Peter Vikesland

Peter Vikesland
Virginia Tech (Virginia Polytechnic Institute and State University) | VT · Department of Civil and Environmental Engineering

Ph.D. Civil and Environmental Engineering

About

207
Publications
48,518
Reads
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9,431
Citations
Additional affiliations
December 2001 - present
Virginia Tech (Virginia Polytechnic Institute and State University)
August 1999 - December 2001
Johns Hopkins University
August 1993 - December 1998
University of Iowa

Publications

Publications (207)
Preprint
Full-text available
The salinity of inland freshwaters is rising globally, particularly in human dominated landscapes that receive deicers in the winter. Here we show that the salinization of an urban stream in Northern Virginia (USA) can be linked to salt sources using stream water age as a master variable. Younger stream water is associated with either low salinity...
Article
Full-text available
Freshwater Salinization Syndrome (FSS) refers to groups of biological, physical, and chemical impacts which commonly occur together in response to salinization. FSS can be assessed by the mobilization of chemical mixtures, termed “chemical cocktails”, in watersheds. Currently, we do not know if salinization and mobilization of chemical cocktails al...
Article
Full-text available
Antibiotic resistance is of crucial interest to both human and animal medicine. It has been recognized that increased environmental monitoring of antibiotic resistance is needed. Metagenomic DNA sequencing is becoming an attractive method to profile antibiotic resistance genes (ARGs), including a special focus on pathogens. A number of computationa...
Article
A prompt on-site, real-time method to detect bacterial antibiotic resistance is crucial for controlling the spread of resistance. Herein, we report the use of surface-enhanced Raman spectroscopy (SERS) for the monitoring of bioactive metabolites produced by ampicillin-resistant Pseudomonas aeruginosa strains and identification of mechanisms underly...
Article
Full-text available
The Editors-in-Chief of the Environmental Science journals introduce the Best Papers of 2022.
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The Editors-in-Chief of the Environmental Science journals introduce the Best Papers of 2022.
Article
Full-text available
The Editors-in-Chief of the Environmental Science journals introduce the Best Papers of 2022.
Article
Full-text available
There are challenges in monitoring and managing water quality due to spatial and temporal heterogeneity in contaminant sources, transport, and transformations. We demonstrate the importance of longitudinal stream synoptic (LSS) monitoring, which can track combinations of water quality parameters along flowpaths across space and time. Specifically,...
Article
Full-text available
Efficient spread of respiratory viruses requires the virus to maintain infectivity in the environment. Environmental stability of viruses can be influenced by many factors, including temperature and humidity. Our study measured the impact of initial droplet volume (50, 5, and 1 μL) and relative humidity (RH; 40%, 65%, and 85%) on the stability of i...
Article
In situ spatiotemporal biochemical characterization of the activity of living multicellular biofilms under external stimuli remains a significant challenge. Surface-enhanced Raman spectroscopy (SERS), combining the molecular fingerprint specificity of vibrational spectroscopy with the hotspot sensitivity of plasmonic nanostructures, has emerged as...
Article
Label-free surface-enhanced Raman spectroscopy (SERS) has been proposed as a promising bacterial detection technique. However, the quality of the collected bacterial spectra can be affected by the time between sample acquisition and the SERS measurement. This study evaluated how storage stress stimuli influence the label-free SERS spectra of Pseudo...
Article
The Editors-in-Chief of the Environmental Science journals introduce the Best Papers of 2022.
Article
The Editors-in-Chief of the Environmental Science journals introduce the Best Papers of 2022.
Article
Full-text available
Surface-enhanced Raman spectroscopy (SERS) has great potential as an analytical technique for environmental analyses. In this study, we fabricated highly porous gold (Au) supraparticles (i.e., ∼100 μm diameter agglomerates of primary nano-sized particles) and evaluated their applicability as SERS substrates for the sensitive detection of environmen...
Article
Full-text available
Freshwater salinity is rising across many regions of the United States as well as globally, a phenomenon called the freshwater salinization syndrome (FSS). The FSS mobilizes organic carbon, nutrients, heavy metals, and other contaminants sequestered in soils and freshwater sediments, alters the structures and functions of soils, streams, and ripari...
Article
Full-text available
Multicellular systems, such as microbial biofilms and cancerous tumors, feature complex biological activities coordinated by cellular interactions mediated via different signaling and regulatory pathways, which are intrinsically heterogeneous, dynamic, and adaptive. However, due to their invasiveness or their inability to interface with native cell...
Article
Full-text available
The analysis of bacterial mobile genetic elements (MGEs) in genomic data is a critical step toward profiling the root causes of antibiotic resistance, phenotypic or metabolic diversity, and the evolution of bacterial genera. Existing methods for MGE annotation pose high barriers of biological and computational expertise to properly harness.
Article
Full-text available
Wastewater-based surveillance (WBS) for disease monitoring is highly promising but requires consistent methodologies that incorporate predetermined objectives, targets, and metrics. Herein, we describe a comprehensive metagenomics-based approach for global surveillance of antibiotic resistance in sewage that enables assessment of 1) which antibioti...
Article
Cellulose nanomaterials are low cost, biocompatible and readily combined with other materials to produce nanocomposites with a range of applications. Cellulose nanomaterials have many advantageous properties, including high mechanical and thermal stability, high specific surface area, and biodegradability. The highly flexible cellulose structure ca...
Article
Full-text available
The Editors-in-Chief of the Environmental Science journals introduce the Best Papers of 2021.
Article
An improved understanding of bacterial inactivation mechanisms will provide useful insights for infectious disease control and prevention. We evaluated bacterial response to several inactivation methods using surface-enhanced Raman spectroscopy (SERS). The results indicate that changes in the SERS signal are highly related to cellular disruption an...
Article
Full-text available
To evaluate the use of wastewater-based surveillance and epidemiology to monitor and predict SARS-CoV-2 virus trends, over the 2020-2021 academic year we collected wastewater samples twice weekly from 17 manholes across Virginia Tech's main campus. We used data from external door swipe card readers and student isolation/quarantine status to estimat...
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Microporous mesh plasmonic devices have the potential to combine the biocompatibility of microporous polymeric meshes with the capabilities of plasmonic nanostructures to enhance nanoscale light-matter interactions for bio-interfaced optical sensing and actuation. However, scalable integration of dense and uniformly structured plasmonic hotspot arr...
Article
Many outbreaks of emerging disease (e.g., avian influenza, SARS, MERS, Ebola, COVID-19) are caused by viruses. In addition to direct person-to-person transfer, the movement of these viruses through environmental matrices...
Article
Antimicrobial resistance (AMR) is a growing global health threat that requires coordinated action across One Health sectors (humans, animals, environment) to stem its spread. Environmental surveillance of AMR is largely behind the curve in current One Health surveillance programs, but recent momentum in the establishment of infrastructure for monit...
Article
The functional profile of metagenomic samples enables improved understanding of microbial populations in the environment. Such analysis consists of assigning short sequencing reads to a particular functional category. Normally, manually curated databases are used for functional assignment, and genes are arranged into different classes. Sequence ali...
Preprint
Full-text available
Currently available databases of bacterial mobile genetic elements (MGEs) contain both core and accessory MGE functional modules, the latter of which are often only transiently associated with the element. The presence of these accessory genes, which are often close homologs to primarily immobile genes, limits the usability of these databases for M...
Article
Full-text available
Inland freshwater salinity is rising worldwide, a phenomenon called the freshwater salinization syndrome (FSS). We investigate a potential conflict between managing the FSS and indirect potable reuse, the practice of augmenting water supplies through the addition of highly treated wastewater (reclaimed water) to surface waters and groundwaters. Fro...
Chapter
The functional profile of metagenomic samples allows the understanding of the role of the microbes in the environment. Sequence alignment of short reads against curated databases has been widely used to profile metagenomic samples. However, this method is time consuming and requires high computing resources. Although several alignment free methods...
Article
The impacts of the ongoing coronavirus pandemic highlight the importance of environmental monitoring to inform public health safety. Wastewater based epidemiology (WBE) has drawn interest as a tool for analysis of biomarkers in wastewater networks. Wide scale implementation of WBE requires a variety of field deployable analytical tools for real-tim...
Preprint
Wastewater-based epidemiology (WBE) for disease monitoring is highly promising, but requires consistent methodologies that incorporate predetermined objectives, targets, and metrics. We demonstrate a comprehensive metagenomics-based approach for global surveillance of antibiotic resistance in sewage, enabling assessment of: 1) which antibiotic resi...
Article
Full-text available
Wastewater treatment plants (WWTPs) receive a confluence of sewage containing antimicrobials, antibiotic resistant bacteria, antibiotic resistance genes (ARGs), and pathogens and thus are a key point of interest for antibiotic resistance surveillance. WWTP monitoring has the potential to inform with respect to the antibiotic resistance status of th...
Article
Full-text available
Ambient temperature and humidity strongly affect inactivation rates of enveloped viruses, but a mechanistic, quantitative theory of these effects has been elusive. We measure the stability of SARS-CoV-2 on an inert surface at nine temperature and humidity conditions and develop a mechanistic model to explain and predict how temperature and humidity...
Article
River networks are one of the main routes by which the public could be exposed to environmental sources of antibiotic resistance, that may be introduced e.g. via treated wastewater. In this study, we applied a comprehensive integrated analysis encompassing mass-flow concepts, chemistry, bacterial plate counts, resistance gene quantification and sho...
Article
Full-text available
In the fight to limit the global spread of antibiotic resistance, the assembly of environmental metagenomes has the potential to provide rich contextual information (e.g., taxonomic hosts, carriage on mobile genetic elements) about antibiotic resistance genes (ARG) in the environment. However, computational challenges associated with assembly can i...
Article
The emergence of next generation sequencing (NGS) is revolutionizing the potential to address complex microbiological challenges in the water industry. NGS technologies can provide holistic insight into microbial communities and their functional capacities in water and wastewater systems, thus eliminating the need to develop a new assay for each ta...
Article
Suspect screening using liquid chromatography with high resolution mass spectrometry provides an opportunity for expanding the detection coverage of emerging contaminants in the environment. Screening workflows suffer from high frequency of false positives or insufficient confidence in the identification of compounds, however, stringent criteria co...
Article
Ammonium is an important atmospheric constituent that dictates many environmental processes. The impact of the ammonium ion concentration on 10-50 μm aerosol droplet pH was quantified using pH nanoprobes and surface-enhanced Raman spectroscopy (SERS). Sample solutions were prepared by mixing 1 M ammonium sulfate (AS), ammonium nitrate (AN), sodium...
Article
Comprehensive surveillance approaches are needed to assess sources, clinical relevance, and mobility of antibiotic resistance genes (ARGs) in watersheds. Here, we examined metrics derived from shotgun metagenomic sequencing and relationship to human fecal markers (HFMs; crAssphage, enterococci) and anthropogenic antibiotic resistance markers (AARMs...
Article
Since emerging in late 2019, SARS-CoV-2 has caused a global pandemic, and it may become an endemic human pathogen. Understanding the impact of environmental conditions on SARS-CoV-2 viability and its transmission potential is crucial to anticipating epidemic dynamics and designing mitigation strategies. Ambient temperature and humidity are known to...
Preprint
Full-text available
Inland freshwater salinity is on the rise in many regions across the globe—a phenomenon called the freshwater salinization syndrome (FSS). In this paper we investigate a potential conflict between managing the FSS and indirect potable reuse, the practice of augmenting water supplies through the addition of reclaimed wastewater to surface waters and...
Article
Bacterial cellulose nanocrystals (BCNCs) are biocompatible cellulose nanomaterials that can host guest nanoparticles to form hybrid nanocomposites with a wide range of applications. Herein, we report the synthesis of a hybrid nanocomposite that consists of gold (AuNP) and iron oxide (Fe3O4) nanoparticles supported on BCNCs (Au@Fe3O4@BCNCs). As a pr...
Article
Motivation: Curation of antibiotic resistance gene (ARG) databases is labor intensive and requires expert knowledge to manually collect, correct, and/or annotate individual genes. Consequently, most existing ARG databases contain only a small number of ARGs (∼5k genes) and updates to these databases tend to be infrequent, commonly requiring years...
Article
4-mercaptopyridine (4-Mpy) is a pH reporter molecule commonly used to functionalize nanoprobes for surface-enhanced Raman spectroscopy (SERS) based pH measurements. However, nanoprobes functionalized by 4-Mpy alone have low pH sensitivity and are subject to interference by halide ions in sample media. To improve nanoprobe pH sensitivity and reliabi...
Article
Full-text available
Background: The interconnectivities of built and natural environments can serve as conduits for the proliferation and dissemination of antibiotic resistance genes (ARGs). Several studies have compared the broad spectrum of ARGs (i.e., "resistomes") in various environmental compartments, but there is a need to identify unique ARG occurrence pattern...
Article
Full-text available
Background: Direct and indirect selection pressures imposed by antibiotics and co-selective agents and horizontal gene transfer are fundamental drivers of the evolution and spread of antibiotic resistance. Therefore, effective environmental monitoring tools should ideally capture not only antibiotic resistance genes (ARGs), but also mobile genetic...
Article
There is a need to improve understanding of the effect of chlorine disinfection on antibiotic resistance genes (ARGs) in order to advance relevant drinking water, wastewater, and reuse treatments. However, few studies have explicitly assessed the physical effects on the DNA. Here we examined the effects of free chlorine (1–20 mg Cl2/L) on extracell...
Article
Full-text available
Complex subsurface contamination domains and limited efficacy of existing treatment approaches pose significant challenges to site remediation and underscore the need for technological innovation to develop cost-effective remedies. Here, we discuss opportunities for nanotechnology-enabled in situ remediation technologies to address soil and groundw...
Article
Nanomaterials in the Earth system Nanomaterials have been part of the Earth system for billions of years, but human activities are changing the nature and amounts of these materials. Hochella Jr. et al. review sources and impacts of natural nanomaterials, which are not created directly through human actions; incidental nanomaterials, which form uni...
Article
The in situ characterization of organic pollutant dynamics on engineered interfaces provides fundamental insights about pollutant fate, transport, and toxicity in aquatic systems. In this study, the association between aromatic amines and citrate-coated gold nanoparticles (cit-AuNPs) under different pH conditions was monitored in situ using hot spo...
Article
Antimicrobial resistance (AMR) is one of the greatest threats faced by humankind. The development of resistance in clinical and hospital settings has been well documented ever since the initial discovery of penicillin and the subsequent introduction of sulfonamides as clinical antibiotics. In contrast, the environmental (i.e., community-acquired) d...
Preprint
Full-text available
The functional profile of metagenomic samples allows the understanding of the role of the microbes in their environment. Such analysis consists of assigning short sequencing reads to a particular functional category. Normally, manually curated databases are used for functional assignment where genes are arranged into different classes. Sequence ali...
Preprint
Full-text available
Curation of antibiotic resistance gene (ARG) databases is labor intensive and requires expert knowledge to manually collect, correct, and/or annotate individual genes. Consequently, most existing ARG databases contain only a small number of ARGs (~5k genes) and updates to these databases tend to be infrequent, commonly requiring years for completio...
Article
Although nitrification is widely applied for nitrogen removal in wastewater treatment plants (WWTPs), more information about the microorganisms involved and their corresponding capabilities and limitations is critical to refine kinetic parameters and process design to optimize advanced nutrient removal. Here we carried out a series of ammonia starv...
Article
Antimicrobial resistance is a worldwide problem that is both pressing and challenging due to the rate at which it is spreading, and the lack of understanding of the mechanisms that link human, animal and environmental sources contributing to its proliferation. One knowledge gap that requires immediate attention is the significance of antimicrobial...
Article
Nanoparticle surface coatings dictate their fate, transport, and bioavailability. We used a gold nanoparticle-bacterial cellulose substrate and “hot spot”-normalized surface-enhanced Raman scattering (HSNSERS) to achieve in situ and real-time monitoring of ligand exchange reactions on the gold surface. This approach enables semi-quantitative determ...
Preprint
Full-text available
Direct selection pressures imposed by antibiotics, indirect pressures by co-selective agents, and horizontal gene transfer are fundamental drivers of the evolution and spread of antibiotic resistance. Therefore, effective environmental monitoring tools should ideally capture not only antibiotic resistance genes (ARGs), but also mobile genetic eleme...
Article
Full-text available
The need and desire to understand the environment, especially the quality of one's local water and air, has continued to expand with the emergence of the digital age. The bottleneck in understanding the environment has switched from being able to store all of the data collected to collecting enough data on a broad range of contaminants of environme...
Article
The integration of surface enhanced Raman scattering (SERS) with droplet microfluidics has the potential to improve our understanding of cellular systems. Herein, we present the first application of SERS droplet microfluidics for single cell analysis. A microfluidic device was used to encapsulate single prostate cancer cells and wheat germ agglutin...
Article
Nanomaterial-enabled sensors are being designed for high-efficiency, multiplex-functionality and high-flexibility sensing applications. Many existing nanosensors have the inherent capacity to achieve such goals; however, they require further development into consumer- and operator-friendly tools with the ability to detect analytes in previously ina...
Article
Full-text available
Significance Aerosols with high water content (aerosol droplets) are ubiquitous and play a significant role in atmospheric chemistry and meteorology. However, directly measuring the pH of an individual aerosol droplet remains challenging due to its inaccessibility to pH electrodes. In this study, nanometer-sized pH probes were dispersed in droplets...