Philip Fortgens

Philip Fortgens
Groote Schuur Hospital · Department of Chemical Pathology

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10
Publications
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261
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Publications

Publications (10)
Article
Point-of-care testing is becoming common practice in a variety of settings, including extreme environments. Little is known about the functioning of these platforms under challenging conditions. The i-STAT (Abbott, Princeton, NJ) is a cartridge-based point-of-care system for the testing of whole blood. This pilot study compares the performance of i...
Article
Aims Acute intermittent porphyria (AIP) is a disorder of the haem biosynthetic pathway caused by mutations in the hydroxymethylbilane synthase (HMBS) gene. Knowledge of the spectrum of mutations present in South Africa is limited. This study presents the molecular profile of 20 South African patients with AIP, and the kinetic analysis of one novel...
Article
Full-text available
Background Immunoassays are prone to interference by various substances which may cause inaccurate results. This type of interference is difficult to detect analytically. Objective A case of CARDIAC Troponin T Quantitative reader (Roche Diagnostics) assay failure was detected and investigated in order to ascertain the likely cause. Method Patient...
Article
Factitiously low sodium estimations are a hazard in most modern clinical laboratories. Most modern high-throughput analyzers use indirect ion-selective electrodes to estimate electrolyte concentrations in serum samples. This analysis is preceded by a dilution step of the sample. If the water concentration is altered by the presence of increased lip...
Article
Factitiously low sodium estimations are a hazard in most modern clinical laboratories. Most modern high-throughput analyzers use indirect ion-selective electrodes to estimate electrolyte concentrations in serum samples. This analysis is preceded by a dilution step of the sample. If the water concentration is altered by the presence of increased lip...
Article
Hyperexpression, alteration of trafficking and secretion of cathepsin D has been linked with tumour invasion and inflammation. To study these phenomena in a variety of cells large quantities of anti-cathepsin D antibodies and the appropriate immunogen are required. As the human immunogen for studies on human tissue is less easily accessed, antibodi...
Article
Full-text available
Paraformaldehyde (PFA) fixation was optimized to facilitate the immobilization and labeling of multiple granule antigens, using short fixation regimens and cryoultramicrotomy of unembedded neutrophils (PMNs). In the optimal protocol, extraction of azurophil granule antigens (especially of the abundant elastase) was obviated by manipulating the poly...
Article
Alterations in trafficking of cathepsins B and D have been reported in human and animal tumors. In MCF10 human breast epithelial cells, altered trafficking of cathepsin B occurs during their progression from a preneoplastic to neoplastic state. We now show that this is also the case for altered trafficking of cathepsin D. Nevertheless, the two cath...
Article
Anti-peptide antibodies were raised against synthetic peptides selected from the sequences of human cathepsins B and L, porcine cathepsin D and human type IV collagenase. Sequences were selected from the active site clefts of the cathepsins in the expectation that these would elicit immunoinhibitory antibodies. In the case of type IV collagenase a...

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