Peyman Norouzi

Sugar Beet Seed Institute · Department of Biotechnology

Agrobacterium rhizogenes-mediated transformation of sugar beet hairy roots expressing single-chain variable fragment (scFv) was exploited to evaluate the efficacy of four antibody-based constructs for interfering with the Beet necrotic yellow vein virus infection. The scFv specific to a major coat protein of virus, p21, was targeted to various cell...

Rhizomania is one of the most damaging sugar beet diseases and is caused by Beet necrotic yellow vein virus (BNYVV). In order to interfere with viral propagation, the expression of recombinant antibody strategy was employed. The coding sequence of a single-chain variable fragment (scFv) specific to the main BNYVV coat protein, P21, was targeted to...

Numerous diseases caused by fungal pathogens influence the annual production of sugar beet. In order to obtain a plant resistant to fungi, the genetic transformation has been applied to the sugar beet. To invade a plant tissue, phytopathogenic fungi produce several cell wall-degrading enzymes (CWDEs); Polygalacturonases (PGs) are pathogenicity fact...

Rhizomania is one of the most damaging diseases widely spread in major sugar beet growing regions of the world. The causal agent, beet necrotic yellow vein virus (BNYVV), is transmitted via Polymyxa betae fungus which retains it in the field for years. Here, we employed the RNA silencing mechanism to induce resistance against rhizomania using intro...

Plants are often exposed to abiotic stress such as drought, salinity, high or low temperature, deficiency of soil mineral nutrients and excessive or insufficient light. Plant reactions to stress occur at different scales, ranging from altered gene expression and cellular biochemistry to changed growth rate and yield. Nowadays, some reactions that a...

Sugar beet (Beta vulgaris L.) is an important industrial crop, the yield of which is strongly affected by numerous diseases caused by fungal pathogens. To the aim of developing transgenic plants resistant to fungi, two transgenic diploid sugar beet genotypes expressing the gene encoding the polygalacturonase inhibiting protein 2 of Phaseolus vulgar...

Rhizomania, caused by Beet necrotic yellow vein virus (BNYVV), is the most economically important diseases of sugar beet in Iran. We have identified molecular markers associated with resistance gene(s) to this disease. A F2 population including of 106 individuals developed at the Sugar Beet Seed Institute, Karaj, Iran, was used to identify molecula...

Two transgenic sugar beet T 0 lines, 7233-3 and 7233-8, carrying cry1Ab gene were selfed and crossed with a single cross cytoplasmic male sterile line. One hundred and ninety F 1 plants derived from the transgenic lines were screened using polymerase chain reaction analysis. Statistical test confirmed the 1: 1 Mendelian ratio for the transgene in F...

Monogermity is a character that is controlled by one pair of recessive allele in sugar beet. This character is used in many breeding projects. If the character is tagged with molecular markers, it will be possible to easily distinguish monogerm and multigerm plants by DNA extraction followed by PCR analysis in seedling stage. Bulked segregant analy...

Two diploid sugar beet genotypes of agronomical importance were transformed using Agrobactriumtumefaciens harboring pBI35Scry containing a synthetic cry1Ab gene. Leaf blade with attached shoot bases, a highly regenerative tissue, were used as explant substratum for transformation. PCR screening with cry1Ab-specific primers showed the presence of tr...

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RAPD markers linked to beet necrotic yellow vein virus (BNYVV) resistance genes were identified in two Beta vulgaris accessions Holly-1-4 and WB42 using bulked segregant analysis. The polymorphism revealed by the RAPD markers in the F2 generations of WB42 was higher than that of Holly-1-4. The segregation distortion at marker loci was slightly lowe...

In this study, sugar beet tissue culture clones were used to screen rhizomania resistant genotypes. At first, explants derived from shoot tips of sugar beet seedlings were transferred to shoot tip elongation media after surface sterilization. Then, the grown shoots were transferred to media containing various hormonal combinations NAA, BA, IBA and...

In this research, after optimization of sterilizing cyst and larvae of second stage of Heterodera schachtii, the possibility of using nematode on seedlings of sugar beet (Beta vulgaris L.) in in vitro condition was studied for developing larvae to cyst. For this purpose, non sterile cysts were extracted from infected soil and hatched into zinc chlo...

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Despite intensive efforts, a reproducible and reliable method for transformation of sugarbeet plants is still lacking. Having examined several explants, we found that cells around the main vein of leaves of plantlets reared from tissue-cultured apical meristems are sufficiently competent for transformation and subsequent regeneration. A transformat...

OF2 and VAP genes, probably involved in signal transduction of sugarbeet nematode resistance, have already been cloned in bacterial vector by AFLP molecular marker and a two-hybrid system, respectively. To examine their capability to introduce resistance in sugarbeet, the genes were transferred to plant expression vectors. For this reason, OF2 gene...