Petra De Sutter

Petra De Sutter
Ghent University | UGhent · Department of Uro-gynaecology

About

420
Publications
79,807
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
12,675
Citations
Citations since 2016
61 Research Items
6398 Citations
201620172018201920202021202202004006008001,000
201620172018201920202021202202004006008001,000
201620172018201920202021202202004006008001,000
201620172018201920202021202202004006008001,000

Publications

Publications (420)
Article
Human germline gene correction by CRISPR/Cas9 holds great promise to eliminate transmission of genetic mutations. Recent studies have however made troublesome observations of mosaicism (the occurrence of multiple genetic events in one embryo) and loss-of-heterozygosity (LOH, loss of the allele of one of the parents), the latter being either caused...
Article
Full-text available
Background Advanced maternal age and obesity are associated with impaired female fertility. Moreover, fatty acids (FA) in follicular fluid (FF) play important roles in oocyte maturation and embryo development. However, the effects of body mass index (BMI), age, and FF FA composition on embryo development between days 3 and 5 and blastocyst stage on...
Article
Full-text available
STUDY QUESTION Is the cumulative live birth rate (CLBR) per oocyte collection cycle (OCC) comparable after cleavage-stage or blastocyst-stage transfer in combination with supernumerary blastocyst vitrification on day 5 (D5) in patients with four or fewer zygotes on day 1? SUMMARY ANSWER The CLBR in a fresh blastocyst-transfer or cleavage-stage tra...
Article
Full-text available
Purpose Providing additional insights on the efficacy of human nuclear transfer (NT). Here, and earlier, NT has been applied to minimize transmission risk of mitochondrial DNA (mtDNA) diseases. NT has also been proposed for treating infertility, but it is still unclear which infertility indications would benefit. In this work, we therefore addition...
Article
Full-text available
The second trimester of human development is marked by asynchronous gonadal development hampering the isolation of homogenous populations of early and late fetal germ cells (FGCs). We evaluated the feasibility of using surface markers TNAP, PDPN, EPCAM and ITGA6 to isolate FGCs as well as human primordial germ cell-like cells (hPGCLCs) derived from...
Article
Objective To study the feasibility of in vitro maturation of ovarian tissue oocytes for fertility preservation in transgender men on testosterone treatment. Design Cross-sectional study Setting University hospital Patient(s) Eighty-three transgender men enrolled from November 2015 to January 2019 Intervention(s) In vitro maturation of cumulus-o...
Article
Full-text available
Protocols for specifying human primordial germ cell-like cells (hPGCLCs) from human embryonic stem cells (hESCs) remain hindered by differences between hESC lines, their derivation methods, and maintenance culture conditions. This poses significant challenges for establishing reproducible in vitro models of human gametogenesis. Here, we investigate...
Article
Background Up to 2018, the Belgian law stated that transgender people who wanted to change their legal sex had to undergo physical gender affirming treatment. This included gonadectomy to a medically possible and justified extent, which entailed that they had to accept the fact that they could no longer reproduce. However, research has shown that m...
Article
Mammalian fertilization encompasses a series of Ca2+ oscillations initiated by the sperm factor phospholipase C zeta (PLCζ). Some studies have shown that altering the Ca2+ oscillatory regime at fertilization affects pre-implantation blastocyst development. However, assisted oocyte activation (AOA) protocols can induce oocyte activation in a manner...
Chapter
The wish for children is the same in many transgender people as it is in the general population. However, a major difference is that unlike the general population, most transgender people require medical assisted reproduction (MAR) to achieve parenthood. The type of treatment offered to transgender people depends on many factors: if they wish to co...
Article
Study Question Is the implantation impaired in patients with endometriosis in In Vitro Fertilization/Intracytoplasmatic Sperm Injection (IVF/ICSI) cycles? Design A retrospective matched cohort study was executed on IVF/ICSI cycles with fresh single embryo transfer (SET) at the department of Assisted Reproductive Medicine at Ghent University Hospit...
Article
Full-text available
Recent progress has enabled the conversion of primed human embryonic stem cells (hESCs) to the naive state of pluripotency, resembling the well-characterized naive mouse ESCs (mESCs). However, a thorough histone epigenetic characterization of this conversion process is currently lacking, while its likeness to the mouse model has not been clearly es...
Article
Research question: Is there a difference in blastocyst formation between fresh and vitrified-warmed sibling oocytes and can this difference be attributed to changes in embryo morphokinetics? Design: Between February 2016 and December 2017, 472 metaphase II (MII) oocytes in 67 donor-recipient cycles from 27 different healthy anonymous oocyte dono...
Article
Full-text available
Our current knowledge of the mechanisms leading to human primordial germ cell (PGC) specification stems solely from differentiation experiments starting from human pluripotent stem cells. However, information regarding the origin of PGCs in vivo remains obscure. Here, we apply an improved system for extended in vitro culture of human embryos to inv...
Article
Objective: To investigate the extent to which assisted oocyte activation (AOA) improves clinical outcomes in patients diagnosed with oocyte activation deficiencies (OADs). Design: Retrospective cohort study comparing AOA cycles and previous intracytoplasmic sperm injection (ICSI) cycles in couples experiencing low or total failed fertilization a...
Article
Introduction: A lot of attention has been given to the quest of parents, children and donors to find donor siblings (= half siblings who share the same donor gametes but who are born in different families). However, literature is scarce about the use of the same sperm donor for subsequent children in the same family. Methods: This study included 68...
Article
Full-text available
Recently, secreted microRNAs (miRNAs) have received a lot of attention since they may act as autocrine factors. However, how secreted miRNAs influence embryonic development is still poorly understood. We identified 294 miRNAs, 114 known, and 180 novel, in the conditioned medium of individually cultured bovine embryos. Of these miRNAs, miR-30c and m...
Article
Abstract STUDY QUESTION What is the accuracy of preimplantation genetic testing for aneuploidies (PGT-A) when considering human peri-implantation outcomes in vitro? STUDY ANSWER The probability of accurately diagnosing an embryo as abnormal was 100%, while the proportion of euploid embryos classified as clinically suitable was 61.9%, yet if struct...
Article
The way in which heterosexual couples manage information about infertility and donor insemination within their social networks has not yet been explored in-depth. This study focuses on how parents and aspiring parents manage information about infertility and donor insemination within their social networks. Fifteen Belgian couples were interviewed a...
Article
Full-text available
Human embryonic stem cells (hESCs) hold great value for future clinical applications. However, standard culture conditions maintain hESCs in a primed state, which bears heterogeneity in pluripotency and a tendency for spontaneous differentiation. To counter these drawbacks, primed hESCs have been converted to a naive state, but this has restricted...
Article
Objective: To develop a random forest model (RFM) to predict implantation potential of a transferred embryo and compare it with a multivariate logistic regression model (MvLRM), based on data from a large cohort including in vitro fertilization (IVF) patients treated with the use of single-embryo transfer (SET) of blastocyst-stage embryos. Design:...
Article
Research question: Are there proteomic differences between endometrial stromal cells of repeated implantation failure (RIF), recurrent pregnancy loss (RPL) and normal fertile women, and is there differential protein expression upon decidualization? Design: This exploratory study investigated the proteome of in-vitro cultured endometrial stromal...
Thesis
Full-text available
BACKGROUND: Improving success rates of in vitro fertilization (IVF) and intra-cytoplasmic sperm injection (ICSI) has caused a rise in multiple pregnancy rates after multiple embryo transfer. This has led to a rise of the use of elective single embryo transfer (eSET), resulting in a higher need for choosing the best embryo for transfer in order to a...
Article
Background: Freezing all embryos generated during an IVF/ICSI attempt is used increasingly as a strategy to optimize results. We investigated whether we could find differences in outcome between subpopulations of patients undergoing the so-called "freeze all" procedure. Methods: Non-interventional, observational, retrospective study of 131 freez...
Article
STUDY QUESTION To what extent does a trophectoderm (TE) biopsy reliably reflect the chromosomal constitution of the inner cell mass (ICM) in human blastocysts? SUMMARY ANSWER Concordance between TE and ICM was established in 62.1% of the embryos analysed. WHAT IS KNOWN ALREADY Next generation sequencing (NGS) platforms have recently been optimise...
Article
Full-text available
Mouse embryonic stem cells (mESCs) exist in a naive, primed and ground state of pluripotency. While comparative analyses of these pluripotency states have been reported, the mESCs utilized originated from various genetic backgrounds and were derived in different laboratories. mESC derivation in conventional LIF + serum culture conditions is strain...
Article
Patients presenting with abnormally high numbers of immature oocytes at retrieval are more likely to exhibit maturation resistant oocytes. However, the clinical relevance of such events remains unknown. We investigated nuclear maturation competence of immature oocytes from patients showing >40% of collected immature oocytes (Study group) and Contro...
Article
STUDY QUESTION Can human oocyte calcium analysis predict fertilization success after assisted oocyte activation (AOA) in patients experiencing fertilization failure after ICSI? SUMMARY ANSWER ICSI-AOA restores the fertilization rate only in patients displaying abnormal Ca2+ oscillations during human oocyte activation. WHAT IS KNOWN ALREADY Patients...
Article
Inconsistent fertilisation and pregnancy rates have been reported by different laboratories after application of ionomycin as a clinical method of assisted oocyte activation (AOA) to overcome fertilisation failure. Using both mouse and human oocytes, in the present study we investigated the effects of ionomycin and Ca2+ concentrations on the patter...
Article
Oocyte activation is a calcium (Ca(2+)) dependent process that has been investigated in depth, in particular regarding its impact in assisted reproduction technology (ART). Following a standard model of signal transduction, Ca(2+) drives the meiotic progression upon fertilization in all species studied to date. However, Ca(2+) changes during oocyte...
Article
Background: Fertility preservation before or during cancer treatment in young women has become an important health issue because of delayed motherhood and improved survival rates. This study evaluates the necessity and the efficacy of fertility preservation, with a focus on actual pregnancy wish and outcome after fertility preservation and cancer...
Article
Although qualitative research about couples and families is becoming increasingly widespread, the aspect of data analysis remains largely underrepresented in the literature. In this methodological paper, we outline one specific approach to data analysis in the context of multi family member interview studies. Inspired by Interpretative Phenomenolog...
Article
Full-text available
Research has shown that the recipients of donor sperm can experience difficulties finding appropriate language to refer to the donor. Based on two qualitative analysis techniques, namely word count and empirical discourse analysis, we studied the words used to refer to the donor in heterosexual and lesbian (aspiring) parents and in donor conceived...
Article
Full-text available
Activation of the egg by the sperm is the first, vital stage of embryogenesis. The sperm protein PLCζ has been proposed as the physiological agent that triggers the Ca(2+) oscillations that normally initiate embryogenesis. Consistent with this, recombinant PLCζ induces Ca(2+) oscillations in eggs and debilitating mutations in the PLCZ1 gene are ass...
Article
Background: The use of nuclear transfer (NT) has been proposed as a novel reproductive treatment to overcome the transmission of maternally-inherited mitochondrial DNA (mtDNA) mutations. Pathogenic mutations in mtDNA can cause a wide-spectrum of life-limiting disorders, collectively known as mtDNA disease, for which there are currently few effecti...
Article
Although intra-familial egg donation has been practiced for more than 15 years in several countries, little is known about family relationships in this family type. Framed within the new kinship studies, this article focuses on the experiential dimension of kinship in sister-to-sister egg donation families: how is kinship ‘unpacked’ and ‘reconstruc...
Article
Full-text available
Background/aims A large proportion of men with normal sperm results as analyzed using conventional techniques have fragmented DNA in their spermatozoa. We performed a prospective study to examine the incidence of DNA fragmentation in sperm in cases of couples with previously unexplained infertility and treated with intrauterine insemination. We eva...
Article
Objective: To establish the value of array comparative genomic hybridization (CGH) for preimplantation genetic diagnosis (PGD) in embryos of translocation carriers in combination with vitrification and frozen embryo transfer in nonstimulated cycles. Design: Retrospective data analysis study. Setting: Academic centers for reproductive medicine...
Article
Full-text available
STUDY QUESTION Does prospective embryo selection using the results from the Eava Test (Early Embryo Viability Assessment) in combination with standard morphology increase the pregnancy rate of IVF and ICSI patients compared to embryo selection based on morphology only? SUMMARY ANSWER Embryo selection using the Eeva Test plus standard morphology on...
Article
Objective: To describe the decisions lesbian couples make when creating a family through donor insemination [DI] and to explore the negotiations between the biological and the non-biological mother. Methods: We included 18 lesbian parents (9 biological and 9 non-biological mothers) with at least one child (7-10 years old) conceived through anony...
Article
Full-text available
Objective cohort study describing clinical and laboratory outcomes in ICSI patients using self-operated endovaginal tele-monitoring (SOET). Setting University department of reproductive medicine. Patients 78 patients undergoing 100 consecutive ICSI attempts. Interventions patients’ recorded vaginal sonograms and sent recordings using the cloud b...
Article
Full-text available
Starting from only a few cells, current whole genome amplification (WGA) methods provide enough DNA to perform massively parallel sequencing (MPS). Unfortunately, all current WGA methods introduce representation bias which limits detection of copy number aberrations (CNAs) smaller than 3 Mb. A recent WGA method, called TruePrime single cell WGA, us...
Article
Full-text available
We present a fully defined culture system (adapted Essential8(TM) (E8(TM) ) medium in combination with vitronectin) for human embryonic stem cells (hESC) that can be used for Stable Isotopic Labeling of Amino acids for Cell culture (SILAC) purposes. Although a complete incorporation of the labels was observed after 4 days in culture, over 90% of pr...
Article
Full-text available
Background Several retrospective studies have evaluated seasonal variations in the outcome of IVF treatment. Some also included weather conditions, mostly temperature and hours of daylight. The results were conflicting. Methods In a retrospective study we analysed all fresh cycles (N = 9865) that were started between January 1, 2007 and December 3...
Article
Objective: This study aimed to gain an in-depth understanding of the experiences of genetic ties in intrafamily oocyte donation families. Background: Previous research has shown that most mothers have a good and stable relationship with their donor. Little is known about the meaning of the difference in genetic ties for parents who conceived throug...
Article
Full-text available
Background: Since the development of in vitro embryo production in cattle, different supplements have been added to culture media to support embryo development, with serum being the most popular. However, the addition of serum during embryo culture can induce high birthweights and low viability in calves (Large Offspring Syndrome). Analysis of glo...
Data
Rarefaction curves illustrating sampling depth in each endometrial sample
Data
Relative abundances of the 183 phylotypes in the distinct uterine communites (n = 19) Column A lists the unique phylotype numbers used throughout the manuscript and figures.Column C lists the corresponding taxonomical annotation. Columns D to V show the relative abundances of alle phylotypes per sample.
Data
Nucleotide sequences of all 183 phylotypes determined using Illumina-based amplicon deep-sequencing and their phylogenetic assignment Column A lists the phylotype numbers used to denote the 183 taxa throughout the manuscript and figures. Column B lists the sequences obtained on the Illumina MiSeq platform. Columns D to J show the taxonomic annotati...
Article
Findings on women’s sexuality across the menstrual cycle are inconsistent. One relatively consistent finding is a mid-cycle and premenstrual peak in sexual desire in freely cycling women. Results on the cycle-related effects of sexual behaviour are less clear. Large proportions of reproductive-aged women use combined oral contraception (COC), but s...
Article
Full-text available
Background. It is widely assumed that the uterine cavity in non-pregnant women is physiologically sterile, also as a premise to the long-held view that human infants develop in a sterile uterine environment, though likely reflecting under-appraisal of the extent of the human bacterial metacommunity. In an exploratory study, we aimed to investigate...
Article
Full-text available
Background. It is widely assumed that the uterine cavity in non-pregnant women is physiologically sterile, also as a premise to the long-held view that human infants develop in a sterile uterine environment, though likely reflecting under-appraisal of the extent of the human bacterial metacommunity. In an exploratory study, we aimed to investigate...
Article
In the absence of the maternal genital tract, preimplantation embryos can develop in vitro in culture medium where all communication with the oviduct or uterus is absent. In several mammalian species, it has been observed that embryos cultured in groups thrive better than those cultured singly. Here we argue that group-cultured embryos are able to...
Article
Hormonal and surgical treatments for transgender people have a devastating effect on the possibility for these patients to reproduce. Additionally, transgender people tend to start sex reassignment treatment at a young age, when reproductive wishes are not yet clearly defined nor fulfilled. The most recent Standards of Care of the World Professiona...
Article
Unlabelled: Background Research has highlighted the complex association between female sexual dysfunction (FSD) and distress regarding sexual activity, with decreased physical pleasure being an important mediator. The current study aims to elucidate the association between pleasurable and painful genital sensitivity and FSD, and to further investi...
Article
Full-text available
Variation in the level of mtDNA heteroplasmy in adult tissues is commonly seen in patients with a mixture of wild-type and mutant mtDNA. A mixture of different mtDNA variants may influence such variation and cause mtDNA segregation bias. We analyzed cellular heterogeneity in embryonic stem cells (ESCs) derived from a polymorphic mouse model contain...
Article
Objective: To assess the effect of two assisted oocyte activation (AOA) protocols with the use of two calcium (Ca(2+)) ionophores, ionomycin and A23187 (calcimycin), on the intracellular Ca(2+) level in mouse and human oocytes and the fertilization rates. Design: Comparison of two Ca(2+) ionophores, ionomycin and A23187, regarding their capacity...