendly cosmetic such as those from microalgae for skin regeneration and collagen synthesis has gained a great interest worldwide. Accordingly, the potential of microalgae biomass as source of anti-aging cosmetic cream with high antioxidant activity has been investigated. Stabilities and sensory characteristics of cosmetic c...
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Sulfated glycoproteins extracted for the first time from the Moroccan green seaweed Codium decorticatum were investigated for their ability to induce a natural defense metabolism in the roots and the upper leaves of tomato seedlings. The crude (AGB) and the purified fractions (AGP) were characterized chemically (Colorimetric assays) and structurall...
Article
The coronavirus pandemic (COVID-19) has created an urgent need to develop effective strategies for prevention and treatment. In this context, therapies against protease Mpro, a conserved viral target, would be essential to contain the spread of the virus and reduce mortality. Using combined techniques of structure modelling, in silico docking and p...
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The influence of soaking and germination on the physicochemical characteristics of tigernut tubers (Cyperus esculentus L.) are investigated. Tubers (Ø > 1 cm) were soaked in an ascorbic acid solution (0.1%) for 48 h at 40 °C before undergoing germination for 6 days. The textural profile, the capacity and germination energy and the biochemical compo...
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The beneficial properties of algae make them perfect functional ingredients for food products. Algae have a high energy value and are a source of biologically active substances, proteins, fats, carbohydrates, vitamins, and macro- and microelements. They are also rich in polyunsaturated fatty acids, proteins, mycosporine-like amino acids, polysaccha...
Article
Lignocellulosic by-products are frequently disposed by means of combustion. This study investigates an alternative route for corn cob and Kraft lignin resources in order to support circular economy. The respective plant-based fibres and filler were compounded for the first time together with a poly(lactic acid) (PLA) matrix. Consecutively, seven di...
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In the current context of the COVID-19 pandemic, it appears that our scientific resources and the medical community are not sufficiently developed to combat rapid viral spread all over the world. A number of viruses causing epidemics have already disseminated across the world in the last few years, such as the dengue or chinkungunya virus, the Ebol...
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Nowadays, microalgae and cyanobacteria have become a promising and sustainable source of useful products, thanks to their richness in bioactive metabolites of high value (antibiotics, toxins, pharmaceutically active compounds, plant growth regulators, and others). These photoautotroph microorganisms generate biomass using photosynthesis. This revie...
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Recent years, algal polysaccharides were mainly investigated for pharmaceutical, nutraceuticals and biomedical applications due to their promising bioactive properties. Moreover, it is now becoming a potential source for several non-medical and non-food applications in which biofuels are a major sector. The marine algae contain a variety of polysac...
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The COVID-19 new variants spread rapidly all over the world, and until now scientists strive to find virus-specific antivirals for its treatment. The main protease of SARS-CoV-2 (Mpro) exhibits high structural and sequence homology to main protease of SARS-CoV (93.23% sequence identity), and their sequence alignment indicated 12 mutated/variant res...
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In many African countries, the Bayoud is a common disease spread involving the fungus Fusarium oxusporum f. sp. albedinis (Foa). The induction of plant natural defenses through the use of seaweed polysaccharides to help plants against pathogens is currently a biological and ecological approach that is gaining more and more importance. In the presen...
Chapter
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Glucuronan and succinoglycan are two anionic exopoysaccharides produced by some soil bacteria and abundantly published by the community working on high valuable hydrocolloids. The glucuronan is a linear homopolymer of β-(1,4)-D�glucuronic acids sometimes subsituted by O-acetyl, whereas succinoglycan is ramified heteropolymer of β-(1,6) and β-(1,4)...
Chapter
As a general rule, in a sustainable food model for the future of the growing world population, food biotechnology could be considered as a reliable for improving and increasing food production and processing. Food biotechnology consist of (1) genetic engineering approach (on plants, animals, or microorganisms) to improve food quality, (2) commercia...
Article
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The anti-inflammatory and antihyperglycemic effects of polysaccharides extracted from Alhagi maurorum Medik. seeds, spontaneous shrub collected in Southern of Algerian Sahara were investigated. Their water extraction followed by alcoholic precipitation was conducted to obtain two water-soluble polysaccharides extracts (WSPAM1 and WSPAM2). They were...
Article
Alhagi maurorum Medik. (Fabaceae) is a spontaneous shrub native to the region extending from Russia to the Mediterranean and used by rural and Saharan Algerian people for its medicinal effects. This study aims to document the medicinal uses of A. maurorum in Illizi (southern Algeria). Ethnobotanical survey was carried out in Illizi between June and...
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Polysaccharides are complex macromolecules long regarded as energetic storage resources or as components of plant and fungal cell walls. They have also been described as plant mucilages or microbial exopolysaccharides. The development of glycosciences has led to a partial and difficult deciphering of their other biological functions in living organ...
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Chitosan is a bio-sourced polysaccharide widely used in different fields from health to wastewater treatment through food supplements. Another important use of this polymer is adhesion. Indeed, the current demand to replace non-natural and hazardous polymers by greener ones is well present in the adhesive field and open good opportunities for chito...
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In the last 20 years, xanthophylls from microalgae have gained increased scientific and industrial interests. This review highlights the essential issues that concern this class of high value compounds. Firstly, their chemical diversity as the producer microorganisms was detailed. Then, the use of conventional and innovative extraction techniques w...
Article
This work was initiated to improve the thermophysical quality of biomass pellets produced from rice husks using arabic gum. Dried and crushed arabic gum was solubilized in a 1:2, 1:3 and 1:4 m/v binder/water ratios. In addition, the rice husks and pellets were physiochemically characterized. The formulation of the mixture was carried out by mixing...
Article
Microsporidia are obligate intracellular pathogens capable of infecting a wide variety of hosts ranging from invertebrates to vertebrates. The infection process requires a step of prior adherence of Microsporidia to the surface of host cells. A few studies demonstrated the involvement of proteins containing a ricin-B lectin (RBL) domain in parasite...
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Amylases are enzymes required for starch degradation and are naturally produced by many microorganisms. These enzymes are used in several fields such as food processing, beverage, and medicine as well as in the formulation of enzymatic detergents proving their significance in modern biotechnology. In this study, a three-stage growth mode was applie...
Article
The physical functions of extracellular polymeric substances (EPS), viz., by-product of microphytobenthos (MPB), in tidal flat system are well documented, but some ecological aspects remain unknown. We investigated MPB biomass (Chl-a), EPS, diatom assemblage, and erodibility in two contrasting tidal flat environments (megatidal vs. macrotidal flat)...
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In this present work, we developed a phenol grafted polyglucuronic acid (PGU) and investigated the usefulness in tissue engineering field by using this derivative as a bioink component allowing gelation in extrusion-based 3D bioprinting. The PGU derivative was obtained by conjugating with tyramine, and the aqueous solution of the derivative was cur...
Article
Sulfated exopolysaccharides (EPS) from Porphyridium cruentum strain were extracted and their antioxidant and anti-bacterial potentials were evaluated based on DPPH free radical, ABTS•+ radical cation and DNA nicking assays, and against four foodborne pathogenic bacteria, respectively. They showed also interesting functional, foaming and emulsion pr...
Article
Cette étude porte sur la caractérisation structurale partielle et les activités biologiques d'une fraction polysaccharidique (GFC) isolée, par macération à chaud, de la gomme-résine de Ferula communis L. (Apiaceae) récoltée au Sahara. Le rendement massique de l'extrait était de 33,5 %. L'étude de la composition biochimique de cet extrait a révélé q...
Article
The purpose of this study is to improve microbiological qualities of black tiger nut tubers. Thereby, black variety of tiger nut tubers (Cyperus esculentus) were differently processed in ascorbic acid and alkaline (Ca(OH)2 and Kanwa (alkaline calcium carbonate locally used to soften vegetable)) solutions at different concentrations and temperatures...
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The global energy trends are currently dominated by a massive use of fossil non-renewable energy sources which are progressively depleting. In this way, the production of second-generation biogas production from organic wastes by the dark fermentation process offers, therefore, an attractive solution to diversify the present energy mix. The develop...
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Enzymatic hydrolysis of cellulose-containing raw materials, using Aspergillus niger, were studied. Filter paper, secondary cellulose-containing or starch-containing raw materials, miscanthus cellulose after alkaline or acid pretreatment, and wood chip cellulose, were used as substrates. The study focused on a wild A. niger strain, treated, or not (...
Article
Metal elements are widely used in various industrial activities and are considered common water source contaminants. Thus, the development of cost-effective, simple design and efficient processes for trace metal elements removal from contaminated water sources is of great interest. The effects of cadmium, lead and chromium on growth, biomolecules a...
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Currently, pectins are widely used in the cosmetic, pharmaceutical, and food industries, mainly as texturizing, emulsifying, stabilizing, and gelling agents. Pectins are polysaccharides composed of a large linear segment of α-(1,4) linked d-galactopyranosyluronic acids interrupted by β-(1,2)-linked l-rhamnoses and ramified by short chains composed...
Chapter
One of the most important challenges in drug delivery is the permeability of drugs as carriers to target cells. Depending on the medication interaction with target cell cause efficiency and high uptake of them. Hence drug loading happens in each cell of the body without the various side effects of the drugs. One example of various side effects of t...
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Our study focused on investigating the possibilities of controlling the accumulation of carbohydrates in certain microalgae species (Arthrospira platensis Gomont, Chlorella vulgaris Beijer, and Dunaliella salina Teod) to determine their potential in biofuel production (biohydrogen). It was found that after the introduction of carbohydrates (0.05 g⋅...
Article
The oil cake from argan, an available raw material in Morocco, was explored for biodiesel production. The residual lipids of roasted and unroasted argan cakes were transesterified under alkaline conditions. The best biodiesel yield (94%) was obtained after transesterification of residual lipids from roasted argan cake using a methanol:lipid ratio o...
Article
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Microalgae are attracting considerable interest worldwide. In the present study, two native microalgae strains isolated from Tunisian cost were identified as Chlamydomonas sp. and Navicula sp. We characterized their pigment and protein contents, as well as their carbohydrate and lipid productivity. The predominant fatty acids were found to be α-lin...
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Polysaccharides are highly variable and complex biomolecules whose inventory of structures is still very incomplete, as nature still preserves unexplored biotopes. Plants, macroalgae and microalgae are an integral part of the daily life of human being regardless of culture, time, or knowledge development of a country. Natural medicine is an ancestr...
Article
Alginic acids and alginates have important applications in food, medicine, pharmaceutical industry, dentistry and textile. The sodium alginate from Nizimuddinia zanardini (an Iranian brown seaweed) was extracted with high voltage electrical discharge to investigate the influence of this extraction method on its structural and physico-chemical chara...
Article
Nowadays, the use of biostimulants in agriculture has become an innovative tool to cope with several environmental constraints. Seaweed extracts represent an interesting category of biostimulants which are gaining traction as agricultural applications. The present work aims at highlighting the effect of the aqueous extracts of the brown macroalgae...
Patent
The present invention relates generally to polymeric compounds of glucuronic acid and a method of hydrogelation of such compounds. The present invention also relates to the use of the hydrogel structures (particles, films or 3D-structures) obtained by this method, notably as three-dimensional cell culture material to support biological molecules us...
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Today, an ethnobotanical approach makes sense for identifying new active bioactive chemicals from uses of indigenous plants. Two water-soluble enriched polysaccharide fractions (L-PSPN and S-PSPN) were extracted by hot water extraction from the leaves and seeds of Plantago ciliata Desf. (1798), a Mzab indigenous herb currently used in Algeria by tr...
Article
Arabinogalactans (AGs) are plant heteropolysaccharides with complex structures occasionally attached to proteins (AGPs). AGs in cell matrix of different parts of plant are freely available or chemically bound to pectin rhamnogalactan. Type I with predominantly β-d-(1 → 4)-galactan and type II with β-d-(1 → 3) and/or (1 → 6)-galactan structural back...
Article
Tetraselmis sp. strain MD-01, a microalga isolated from the Tunisian marine coast, has been studied for autotrophic lipids production with two stage process. This strategy was cultured for maximum cells production under moderated conditions (2.12 g.L⁻¹), followed by cultivation under stressed conditions to induce lipid synthesis. High irradiance (3...
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Argan pulp is an abundant byproduct from the argan oil process. It was investigated to study the feasibility of second-generation bioethanol production using, for the first time, enzymatic hydrolysis pretreatment. Argan pulp was subjected to an industrial grinding process before enzymatic hydrolysis using Viscozyme L and Celluclast 1.5 L, followed...
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Commercial adhesives present a high bond strength and water resistance, but they are considered non-healthier products. Chitosan can be considered as an interesting biosourced and biodegradable alternative, despite its low water resistance. Here, its wood bonding implementation and its tensile shear strength in dry and wet conditions were investiga...
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Polysaccharides from marine algae are one novel source of plant defense elicitors for alternative and eco-friendly plant protection against phytopathogens. The effect of exopolysaccharides (EPS) produced by Porphyridium sordidum on elicitation of Arabidopsis thaliana defense responses against Fusarium oxysporum was evaluated. Firstly, in order to e...
Article
Phycoremediation is being considered as an eco-friendly and safe technology for toxics eradication from contaminated aquatic systems. The zinc biosorption capacity of Dunaliella sp. AL-1 was demonstrated. Zinc impacted cell growth and photosynthetic pigments accumulation showing exposure time and concentration-dependent effects. The investigation o...
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Microalgae have been poorly investigated for new-lipolytic enzymes of biotechnological interest. In silico study combining analysis of sequences homologies and bioinformatic tools allowed the identification and preliminary characterization of 14 putative lipases expressed by Chlorella vulagaris. These proteins have different molecular weights, subc...
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Fucoidans from Moroccan brown seaweed Bifurcaria bifurcata and Fucus spiralis were tested for their elicitor activity after their purification and complete characterization. The fucoidans of B. bifurcata (BBF) and of F. spiralis (FSF) were extracted and purified then characterized by infrared spectroscopy, proton nuclear magnetic resonance spectros...
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In the last decades, many studies have been conducted on new materials to meet a growing industrial demand and to move scientific research forward. Superabsorbents are good examples of materials that have generated special attention in many fields for their ability to absorb and retain water up to 1000 times of their dry weight. They found many app...
Article
Proteases are industrially important catalysts. They belong to a complex family of enzymes that perform highly focused proteolysis functions. Given their potential use, there has been renewed interest in the discovery of proteases with novel properties and a constant thrust to optimize the enzyme production. In the present study, a novel extracellu...